Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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Research Abstract |
DNA methylation and histone modifications are epigenetic marks that maintain an epigenetic state in each differentiated cell. We investigated structures and functions of two key proteins in maintenance and regulation of DNA methylation patterns ; a hemi-methylated DNA binding protein, UHRF1 and one of methyl CpG binding domain proteins, MBD4. Our structural and biochemical data have shed light on a combinatorial recognition of two histone modifications by UHRF1. Furthermore our research have gained structural basis of versatile base recognition of MBD4.
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