|Budget Amount *help
¥238,940,000 (Direct Cost: ¥183,800,000、Indirect Cost: ¥55,140,000)
Fiscal Year 2013: ¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
Fiscal Year 2012: ¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
Fiscal Year 2011: ¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000)
Fiscal Year 2010: ¥56,810,000 (Direct Cost: ¥43,700,000、Indirect Cost: ¥13,110,000)
Fiscal Year 2009: ¥50,960,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥11,760,000)
We searched for previously unidentified functions of intracellular Ca2+ signals in the central nervous system (CNS) using imaging methods for signaling molecules, and obtained the following results as regard the network mechanism in the CNS. (1) We discovered astrocytic Ca2+ signaling-mediated mechanism for astrogliosis and neuroprotection. (2) We succeeded in the visualization of spatiotemporal dynamics of glutamate signaling. This study was extended to in vivo glutamate imaging, and proved that sensory input induces glutamate spillover. (3) We discovered NO-induced Ca2+ release mechanism (NICR). NICR is involved in the long-term potentiation in the cerebellar cortex and in NO-mediated neuronal cell death.