| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2011: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2010: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2009: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
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| Research Abstract |
In this project, we aimed at creating new photo-driven DNA machines with photo-responsive DNA involving azobenzenes. First, we designed a newly modified azobenzene that enables efficient photo-regulation of DNA hybridization. We found that modification of a distal benzene ring at two ortho-positions was effective for the improvement of both photoregulatory efficiency and thermal stability of cis-form. Furthermore, we also clarified that the modified duplex of alternating base-pair and azobenzene residues allowed complete photo-regulation of DNA hybridization only by light-irradiation. Based on these results, we successfully constructed 1) photo-driven DNA machine that can reversibly digest target RNA, 2) photo-degradable DNA nano-capsule, and 3) DNA machine that can move like seesaw by the combination with visible light-responsive azobenzene.
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