Mammalian organ regeneration method using the roadmap for amphibian organogenesis.

• Project/Area Number: 21241048
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: The University of Tokyo
• Principal Investigator: ASASHIMA Makoto 東京大学, 大学院・総合文化研究科, 名誉教授 (00090564)
• Co-Investigator(Kenkyū-buntansha): ITO Yuzuru 産業技術総合研究所, 幹細胞工学研究センター, 研究チーム長 (30500079) ; ARIIZUMI Takashi 玉川大学, 農学部, 教授 (30286166) ; 栗崎 晃 産業技術総合研究所, 器官発生工学研究ラボ, 主任研究員 (60346616)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000); Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2009: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
• Keywords: 臓器 / 幹細胞 / 分化 / 再生 / ES細胞 / 心臓

Research Abstract

The establishment of efficient methods for promoting stem cell differentiation into target cells is important in regenerative medicine. Although various stem cells, such as ES and iPS cells, are being described, there remains a lack of protocols for inducing the differentiation of these cells. Based on the in vitro organogenesis methods using Xenopus, we have identified many "roadmap" genes that are indispensable for normal development in various organs. We have applied the roadmap of the amphibian organogenesis to mammalian stem cells, so as to facilitate the development of efficient methodologies for in vitro differentiation.

Research Products

• [Journal Article] Ubc9 negatively regulates BMP-mediated osteoblastic differentiation in cultured cells (2012)
  • Author(s): Yukita A, Hosoya A, Ito Y, Katagiri T, Asashima M, Nakamura H
  • Journal Title: Bone Volume: 50 Issue: 5 Pages: 1092-1099
  • DOI: 10.1016/j.bone.2012.02.008
  • Peer Reviewed
• [Journal Article] The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme (2011)
  • Author(s): Yoshiyama-Yanagawa T, Enya S, Shimada-Niwa Y, Yaguchi S, Haramoto Y, Matsuya T, Shiomi K, Sasakura Y, Takahashi S, Asashima M, Kataoka H, Niwa R
  • Journal Title: J Biol Chem Volume: 286 Pages: 25756-25762
  • Peer Reviewed
• [Journal Article] Glycome diagnosis of human induced pluripotent stem cells using lectin microarray (2011)
  • Author(s): Tateno H., Toyoda M., Saito S., Onuma Y., Ito Y., Hiemori K., Fukumura M., Nakasu A., Nakanishi M., Ohnuma K., Akutsu H., Umezawa A., Horimoto K., Hirabayashi J., Asashima M
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 20345-20353
  • Peer Reviewed
• [Journal Article] Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb (2011)
  • Author(s): Kuwabara T., Kagalwala M. N., Onuma Y., Ito Y., Warashina M., Terashima K., Sanosaka T., Nakashima K., Gage F. H., Asashima, M
  • Journal Title: EMBO Mol. Med Volume: 3 Pages: 742-754
  • Peer Reviewed
• [Journal Article] Development of Ca(2)(+) signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation (2011)
  • Author(s): Takano K., Obata S., Komazaki S., Masumoto M., Oinuma T., Ito Y., Ariizumi T., Nakamura H., Asashima M.
  • Journal Title: Dev.Growth Differ. Volume: 53 Issue: 1 Pages: 37-47
  • DOI: 10.1111/j.1440-169x.2010.01220.x
  • NAID: 10027899242
  • Peer Reviewed
• [Journal Article] Glycome diagnosis of human induced pluripotent stem cells using lectin microarray (2011)
  • Author(s): Tateno H., Toyoda M., Saito S., Onuma Y., Ito Y., Hiemori K., Fukumura M., Nakasu A., Nakanishi M., Ohnuma K., Akutsu H., Umezawa A., Horimoto K., Hirabayashi J., Asashima M.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 20345-20363
  • Peer Reviewed
• [Journal Article] Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb (2011)
  • Author(s): Kuwabara T., Kagalwala M.N., Onuma Y., Ito Y., Warashina M., Terashima K., Sanosaka T., Nakashima K., Gage F.H., Asashima, M
  • Journal Title: EMBO Mol.Med. Volume: 3 Issue: 12 Pages: 742-754
  • DOI: 10.1002/emmm.201100177
  • Peer Reviewed
• [Journal Article] 発生学から見た再生医療 (2011)
  • Author(s): 浅島誠、伊藤弓弦、栗崎晃
  • Journal Title: 再生医療 Volume: 10 Pages: 17-33
• [Journal Article] Novel cell surface genes expressed in the stomach primordium during gastrointestinal morphogenesis of mouse embryos (2011)
  • Author(s): Noguchi TA, Ishimine H, Nakajima Y, Watanabe-Susaki K, Shigeta N, Yamakawa N, Wang PC, Asashima M, Kurisaki A
  • Journal Title: Gene Expr Patterns Volume: (掲載確定) Issue: 3-4 Pages: 154-163
  • DOI: 10.1016/j.gep.2012.01.001
  • Peer Reviewed
• [Journal Article] Chemokine ligand Xenopus CXCLC (XCXCLC) regulates cell movements during early morphogenesis (2011)
  • Author(s): Goto T, Asashima M
  • Journal Title: Dev Growth Differ. Volume: 53 Issue: 9 Pages: 971-981
  • DOI: 10.1111/j.1440-169x.2011.01304.x
  • NAID: 10030404107
  • Peer Reviewed
• [Journal Article] The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol metabolizing enzyme (2011)
  • Author(s): T.Yoshiyama-Yanagawa, S.Enya, Y.Shimada-Niwa, S.Yaguchi, Y.Haramoto, T.Matsuya, K.Shiomi, Y.Sasakura, S.Takahashi, M.Asashima, H.Kataoka, R.Niwa
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 29 Pages: 25756-25762
  • DOI: 10.1074/jbc.m111.244384
  • Peer Reviewed
• [Journal Article] Development of Ca(2)(+)signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation. (2011)
  • Author(s): Takano K, Obata S, Komazaki S, Masumoto M, Oinuma T, Ito Y, Ariizumi T, Nakamura H, AsashimaM.
  • Journal Title: Dev.Growth Differ. Volume: 53 Pages: 37-47
  • NAID: 10027899242
  • Peer Reviewed
• [Journal Article] Translocon-associated protein subunit Trap-γ/Ssr3 is required for vascular networkformation in the mouse placenta. (2011)
  • Author(s): Yamaguchi YL, Tanaka SS, Oshima N, Kiyonari H, Asashima M, Nishinakamura R.
  • Journal Title: Dev Dyn. Volume: 240 Pages: 394-403
  • Peer Reviewed
• [Journal Article] Rapamycin treatment causes developmental delay, pigmentation defects, and gastrointestinal malformation on Xenopus embryogenesis. (2011)
  • Author(s): Moriyama Y, Ohata Y, Mori S, Matsukawa S, Michiue T, Asashima M, Kuroda H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 404 Pages: 974-978
  • Peer Reviewed
• [Journal Article] Development of defective and persistent sendai virus vector : aunique genedelivery/expression system ideal for cell reprogramming. (2011)
  • Author(s): Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, YamaguchiT, Nakanishi M.
  • Journal Title: JBiol Chem. Volume: 286 Pages: 4760-4771
  • Peer Reviewed
• [Journal Article] The transcriptional coactivators Yap and TAZ are expressed during early Xenopus development (2011)
  • Author(s): Nejigane S, Haramoto Y, Okuno M, Takahashi S, Asashima M.
  • Journal Title: Int J Dev Biol. Volume: 55 Pages: 121-126
  • Peer Reviewed
• [Journal Article] Xenopus furry contributes to release of microRNA gene silencing (2010)
  • Author(s): Goto T, Fukui A, Shibuya H, Keller R, Asashima M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 19344-19349
  • Peer Reviewed
• [Journal Article] TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner (2010)
  • Author(s): Seki Y, Kurisaki A, Watanabe-Susaki K, Nakajima Y, Nakanishi M, Arai Y, Shiota K, Sugino H, Asashima M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 10926-10931
  • Peer Reviewed
• [Journal Article] Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder-and Serum-Free Defined Conditions (2010)
  • Author(s): Hayashi Y, Chan T-C, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Kusuda Furue M, Michiue T, Ohnuma K, Nakauchi H, Asashima M
  • Journal Title: Plos One Volume: 5 Pages: 14099-14099
  • Peer Reviewed
• [Journal Article] Intensely Fluorescent Azobenzenes : Synthesis, Crystal Structures, Effects of Substituents, and Application to Fluorescent Vital Stain. (2010)
  • Author(s): Yoshino, J., Furuta, A., Kambe, T., Itoi, H., Kano, N., Kawashima, T., Ito, Y., Asashima, M.
  • Journal Title: Chem.Eur.J. Volume: 16 Pages: 5026-5035
  • Peer Reviewed
• [Journal Article] Cloning of noggin gene from hydra and analysis of its functional conservation using Xenopus laevis embryos. (2010)
  • Author(s): Chandramore, K., Ito, Y., Takahashi, S., Asashima, M., Gbaskadb, S.
  • Journal Title: Evol.Dev. Volume: 12 Pages: 267-274
  • Peer Reviewed
• [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation. (2010)
  • Author(s): Yamagishi, M., Ito, Y., Ariizumi, T., Komazaki, S., Danno, H., Michiue, T., Asashima, M.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 665-675
  • NAID: 10027566245
  • Peer Reviewed
• [Journal Article] 「DomeGene」宇宙環境が両生類培養細胞に及ぼす影響 (2010)
  • Author(s): 浅島誠、中島由郎、矢野幸子、内田智子、嶋津徹、伊藤弓弦
  • Journal Title: 生物工学 Volume: 88 Pages: 285-287
  • NAID: 110007658221
• [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development. (2010)
  • Author(s): Suzuki K, Takahashi S, Haramoto Y, Onuma Y, Nagamine K, Okabayashi K, Hashizume K, Iwanaka T, Asashima M.
  • Journal Title: Int J Dev Biol. Volume: 54 Pages: 609-615
  • Peer Reviewed
• [Journal Article] Pdxl-transfected adipose tissue-derived stem cells differentiate into insulin-producing cellsin vivo and reduce hyperglycemia in diabetic mice. (2010)
  • Author(s): Kapyama H, Hamazaki TS, TokuharaM, Masui S, Okabayashi K, Ohnuma K, Yabe S, Yasuda K, Ishiura S, Okochi H, Asashima M.
  • Journal Title: Int J Dev Biol. Volume: 54 Pages: 699-705
  • Peer Reviewed
• [Journal Article] BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin. (2010)
  • Author(s): Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.
  • Journal Title: In Vitro Cell Dev Biol Anim. Volume: 46 Pages: 416-430
  • Peer Reviewed
• [Journal Article] Creating frog heart as an organ : in vitro-induced heart functions as a circulatory organ in-VIVO. (2010)
  • Author(s): Kinoshita, M., Ariizumi, T., Yuasa, S., Miyoshi, S., Komazaki, S., Fukuda, K., Asashima, M.
  • Journal Title: Int J Dev Biol. Volume: 54 Pages: 851-856
  • Peer Reviewed
• [Journal Article] Induction of neural crest cells from mouse embryonic stem cells in a serum-free monolayer culture. (2010)
  • Author(s): Aihara Y, Hayashi Y, Hirata M, ArikiN, Shibata S, Nagoshi N, NakanishiM, Ohnuma K, Warashina M, Michiue T, Uchiyama H, Okano H, Asashima M, Kusuda Furue M.
  • Journal Title: Int J Dev Biol. Volume: 54 Pages: 1287-1294
  • Peer Reviewed
• [Journal Article] An In Vitro Reconstitution System for the Assessment of Chromatin Protein Fluidity duringXenopus Development. (2010)
  • Author(s): Aoki R, Inui M, Hayashi Y, SedoharaA, Okabayashi K, Ohnuma K, Murata M, Asashima M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 400 Pages: 200-206
  • Peer Reviewed
• [Journal Article] Efficiently Differentiating Vascular Endothelial Cells from Adipose Tissue DerivedMesenchymal Stem Cells in Serum-Free Culture. (2010)
  • Author(s): Konno M, Hamazaki TS, Fukuda S, Tokuhara M, Uchiyama H, Okazawa H, Okochi H, Asashima M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 400 Pages: 461-465
  • Peer Reviewed
• [Journal Article] TIFIbeta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. (2010)
  • Author(s): Seki Y, Kurisaki A, Watanabe-Susaki K, Nakajima Y, Nakanishi M, Arai Y, Shiota K, Sugino H, Asashima M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 10926-10931
  • Peer Reviewed
• [Journal Article] Inhibitory Smad proteins promote the differentiation of mouse embryonic stem cells into ependymal-like ciliated cells. (2010)
  • Author(s): Nishimura Y, Kurisaki A, Nakanishi M, Ohnuma K, Ninomiya N, KomaZaki S, Ishiura S, Asashima M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 401 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Xenopus furry contributes to release of microRNA gene silencing. (2010)
  • Author(s): Goto T, Fukui A, Shibuya H, Kelley R, Asashima M
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 19344-19349
  • Peer Reviewed
• [Journal Article] Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder-and Serum-Free Defined Conditions. (2010)
  • Author(s): Hayashi Y, Chan T-C, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, i Eto K, Kusuda Furue M, Michiue T, Ohnuma K, Nakauchi H, Asashima M
  • Journal Title: PlosOne Volume: 5
  • Peer Reviewed
• [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development. (2010)
  • Author(s): Suzuki K, Takahashi S, Haramoto Y, Onuma Y, Nagamine K, Okabayashi K, Hashizume K, Iwanaka T
  • Journal Title: Int J Dev Biol. 54(4) Pages: 609-615
  • Peer Reviewed
• [Journal Article] Pdx1-transfected adipose tissue-derived stem cells differentiate into insulin-producing cells in vivo and reduce hyperglycemia in diabetic mice. (2010)
  • Author(s): Kajiyama H, Hamazaki TS, Tokuhara M, Masui S, Okabayashi K, Ohnuma K, Yabe S, Yasuda K
  • Journal Title: Int J Dev Biol. 54(4) Pages: 699-670
  • Peer Reviewed
• [Journal Article] Zygotic VegT is required for Xenopus paraxial mesoderm formation and is regulated by Nodal signaling and Eomesodermin. (2010)
  • Author(s): Fukuda M, Takahashi S, Haramoto Y, Onuma Y, Kim YJ, Yeo CY, Ishiura S, Asashima M
  • Journal Title: Int J Dev Biol. 54(1) Pages: 81-92
  • Peer Reviewed
• [Journal Article] BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin. (2010)
  • Author(s): Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aibara Y
  • Journal Title: n Vitro Cell Dev Biol Anim. (印刷中)
  • Peer Reviewed
• [Journal Article] Creating frog heart as an organ : in vitro-induced heart functions as a circulatory organ in vivo. (2010)
  • Author(s): Kinoshita M, Ariizumi T, Yuasa S, Miyoshi S, Komazaki S, Fukuda K, Asashima M.
  • Journal Title: Int J Dev Biol. (印刷中)
  • Peer Reviewed
• [Journal Article] In vitro organogenesis from undifferentiated cells in Xenopus (2009)
  • Author(s): Asashima M, Ito Y, Chan T, Michiue T, Nakanishi M, Suzuki K, Hitachi K, Okabayashi K, Kondow A, Ariizumi T
  • Journal Title: Dev Dyn Volume: 238 Pages: 1309-1320
  • Peer Reviewed
• [Journal Article] Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium (2009)
  • Author(s): Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M
  • Journal Title: FASEB J Volume: 23 Pages: 114-122
  • Peer Reviewed
• [Journal Article] Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis (2009)
  • Author(s): Kuwabara T, Hsieh J, Muotri A, Yeo G, Warashina M, Lie DC, Moore L, Nakashima K, Asashima M, Gage FH
  • Journal Title: Nat Neurosci Volume: 12 Pages: 1097-1105
  • Peer Reviewed
• [Journal Article] The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development (2009)
  • Author(s): Takada H, Kawana T, Ito Y, Kikuno RF, Mamada H, Araki T, Koga H, Asashima M, Taira M
  • Journal Title: Development Volume: 136 Pages: 2413-2422
  • Peer Reviewed
• [Journal Article] The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors. (2009)
  • Author(s): Hitachi K, Danno H, Tazumi S, Aihara Y, Uchiyama H, Okabayashi K, Kondow A, Asashima M
  • Journal Title: Int J Dev Biol. 54(4) Pages: 631-639
  • Peer Reviewed
• [Journal Article] Bestrophin genes are expressed in Xenopus development. (2009)
  • Author(s): Onuma Y, Haramoto Y, Nejigane S, Takahashi S, Asashima M.
  • Journal Title: Biochem Biophys Res Commun. 384(3) Pages: 290-295
  • Peer Reviewed
• [Journal Article] Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix-assisted laser desorption/ionization-based proteomic analysis. (2009)
  • Author(s): Intoh A, Kurisaki A, Fukuda H, Asashima M.
  • Journal Title: Biomed Chromatogr. 23(6) Pages: 607-614
  • Peer Reviewed
• [Journal Article] In vitro organogenesis from undifferentiated cells in Xenopus. (2009)
  • Author(s): Asashima M, Ito Y, Chan T, Michiue T, Nakanishi M, Suzuki K, Hitachi K, Okabayashi K, Kondow A
  • Journal Title: Dev Dyn. 238(6) Pages: 1309-1320
  • Peer Reviewed
• [Journal Article] Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis. (2009)
  • Author(s): Yamada S, Onishi M, Fujinawa R, Tadokoro Y, Okabayashi K, Asashima M, Sugahara K
  • Journal Title: Glycobiology 19(5) Pages: 488-498
  • NAID: 120002087034
  • Peer Reviewed
• [Journal Article] Decreased expression of CXXC4 promotes a malignant phenotype in renal cell carcinoma by activating Wnt signaling. (2009)
  • Author(s): Kojima T, Shimazui T, Hinotsu S, Joraku A, Oikawa T, Kawai K, Horie R, Suzuki H, Nagashima R
  • Journal Title: Oncogene 28(2) Pages: 297-305
  • Peer Reviewed
• [Journal Article] Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. (2009)
  • Author(s): Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Eurue M, Asashima M
  • Journal Title: FASEB J. 23(1) Pages: 114-122
  • Peer Reviewed
• [Journal Article] Proteomic analysis of membrane proteins expressed specifically in pluripotent murine embryonic stem cells. (2009)
  • Author(s): Intoh A, Kurisaki A, Yamanaka Y, Hirano H, Fukuda H, Sugino H, Asashima M
  • Journal Title: Proteomics 9(1) Pages: 126-137
  • Peer Reviewed
• [Journal Article] Directional migration of neuronal PC12 cells in a ratchet wheel shaped microchamber (2009)
  • Author(s): Ohnuma, K., Toyota, T., Ariizumi, T., Sugawara, T., Asashima, M.
  • Journal Title: Journal of Bioscience and Bioengineering 108(1) Pages: 76-83
  • NAID: 110007359687
  • Peer Reviewed
• [Journal Article] Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. (2009)
  • Author(s): Kuwabara T, Hsieh J, Muotri A, Yeo G, Warashina M, Lie DC, Moore L, Nakashima K, Asashima M, Gage
  • Journal Title: Nat Neurosci. 12(9) Pages: 1097-1105
  • Peer Reviewed
• [Journal Article] The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development. (2009)
  • Author(s): Takada H, Kawana T, Ito Y, Kikuno RF, Mamada H, Araki T, Koga H, Asashima M, Taira M
  • Journal Title: Development 136(14) Pages: 2413-2422
  • Peer Reviewed
• [Journal Article] Isolation and differentiation of Xenopus animal cap cells. (2009)
  • Author(s): Ariizumi T, Takahashi S, Chan TC, Ito Y, Michiue T, Asashima M.
  • Journal Title: Curr Protoc Stem Cell Biol. Chapter 1
• [Presentation] 幹細胞評価基盤技術開発に向けたアプローチ (2012)
  • Author(s): 伊藤弓弦
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2012-01-31
• [Presentation] 持統発現型RNAベクターを用いたヒトiPS細胞樹立法の確立 (2012)
  • Author(s): 大高真奈美、佐野将之、西村健、高安聡子、小沼泰子、中島由郎、伊藤弓弦、池原譲、浅島誠、中西真人
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2012-01-31
• [Presentation] 心臓形成におけるMEF2cスプライシングバリアントの機能解析 (2012)
  • Author(s): 野曽原志帆、原本悦和、小沼泰子、伊藤弓弦、王碧昭
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2012-01-31
• [Presentation] 初期心臓形成に関与するヒアルロン酸経路の解析 (2012)
  • Author(s): 岩本麻未、伊藤弓弦、王碧昭
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2012-01-31
• [Presentation] 初期心臓形成に関与するヒアルロン酸シグナル経路の解析 (2012)
  • Author(s): 岩本麻未、伊藤弓弦、王碧昭
  • Organizer: テクノロジーショーケースinつくば2012
  • Place of Presentation: つくば
  • Year and Date: 2012-01-13
• [Presentation] Isolation and characterization of sexually dimorphic genes during early gonadogenesis in Xenopus laevis (2011)
  • Author(s): Shuji Mawaribuchi, Nozomi Ikeda, Kazuko Fujitani, Asako Otomo, Kei Tamura, Mikako Wada, Yosuke Kamata, Yuzuru Ito, Yasuko Onuma, Mihcihiko Ito, Nobuhiko Takamatsu
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] ネッタイツメガエルの研究基盤整備 (2011)
  • Author(s): 矢尾板芳郎、浅島誠、高瀬稔、高橋秀治、伊藤弓弦
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] ヒトiPS細胞を用いた無血清・無フィーダー培養による心筋誘導系の確立 (2011)
  • Author(s): 小池怜、伊藤弓弦、吉満亮介、丸山篤史、大沼清
  • Organizer: 第33回日本バイオマテリアル学会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-21
• [Presentation] ツメガエル胚のアニマルキャップから分化誘導した膵臓の血糖調節能 (2011)
  • Author(s): 有泉高史、セン徳川、高橋秀治、浅島誠
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエル2次胚形成における遺伝子発現 (2011)
  • Author(s): 佐藤英二、小沼泰子、三浦直行、伊藤弓弦
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] ツメガエル胚のアニマルキャップから分化誘導した膵臓の血糖調節能 (2011)
  • Author(s): 有泉高史、セン徳川、高橋秀治、浅島誠
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 大雪クリスタルホール(旭川)
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエル2次胚形成における遺伝子発現について (2011)
  • Author(s): 佐藤英二、小沼泰子、三浦直行、伊藤弓弦
  • Organizer: 第44回日本発生生物学会(APDBN共催)
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-18
• [Presentation] Approaches to Single-Cell Analysis (2011)
  • Author(s): Asashima, M.
  • Organizer: The 5th International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: Tokyo
  • Year and Date: 2011-03-03
• [Presentation] iPS細胞樹立に最適な形に進化させた持続発現型RNAベクターの開発 (2011)
  • Author(s): 西村健、大高真奈美、高安聡子、小林俊寛、小沼泰子、伊藤弓弦、大津真、中内啓光、浅島誠、中西真人
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2011-03-01
• [Presentation] 高密度レクチンアレイの開発と細胞グライコミクスへの応用 (2010)
  • Author(s): 舘野浩章、豊田雅士、小沼泰子、伊藤弓弦、斉藤秀、阿久津英憲、梅澤明弘、堀本勝久、浅島誠、平林淳
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-10
• [Presentation] ChIPシーケンス解析で明らかとなった原腸胚オーガナイザーの遺伝子制御ネットワークにおけるLim1/Lhx1とOtx2の多機能性 (2010)
  • Author(s): 安岡有理、須藤則広、鈴木穣、高橋秀治、原本悦和、浅島誠、菅野純夫、平良眞規
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 陣戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-07
• [Presentation] 新規BMP阻害因子Dullardは、骨芽細胞においてBMP受容体及びSmad1の下流でBMP応答性の転写を抑制する (2010)
  • Author(s): 早田匡芳、江面陽一、西中村隆一、浅島誠、野田政樹
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 陣戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-07
• [Presentation] 水溶性官能基を有する蛍光性アゾベンゼンの合成と蛍光生体染色への応用 (2010)
  • Author(s): 糸井裕亮、吉野惇郎、神戸徹也、狩野直和、川島隆幸、伊藤弓弦、浅島誠
  • Organizer: 第37回有機典型元素化学討論会
  • Place of Presentation: 室蘭市民会館ホール(北海道)
  • Year and Date: 2010-11-25
• [Presentation] 再生医療の現状と今後 (2010)
  • Author(s): 浅島誠
  • Organizer: 平成22年度裁判基盤研究会「生命科学」
  • Place of Presentation: 司法研修所(埼玉)
  • Year and Date: 2010-10-27
• [Presentation] Challenge the establishment of new fundamental technology in the regenerative medicine (2010)
  • Author(s): Ito, Y
  • Organizer: 2nd Symposium on Systems and Synthetic Biology(TriSys)
  • Place of Presentation: Tokyo
  • Year and Date: 2010-10-04
• [Presentation] Challenge the establishment of new fundamental technology in the regenerative medicine. (2010)
  • Author(s): Ito, Y.
  • Organizer: 2nd Symposium on Systems and Synthetic Biology(TriSys)
  • Place of Presentation: 産総研臨海(東京)
  • Year and Date: 2010-10-04
• [Presentation] Organogenesis and control of differentiation in vertebrate development (2010)
  • Author(s): Asashima, M
  • Organizer: International Conference on Functional Genomics
  • Place of Presentation: India
  • Year and Date: 2010-10-02
• [Presentation] Organogenesis and control of differentiation in vertebrate development. (2010)
  • Author(s): Makoto Asashima
  • Organizer: International Conference on Functional Genomics
  • Place of Presentation: Banaras Hindu University(lndia)
  • Year and Date: 2010-10-02
• [Presentation] Dome gene experiment of space flight done in ISS (2010)
  • Author(s): Makoto Asashima
  • Organizer: 7th Korea-Japan Joint Seminar on Space Environment Utilization Research
  • Place of Presentation: Forest Hongo(東京)
  • Year and Date: 2010-09-26
• [Presentation] 脊椎動物の未分化細胞からの中胚葉誘導と器官形成 (2010)
  • Author(s): 浅島誠
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] 脊椎動物の未分化細胞からの中胚葉誘導と器官形成- (2010)
  • Author(s): 浅島誠
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東大駒場キャンパス(東京)
  • Year and Date: 2010-09-23
• [Presentation] 脊椎動物原腸胚オーガナイザーにおける転写制御ネットワークとその進化 (2010)
  • Author(s): 安岡有理、須藤則広、高橋秀治、鈴木穣、丹藤由希子、窪川かおる、菅野純夫、浅島誠、平良眞規
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東大駒場キャンパス(東京)
  • Year and Date: 2010-09-23
• [Presentation] Promoting best practices for scientists and post-doctoral fellows (2010)
  • Author(s): Makoto Asashima
  • Organizer: 2nd World Conference on Research Integrity
  • Place of Presentation: Pan Pacific Hotel (Singapore)
  • Year and Date: 2010-07-23
• [Presentation] 脊椎動物の未分化細胞からの器官形成と分化制御 (2010)
  • Author(s): 浅島誠
  • Organizer: iPS・ES・体性幹細胞Forum2010一基礎研究から臨床応用・創薬研究へ-
  • Place of Presentation: 秋葉原UDX Gallaery(東京)
  • Year and Date: 2010-06-29
• [Presentation] Life science experiments in ISS (2010)
  • Author(s): Makoto Asashima
  • Organizer: Symposium on Scientific Utilization of JEM Kibo on International Space Station(ISS)
  • Place of Presentation: Suzuki Umetaro HallWako, RIKEN(埼玉)
  • Year and Date: 2010-04-28
• [Presentation] 脊椎動物の未分化細胞からの臓器形成と分化制御 (2010)
  • Author(s): 浅島誠
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-27
• [Presentation] 脊椎動物の未分化細胞からの臓器形成と分化制御 (2010)
  • Author(s): 浅島誠
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2010-04-27
• [Presentation] 脊椎動物の未分化細胞からの臓器形成と分化制御 (2010)
  • Author(s): 浅島誠
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2010-03-18
• [Presentation] Organogenesis and control of differentiation in vertebrates (2010)
  • Author(s): Asashima, M
  • Organizer: Japan-Israel Workshop on Stem Cells
  • Place of Presentation: Jerusalem
  • Year and Date: 2010-02-25
• [Presentation] Organogenesis and control of differentiation in vertebrates (2010)
  • Author(s): Asahima, M.
  • Organizer: Japan-Israel Workshop on Stem Cells
  • Place of Presentation: Hebrew University, Jerusalem
  • Year and Date: 2010-02-25
• [Presentation] 脊椎動物の未分化細胞からの器官形成と分子機序 (2010)
  • Author(s): 浅島誠
  • Organizer: 発生工学・疾患モデル研究会第79回定例会
  • Place of Presentation: 国立がんセンター研究所1Fセミナールーム
  • Year and Date: 2010-01-23
• [Presentation] 脊椎動物の未分化細胞からの臓器形成と分化制御 (2009)
  • Author(s): 浅島誠
  • Organizer: 第28回日本運動器移植・再生医学研究会
  • Place of Presentation: 富士ソフトアキバプラザ
  • Year and Date: 2009-11-21
• [Presentation] 細胞の分化をはかる (2009)
  • Author(s): 浅島誠
  • Organizer: 第6回コンビナトリアル・バイオエンジニアリング会議
  • Place of Presentation: 千里阪急ホテル2F樹林の間
  • Year and Date: 2009-11-13
• [Presentation] Complex system and regulation in early animal development (2009)
  • Author(s): Asasahima, M
  • Organizer: International Symposium on Complex Systems Biology
  • Place of Presentation: Tokyo
  • Year and Date: 2009-10-01
• [Presentation] Complex system and regulation in early animal development (2009)
  • Author(s): Asasahima, M.
  • Organizer: International Symposium on Complex Systems Biology
  • Place of Presentation: Tetsumon Memorial Hall, The University of Tokyo
  • Year and Date: 2009-10-01
• [Presentation] イモリ胚の原腸陥入における内胚葉細胞の運動と細胞内カルシウムイオン動員機構 (2009)
  • Author(s): 増本三香、小畑秀一、高野和敬、駒崎伸二、生沼勉、浅島誠
  • Organizer: 日本動物学会第80回静岡大会
  • Place of Presentation: 静岡県コンベンションアーツセンターグランシップ
  • Year and Date: 2009-09-19
• [Presentation] イモリ初期原腸胚から単離した予定内胚葉細胞にみられる細胞伸長及び細胞運動のしくみ (2009)
  • Author(s): 小畑秀一、増本三香、高野和敬、駒崎伸二、生沼勉、浅島誠
  • Organizer: 日本動物学会第80回静岡大会
  • Place of Presentation: 静岡県コンベンションアーツセンターグランシップ
  • Year and Date: 2009-09-19
• [Presentation] アフリカツメガエル初期胚の表層と内層の反応性の差違 (2009)
  • Author(s): 高橋秀治、袮次金進、原本悦和、有泉高史、浅島誠
  • Organizer: 日本動物学会第80回静岡大会
  • Place of Presentation: 静岡県コンベンションアーツセンターグランシップ
  • Year and Date: 2009-09-19
• [Presentation] In vitro organogenesis in vertebrate development (2009)
  • Author(s): Asasahima, M
  • Organizer: 16th International Society of Developmental Biologists Congress 2009
  • Place of Presentation: Edinburgh
  • Year and Date: 2009-09-08
• [Presentation] In vitro organogenesis in vertebrate development (2009)
  • Author(s): Asasahima, M.
  • Organizer: 16th International Society of Developmental Biologists Congress 2009
  • Place of Presentation: Edinburgh, Scotland, UK
  • Year and Date: 2009-09-08
• [Presentation] Directed induction of primitive streak from embryonic stem cells cultured in a chemically defined medium. (2009)
  • Author(s): Nakanishi, M., Kurisaki, A., Hayashi, Y., Warashina, M., Ishiura, S, Eurue MK, Asashima M
  • Organizer: The 9th World Congress of Inflammation
  • Place of Presentation: Keio Plaza Hotel
  • Year and Date: 2009-07-08
• [Presentation] Identification of cell surface markers expressed in pluripotent embryonic stem cells (2009)
  • Author(s): Intoh, A., Kurisaki, A., Sugino, H., Asashima, M.
  • Organizer: The 9th World Congress of Inflammation
  • Place of Presentation: Keio Plaza Hotel
  • Year and Date: 2009-07-08
• [Presentation] ES細胞・iPS細胞・組織幹細胞研究の現状と再生医療実現に向けた課題 (2009)
  • Author(s): 浅島誠
  • Organizer: 第2回羊膜再生医療研究会
  • Place of Presentation: 北里大学医療衛生学部
  • Year and Date: 2009-05-23
• [Book] Mechanosensing Biology (2010)
  • Author(s): Asashima M, Michiue T, Ohnuma K, Nakajima Y, Ito Y
  • Publisher: Springer
• [Book] 生物学辞典(第1版) (2010)
  • Author(s): 浅島誠
  • Total Pages: 1634
  • Publisher: 東京化学同人
• [Book] Chapter 3 Mechanobiology During Vertebrate Organ Development, Mechanosensing Biology (2010)
  • Author(s): Makoto Asashima, Tatsuo Michiue, Kiyoshi Ohnuma, Yoshiro Nakapma, Yuzuru lto
  • Publisher: Springer
• [Book] 生物学辞典(第一版) (2010)
  • Author(s): 浅島誠
  • Total Pages: 1634
  • Publisher: 東京化学同人
• [Book] 研究をささえるモデル生物カエルがわかる (2009)
  • Author(s): 浅島誠、道上達男、伊藤弓弦
  • Total Pages: 11
  • Publisher: 化学同人
• [Book] 再生医療 (2009)
  • Author(s): 岡林浩嗣、大沼清、浅島誠
  • Total Pages: 6
  • Publisher: メディカルレビュー社
• [Book] 研究をささえるモデル生物 カエルがわかる (2009)
  • Author(s): 浅島誠、道上達男、伊藤弓弦
  • Total Pages: 11
  • Publisher: 化学同人