|Budget Amount *help
¥235,170,000 (Direct Cost: ¥180,900,000、Indirect Cost: ¥54,270,000)
Fiscal Year 2013: ¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2012: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
Fiscal Year 2011: ¥52,000,000 (Direct Cost: ¥40,000,000、Indirect Cost: ¥12,000,000)
Fiscal Year 2010: ¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
Fiscal Year 2009: ¥52,000,000 (Direct Cost: ¥40,000,000、Indirect Cost: ¥12,000,000)
Three kinds of structural research relating to the transportation were carried out. The first is the nuclear export mechanism of pre-miRNA. The second is structural study of vault that carries materials in the cell. The third is structural-functional studies of gap junction channels.
Structures of Exportin-5:RanGTP:Pre-miRNA complex, Exportin-5:RanGTP complex and Exportin-5 were determined at respective 2.9, 3.0 and 3.5 A resolutions. Comparing structures of exportin-5s in three different states and analyzing interactions between Pre-miRNA and Exportin-5, we elucidated Pre-miRNA transportation mechanism by Exportin-5:RanGTP complex. We have determined the 39-fold symmetry for the MVP-vault in addition to the vault from rat liver. Consequently, the 39-fold symmetry of vault was confirmed. We elucidated docking selectivity rules for hemichannel pairs by homology modeling based on the connexin-26 gap junction channel and functional analyses.