Gene regulation via dynamic change of chromosomal conformation of remote enhancers
| Project/Area Number |
21247002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2011: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2010: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2009: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
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| Keywords | 遺伝学・ゲノム・進化・動物・発生 / 分化 / 遺伝 / ゲノム / 進化 / 動物 / 発生・分化 / 遺伝子発現制御 / 染色体ダイナミクス / 遠隔エンハンサー / 非翻訳保存配列 / 形態形成 / 進化発生学 |
|---|
| Research Abstract |
Sonic hedgehog(Shh) encodes a signaling protein that plays pivotal roles in vertebrate development. Comparison of genomic sequences across mammalian species and teleost fishes revealed four conserved non-coding sequences(CNCSs) that cluster in a region 600 to 900 kb upstream of the Shh promoter. These CNCSs drive regional transgenic lacZ reporter expression in the limb bud and the epithelial lining of the oral cavity, pharynx and larynx(lung/gut), recapitulating the endogenous Shh expression. Targeted elimination of each of the four CNCSs from the mouse genome results in loss of endogenous Shh expression in specific regions, corresponding to the expression pattern of the transgenic LacZ reporter, and leads to regional-specific defect. The results indicate that these CNCSs are tissue-specific long-range Shh enhancers. The 3D-FISH and 3C analyses showed that the Shh promoter-enhancer occurs in a tissue specific manner. Thus, chromosome dynamics is a common mechanism to regulate the tissue-specific Shh expression by the long-range enhancers.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development2009
Author(s)
Suzuki D, Yamada A, Amano T, Yasuhara R, Kimura A, Sakahara M, Tsumaki N, Takeda S, Tamura M, Nakamura M, Wada N, Nohno T, Shiroishi T, Aiba A, Kamijo R.
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Journal Title
Dev Biol
Volume: 335
Pages: 396-406
Related Report
Peer Reviewed
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[Journal Article] Identification and characterization of new long conserved noncoding sequences in vertebrates2009
Author(s)
Sakuraba Y, Kimura T, Masuya H, Noguchi H, Sezutsu H, Takahasi KR, Toyoda A, Fukumura R, Murata T, Sakaki Y, Yamamura M, Wakana S, Noda T, Shiroishi T, Gondo Y.
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Journal Title
Mammal Genome
Volume: 19
Pages: 703-712
Related Report
Peer Reviewed
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[Journal Article] Specific expression of Gsta4 in mouse cochlear melanocytes : a novel role for hearing and melanocyte differentiation2009
Author(s)
Uehara S, Izumi Y, Kubo Y, Wang CC, Mineta K, Ikeo K, Gojobori T, Tachibana M, Kikuchi T, Kobayashi T, Shibahara S, Taya C, Yonekawa H, Shiroishi T, Yamamoto H.
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Journal Title
Pigment Cell Melanoma Res
Volume: 22
Pages: 111-119
Related Report
Peer Reviewed
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