Actin filaments work as a negative tension sensor: elucidation of itsphysiochemical mechanism

• Project/Area Number: 21247021
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: SOKABE Masahiro 名古屋大学, 医学系研究科, 教授 (10093428)
• Co-Investigator(Kenkyū-buntansha): TATSUMI Hitoshi 名古屋大学, 医学系研究科, 准教授 (20171720)
• Co-Investigator(Renkei-kenkyūsha): HAYAKAWA Kimihide 名古屋大学, 工学研究科, 特任講師 (60467280)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000); Fiscal Year 2011: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2010: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2009: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
• Keywords: メカノセンサー / アクチン線維 / コフィリン / ゆらぎ / 1分子観察 / 分子動力学 / 伸展刺激 / 張力 / 回転ゆらぎ / 光ピンセット / 一分子観察 / 張力依存的結合 / 近接場光

Research Abstract

The actin depolymerizing factor (ADF) cofilin was found to bind selectively to relaxed actin filaments and severed them, indicating that actin filaments work as a negative tension sensor through a tension dependent regulation of cofilin activity. It is supposed that increased torsional fluctuations of relaxed actin filaments accelerate the cofilin binding to them.

Research Products

• [Journal Article] Imaging and characterization of stretch-induced ATP release from alveolar A549 cells. (2013)
  • Author(s): Grygorczyk R, Furuya K, Sokabe M
  • Journal Title: J Physiol Volume: 591 (5) Pages: 1195-1215
  • Peer Reviewed
• [Journal Article] Mechanosensing by actin filaments and focal proteins. (2012)
  • Author(s): Hayakawa K, Tatsumi H, Sokabe M
  • Journal Title: Commun Integr Biol Volume: 5(6) Pages: 572-577
  • Peer Reviewed
• [Journal Article] The gating mechanism of the bacterial mechanosensitive channel MscL revealed by molecular dynamics simulations:from tension sensing to channel opening. (2012)
  • Author(s): Sawada Y, Murase M, Sokabe M
  • Journal Title: From tension sensing to channel opening. Channels Volume: 6(4) Pages: 317-331
  • Peer Reviewed
• [Journal Article] STIM1 regulates platelet-derived growth factor-induced migration and Ca2+ influx in human airway smooth muscle cells. (2012)
  • Author(s): Suganuma N, Ito S, Aso H, Kondo M, Sato M, Sokabe M, Hasegawa Y.
  • Journal Title: PloS ONE Volume: 7(9)
  • Peer Reviewed
• [Journal Article] Differential effects of lipids and lyso-lipids on the mechanosensitivity of MscL and MscS (2012)
  • Author(s): Nomura T, Cranfield CG, Deplazes E, Owen DM, Macmillan A, Battle AR, Constantine M, Sokabe M, Martinac B
  • Journal Title: PNAS Volume: 109(22) Pages: 8770-8775
  • Peer Reviewed
• [Journal Article] Mechanosensing by actin filaments and focal proteins (2012)
  • Author(s): Hayakawa K, Tatsumi H, Sokabe M
  • Journal Title: Commun Integr Biol Volume: 5(6) Issue: 6 Pages: 572-576
  • DOI: 10.4161/cib.21891
  • Peer Reviewed
• [Journal Article] Actin filaments function as a tension sensor via tension-dependent binding of cofilin to the filament. (2011)
  • Author(s): Hayakawa K, Tatsumi H, Sokabe M.
  • Journal Title: J Cell Biol Volume: 195(5) Pages: 721-727
  • Peer Reviewed
• [Journal Article] Force- and Ca^-dependent internalization of integrin in cultured endothelial cells. (2011)
  • Author(s): Kiyoshima D, Kawakami K, Hayakawa K,Tatsumi H, Sokabe M.
  • Journal Title: J Cell Sci Volume: 124(Pt 22) Pages: 3859-70
  • Peer Reviewed
• [Journal Article] Visualization of flow-induced ATP release and triggering of Ca^ waves at caveolae in vascular endothelial cells. (2011)
  • Author(s): Yamamoto K, Furuya K, Nakamura M, Kobatake E, Sokabe M, Ando J.
  • Journal Title: J Cell Sci Volume: 124 Pages: 3477-83
  • Peer Reviewed
• [Journal Article] Effect of tensile force on the mechanical be behaviors of actin filaments. (2011)
  • Author(s): Matsushita S, Inoue Y, Hojo M, Sokabe M, Adachi T.
  • Journal Title: J,Biomech Volume: 4 4(9) Pages: 1776-81
  • Peer Reviewed
• [Journal Article] Mechanoreception by Motile Flagella revealed by Chlamydomonas (2011)
  • Author(s): Fujiu K, Nakayama Y, Iida H, Sokabe M, Yoshimura K.
  • Journal Title: Nature Cell Biol Volume: 13(5) Pages: 630-632
  • Peer Reviewed
• [Journal Article] Protective role of Gipie, a Girdinfamily protein, in endoplasmic reticulum stress responses in endothelial cells (2011)
  • Author(s): Matsushita E, Asai N, Enomoto A, Kawamoto Y, Kato T, Maeda K, Hattori S, Hagikura M, Takahashi K, Sokabe M, Murakumo Y, Murohara T, Takahashi M
  • Journal Title: Mol Biol Cell Volume: 22(6) Pages: 736-747
  • Peer Reviewed
• [Journal Article] Actin filaments function as a tension sensor via tension-dependent binding of cofilin to the filament (2011)
  • Author(s): Hayakawa K, Tatsumi H, Sokabe M
  • Journal Title: J Cell Biol Volume: 195(5) Issue: 5 Pages: 721-727
  • DOI: 10.1083/jcb.201102039
  • Peer Reviewed
• [Journal Article] Effect of tensile force on the mechanical behaviors of actin filaments (2011)
  • Author(s): Matsushita S, Inoue Y, Hojo M. Sokabe M, Adachi T
  • Journal Title: J Biomech Volume: 44(9) Pages: 1776-1781
  • NAID: 120003779176
  • Peer Reviewed
• [Journal Article] Evaluation of Extensional and Torsional Stiffness of Single Actin Filaments by Molecular Dynamics Analysis (2010)
  • Author(s): Matsushita S, Adachi T, Inoue Y, Hojo M. Sokabe M.
  • Journal Title: J Biomech Volume: 43(16) Pages: 3162-7
  • NAID: 120002906762
  • Peer Reviewed
• [Journal Article] Sensing substrate rigidity y mechanosensitive ion channels with stress fibers and focal adhesions. (2010)
  • Author(s): Kobayashi T, Sokabe M.
  • Journal Title: Curr Opin Cell Biol Volume: 22 Pages: 669-676
  • Peer Reviewed
• [Journal Article] Mechanosensitivity of ion channels based on protein-lipid interaction. (2010)
  • Author(s): Yoshimura K, Sokabe M
  • Journal Title: J Royal Soc Interface Volume: 7
  • Peer Reviewed
• [Journal Article] Evaluation of Extensional and Torsional Stiffness of Single Actin Filaments by Molecular Dynamics Analysis. (2010)
  • Author(s): Matsushita S, Adachi T, Inoue Y, Hojo M.Sokabe M
  • Journal Title: J Biomech Volume: 43(16) Pages: 3162-3167
  • NAID: 120002906762
  • Peer Reviewed
• [Journal Article] Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions. (2010)
  • Author(s): Kobayashi T, Sokabe M.
  • Journal Title: Curr Opin Cell Biol Volume: 22 Pages: 669-676
  • Peer Reviewed
• [Journal Article] Actin cytoskeleton regulates stretch-activated Ca^<2+> influx in human lung microvascular endothelial cells. (2010)
  • Author(s): Ito S, Kume H, Numaguchi Y, Ishii M, Iwaki M, Kondo M, Naruse K, Hasegawa Y, Sokabe M
  • Journal Title: Am J Respir Cell Mol Biol Volume: 43(1) Pages: 26-34
  • Peer Reviewed
• [Journal Article] Involvement of PI3K/Akt/TOR pathway in stretch-induced hypertrophy in primary cultured skeletal myotubes (2010)
  • Author(s): Sasai N, Agata N, Inoue-Miyazu M, Kawakami K, Kobayashi K, Sokabe M, Hayakawa K
  • Journal Title: Nerve Muscle Volume: 41(1) Pages: 100-106
  • Peer Reviewed
• [Journal Article] Structural changes in the cytoplasmic domain of the mechanosensitive channel MscS during opening (2009)
  • Author(s): Machiyama H, Tatsumi H, Sokabe M
  • Journal Title: Biophys J Volume: 97 Pages: 1048-1057
  • Peer Reviewed
• [Journal Article] Stress-Axis Regulated Exon (STREX) in the C-terminus of BKCa channels is responsible for the stretch sensitivity. (2009)
  • Author(s): Naruse K, Tang QY, Sokabe M
  • Journal Title: Biochem Biophys Res Com Volume: 385(4) Pages: 634-639
  • Peer Reviewed
• [Journal Article] Microtubule dydynamics regulate cyclic str etceh-induced cell reorientation in human airway smooth muscle cells.
  • Author(s): Morioka M, Parameswarane H, Naruse K, Kondo M, Sokabe M, Hasegawa Y, Suki B, Ito S.
  • Journal Title: PLoS One Volume: 6(10)
  • Peer Reviewed
• [Presentation] Mechanotransduction by actin filaments is realized by tension dependent cofilin severing of actin filaments (2013)
  • Author(s): SokabeM
  • Organizer: 14th International Membrane Research Forum: Meso-Scale Molecular Complexes and Domains and their Functions in the Membrane
  • Place of Presentation: iCeM, Kyoto, Japan
• [Presentation] 細胞力覚研究におけるイオンコンダクタンス顕微鏡(ICM)の有用性と発展性 (2012)
  • Author(s): 曽我部正博
  • Organizer: 第4回プローブ顕微鏡による表面分析研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-02-10
• [Presentation] Critical role of mechanosensitive ATP release from mammary alveoli in milk ejection. Symposium on "Emerging Roles of Purinergic Signaling in Mechanobiology" (2012)
  • Author(s): Sokabe M, Furuya K
  • Organizer: Purine 2012 in Fukuoka
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Roles of actin cytoskeleton in cellular mechanotransduction (2012)
  • Author(s): Sokabe M
  • Organizer: Symposium on "Force Transduction and Emerging Channels"
  • Place of Presentation: Berlin, Germany
• [Presentation] 細胞力覚の生物物理学と比較生理学、シンポジウム「メカノトランスダクションと生理機能」 (2012)
  • Author(s): 曽我部正博
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
• [Presentation] 開会挨拶:"生物に学ぶ柔軟なシステムの探索:ゆらぎと多様性をキーワードとして"の主旨 (2011)
  • Author(s): 曽我部正博
  • Organizer: 日本学術会議・学術フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-01
• [Presentation] 神経ステロイドの神経保護作用:アルツハイマー病と脳卒中の新規治療法の可能性 (2011)
  • Author(s): 曽我部正博
  • Organizer: Replacement Therapy 研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-08
• [Presentation] Comparative biophysics and physiology of cell mechanosensing: frompassive to actove touch (2011)
  • Author(s): Sokabe M
  • Organizer: 8th Internationa Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2011-06-01
• [Presentation] How do forces activate mechanosensitive channels in bacteria and eukaryotic cells? Internatl Symposium on Mechanobiology (2011)
  • Author(s): Sokabe M
  • Organizer: 5th Shanghai International Conference on Biophysics and Molecular biology.
  • Place of Presentation: Shanghai/Hangzhou, China.
• [Presentation] Application of optical recording technique with voltage sensitive dyes to the study of synaptic transmission and plasticity in the brain (2011)
  • Author(s): Sokabe M
  • Organizer: SERC School on "Biophysics in Medicine: Advanced Training in imaging of Experimental Models"
  • Place of Presentation: Delhi, India
• [Presentation] Biophysical and Evolutionary Aspects of Cell mechanosensing by Mechanosensitive Ion Channels (2011)
  • Author(s): Sokabe M
  • Organizer: 7th ABA (Asian Biophysics Association) Symposium.
  • Place of Presentation: Delhi, India
• [Presentation] 細胞の重力感知をめぐる諸問題と解決の方向性。シンポジウム"今、改めて考える宇宙生物科学" (2011)
  • Author(s): 曽我部正博
  • Organizer: 第2 5回宇宙生物科学会大会
  • Place of Presentation: 横浜
• [Presentation] 神経ステロイドによるアルツハイマー病の予防・治療の可能性 (2011)
  • Author(s): 曽我部正博
  • Organizer: 生理学研究所研究会"超階層シグナル伝達研究の新展開
  • Place of Presentation: 岡崎
• [Presentation] The Impact of Emerging Nano-Mechanobiology (2010)
  • Author(s): Sokabe M
  • Organizer: Nagoya University FIRST Research Center for Innovative Nanobiodevice International Symposium
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2010-11-15
• [Presentation] Multiple roles of actin cytoskeleton in cell mechanosensing. (2010)
  • Author(s): Sokabe M.Hayakawa K, Tatsumi H, Kobayashi T
  • Organizer: 6th World Congress on Biomechanics
  • Place of Presentation: Singapore
  • Year and Date: 2010-08-04
• [Presentation] Comparative Biophysics of Mechanotransduction: from bacteria to endothelial cells (2010)
  • Author(s): Sokabe M
  • Organizer: The 1st International Symposium on Biorheology
  • Place of Presentation: Wako, Japan
  • Year and Date: 2010-06-02
• [Presentation] Nano-Mechanobiology: Sensing Mechanical Stimuli and Substrate Rigidity (2010)
  • Author(s): Sokabe M
  • Organizer: NanoBioMedicine Symposium
  • Place of Presentation: Nagoya, Japan
• [Presentation] Mechanotransduction at cell membrane and cell-substrate adhesion (2010)
  • Author(s): Sokabe M
  • Organizer: The 6th GEM4 Summer School on "Cell-cell and cell-substrate adhesion.
  • Place of Presentation: Singapore
• [Presentation] Mehanosensing by ion channels: from bacteria to human. Symposium on " Emerging Mechanobiology" (2010)
  • Author(s): 曽我部正博
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
• [Presentation] Role of cytoskeleton in mechanotransduction. (2009)
  • Author(s): Sokabe M
  • Organizer: Gordon Research Conference : Osmoregulation and Mechanotransduction.
  • Place of Presentation: New England, USA
  • Year and Date: 2009-07-07
• [Presentation] Nano-Mechanobiology: Sensing Mechanical Stimuli and Substrate Rigidity
  • Author(s): Sokabe M
  • Organizer: Nano・Biomedicine Sympojium at 4th Ann Met Nanobiomedical Soc
  • Place of Presentation: Nagoya, Japan
• [Presentation] Mechanotransduction by actin filaments is realized by tension dependent cofilin severing of actin filaments.
  • Author(s): Sokabe M
  • Organizer: 14th Internatl Membrane Research Forum
  • Place of Presentation: Kyoto/Japan
  • Invited
• [Book] A Note on Emerging Mechanobiology. In "Recent Advances in Mechanobiology" (Eds. Yongde, Sokabe, et al) (2012)
  • Author(s): Sokabe M.
  • Publisher: Shanghai Sci Tech Liter Pub House
• [Book] How do forces activate mechanosensitive ion channels in bacterial and eukaryotic cells? (2012)
  • Author(s): Sokabe M, Sawada Y, Nomura K, Yoshimura K, Kobayashi T
  • Publisher: Shanghai Sci Tech Liter Pub House
• [Book] The role of actin filaments as a force-transmitter and a force-sensor. (2012)
  • Author(s): Tatsumi H, Hayakawa K, Hirata H, Sokabe M.
  • Publisher: Shanghai Sci Tech Liter Pub House
• [Book] Recent Advances in Mechanobiology (2012)
  • Author(s): Shi Y, Sokabe M, et al. (eds.)
  • Total Pages: 201
  • Publisher: Shanghai Sci Tech Liter Pub House
• [Book] Time-window of progesterone neuroprotection after stroke. In "Cerebral Ischemia/Book 1", (Ed. Balestrino M) (2012)
  • Author(s): Cai W, Sokabe M, Chen L
  • Publisher: InTech
• [Book] Methods for processing and analyzing single channel data. In "Modern Patch Clamp Techniques" (Ed. Okada Y) (2012)
  • Author(s): Sokabe M
  • Publisher: Springer Verlag
• [Book] Modern Patch Clamp Techniques （Ed. Okada Y） (2012)
  • Author(s): Sokabe M
  • Total Pages: 452
  • Publisher: Springer verlag pp85-104, Methods for processing and analyzing single channel data.
• [Book] 細胞はどのように力を感じるのか:細胞力覚研究の最前線、In「次世代バイオミメティクス研究の最前線」(下村編) (2011)
  • Author(s): 曽我部正博
  • Publisher: シーエムシー出版
• [Book] Nanotechnology in mechanobiology: mechanical manipulation of cells and organelle while monitoring intracellular signaling. In "Mechanosensing Biology" (ed. Noda M) (2010)
  • Author(s): Tatsumi H, Hayakawa K, Sokabe M.
  • Publisher: Springer Verlag
• [Book] 第1 部 生物学序論:細胞と生理学概論、In「ガイトン生理学(11 版)」(原題:Introduction to Physiology:The Cell and General Physiology, In "Textbook of Medical Physiology, Guyton & Hall") (2010)
  • Author(s): 曽我部正博(鑑訳)
  • Publisher: エルセビアJP
• [Book] Mechanosensing Biology(Ed.Noda M) (2010)
  • Author(s): Tatsumi H, Hayakawa K, Sokabe M
  • Total Pages: 219
  • Publisher: Springer verlag pp3-45, Nanotechnology in mechanobiology : mechanical manipulation of cells and organelle while monitoring intracellular signaling
• [Book] 動物は何を考えているのか?:学習と記憶の比較生物学 (2009)
  • Author(s): 曽我部正博(編著)
  • Total Pages: 260
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/physiol2/
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/physiol2/