Systematic research of stable isotopic fractionation and equilibrate effects during metabolisms of extremophiles

• Project/Area Number: 21244077
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TAKAI Ken 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, プログラムディレクター (80359166)
• Co-Investigator(Kenkyū-buntansha): UENO Yu-ichiro 東京工業大学, 大学院・理工学研究科, 准教授 (90422542)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥48,880,000 (Direct Cost: ¥37,600,000、Indirect Cost: ¥11,280,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥45,240,000 (Direct Cost: ¥34,800,000、Indirect Cost: ¥10,440,000)
• Keywords: 地球史 / 微生物活動 / 水素代謝 / 硫黄代謝 / 質量非依存分別

Research Abstract

We completed the experiments for effect of H2-consuming and. producing metabolisms on stable hydrogen isotopic equilibrium, effect of S0-reducing metabolisms on multiple sulfur isotopic equilibrium and fractionation and effect of sulfur-disproportionating metabolisms on multiple sulfur isotopic fractionation. All the data have provided very new insights into understanding the systematics of stable isotopic fractionation and equilibrate effects during metabolisms of extremophiles. Several research papers are now under preparation.

Research Products

• [Journal Article] Seasonal change in microbial sulfur cycling in monomictic Lake Fukami-ike, Japan. (2012)
  • Author(s): Nakagawa, M., Ueno, Y., Hattori, S., Umemura, M., Yagi, A., Takai, K., Koba, K., Sasaki, Y., Makabe, A., and Yoshida, N.
  • Journal Title: Limnol. Oceanography Volume: (in press)
  • Peer Reviewed
• [Journal Article] A deeply branching thermophilic bacterium with an ancient acetyl-CoA pathway dominates a subsurface ecosystem (2012)
  • Author(s): Hideto Takami
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: e30559-e30559
  • DOI: 10.1371/journal.pone.0030559
  • Peer Reviewed
• [Journal Article] Domain-level identification and quantification of relative prokaryotic cell abundance in microbial communities by Micro-FTIR spectroscopy (2012)
  • Author(s): Motoko Igisu
  • Journal Title: Environmental Microbiology Report Volume: 4 Issue: 1 Pages: 42-49
  • DOI: 10.1111/j.1758-2229.2011.00277.x
  • Peer Reviewed
• [Journal Article] Sulfur metabolisms in epsilon- and gamma-Proteobacteria in deep-sea hydrothermal fields (2011)
  • Author(s): Masahiro Yamamoto
  • Journal Title: Frontiers in Microbiology Volume: 2 Pages: 192-192
  • DOI: 10.3389/fmicb.2011.00192
  • Peer Reviewed
• [Journal Article] Genome sequence of a mesophilic hydrogenotrophic methanogen Methanocella paludicola, the first cultivated representative of the order Methanocellales (2011)
  • Author(s): Sanae Sakai
  • Journal Title: PLoS ONE Volume: 6 Issue: 7 Pages: e22898-e22898
  • DOI: 10.1371/journal.pone.0022898
  • Peer Reviewed
• [Journal Article] ヒ素をリンの代わりにする細菌 (2011)
  • Author(s): 高井研
  • Journal Title: 日経サイエンス Volume: 41 Pages: 24-26
• [Journal Article] 地球生命誕生の物語 (2011)
  • Author(s): 高井研
  • Journal Title: milsil Volume: 2 Pages: 22-25
• [Journal Article] Isotopic variation of molecular hydrogen in 20o-375oC hydrothermal fluids as detected by a new analytical method (2010)
  • Author(s): Kawagucci, S., Toki, T., Ishibashi, J., Takai, K., Ito, M., Oomori, T., and Gamo, T.
  • Journal Title: J. Geophys. Res. Volume: 115
  • Peer Reviewed
• [Journal Article] Molecular characterization of inorganic sulfur-compounds metabolisms in a deep-sea epsilon-proteobacterium Sulfurovum sp. (2010)
  • Author(s): Yamamoto, M., Nakagawa, S., Shimamura, S., Takai, K., and Horikoshi, K.
  • Journal Title: NBC37-1.Environ. Volume: 12 Pages: 1144-1153
  • Peer Reviewed
• [Journal Article] Molecular characterization of inorganic sulfur-compounds metabolisms in a deep-sea epsilon-proteobacterium Sulfurovum sp.NBC37-1. (2010)
  • Author(s): Yamamoto M.
  • Journal Title: Environmental Microbiology Volume: 12 Pages: 1144-1153
  • Peer Reviewed
• [Journal Article] Isotopic variation of molecular hydrogen in 20°-375°C hydrothermal fluids as detected by a new analytical method. (2010)
  • Author(s): Kawagucci S
  • Journal Title: Journal of Geophysical Research Volume: 115
  • Peer Reviewed
• [Journal Article] Highly alkaline, high-temperature hydrothermal fluids in the early Archcan ocean. (2010)
  • Author(s): Shibuya T
  • Journal Title: Precambrian Research Volume: 182 Pages: 230-238
  • Peer Reviewed
• [Journal Article] 地球化学と微生物学から迫る非生命圏と生命圏の境界 (2010)
  • Author(s): 高井研
  • Journal Title: 地球化学 Volume: 44 Pages: 103-114
  • NAID: 110009327689
  • Peer Reviewed
• [Journal Article] Molecular characterization of inorganic sulfur-compounds metabolisms in a deep-sea epsilon-proteobacterium Sulfurovum sp.NBC37-1 (2010)
  • Author(s): Yamamoto M
  • Journal Title: Environmental Microbiology
  • Peer Reviewed
• [Journal Article] Biological fractionations of quadruple sulfur isotopes in a stratified lake (2009)
  • Author(s): Nakagawa M
  • Journal Title: Proceedings of The 4th International Symposium on Isotopomers, Tokyo Pages: 26-30
• [Presentation] Hydrogen isotope systematics of H2-H2O-CH4 during hydrogenotrophic methanogenesis (2011)
  • Author(s): Kobayashi, M., Kawagucci, S., Hattori, S., Yamada, K., Ueno, Y., Takai, K., Yoshida, N.
  • Organizer: AGU Fall Meeting 2011
  • Place of Presentation: Moscone Center, San Francisco, California
  • Year and Date: 2011-12-10
• [Presentation] メタンの4つの水素はすべて水に由来するのか:H2-H2O-CH4の水素同位体システマティクスから考える水素資化メタン生成代謝におけるメタンの水素同位体比決定機構 (2011)
  • Author(s): 川口慎介,小林真理子,服部祥平,山田桂太,上野雄一郎,高井研,吉田尚弘
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学,京都
  • Year and Date: 2011-10-08
• [Presentation] メタンの4つの水素はすべて水に由来するのか:H2-H2O-CH4の水素同位体システマティクスから考える水素資化メタン生成代謝におけるメタンの水素同位体比決定機構 (2011)
  • Author(s): 川口慎介
  • Organizer: 日本微生物生態学会第27回大会
  • Place of Presentation: 京都大学農学部、京都
  • Year and Date: 2011-10-08
• [Presentation] 地下メタン資源の起源に関するカーナビ時代の伊能忠敬的研究 (2011)
  • Author(s): 川口慎介
  • Organizer: 日本地球化学会年会
  • Place of Presentation: 北海道大学、北海道
  • Year and Date: 2011-09-15
• [Presentation] Novel sulfur disproportionation of a deep-sea vent thermophile (2011)
  • Author(s): Takai, K.
  • Organizer: International Union of Microbiology Societies Congress 2011
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2011-09-08
• [Presentation] Exploration of limits of life and unseen microbial potentials in the Dark Ecosystem (2011)
  • Author(s): Ken Takai
  • Organizer: International Union of Microbiology Societies Congressnn 2011
  • Place of Presentation: 札幌コンベンションセンター、北海道(招待講演)
  • Year and Date: 2011-09-08
• [Presentation] H2-H2O-CH4の水素同位体システマティクスから考える微生物由来メタンの水素同位体比決定機構の解明 (2011)
  • Author(s): 小林真理子,川口慎介,服部祥平,山田桂太,上野雄一郎,高井研,吉田尚弘
  • Organizer: 2011年度日本地球化学会
  • Place of Presentation: 北海道大学,札幌
  • Year and Date: 2011-09-06
• [Presentation] Origin of methane in the Okinawa Trough hydrothermal fields : Implication for long transport of biogenic methane at subsurface geofluid system (2011)
  • Author(s): Shinsuke Kawagucci
  • Organizer: EGU General Assembly Meeting 2011
  • Place of Presentation: Vienna, Austlia
  • Year and Date: 2011-04-05
• [Presentation] 生命を創る環境を創るat 40億年前or in宇宙 (2010)
  • Author(s): 高井研
  • Organizer: 細胞を創る会研究会3.0
  • Place of Presentation: 東京大学生産技術研究所、東京(招待講演)
  • Year and Date: 2010-11-12
• [Presentation] エネルギー論から見た宇宙における生命存在条件 (2010)
  • Author(s): 高井研
  • Organizer: 第3回アストロバイオロジーワークショップ
  • Place of Presentation: 多摩永山情報教育センター、東京
  • Year and Date: 2010-10-30
• [Presentation] Novel sulfur disproportionation of a deep-sea vent thermophile (2010)
  • Author(s): Hasegawa H
  • Organizer: Goldschmidt 2010
  • Place of Presentation: Convention Center, Knoxville, Tennessee
  • Year and Date: 2010-06-13
• [Presentation] Novel sulfur disproportionation of a deep-sea vent thermophile (2010)
  • Author(s): Hasegawa, H., Yamamoto, M., Nunoura, T., Kawagucci, S., Ueno, Y., Takai, K.
  • Organizer: Goldschmidt 2010
  • Place of Presentation: Knoxville Convention Center, Tennessee
  • Year and Date: 2010-06-08
• [Presentation] 化学合成生態系におけるH2消費を安定同位体比を用いて評価する (2010)
  • Author(s): 川口慎介,高井研
  • Organizer: 地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ,千葉
  • Year and Date: 2010-05-24
• [Presentation] 新しい微生物ドメインレベル識別・定量法の可能性 (2010)
  • Author(s): 高井研
  • Organizer: 地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2010-05-22
• [Presentation] Quadruple Sulfur Isotope Analysis of Sulfur Cycle in a Stratified Lake (2009)
  • Author(s): Nakagawa M
  • Organizer: Goldschmidt2009
  • Place of Presentation: Convention Center, Davos, Switzland
  • Year and Date: 2009-06-24
• [Book] 生命はなぜ生まれたのか-地球生物の起源の謎に迫る- (2011)
  • Author(s): 高井研
  • Total Pages: 246
  • Publisher: 幻冬舎
• [Remarks]
  • URL: http://www.jamstec.go.jp/biogeos/j/xbr/sugar/
• [Remarks]
  • URL: http://www.jamstec.go.jp/less/precam/j/
• [Remarks]
  • URL: http://www.jamstec.go.jp/shigen/j/organization/ht/