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Development of novel nucleic acid materials that efficiently work in the intracellular molecular environment

Research Project

Project/Area Number 21245040
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionKonan University

Principal Investigator

SUGIMOTO Naoki  甲南大学, フロンティアサイエンス学部, 教授 (60206430)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Junji  甲南大学, フロンティアサイエンス学部, 教授 (40289012)
NAKANO Shu-ichi  甲南大学, フロンティアサイエンス学部, 准教授 (70340908)
MIYOSHI Daisuke  甲南大学, フロンティアサイエンス学部, 准教授 (50388758)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2010: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Keywords核酸 / 四重鎖構造 / 相互作用エネルギー / リボザイム / 分子クラウディング / ポリエチレングリコール / 四重らせん構造 / 熱力学的安定性 / DNAチップ / ヒストンペプチド / アプタマー / PCR / バイオセンサー / DNA / ラロメア配列 / 枝分かれ構造 / 金属イオン
Research Abstract

We studied nucleic acid interactions and reactions by using a model experimental system that mimics the intracellular molecular environment. This research provided quantitative insights into influences of the molecular crowding medium on nucleic acid folding, a ribozyme activity, and interactions with proteins and peptides. It is supposed that these data can bridge the gap between the results obtained in vitro and in cells. Based on results regarding the effects of molecular environment, we developed novel nucleic acid materials and sensing methods that efficiently work under unusual conditions such as in living cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (118 results)

All 2012 2011 2010 2009 Other

All Journal Article (41 results) (of which Peer Reviewed: 38 results) Presentation (61 results) Book (12 results) Remarks (4 results)

  • [Journal Article] Detection of a prognostic indicator in early-stage cancer using functionalized graphene-based peptide sensors.2012

    • Author(s)
      L. Feng, L. Wu, J. Wang, J. Ren, D. Miyoshi, N. Sugimoto, and X. Qu
    • Journal Title

      Adv. Mater.

      Volume: 24 Issue: 1 Pages: 125-131

    • DOI

      10.1002/adma.201103205

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe2011

    • Author(s)
      X. Wang, L. Wu, J. Ren, D. Miyoshi, N. Sugimoto, and X. Qu
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 26 Issue: 12 Pages: 4804-4809

    • DOI

      10.1016/j.bios.2011.06.012

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measurements of the binding of a large protein using a substrate density-controlled DNA chip.2011

    • Author(s)
      S. Nakano, T. Kanzaki, M. Nakano, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Anal. Chem.

      Volume: 83 Issue: 34 Pages: 6368-6372

    • DOI

      10.1021/bi200477g

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rational design of a new IMP aptamer based on a TPP riboswitch and a hypoxanthine aptamer2011

    • Author(s)
      M. Tokui, T. Yamauchi, D. Miyoshi, H. Kamiya, A. Nishimura, and N. Sugimoto
    • Journal Title

      Chem. Lett.

      Volume: 40 Issue: 11 Pages: 1313-1314

    • DOI

      10.1246/cl.2011.1313

    • NAID

      130004426103

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of protein binding on DNA G-quadruplex structures2011

    • Author(s)
      S. Nagatoishi, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Int. Rev. Biophys. Chem.

      Volume: 2 Pages: 129-134

    • URL

      http://www.praiseworthyprize.com/IREBIC-latest/IREBIC_vol_2_n_4.html#Effects_of_protein_binding_on_DNA_Gquadruplex_structures

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene regulation system with an artificial RNA switch operating in human cell2011

    • Author(s)
      T. Endoh and N. Sugimoto
    • Journal Title

      Chem Bio Chem

      Volume: 12 Issue: 8 Pages: 1174-1178

    • DOI

      10.1002/cbic.201100093

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide2011

    • Author(s)
      S. Pramanik, K. Nakamura, K. Usui, S. Nakano, S. Saxena, J. Matsui, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Chem. Commun.

      Volume: 47 Issue: 10 Pages: 2790-2792

    • DOI

      10.1039/c0cc05776b

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of protein binding on DNA G-quadruplex structures2011

    • Author(s)
      S.Nagatoishi, D.Miyoshi, N.Sugimoto
    • Journal Title

      Int.Rev.Biophys.Cbem.

      Volume: 2 Pages: 129-134

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Triplet analysis that identifies unpaired regions of functional RNAs2011

    • Author(s)
      J.Kawakami, Y.Yamaguchi, N.Sugimoto
    • Journal Title

      J.Nucleic Acids

      Volume: 2011 Pages: 1-6

    • DOI

      10.4061/2011/471843

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effects of molecular crowding on the structure and stability of G-quadruplexes with an abasic site2011

    • Author(s)
      T.Fujimoto, S.Nakano, D.Miyoshi, N.Sugimoto
    • Journal Title

      J.Nucleic Acids

      Volume: 2011 Pages: 1-9

    • DOI

      10.4061/2011/857149

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of nucleic acid analogs for the study of nucleic acid interactions2011

    • Author(s)
      S.Nakano, M.Fujii, N.Sugimoto
    • Journal Title

      J.Nucleic Acids

      Volume: 2011 Pages: 1-11

    • DOI

      10.4061/2011/967098

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurements of the binding of a large protein using a substrate density-controlled DNA chip2011

    • Author(s)
      S.Nakano, T.Kanzaki, M.Nakano, D.Miyoshi, N.Sugimoto
    • Journal Title

      Anal.Chem.

      Volume: 83 Issue: 16 Pages: 6368-6372

    • DOI

      10.1021/ac201312d

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide2011

    • Author(s)
      S.Pramanik, K.Nakamura, K.Usui, S.Nakano, S.Saxena, J.Matsui, D.Miyoshi, N.Sugimoto
    • Journal Title

      Chem.Commun.

      Volume: 47 Pages: 2790-2792

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes.2010

    • Author(s)
      S. Saxena, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Biochemistry

      Volume: 49 Issue: 33 Pages: 7190-7201

    • DOI

      10.1021/bi101093a

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monomorphic RNA G-quadruplex and polymorphic DNA G-quadruplex structures responding to cellular environmental factors2010

    • Author(s)
      D. Zhang, T. Fujimoto, S. Saxena, H. Yu, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Biochemistry

      Volume: 49 Issue: 21 Pages: 4554-4563

    • DOI

      10.1021/bi1002822

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of new functional nanostructures consisting of both DNA duplex and quadruplex.2010

    • Author(s)
      K. Dutta, T. Fujimoto, M. Inoue, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Chem. Commun.

      Volume: 46 Issue: 41 Pages: 7772-7774

    • DOI

      10.1039/c0cc00710b

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anionic phthalocyanines targeting G-quadruplexes and inhibiting telomerase activity in the presence of excessive DNA duplexes2010

    • Author(s)
      H. Yaku, T. Murashima, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Chem. Commun.

      Volume: 46 Issue: 31 Pages: 5740-5742

    • DOI

      10.1039/c0cc00956c

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one goldnanoparticle-peptide-fluorophor e conjugate.2010

    • Author(s)
      X. Wang, J. Geng, D. Miyoshi, J. Ren, N. Sugimoto, and X. Qu
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 49 Issue: 2 Pages: 743-747

    • DOI

      10.1016/j.bios.2010.06.046

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ultrasensitive and selective detection of a prognostic indicator in early-stage cancer using graphene oxide and carbon nanotubes2010

    • Author(s)
      X. Wang, C. Wang, K. Qu, Y. Song, J. Ren, D. Miyoshi, N. Sugimoto, and X. Qu
    • Journal Title

      Adv. Funct. Mat.

      Volume: 20 Issue: 22 Pages: 3967-3971

    • DOI

      10.1002/adfm.201001118

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of salmon milt DNA against UV damage2010

    • Author(s)
      Y. Sasaki, D. Miyoshi, and N. Sugimoto
    • Journal Title

      Applied Biochem. Biotech.

      Volume: 160 Issue: 8 Pages: 4554-4563

    • DOI

      10.1007/s12010-009-8697-6

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides2010

    • Author(s)
      S. Nakano, H. Oka, Y. Uotani, K. Uenishi, M. Fujii, and N. Sugimoto
    • Journal Title

      Mol. BioSyst.

      Volume: 6 Issue: 10 Pages: 2023-2029

    • DOI

      10.1039/c0mb00002g

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH2010

    • Author(s)
      A. Rajendran, S. Nakano, and N. Sugimoto
    • Journal Title

      Chem. Commun.

      Volume: 46 Issue: 8 Pages: 1299-1301

    • DOI

      10.1039/b922050j

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of salmon milt DNA against UV damage2010

    • Author(s)
      Y.Sasaki, D.Miyoshi, N.Sugimoto
    • Journal Title

      Applied Biochem.Biotech.

      Volume: 160 Pages: 4554-4563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anionic phthalocyanines targeting G-quadruplexes and inhibiting telomerase activity in the presence of excessive DNA duplexes2010

    • Author(s)
      H.Yaku, T.Murashima, D.Miyoshi, N.Sugimoto
    • Journal Title

      Chem.Commun.

      Volume: 46 Pages: 5740-5742

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes2010

    • Author(s)
      S.Saxena, D.Miyoshi, N.Sugimoto
    • Journal Title

      Biochemistry

      Volume: 49 Pages: 4554-4563

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of new functional nanostructures consisting of both DNA duplex and quadruplex2010

    • Author(s)
      K.Dutta, T.Fujimoto, M.Inoue, D.Miyoshi, N.Sugimoto
    • Journal Title

      Chem.Commun.

      Volume: 46 Pages: 7772-7774

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultrasensitive and selective detection of a prognostic indicator in early-stage cancer using graphene oxide and carbon nanotubes2010

    • Author(s)
      X.Wang, C.Wang, K.Qu, Y.Song, J.Ren, D.Miyoshi, N.Sugimoto, X.Qu
    • Journal Title

      Adv.Funct.Mat.

      Volume: 20 Pages: 3967-3971

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rapid and sensitive "add-mix-measure" assay for multiple proteinases based on one goldnanoparticle-peptide-fluorophore conjugate2010

    • Author(s)
      X.Wang, J.Geng, D.Miyoshi, J.Ren, N.Sugimoto, X.Qu
    • Journal Title

      Biosens.Bioelectron.

      Volume: 49 Pages: 743-747

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides2010

    • Author(s)
      S.Nakano, H.Oka, Y.Uotani, K.Uenishi, M.Fujii, N.Sugimoto
    • Journal Title

      Mol.BioSyst.

      Volume: 6 Pages: 2023-2029

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular crowding of the cosolutes induces an intramolecular i-motif structure of triplet repeat DNA oligomers at neutral pH2010

    • Author(s)
      A.Rajendran, S.Nakano, N.Sugimoto
    • Journal Title

      Chem.Commun. 46

      Pages: 1299-1301

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes2009

    • Author(s)
      S. Nakano, H. T. Karimata, Y. Kitagawa, and N. Sugimoto
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 131 Issue: 46 Pages: 16881-16888

    • DOI

      10.1021/ja9066628

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An approach to peptide-based ATP receptors by a combination of random selection, rational design, and molecular imprinting2009

    • Author(s)
      J. Matsui, J. Nagano, D. Miyoshi, K. Tamaki, and N. Sugimoto
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 25 Issue: 3 Pages: 563-567

    • DOI

      10.1016/j.bios.2009.01.031

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stabilization of three-way junctions of DNA under molecular crowding conditions2009

    • Author(s)
      S. Muhuri, K. Mimura, D. Miyoshi, and N. Sugimoto
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 131 Issue: 26 Pages: 9268-9280

    • DOI

      10.1021/ja900744e

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions2009

    • Author(s)
      D. Miyoshi, K. Nakamura, H. T. Karimata, T. Ohmichi, and N. Sugimoto
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 131 Issue: 10 Pages: 3522-3531

    • DOI

      10.1021/ja805972a

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydration of Watson-Crick base pairs and dehydration of Hoogsteen base pairs inducing structural polymorphism under molecular crowding conditions2009

    • Author(s)
      D.Miyoshi, K.Nakamura, H.T.Karimata, T.Ohmichi, N.Sugimoto
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 3522-3531

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stabilization of three-way junctions of DNA under molecular crowding conditions2009

    • Author(s)
      S.Muhuri, K.Mimura, D.Miyoshi, N.Sugimoto
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 9268-9280

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes2009

    • Author(s)
      S.Nakano, H.T.Karimata, Y.Kitagawa, N.Sugimoto
    • Journal Title

      J.Am.Chem.Soc. 131

      Pages: 16881-16888

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An approach to peptide-based ATP receptors by a combination of random selection, rational design, and molecular imprinting2009

    • Author(s)
      J.Matsui, J.Nagano, D.Miyoshi, K.Tamaki, N.Sugimoto
    • Journal Title

      Biosens. Bioelectron. 25

      Pages: 563-567

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA junction structure stabilized by molecular crowding conditions2009

    • Author(s)
      D.Miyoshi, S.Muhuri, K.Mimura, N.Sugimoto
    • Journal Title

      Nucleic Acids Symp.Ser. 53

      Pages: 59-60

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Thermal stability and hydration state of DNA G-quadruplex regulated by loop regions2009

    • Author(s)
      T.Fujimoto, D.Miyoshi, H.T.Karimata, Naoki Sugimoto
    • Journal Title

      Nucleic Acids Symp.Ser. 53

      Pages: 237-238

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Investigations of the cation binding to nucleotides by monitoring the hairpin-duplex equilibrium of a self-complementary sequence2009

    • Author(s)
      S.Nakano, H.Hirayama, N.Sugimoto
    • Journal Title

      Nucleic Acids Symp.Ser. 53

      Pages: 519-520

    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(36)グラフェン酸化物ナノ表面におけるDNAの吸着・脱着機構の解明2012

    • Author(s)
      藤本健史、中野修一、杉本直己、三好大輔
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應大学(神奈川県)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(37)RNAヘアピン構造の二量化反応の効率を決める要因の解明2012

    • Author(s)
      中野修一、平山英伸、山口大輔、三好大輔、杉本直己
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應大学(神奈川県)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(31)RNase HとプローブRNAを用いたサイクリングプローブ技術によるPCRフリーのテロメラーゼ活性検出技術の開発2012

    • Author(s)
      夜久英信、村嶋貴之、三好大輔、杉本直己
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應大学(神奈川県)
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Contributions of water molecules to the reactions of cytoplasmic fatty acid binding proteins2011

    • Author(s)
      S.Nakano, S.Nagatoishi, N.Sugimoto, K.Mizutani
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Quantitative analysis of adsorption and desorption of DNA secondary structures on graphene oxide2011

    • Author(s)
      T. Fujimoto, S. Nakano, N. Sugimoto, and D. Miyoshi
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] PCR-free detection of telomerase activity in HeLa cells based on catalytic signal amplification with RNaseH2011

    • Author(s)
      H.Yaku, T.Murashima, D.Miyoshi, N.Sugimoto
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] A new fluorescent light-up probe for DNA2011

    • Author(s)
      D.Miyoshi, H.Yaku, T.Fujimoto, T.Murashima, N.Sugimoto
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Activity of the hammerhead ribozyme without divalent metal ions but in the presence of neutral additives2011

    • Author(s)
      S.Nakano, Y.Kitagawa, D.Miyoshi, Naoki Sugimoto
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] TPPリボスイッチを用いた新規アプタマーの合理的設計2011

    • Author(s)
      徳井美里、山内隆寛、三好大輔、西村顕、杉本直己
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 核酸のヘアピンループ構造形成における熱力学的安定性と水和の相関2011

    • Author(s)
      藤本健史、三好大輔、中野修一、杉本直己
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(筑波)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 擬似細胞核内環境下におけるアニオン性G-quadruplexリガンドのテロメラーゼ阻害効果2011

    • Author(s)
      夜久英信、村嶋之、三好大輔、杉本直巳
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(筑波)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脂肪酸結合タンパク質に結合する水分子の役割2011

    • Author(s)
      中野修一、長門石曉、杉本直己、水谷健一
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(筑波)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] G-quadruplex-ligands with negative charges under cell-mimicking conditions2011

    • Author(s)
      D.Miyoshi, H.Yaku, T.Murashima, N.Sugimoto
    • Organizer
      SECOND TRAINING SCHOOL on G-QUADRUPLEXES "Self-assembled guanosine structures for molecular electronic devices"
    • Place of Presentation
      Spa(ベルギー)(招待講演)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular crowding条件下における分子間相互作用の定量化2011

    • Author(s)
      川上純司、橋本阿弥、塚本隆広、岸本加恵、杉本直己
    • Organizer
      日本薬学会 第131年会
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 溶媒条件の変化がRNA-タンパク質相互作用へ与える影響2011

    • Author(s)
      田中裕子、石立和也、杉本直己、川上純司
    • Organizer
      日本薬学会 第131年会
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(21)人工核酸を使ったDNA構造の水和研究2011

    • Author(s)
      中野修一、山口大輔、三好大輔、松井淳、杉本直己
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(17)RNAとDNAのハイブリッド形成の熱力学に及ぼす共存分子の分子クラウディングの効果2011

    • Author(s)
      プラマニック スイッティモイ・長門石曉・杉本直己
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(19)RNAスリーウェイジャンクションの構造と熱力学的安定性に及ぼすブランチポイント近傍の塩基対の影響2011

    • Author(s)
      三村健太、三好大輔、中野修一、杉本直己
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(20)RNA二重らせん構造の熱力学的安定性及び水和における非塩基対領域の重要性2011

    • Author(s)
      藤本健史、三好大輔、中野修一、杉本直己
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌内でのRNA-タンパク質相互作用の定量化2010

    • Author(s)
      塚本隆広、橋本阿弥、岸本加恵、杉本直己、川上純司
    • Organizer
      第33回日本分子生物学会、第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] A New Functional Nanostructure Containing Both DNA Duplex and G-Quadruplex2010

    • Author(s)
      K.Dutta, T.Fujimoto, M.Inoue, D.Miyoshi, N.Sugimoto
    • Organizer
      Internationai Symposium on Advances in Nanomaterials (ANM 2010)
    • Place of Presentation
      India
    • Year and Date
      2010-12-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] ジャンクションポイント近傍の塩基対が制御するRNAスリーウェイジャンクションの形成2010

    • Author(s)
      三村健太、三好大輔、中野修一、杉本直己
    • Organizer
      第20回アンチセンスシンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Control of gene expression through an allosteric interaction of transactivator protein and its target RNA2010

    • Author(s)
      T. Endoh, and N. Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      はまぎんホール・ヴィアマーレ(横浜)
    • Year and Date
      2010-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Evaluation of the cation binding for the RNA dimerization under molecular crowding condition2010

    • Author(s)
      H. Hirayama, S. Nakano, D. Miyoshi, and N. Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      はまぎんホール・ヴィアマーレ(横浜)
    • Year and Date
      2010-11-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Roles of Anionic Functional Groups in G-quadruplex-ligands for Efficient Telomerase Inhibition2010

    • Author(s)
      H.Yaku, T.Murashima, D.Miyoshi, N.Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of LNA modification on the thermal stability of non-canonical DNA structures2010

    • Author(s)
      J.Bhattacharyya, S.Maiti, S.Muhuri, D.Miyoshi, N.Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Unpaired region affecting hydration state of duplexs2010

    • Author(s)
      T.Fujimoto, D.Miyoshi, S.Nakano, N.Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemishy 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evaluation of the cation binding for the RNA dimerization under molecular crowding condition2010

    • Author(s)
      H.Hirayama, S.Nakano, D.Miyoshi, N.Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemisky 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Magnesium ion binding to nucleotides : study with the hammerhead ribozyme2010

    • Author(s)
      S.Nakano, Y.Kitagawa, D.Miyoshi, N.Sugimoto
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative thermodynamic analysis of RNA-protein interaction on surface and in solution.2010

    • Author(s)
      Y.Tanaka, K.Ishidate, K.Kishimoto, N.Sugimoto, J.Kawakami
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] LNA/methyl phosphonate-DNAキメラオリゴヌクレオチドのmicroRNA阻害剤としての特性評価2010

    • Author(s)
      長濱宏治、伊勢田健太、Wengel Jesper、杉本直己、西方敬人、川上純司
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] RNAスリーウェイジャンクション構造における配列の重要性2010

    • Author(s)
      三村健太、三好大輔、中野修一、杉本直己
    • Organizer
      第4回バイオ関運化学シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA四重らせん構造とその熱力学的安定性に及ぼす塩基欠損部位の影響2010

    • Author(s)
      藤本健史、三好大輔、中野修一、杉本直己
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] RNA二量化反応に対するPEGによる分子クラウディング効果2010

    • Author(s)
      平山英伸、中野修一、三好大輔、杉本直己
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] G-quadruplex stability in the presence of its binding protein under a molecular crowding condition2010

    • Author(s)
      N.Sugimoto
    • Organizer
      The 19th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids (IRT 2010)
    • Place of Presentation
      France
    • Year and Date
      2010-09-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular crowding effect on functional nucleic acids2010

    • Author(s)
      N. Sugimoto
    • Organizer
      The 1st Asian Chemical Biology Conference(ACBC2010)
    • Place of Presentation
      ソウル大学(韓国)
    • Year and Date
      2010-06-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Molecular Crowding Effect on Functional Nucleic Acids2010

    • Author(s)
      N.Sugimoto
    • Organizer
      The 1st Asian Chemical Biology Conference (ACBC2010)
    • Place of Presentation
      Korea
    • Year and Date
      2010-06-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characters of Watson-Crick and Hoogsteen Base Pairs Inducing Structural Polymorphism under Molecular Crowding Conditions2010

    • Author(s)
      D.Miyoshi, K.Nakamura, H.Karimata, T.Ohmichi, N.Sugimoto
    • Organizer
      Gordon Research Conference (Biopolymers)
    • Place of Presentation
      USA
    • Year and Date
      2010-06-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stabilization and function of nucleic acids under molecular crowding conditions2010

    • Author(s)
      N. Sugimoto
    • Organizer
      Symposium on Nucleic Acid Chemistry(Structure and Interactions)
    • Place of Presentation
      Gorica(スロベニア)
    • Year and Date
      2010-05-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Stabilization and Function of Nucleic Acids under Molecular Crowding Conditions2010

    • Author(s)
      N.Sugimoto
    • Organizer
      Symposium on Nucleic Acid Chemistry (Structure and Interactions)
    • Place of Presentation
      Slovenia
    • Year and Date
      2010-05-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of molecular environments on the behaviors of biomolecules(9)Duplex formation of G-rich RNA sequences found in the 5'-untranslated region of protooncogenes2010

    • Author(s)
      サクセナサリカ, 三好大輔, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effect of molecular environments on the behaviors of biomolecules(12)Thermodynamic Stability of Hoogsteen and Watson-Crick Base Pairs in the Presence of Histone Mimicking Peptide2010

    • Author(s)
      プラマニックスイッティモイ, 中村かおり, 三好大輔, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(14)麹菌由来TPPリボスイッチを用いた新規アプタマーの開発2010

    • Author(s)
      徳井美里, 山内隆寛, 三好大輔, 神谷久弥, 松永將義, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(1)生体アニオン共存下におけるハンマーヘッドリボザイムの酵素活性2010

    • Author(s)
      北川雄一, 中野修一, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(2)ポリエチレングリコール存在下におけるRNAとカチオンの結合エネルギーの評価2010

    • Author(s)
      平山英伸, 中野修一, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(3)核酸に対する分子環境効果を調べるためのモデル研究2010

    • Author(s)
      中野修一, 平山英伸, 北川雄一, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(7)脱塩基を用いたDNA四重鎖のループ領域の定量的解析2010

    • Author(s)
      藤本健史, 三好大輔, 建石寿枝, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命分子の挙動に及ぼす分子環境の効果(8)RNAスリーウェイジャンクション構造に及ぼす分子クラウディングの影響2010

    • Author(s)
      三村健太, サンジュクタムフリ, 三好大輔, 杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] RNAの機能に及ぼす中性溶質の影響2009

    • Author(s)
      中野修一, 北川雄一, 平山英伸, 杉本直己
    • Organizer
      第32回溶液化学シンポジウム
    • Place of Presentation
      新潟
    • Year and Date
      2009-11-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Thermodynamic parameters for reliable prediction of RNA structures2009

    • Author(s)
      Y. Tanaka, K. Kishimoto, N. Sugimoto, and J. Kawakami
    • Organizer
      Joint Symposium of the 5th Annual Meeting of the Oligonucleotide Therapeutics Society and the 19th Antisense Symposium
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2009-11-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Thermodynamic parameters for reliable prediction of RNA structures2009

    • Author(s)
      Yuko Tanaka, Kae Kishimoto, Naoki Sugimoto, Junji Kawakami
    • Organizer
      Joint Symposium of the 5th Annual Meeting of the Oligonucleotide Therapeutics Society and the 19th Antisense Symposium
    • Place of Presentation
      福岡
    • Year and Date
      2009-11-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA junction structure stabilized by molecular crowding conditions2009

    • Author(s)
      D.Miyoshi, S.Muhuri, K.Mimura, N.Sugimoto
    • Organizer
      第6回国際核酸化学シンポジウム(第36回核酸化学シンポジウム)
    • Place of Presentation
      岐阜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Thermal stability and hydration state of DNA G-quadruplex regulated by loop regions2009

    • Author(s)
      T.Fujimoto, D.Miyoshi, H.Tateishi-Karimata, N.Sugimoto
    • Organizer
      第6回国際核酸化学シンポジウム(第36回核酸化学シンポジウム)
    • Place of Presentation
      岐阜
    • Year and Date
      2009-09-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Investigations of the cation binding to nucleotides by monitoring the hairpin-duplex equilibrium of a self-complementary sequence2009

    • Author(s)
      S.Nakano, H.Hirayama, N.Sugimoto
    • Organizer
      第6回国際核酸化学シンポジウム(第36回核酸化学シンポジウム)
    • Place of Presentation
      岐阜
    • Year and Date
      2009-09-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 分子クラウディング環境におけるDNAスリーウェイジャンクション構造の熱力学的安定性2009

    • Author(s)
      三村健太、サンジュクタムフリ、三好大輔、杉本直己
    • Organizer
      第24回生体機能関連化学シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNAの非標準型塩基対を利用した環境応答性ナノ構造体の構築2009

    • Author(s)
      藤本健史、カコリドッタ、井上真美子、三好大輔、杉本直己
    • Organizer
      第24回生体機能関連化学シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Stability of Watson-Crick and Hoogsteen base pairs of DNA under molecular crowding conditions2009

    • Author(s)
      D.Miyoshi, K.Nakamura, H.Tateishi-Karimata, T.Ohmichi, N.Sugimoto
    • Organizer
      42nd IUPAC Congress
    • Place of Presentation
      Glasgow, UK
    • Year and Date
      2009-08-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Formation of three-way junctions of DNA under molecular crowding conditions2009

    • Author(s)
      S.Muhuri, D.Miyoshi, N.Sugimoto
    • Organizer
      Gordon Research Conference(Nucleosides, Nucleotides & Oligonucleoties)
    • Place of Presentation
      Newport, RI
    • Year and Date
      2009-07-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Formation of three-way junctions of DNA under molecular crowding conditions2009

    • Author(s)
      S. Muhuri, D. Miyoshi, and N. Sugimoto
    • Organizer
      Gordon Research Conference(Nucleosides, Nucleotides & Oligonucleoties)
    • Place of Presentation
      Newport(アメリカ)
    • Year and Date
      2009-07-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 共存溶質によるテロメラーゼ活性の制御2009

    • Author(s)
      三好大輔, Hai-Quing Yu, 杉本直己
    • Organizer
      日本ケミカルバイオロジー学会第4回年会
    • Place of Presentation
      神戸市産業振興センター(神戸)
    • Year and Date
      2009-05-18
    • Related Report
      2011 Final Research Report
  • [Presentation] 共存溶質によるテロメラーゼ活性の制御2009

    • Author(s)
      三好大輔、Hai-QuingYu、杉本直己
    • Organizer
      日本ケミカルバイオロジー学会第4回年会
    • Place of Presentation
      神戸
    • Year and Date
      2009-05-18
    • Related Report
      2009 Annual Research Report
  • [Book] G-quartet, G-quadruplex, and G-wire regulated by chemical stimuli DNA2011

    • Author(s)
      D. Miyoshi and N. Sugimoto
    • Related Report
      2011 Final Research Report
  • [Book] 化学「グラフェンを用いたバイオセンサー」2011

    • Author(s)
      三好大輔、杉本直己
    • Total Pages
      6
    • Publisher
      化学同人
    • Related Report
      2011 Annual Research Report
  • [Book] Nanotechnology (Methods in Molecular Biology series) "G-quartet, G-quadruplex, and G-wire regulated by chemical stimuli DNA."2011

    • Author(s)
      D.Miyoshi, N.Sugimoto
    • Total Pages
      12
    • Publisher
      Springer
    • Related Report
      2011 Annual Research Report
  • [Book] バイオチップ実用化ハンドブック 第一節 DNAチップの基礎 1 ハイブリダイゼーション2010

    • Author(s)
      杉本直己・中野修一
    • Total Pages
      7
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Book] 現代化学 DNAの働きを制御する環境-分子クラウディングによる空間概念-2010

    • Author(s)
      小林克彰・杉本直己
    • Total Pages
      5
    • Publisher
      東京化学同人
    • Related Report
      2010 Annual Research Report
  • [Book] 化学フロンティア22 生命現象を理解する分子ツール最前線~イメージングから生体機能解析まで~18章 化学ツールとしての分子クラウディング2010

    • Author(s)
      中野修一・杉本直己
    • Total Pages
      9
    • Publisher
      化学同人
    • Related Report
      2010 Annual Research Report
  • [Book] シングルセル解析の最前線生体シグナル解析用の機能性核酸センサーの定量的デザイン2010

    • Author(s)
      中野修一・杉本直己
    • Total Pages
      8
    • Publisher
      シーエムシー
    • Related Report
      2009 Annual Research Report
  • [Book] 機能性核酸のテーラーメード設計超分子サイエンス & テクノロジー2009

    • Author(s)
      杉本直己, 三好大輔
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2011 Final Research Report
  • [Book] Chapter 8.Energy of Nucleic Acid Self-Assemblies : From Sequence to Function through Structure. Bottom-up Nanofabrication : Supramolecules, Self-Assemblies, and Organized Films2009

    • Author(s)
      S. Nakano and N. Sugimoto
    • Publisher
      American Scientific Publishers
    • Related Report
      2011 Final Research Report
  • [Book] Bottom-up Nanofabrication : Supramolecules, Self-Assemblies, and Organized Films Chapter 8. Energy of Nucleic Acid Self-Assemblies : From Sequence to Function through Structure2009

    • Author(s)
      S.Nakano, N.Sugimoto
    • Total Pages
      25
    • Publisher
      American Scientific Publishers
    • Related Report
      2009 Annual Research Report
  • [Book] 核酸医薬の最前線セントラルドグマに学ぶセントラルドグマの制御方法2009

    • Author(s)
      三好大輔、杉本直己
    • Total Pages
      13
    • Publisher
      シーエムシー
    • Related Report
      2009 Annual Research Report
  • [Book] 超分子サイエンス&テクノロジー機能性核酸のテーラーメード設計2009

    • Author(s)
      杉本直己、三好大輔
    • Total Pages
      9
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.konan-fiber.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.konan-fiber.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.konan-first.jp/database/search.php

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.konan-first.jp/database/search.php

    • Related Report
      2009 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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