| Budget Amount *help |
¥208,000,000 (Direct Cost: ¥160,000,000、Indirect Cost: ¥48,000,000)
Fiscal Year 2013: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2012: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2011: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2010: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2009: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
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| Research Abstract |
In mouse germ cell development, the activation of an RNA binding protein Nanos2 is a critical step for male germ cell differentiation. We found that TGF-b signal (nodal/activin) mediated by Smad2 signaling is required for the Nanos2 induction. Once Nanos2 is induced, it localizes to P-bodies and interacts with CNOT complex, by which target RNAs are degraded. Nanos2 suppresses female pathway and promotes male pathway by repressing RNAs involved in the maintenance of PGCs, meiosis and mitosis in embryonic gonads. Nanos2 is also expressed in spermatogonial stem cells and plays an important role for the maintenance of stem cells. In the absence of Nanos2, stem cells can not maintain the stem cell property and quickly differentiate. We found that coordination of stem cells between self-renewal and differentiation is regulated by both extrinsic niche signals such as GDNF, FGF, RA and stem cell intrinsic factors such as Nanos2.
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