|Budget Amount *help
¥203,190,000 (Direct Cost: ¥156,300,000、Indirect Cost: ¥46,890,000)
Fiscal Year 2013: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2012: ¥37,310,000 (Direct Cost: ¥28,700,000、Indirect Cost: ¥8,610,000)
Fiscal Year 2011: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000)
Fiscal Year 2010: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000)
Fiscal Year 2009: ¥63,180,000 (Direct Cost: ¥48,600,000、Indirect Cost: ¥14,580,000)
Based on ecophysiological and environmental information on the wild eels, the methods of artificial maturation of the freshwater eel and the rearing techniques of larvae were improved. Transcriptome analysis revealed that mRNA expressions of ara, aqp0, aqp3-like genes were significantly higher in naturally maturing eels than artificially matured ones, which can be used as markers for obtaining high quality eggs in artificially matured eels. According to the analyses for osmotic and nutritional homeostasis in eel larvae, ingested seawater and intestinal NKCC2b/NCCb expressions were observed after 2 days post-hatching, and PEPT1 was localized on the apical region of intestinal epithelial cells after 5 days post-hatching. These results contribute to further improvement of rearing conditions for eel larvae. A new type of larval diet has been developed based on the marine snow that was found to be food for wild larvae by the stable isotopic analysis of amino acid nitrogen of larval body.