• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of analytical system for exhaled breath and clinical application

Research Project

Project/Area Number 21240057
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical systems
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SHIMOUCHI Akito  独立行政法人国立循環器病研究センター, 研究所, 室長 (80211291)

Co-Investigator(Kenkyū-buntansha) KONDO Takaharu  中部大学, 生命健康科学部, 教授 (20135388)
Co-Investigator(Renkei-kenkyūsha) MIZOKAMI Takaharu  (株)日本エイピーアイ, 代表取締役社長 (40520630)
Project Period (FY) 2009-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
Keywords呼気 / 質量分析 / 生活習慣 / 分析法 / 生体ガス / 低分子ガス / 呼気スペクトラム / 生体ガス処理 / 非侵襲的診断 / 自己組織化マップ / 高感度呼気分析 / 大気圧イオン化質量分析 / 呼吸回路 / 生体ガス濃縮 / 呼気診断
Outline of Final Research Achievements

Chemical compounds in breath originate mainly from volatile metabolic products in health and diseases. Fasted 1011 subjects aged from 20 to 88 years old volunteered for the study. Minute ventilation volumes of carbon dioxide (CO2), oxygen (O2), hydrogen (H2), methane (CH4), carbon monoxide (CO), nitric oxide (NO) and nitrogen oxide (NOX) were measured by the standard methods. VO2, VCO2 and VH2 were significantly decreased in age-dependent manner. Contrarily, VCH4 was increased in aged subjects. VCO was significantly increased to aging especially in women, while VNO did not significantly changed. Significant increases in VCO and decrease in VNO were observed in heavy smokers. VCO was increased in the 4th week after the starting date of menstruation. Futhermore, we developed the analytical method for non-invasive direct measurement of OH radicals emanated from the human skin, indirect methods by ingestion or inhalation of H2 and convienent breath H2 analyser.

Report

(6 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (54 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (24 results) Book (2 results) Remarks (4 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] 呼気ガス医療診断の現状と課題,今後の展望2014

    • Author(s)
      下内章人, 近藤孝晴
    • Journal Title

      応用物理

      Volume: 83 Pages: 26-32

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular hydrogen consumption in the human body during the inhalation of hydrogen gas2013

    • Author(s)
      Shimouchi A, Nose K, Mizukami T, Che DC, Shirai M.
    • Journal Title

      Adv Exp Med Biol

      Volume: 789 Pages: 315-321

    • DOI

      10.1007/978-1-4614-7411-1_42

    • ISBN
      9781461472568, 9781461474111
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Emanation of hydroxyl radicals from human skin2013

    • Author(s)
      Che DC, Shimouchi A, Mizukami T, Nose K, Seiyama A, Kasai T.
    • Journal Title

      IEEE Sensors J

      Volume: 13 Pages: 1223-1227

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-Throughput Separation Assay for NO Metabolites in Blood Using Microfluidic Electrophoresis2013

    • Author(s)
      Wakida S, Miyado T, Shimazu K, ShibutaniY, Mizukami T, Nose K, Shimouchi A
    • Journal Title

      ECS Trans

      Volume: 50 Pages: 165-170

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 携帯型呼気水素計測器の開発と臨床使用経験2013

    • Author(s)
      利川寶,下内章人,池田大祐,鈴木登,大槻知佐,木村郁彦
    • Journal Title

      安定同位体と生体ガス

      Volume: 5 Pages: 7-13

    • NAID

      40020354260

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insufflation of hydrogen gas restrains the inflammatory response of cardiopulmonary bypass in a rat model.2013

    • Author(s)
      Fujii Y, Shirai M, Inamori S, Shimouchi A, Sonobe T, Tsuchimochi H, Pearson JT, Takewa Y, Tatsumi E, Taenaka Y.
    • Journal Title

      Artif Organs.

      Volume: 37 Issue: 2 Pages: 136-141

    • DOI

      10.1111/j.1525-1594.2012.01535.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] H(2) mediates cardioprotection via involvements of K(ATP) channels and permeability transition pores of mitochondria in dogs2012

    • Author(s)
      Yoshida A, Asanuma H, Sasaki H, Sanada S, Yamazaki S, Asano Y, Shinozaki Y, Mori H, Shimouchi A, Sano M, Asakura M, Minamino T, Takashima S, Sugimachi M, Mochizuki N, Kitakaze M.
    • Journal Title

      Cardiovasc Drugs Ther.

      Volume: 26 Issue: 3 Pages: 217-226

    • DOI

      10.1007/s10557-012-6381-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of molecular hydrogen consumption in the human whole body after the ingestion of hydrogen-rich water2012

    • Author(s)
      Shimouchi A, Nose K, Shirai M, Kondo T.
    • Journal Title

      Adv Exp Med Biol.

      Volume: 737 Pages: 245-250

    • DOI

      10.1007/978-1-4614-1566-4_36

    • ISBN
      9781461415657, 9781461415664
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of molecular hydrogen consumption in the human whole body after the ingestion of hydrogen-rich water2012

    • Author(s)
      Shimouchi A, Nose K, Shirai M, Kondo T
    • Journal Title

      Adv Exp Med Biol

      Volume: 737 Pages: 245-250

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Rho-kinase signaling and endothelial dysfunction in modulating blood flow distribution in pulmonary hypertension2011

    • Author(s)
      Schwenke DO, Pearson JT, Sonobe T, Ishibashi-Ueda H, Shimouchi A, Kangawa K, Umetani K, Shirai M
    • Journal Title

      J Appl Physiol

      Volume: 110 Pages: 901-908

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring breath hydrogen using thermoelectric sensor2011

    • Author(s)
      Shin W, Nishibori N, Izu N, Itoh T., Matsubara I, Nose K, Shimouchi A
    • Journal Title

      Sensor Letters

      Volume: 9 Pages: 1-4

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A tracer analysis study on the distribution and oxidization of endogenous carbon monoxide in the human body.:, 20102010

    • Author(s)
      Sawano M, Shimouchi A.
    • Journal Title

      J Clin Biochem Nutr

      Volume: 47 Pages: 107-110

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Factors relating to life satisfaction and subjective healthy feelings in respiratory patients during home oxygen therapy.2010

    • Author(s)
      Morimoto T., Shimouchi A.
    • Journal Title

      J Info Behav health Welfare

      Volume: 1 Pages: 5-12

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Breath and Skin Gases Emanating during Exercise using an Original Biogas Sampling System connected to an Atmospheric Pressure lonization Mass Spectrometer2010

    • Author(s)
      Shimouchi A, Nose K, Shirai M.
    • Journal Title

      IEEE Sensors Journal

      Volume: 10 Pages: 85-91

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 水素ガス吸入における皮膚ガス水素の動態2010

    • Author(s)
      野瀬和利, 下内章人, 山口誠, 石黒洋, 近藤孝晴
    • Journal Title

      安定同位体と生体ガス

      Volume: 2(1) Pages: 35-39

    • NAID

      40018999097

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 呼気中アセトン濃度の変動についての基礎データの検討2010

    • Author(s)
      垣津奈美, 永峰康一郎, 近藤孝晴, 下内章人, 野瀬和利, 加賀屋みえ子
    • Journal Title

      安定同位体と生体ガス

      Volume: 2(1) Pages: 40-46

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Breath and Skin Gases Emanating during Exercise using an Original Biogas Sampling System connected to an Atmospheric Pressure Ionization Mass Spectrometer.2010

    • Author(s)
      Shimouchi A, Nose K, Shirai M
    • Journal Title

      IEEE Sensors Journal 10

      Pages: 85-91

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Breath hydrogen produced by ingestion of commercial hydrogen water and milk.2009

    • Author(s)
      Shimouchi A, Nose K, Yamaguchi M, Ishiguro H, Kondo T.
    • Journal Title

      Biomarker Insights 4

      Pages: 27-32

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of dietary turmeric on breath hydrogen2009

    • Author(s)
      Shimouchi A, Nose K, Takaoka M, Hayashi H, Kondo T.
    • Journal Title

      Dig Dis Sci 54

      Pages: 1725-1729

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apical Cl^-/HCO_3^-exchanger stoichiometry in the modeling of HCO_3^- transport by pancreatic duct epithelium.2009

    • Author(s)
      Yamaguchi M, Ishiguro H, Steward M, Sohma Y, Yamamoto A, Shimouchi A, Kondo T.
    • Journal Title

      J.Med.Invest. 56

      Pages: 25-328

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Breath hydrogen may be an anti-oxidative biomarker2014

    • Author(s)
      Akito Shimouchi, Tomoe Mizukami, Naoya Jinno, Kentaro Taniguchi, Kazutoshi Nose, Takaharu Kondo
    • Organizer
      17th Biennial Meeting of Society for Free Radical International
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hydrogen Consumption in the Human Body during the Inhalation of Hydrogen Gas2013

    • Author(s)
      Akito Shimouchi, Kazutoshi Nose, Tomoe Mizukami, Dock-Chil Che, Mikiyasu Shirai
    • Organizer
      日本呼吸器学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Possible clinical significance of breath hydrogen monitoring as an anti-oxidative biomarker2013

    • Author(s)
      Shimouchi A, Kondo T.
    • Organizer
      Breath Analysis Summit 2013
    • Place of Presentation
      Saarbruecken, Germany
    • Related Report
      2013 Annual Research Report
  • [Presentation] Increases in Breath Carbon Oxide during Night Sleep are associated with Deterioration of Mental Health Conditions2013

    • Author(s)
      Akito Shimouchi, Tomoe Mizukami, Kazutoshi Nose, Kentaro Taniguchi.
    • Organizer
      日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水素水飲水ならびに低濃度水素ガス吸入に伴う水素消費の定量2013

    • Author(s)
      下内章人,水上智恵,野瀬和利,蔡徳七,白井幹康,近藤孝晴
    • Organizer
      分子状水素医学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水素水摂取に伴う水素消失と生体内活性酸素種生成量の間接的推定2012

    • Author(s)
      下内章人
    • Organizer
      日本衛生学会シンポジウム
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 呼気水素の日内変動と携帯型呼気水素計測器の使用経験2012

    • Author(s)
      下内章人,細田享平,水上智恵, 野瀬和利,利川寶
    • Organizer
      第4回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 有精鶏卵の卵殻膜気孔におけるガス透過性の分子量依存性2012

    • Author(s)
      下内章人,水上智恵,野瀬和利
    • Organizer
      日本分析化学会
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水素水を利用した生体内活性酸素種生成の間接的定量法の開発2012

    • Author(s)
      下内章人,野瀬和利,水上智恵,白井幹康,近藤孝晴
    • Organizer
      日本酸化ストレス学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular Hydrogen Consumption in the Human Whole Body during Inhalation of Hydrogen Gas2012

    • Author(s)
      Shimouchi A, Nose K, Mizukami T, Che D-C, Shirai M, Kondo T
    • Organizer
      International Society of Oxygen Transport to Tissues
    • Place of Presentation
      ベルギー
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水素水飲水に伴う生体内水素消費量の計測2011

    • Author(s)
      下内章人, 野瀬和利, 水上智恵, 白井幹康, 近藤孝晴
    • Organizer
      日本安定同位体生体ガス医学応用学会
    • Place of Presentation
      東邦大学(東京)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生体と雰囲気のマスバランスを考慮した呼気微量ガス成分の評価法2011

    • Author(s)
      下内章人, 野瀬和利, 水上智恵
    • Organizer
      日本分析化学会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Estimation of Molecular Hydrogen Consumption in the Human Whole Body after the Ingestion of Hydrogen-Rich Water2011

    • Author(s)
      Akito Shimouchi, Kazutoshi Nose, Tomoe Mizukami, Mikiyasu Shirai, Takaharu Kondo
    • Organizer
      第15回酸素ダイナミクス研究会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Year and Date
      2011-09-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] ウコン含有食品の呼気水素上昇作用2011

    • Author(s)
      下内章人
    • Organizer
      日本酸化ストレス学会
    • Place of Presentation
      北海道
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸内嫌気性醗酵に伴う水素分子の抗酸化ストレス機能2011

    • Author(s)
      下内章人
    • Organizer
      日本酸化ストレス学会シンポジウム
    • Place of Presentation
      北海道
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Variabilities of physicochemical characteristics of the skin conditions in humans2011

    • Author(s)
      Kawamura A, Nose K, Hayashi H, Nishi N, Shirai M, Shimouchi A.
    • Organizer
      日本生理学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 水素ガス吸入における皮膚ガス水素の動態2010

    • Author(s)
      野瀬和利, 下内章人, 山口誠, 石黒洋, 近藤孝晴
    • Organizer
      第2回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 運動による呼気中アセトン濃度の変動2010

    • Author(s)
      垣津奈美, 永峰康一郎, 近藤孝晴, 下内章人, 野瀬和利, 加賀谷みえ子
    • Organizer
      第2回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 自由行動下における呼気水素変動に関する探索的事例検討2010

    • Author(s)
      細田享平, 岩本健司, 野瀬和利, 下内章人
    • Organizer
      第2回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 内因性COの組織間信号伝達に関する13COを用いた基礎的検討2010

    • Author(s)
      澤野誠, 下内章人
    • Organizer
      第2回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 乳製品嗜好・牛乳摂取による呼気水素と酸化ストレスの関係2010

    • Author(s)
      下内章人, 野瀬和利, 近藤孝晴
    • Organizer
      第2回日本安定同位体・生体ガス医学応用学会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] A preliminary study of the influence of aerobic exercises on breath acetone2010

    • Author(s)
      Koichiro Nagamine, Nami Kakitsu, Takaharu Kondo, Akito Shimouchi, Kazutoshi Nose
    • Organizer
      Exhaled Breath Analysis : From Sensors to Devices and Applications
    • Place of Presentation
      Barga, Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] Estimation of oxygen radical production in the human whole body by molecular hydrogen consumption after the Ingestion of hydrogen-rich water2010

    • Author(s)
      Shimouchi A, Nose K, Shirai M, Kondo T
    • Organizer
      The International Society on 02 Transport to Tissue 2010
    • Place of Presentation
      Ascona, Switzerland
    • Related Report
      2010 Annual Research Report
  • [Presentation] 運動負荷に伴う呼気・皮膚ガス微量成分の動態2010

    • Author(s)
      下内章人, 野瀬和利, 白井幹康
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      岩手県盛岡市
    • Related Report
      2010 Annual Research Report
  • [Book] 「からだと酸素の事典」酸素ダイナミクス研究会編(質量分析)2009

    • Author(s)
      野瀬和利, 下内章人
    • Total Pages
      4
    • Publisher
      朝倉書店
    • Related Report
      2009 Annual Research Report
  • [Book] 「からだと酸素の事典」酸素ダイナミクス研究会編(低酸素性肺血管攣縮)2009

    • Author(s)
      下内章人, 白井幹康
    • Total Pages
      3
    • Publisher
      朝倉書店
    • Related Report
      2009 Annual Research Report
  • [Remarks] 心臓生理機能部 生体ガスによる非侵襲的診断法の開発と臨床応用

    • URL

      http://www.ncvc.go.jp/res/divisions/cardiac_physiology/studium_b.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 国立循環器病研究センター

    • URL

      http://www.ncvc.go.jp/res/divisions/cardiac_physiology/studium_b.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/divisions/cardiac_physiology/cp_003.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.ncvc.go.jp/res/divisions/cardiac_physiology/cp_003.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体内活性酸素種の測定法2012

    • Inventor(s)
      下内章人,野瀬和利,水上智恵,利川寶
    • Industrial Property Rights Holder
      独立行政法人国立循環器病研究センター,株式会社タイヨウ
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-171570
    • Filing Date
      2012-08-04
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 体表ガス中の活性酸素測定装置及び測定方法2012

    • Inventor(s)
      蔡徳七, 下内章人, 溝上員章
    • Industrial Property Rights Holder
      大阪大学学長名, 国立循環器病研究センター総長名, 株式会社日本エイピーアイ
    • Industrial Property Number
      2012-071189
    • Filing Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体内活性酸素推定法2010

    • Inventor(s)
      下内章人, 野瀬和利
    • Industrial Property Rights Holder
      ヒューマンサイエンス振興財団
    • Industrial Property Number
      2010-235771
    • Filing Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 腸内醗酵抑制剤2009

    • Inventor(s)
      下内章人, 野瀬和利, 利川寶
    • Industrial Property Rights Holder
      国立循環器病センター総長名利川寶
    • Industrial Property Number
      2009-118745
    • Filing Date
      2009-05-14
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi