Novel functions of nuclear transport factors : stress-response mechanism of cell nucleus

• Project/Area Number: 21247032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: YONEDA Yoshihiro 大阪大学, 生命機能研究科, 教授 (80191667)
• Co-Investigator(Renkei-kenkyūsha): OKA Masahiro 大阪大学, 生命機能研究科, 助教 (40432504)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000); Fiscal Year 2011: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2010: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2009: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
• Keywords: 核輸送 / ストレス応答 / importinα / 細胞核 / 遺伝子発現 / 細胞死 / importin α / マイクロアレイ

Research Abstract

Various cellular stresses induce the nuclear accumulation of importinα. However, it is unknown whether accumulated importinαperforms roles in the nucleus. In this study, we found that nuclear-retained importinα2binds with DNase I-sensitive nuclear component(s) and exhibits selective up-regulation of mRNA encoding Serine/ threonine kinase35(STK35). Chromatin immunoprecipitation and promoter analysis demonstrated that importinα2binds to the promoter region of STK35and accelerates its transcription. Furthermore, constitutive overexpression of STK35proteins enhances caspase-independent cell death under oxidative stress conditions. These results reveal that nuclear-localized importinα2influences gene expression and contributes directly to cell fate outcomes including non-apoptotic cell death. This is the first evidence showing that importinαhas novel functions other than nuclear protein import in the nucleus.

Research Products

• [Journal Article] The interaction between importin-alpha and Nup153promotes importin-alpha/ beta-mediated nuclear import (2012)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y
  • Journal Title: Traffic Volume: (in press)
  • Peer Reviewed
• [Journal Article] Intrinsic and extrinsic negative regulators of nuclear protein transport processes (2012)
  • Author(s): Sekimoto, T. and Yoneda, Y
  • Journal Title: Genes Cells Volume: (in press)
  • Peer Reviewed
• [Journal Article] The small GTPase Ran and Ran-binding proteins (2012)
  • Author(s): Nagai, M. and Yoneda, Y
  • Journal Title: BioMol. Concepts Volume: (in press)
  • Peer Reviewed
• [Journal Article] Nuclear importinαand its physiological importance (2012)
  • Author(s): Miyamoto, Y., Loveland, K. L. and Yoneda, Y
  • Journal Title: Commun. Integ. Biol. Volume: 5 Pages: 220-222
  • URL: http://dx.doi.org/10.1038/emboj.2011.360
  • Peer Reviewed
• [Journal Article] The role of Rae1 in Nup98-fusion mediated leukemogenesis (2011)
  • Author(s): Oka, M. and Yoneda, Y
  • Journal Title: Cell Cycle Volume: 10 Pages: 12-12
  • URL: http://dx.doi.org/10.4161/cc.10.12.15759
  • Peer Reviewed
• [Journal Article] Nucleo-cytoplasmic transport of microRNAs and related small RNAs (2011)
  • Author(s): Katahira, J. and Yoneda, Y
  • Journal Title: Traffic Volume: 12 Issue: 11 Pages: 1468-1474
  • DOI: 10.1111/j.1600-0854.2011.01211.x
  • Peer Reviewed
• [Journal Article] Nuclear retention of importinαcoordinates cell fate through changes in gene expression (2011)
  • Author(s): Yasuda, Y., Miyamoto, Y., Yamashiro, T., Asally, M., Masui, A., Loveland, K. L. and Yoneda, Y
  • Journal Title: EMBO J. Volume: 31 Issue: 1 Pages: 83-94
  • DOI: 10.1038/emboj.2011.360
  • Peer Reviewed
• [Journal Article] Cell type-dependent gene regulation by Staufen2in conjunction with Upf1 (2011)
  • Author(s): Miki, T., Kurono, S., Kaneko, Y., Katahira, J. and Yoneda, Y
  • Journal Title: BMC Mol. Biol. Volume: 12 Issue: 1 Pages: 48-48
  • DOI: 10.1186/1471-2199-12-48
  • Peer Reviewed
• [Journal Article] Stimulation of CK2-dependent Grp94phosphorylation by the nuclear localization signal peptide (2011)
  • Author(s): Miyata, Y., Yoneda, Y. and Yahara, I
  • Journal Title: Mol. Cell. Biochem. Volume: 356 Issue: 1-2 Pages: 191-200
  • DOI: 10.1007/s11010-011-0944-9
  • Peer Reviewed
• [Journal Article] Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47in conjunction with its partnertranscription factors (2011)
  • Author(s): Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachinbana, T and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3715-3724
  • Peer Reviewed
• [Journal Article] Cell type-specific transcriptional regulation of the gene encoding importin-α (2011)
  • Author(s): Kamikawa, Y., Yasuhara, N. and Yoneda, Y
  • Journal Title: Exp. Cell Res. Volume: 317 Pages: 1970-1978
  • URL: http://dx.doi.org/10.1016/j.yexcr.2011.05.024
  • Peer Reviewed
• [Journal Article] Targeted disruption of one of the importinαfamily members leads to female functional incompetence in delivery (2011)
  • Author(s): Moriyama, T., Nagai, M., Oka, M., Ikawa, M., Okabe, M. and Yoneda, Y
  • Journal Title: FEBS J. Volume: 278 Pages: 1561-1572
  • Peer Reviewed
• [Journal Article] Generation and characterization of a monoclonal antibody against importinα7/ NPI-2 (2011)
  • Author(s): Mizuguchi, C., Moriyama, T. and Yoneda, Y
  • Journal Title: Hybridoma Volume: 30 Issue: 3 Pages: 307-309
  • DOI: 10.1089/hyb.2011.0006
  • Peer Reviewed
• [Journal Article] Generation of rat monoclonal antibody specific for mouse importinα8 (2011)
  • Author(s): Moriyama, T., Mizuguchi, C. and Yoneda, Y
  • Journal Title: Hybridoma Volume: 30 Issue: 3 Pages: 303-306
  • DOI: 10.1089/hyb.2011.0004
  • Peer Reviewed
• [Journal Article] Camoretti-Mercado, B. and Solway, J. Nuclear import of serum response factor in airway smooth mascle (2011)
  • Author(s): McConville, J. F., Fernandes, D. J., Churchill, J., Dewundara, S., Kogut, P., Shah, S., Fuchs, G., Kedainis, D., Bellam, S. K., Patel, N. M., McCauley, J., Dulin, N. O., Gupta, M. P., Adam, S., Yoneda, Y
  • Journal Title: Am J. Respir. Cell Mol. Biol. Volume: 45 Issue: 3 Pages: 453-458
  • DOI: 10.1165/rcmb.2008-0393oc
  • Peer Reviewed
• [Journal Article] Importinαprotein acts as a negative regulator for Snail protein nuclear import (2011)
  • Author(s): Sekimoto, T., Miyamoto, Y., Arai, S. and Yoneda, Y
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 17 Pages: 15126-15131
  • DOI: 10.1074/jbc.m110.213579
  • Peer Reviewed
• [Journal Article] Proteomic Analysis of Importin .ALPHA.-interacting Proteins in Adult Mouse Brain (2011)
  • Author(s): Fukumoto, M., Sekimoto, T. and Yoneda, Y
  • Journal Title: Cell Structure and Function Volume: 36 Issue: 1 Pages: 57-67
  • DOI: 10.1247/csf.10026
  • NAID: 130004137564
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Mice lacking Ran Binding Protein 1 are viable and show male infertility (2011)
  • Author(s): Nagai, M., Moriyama, T., Mehmood, R., Tokuhiro, K., Ikawa, M., Okabe, M., Tanaka, H. and Yoneda, Y
  • Journal Title: FEBS Lett. Volume: 585 Pages: 791-796
  • URL: http://dx.doi.org/10.1016/j.febslet.2011.02.002
  • Peer Reviewed
• [Journal Article] Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importinβ (2011)
  • Author(s): Ohara, R., Hata, K., Yasuhara, N., Mehmood, R., Yoneda, Y., Nakagawa, M. and Yamashita, T
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 405 Issue: 4 Pages: 697-702
  • DOI: 10.1016/j.bbrc.2011.01.108
  • Peer Reviewed
• [Journal Article] NUP358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure during skeletal myogensis (2011)
  • Author(s): Asally, M., Yasuda, Y., Oka, M., Otsuka, S., Yoshimura, S. H., Takeyasu, K. and Yoneda, Y
  • Journal Title: FEBS J. Volume: 278 Issue: 4 Pages: 610-621
  • DOI: 10.1111/j.1742-4658.2010.07982.x
  • Peer Reviewed
• [Journal Article] Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery (2011)
  • Author(s): Moriyama, T., et al
  • Journal Title: FEBS J. Volume: 278 Issue: 9 Pages: 1561-1572
  • DOI: 10.1111/j.1742-4658.2011.08079.x
  • Peer Reviewed
• [Journal Article] Cell type-specific transcriptional regulation of the gene encoding importin-α1 (2011)
  • Author(s): Kamikawa, Y., Yasuhara, N., Yoneda, Y.
  • Journal Title: Exp.Cell Res. Volume: 317 Issue: 14 Pages: 1970-1978
  • DOI: 10.1016/j.yexcr.2011.05.024
  • Peer Reviewed
• [Journal Article] Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors (2011)
  • Author(s): Mehmood, R., et al
  • Journal Title: Mol.Biol.Cell Volume: 22 Issue: 19 Pages: 3715-3724
  • DOI: 10.1091/mbc.e10-10-0809
  • Peer Reviewed
• [Journal Article] The role of Rael in Nup98-fusion mediated leukemogenesis (2011)
  • Author(s): Oka, M., Yoneda, Y.
  • Journal Title: Cell Cycle. Volume: 10 Issue: 12 Pages: 12-12
  • DOI: 10.4161/cc.10.12.15759
  • Peer Reviewed
• [Journal Article] The interaction between importin-alpha and Nup153 promotes importin-alpha/beta-mediated nuclear import (2011)
  • Author(s): Ogawa, Y., et al
  • Journal Title: Traffic Volume: (In press)
  • Peer Reviewed
• [Journal Article] Nuclear importin α and its physiological importance (2011)
  • Author(s): Miyamoto, Y., et al
  • Journal Title: Commun.Integ.Biol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Intrinsic and extrinsic negative regulators of nuclear protein transport processes (2011)
  • Author(s): Sekimoto, T., Yoneda Y.
  • Journal Title: Genes Cells Volume: (In press)
  • Peer Reviewed
• [Journal Article] The small GTPase Ran and Ran-binding proteins (2011)
  • Author(s): Nagai, M., Yoneda, Y.
  • Journal Title: BioMol.Concepts Volume: (In press)
  • Peer Reviewed
• [Journal Article] Efficient generation of stable induced pluripotent stem cells. (2011)
  • Author(s): Okada, M., Yoneda, Y.
  • Journal Title: Biochim.Biophys.Acta-General Subjects Volume: 1810 Pages: 226-235
  • Peer Reviewed
• [Journal Article] NUP358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure during skeletal myogensis. (2011)
  • Author(s): Asally, M., et al.
  • Journal Title: FEBS J Volume: 278 Pages: 610-621
  • Peer Reviewed
• [Journal Article] Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin β. (2011)
  • Author(s): Ohara, R., et al.
  • Journal Title: Biochem.Biophys.Res.Commun Volume: 405 Pages: 697-702
  • Peer Reviewed
• [Journal Article] Nuclear import of serum response factor in airway smooth mauscle. (2011)
  • Author(s): McConville, J.F., et al.
  • Journal Title: Am J.Respir.Cell Mol.Biol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Proteomic analysis of importin α-interacting proteins in adult mouse brain. (2011)
  • Author(s): Fukumoto, M., et al.
  • Journal Title: Cell Struct.Funct Volume: (In press)
  • Peer Reviewed
• [Journal Article] Mice lacking Ran Binding Protein 1 are viable and show female infertility. (2011)
  • Author(s): Nagai, M., et al.
  • Journal Title: FEBS Lett Volume: (In press)
  • Peer Reviewed
• [Journal Article] Generation and characterization of a monoclonal antibody against importin α7/NPI-2. (2011)
  • Author(s): Mizuguchi, C., et al.
  • Journal Title: Hybridoma Volume: (In press)
  • Peer Reviewed
• [Journal Article] Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery. (2011)
  • Author(s): Moriyama, T., et al.
  • Journal Title: FEBS J Volume: (In press)
  • Peer Reviewed
• [Journal Article] Generation of rat monoclonal antibody specific for mouse importin α8 (2011)
  • Author(s): Moriyama, T., et al.
  • Journal Title: Hybridoma Volume: (In press)
  • Peer Reviewed
• [Journal Article] Importin α acts as a negative regulator for Snail nuclear import. (2011)
  • Author(s): Sekimoto, T., et al.
  • Journal Title: J.Biol.Chem Volume: (In press)
  • Peer Reviewed
• [Journal Article] Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons (2010)
  • Author(s): Oe, S. and Yoneda, Y
  • Journal Title: FEBS Lett. Volume: 584 Pages: 3424-3430
  • URL: http://dx.doi.org/10.1016/j.febslet.2010.06.040
  • Peer Reviewed
• [Journal Article] Effective culture conditions for the induction of pluripotent stem cells (2010)
  • Author(s): Okada, M., Oka, M. and Yoneda, Y
  • Journal Title: Biochim. Biophys. Acta-General Subjects Volume: 1800 Pages: 956-963
  • URL: http://dx.doi.org/10.1016/j.bbagen.2010.04.004
  • Peer Reviewed
• [Journal Article] Identification of importinαl as a novel constituent of RNA stress granules (2010)
  • Author(s): Fujimura, K., Suzuki, T., Yasuda, Y., Murata, M., Katahira, J. and Yoneda, Y
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1803 Pages: 865-871
  • URL: http://dx.doi.org/10.1016/j.bbamcr.2010.03.020
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export (2010)
  • Author(s): Oka, M., Asally, M., Yasuda, Y., Tachibana, T. and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 21 Issue: 11 Pages: 1885-1896
  • DOI: 10.1091/mbc.e09-12-1041
  • Peer Reviewed
• [Journal Article] Two isoforms of Npap60(Nup50) differentially regulate nuclear protein import (2010)
  • Author(s): Ogawa, Y., Miyamoto, Y., Asally, M., Oka, M., Yasuda, Y. and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 21 Issue: 4 Pages: 630-638
  • DOI: 10.1091/mbc.e09-05-0374
  • Peer Reviewed
• [Journal Article] Two isoforms of Npap60 (Nup50) differentially regulate nuclear protein import. (2010)
  • Author(s): Ogawa, Y., et al.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 630-638
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. (2010)
  • Author(s): Oka, M., et al.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 1885-1896
  • Peer Reviewed
• [Journal Article] Identification of importin α1 as a novel constituent of RNA stress granules. (2010)
  • Author(s): Fujimura, K., et al.
  • Journal Title: Biochim.Biophys.Acta-Molecular Cell Research Volume: 1803 Pages: 865-871
  • Peer Reviewed
• [Journal Article] Effective culture conditions for the induction of pluripotent stem cells. (2010)
  • Author(s): Okada, M., et al.
  • Journal Title: Biochim.Biophys.Acta-General Subjects Volume: 1800 Pages: 956-963
  • Peer Reviewed
• [Journal Article] Cytoplasmic polyadenylation element-like sequences are involved in dendritic targeting of BDNF mRNA in hippocampal neurons. (2010)
  • Author(s): Oe, S., Yoneda, Y.
  • Journal Title: FEBS Lett Volume: 584 Pages: 3424-3430
  • Peer Reviewed
• [Journal Article] Specific monoclonal antibody against the nuclear pore complex protein, Nup96. (2010)
  • Author(s): Mizuguchi, C., et al.
  • Journal Title: Hybridoma Volume: 29 Pages: 551-553
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA (2009)
  • Author(s): Katahira, J. and Yoneda, Y
  • Journal Title: RNA Biol. Volume: 6 Pages: 149-152
  • URL: http://dx.doi.org/10.1016/j.bbagrm.2011.12.001
  • Peer Reviewed
• [Journal Article] The role of the nuclear transport system in cell differentiation (2009)
  • Author(s): Yasuhara, N., Oka, M. and Yoneda, Y
  • Journal Title: Semi. Cell Dev. Biol. Volume: 20 Pages: 590-599
  • URL: http://dx.doi.org/10.1016/j.semcdb.2009.05.003
  • Peer Reviewed
• [Journal Article] A high-resolution structure of the pre-microRNA nuclear export machinery (2009)
  • Author(s): Okada, C., Yamashita, E., Lee, S. J., Shibata, S., Katahira, J., Nakagawa, A., Yoneda, Y. and Tsukihara, T
  • Journal Title: Science Volume: 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] Microscopic dissection of the process of stress granule assembly (2009)
  • Author(s): Fujimura, K., Katahira, J., Kano, F., Yoneda, Y. and Murata, M
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research Volume: 1793 Pages: 1728-1737
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor, E47, via heterodimerization (2009)
  • Author(s): Mehmood, R., Yasuhara, N., Oe, S., Nagai, M. and Yoneda, Y
  • Journal Title: Exp. Cell Res. Volume: 315 Pages: 1639-1652
  • URL: http://dx.doi.org/10.1016/j.yexcr.2009.02.025
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies (2009)
  • Author(s): Fujimura, K., Katahira, J., Kano, F., Yoneda, Y. and Murata, M
  • Journal Title: Biochim. Biophys. Acta Volume: 1793 Pages: 878-887
  • URL: http://dx.doi.org/10.1016/j.bbamcr.2009.02.002
  • Peer Reviewed
• [Journal Article] Adaptor Aly and co-adaptor Thoc5function in the Tap-p15-mediated nuclear export of HSP70mRNA (2009)
  • Author(s): Katahira, J., Inoue, H., Hurt, E. and Yoneda, Y
  • Journal Title: EMBO J. Volume: 28 Issue: 5 Pages: 556-567
  • DOI: 10.1038/emboj.2009.5
  • Peer Reviewed
• [Journal Article] mDia2shuttles between the nucleus and the cytoplasm through the Importin-α/β-and CRM1-mediated nuclear transport mechanism (2009)
  • Author(s): Miki, T., Okawa, K., Sekimoto, T., Yoneda, Y., Watanabe, S., Ishizaki, T. and Narumiya, S
  • Journal Title: J. Biol. Chem. Volume: 284 Issue: 9 Pages: 99-104
  • DOI: 10.1074/jbc.m806191200
  • Peer Reviewed
• [Journal Article] Infrared laser-mediated gene induction in targeted single cells in vivo (2009)
  • Author(s): Kamei, Y., Suzuki, M., Watanabe, K., Fujimoto, K., Kawasaki, T., Deguchi, T., Yoneda, Y., Todo, T., Takagi, S., Funatsu, T. and Yuba, S
  • Journal Title: Nature Methods Volume: 6 Pages: 79-81
  • Peer Reviewed
• [Journal Article] Infrared laser-mediated gene induction in targeted single cells in vivo. (2009)
  • Author(s): Kamei, Y., et al.
  • Journal Title: Nature Methods 6 Pages: 79-81
  • Peer Reviewed
• [Journal Article] mDia2 shuttles between the nucleus and the cytoplasm through the Importin-α/β- and CRM1-mediated nuclear transport mechanism. (2009)
  • Author(s): Miki, T., et al.
  • Journal Title: J.Biol. Chem 284 Pages: 99-104
  • Peer Reviewed
• [Journal Article] Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. (2009)
  • Author(s): Katahira, J., et al.
  • Journal Title: EMBO J 28 Pages: 556-567
  • Peer Reviewed
• [Journal Article] Selective localization of PCBP2 to cytoplasmic processing bodies. (2009)
  • Author(s): Fujimura, K., et al.
  • Journal Title: Biochim. Biophys. Acta 1793 Pages: 878-887
  • Peer Reviewed
• [Journal Article] Synergistic nuclear import of NeuroD1 and its partner transcription factor, E47, via heterodimerization. (2009)
  • Author(s): Mehmood, R., et al.
  • Journal Title: Exp.Cell Res 315 Pages: 1639-1652
  • Peer Reviewed
• [Journal Article] Microscopic dissection of the process of stress granule assembly. (2009)
  • Author(s): Fujimura, K., et al
  • Journal Title: Biochim. Biophys. Acta-Molecular Cell Research 1793 Pages: 1728-1737
  • Peer Reviewed
• [Journal Article] A high-resolution structure of the pre-microRNA nuclear export machinery. (2009)
  • Author(s): Okada, C., et al.
  • Journal Title: Science 326 Pages: 1275-1279
  • Peer Reviewed
• [Journal Article] Roles of the TREX complex in nuclear export of mRNA. (2009)
  • Author(s): Katahira, J., Yoneda, Y.
  • Journal Title: RNA Biol Science 6 Pages: 149-152
  • Peer Reviewed
• [Journal Article] Two isoforms of Npap60 (Nup50) differentially regulate nuclear protein import. (2009)
  • Author(s): Ogawa, Y., et al
  • Journal Title: Mol.Biol.Cell 21 Pages: 630-638
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 安原徳子, 岡正啓, 米田悦啓
  • Journal Title: 最新医学3月増刊号「幹細胞研究の最近の進歩」(須田年生監修)(最新医学社)
• [Journal Article] (2009)
  • Author(s): 米田悦啓
  • Journal Title: 実験医学増刊号「細胞核-遺伝情報制御と疾患」(平岡泰、原田昌彦、木村宏、田代聡:編)(羊土社)
• [Journal Article] (2009)
  • Author(s): 米田悦啓
  • Journal Title: 日本医師会雑誌、第138巻・第10号核-細胞質問蛋白質輸送機構の解明と高次生命機能研究への展開(日本医師会)
• [Presentation] 核-細胞質間分子輸送から高次生命機能を見る (2011)
  • Author(s): 米田悦啓
  • Organizer: 第19回臨床医科学フォーラム
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-09-17
• [Presentation] The nucleocytoplasmic transport system regulates the stem cell differentiation (2011)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Yanagida, T., Kaneko, H. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] Role of nucleocytoplasmic shuttling of estrogen receptor (2011)
  • Author(s): Moriyama, T., Oka, M. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] The mechanism to determine a nuclear transport pathway by NLS sequence (2011)
  • Author(s): Kimoto, C., Yasuhara, N. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Importin alpha mediates biased translocation through nuclear pores in conjunction with Nup153 (2011)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] The involvement of small GTPase Ran in cellular aging (2011)
  • Author(s): 永井理博、米田悦啓
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] Nuclear transport machineries and cell function (2011)
  • Author(s): Yoneda, Y.
  • Organizer: 6^<th> Asian-Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila(Philippines)
• [Presentation] The involvement of small GTPase Ran in cellular aging (2011)
  • Author(s): 永井理博、米田悦啓
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
• [Presentation] Importin alphamediates biased translocation through nuclear pores in conjunction withNup153 (2011)
  • Author(s): Ogawa, Y., et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] The mechanism to determine a nuclear transport pathway by NLS sequence (2011)
  • Author(s): Kimoto, C., et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] Nuclear transport machineries and cell function (2011)
  • Author(s): Yoneda, Y.
  • Organizer: 6th Asian-Pacific Organization for Cell Biology Congress
  • Place of Presentation: Manila, Philippines(招待講演)
• [Presentation] 低分子量GTPase Ranが関与する細胞老化制御 (2010)
  • Author(s): 永井理博、安田善也、米田悦啓
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Targeted disruption of one of the importinτfamily members leads to female functional incompetence in delivery (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M. and Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Nuclear import of E47and its partner transcription factors involves across talk between distinct nuclear import pathways (2010)
  • Author(s): Mehmood, R., Yashuhara, N., and Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Nucleo-cytoplasmic shuttling of Oct-4 (2010)
  • Author(s): 岡正啓、盛山哲嗣、浅利宗弘、米田悦啓
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] pre-microRNA核外輸送複合体のX線結晶構造解析 (2010)
  • Author(s): 山下栄樹、慈幸千真理、李守宰、柴田理志、片平じゅん、中川敦史、米田悦啓、月原冨武
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Nuclear protein import machinery and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Nuclear-localized importin alpha regulates gene expression in mammalian spermatogenesis (2010)
  • Author(s): Miyamoto, Y., Yasuda, Y., Yamashiro, T., Nagai, M., Masui, A., Yoneda, Y., Jans, D. A. and Loveland, K.
  • Organizer: OzBio2010. 12^<th> IUBMB and 21^<st> FAOBMB
  • Place of Presentation: Melbourne(Australia)
• [Presentation] Nucleocytoplasmic transport and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: OzBio2010. 12^<th> IUBMB and 21^<st> FAOBMB
  • Place of Presentation: Melbourne(Australia)
• [Presentation] 核-細胞質間蛋白質輸送から見た高次生命機能 (2010)
  • Author(s): 米田悦啓
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] Targeted disruption of the nuclear import adaptor, importinα5, leads to female genital hypoplasia (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M. and Yoneda, Y
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] Transcriptional regulation of importin alpha1 in mouse embryonic stem cells (2010)
  • Author(s): Kamikawa, Y., Yasuhara, N. and Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] The modulation in glycosylation state of nucleoporins in cell differentiation (2010)
  • Author(s): Mizuguchi, C., Yasuhara, N., Asally, M. and Yoneda, Y
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] Differential nuclear import is a novel regulatory mechanism for maintaining pluripotency of ES cells (2010)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Yanagida, T, Kaneko, H. and Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
• [Presentation] 核-細胞質間蛋白質輸送受容体よるES細胞の分化制御機構 (2010)
  • Author(s): 安原徳子、新井由之、Rashid Mehmood、上川泰直、小川泰、柳田敏雄、米田悦啓
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場
• [Presentation] Nucleocytoplasmic protein transport and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: International Symposium on Organelle Network : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: Osaka(招待講演)
• [Presentation] Transcriptional regulation of importin-α1 in embryonic stem cells (2010)
  • Author(s): Kamikawa, Y., Yasuhara, N., Yoneda, Y.
  • Organizer: International Symposium on Organelle Network : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: Osaka
• [Presentation] Targeted disruption of the nuclear import adaptor, importin α5, leads to female genital hypoplasia (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M, Yoneda, Y.
  • Organizer: International Symposium on Organelle Network : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: Osaka
• [Presentation] The role of the nucleocytoplasmic transport system in cell differentiation (2010)
  • Author(s): Yasuhara, N., Yoneda, Y.
  • Organizer: International Symposium on Organelle Network : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: Osaka
• [Presentation] 核一細胞質間分子輸送機構から見た高次生命機能制御 (2010)
  • Author(s): 米田悦啓
  • Organizer: 第2回シグナルネットワーク研究会
  • Place of Presentation: 大阪(招待講演)
• [Presentation] Differential nuclear import is a novel regulatory mechanism for maintaining pluripotency of ES cells (2010)
  • Author(s): Yasuhara, N., Yamagishi, R., Arai, Y., Yanagida, T, Kaneko, H., Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Presentation] The modulation in glycosylation state of nucleoporins in cell differentiation (2010)
  • Author(s): Mizuguchi, C., Yasuhara, N., Asally, M., Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Presentation] Transcriptional regulation of importin alpha1 in mouse embryonic stem cells (2010)
  • Author(s): Kamikawa, Y., Yasuhara, N., Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Presentation] Targeted disruption of the nuclear import adaptor, importin α5 leads to female genital hypoplasia (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M., Yoneda, Y.
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
• [Presentation] 核-細胞質間蛋白質輸送から見た高次生命機能 (2010)
  • Author(s): 米田悦啓
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪(招待講演)
• [Presentation] Nucleocytoplasmic transport and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: OzBio2010, 12th IUBMB and 21st FAOBMB
  • Place of Presentation: Melbourne, Australia(招待講演)
• [Presentation] Nuclear-localized importin alpha regulates gene expression in mammalian spermatogenesis (2010)
  • Author(s): Miyamoto, Y., Yasuda, Y., Yamashiro, T., Nagai, M., Masui, A., Yoneda, Y., Jans, D.A., Loveland, K.
  • Organizer: OzBio2010, 12th IUBMB and 21st FAOBMB
  • Place of Presentation: Melbourne, Australia
• [Presentation] Nucleocytoplasmic transport machineries and cell functions (2010)
  • Author(s): Yoneda, Y.
  • Organizer: Association of Pacific Rim Universities (APRU) Research Symposium on the Interface between Molecular Biology and Nano-Biology
  • Place of Presentation: Kyoto(招待講演)
• [Presentation] Nuclear protein import machinery and cell differentiation (2010)
  • Author(s): Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe(招待講演)
• [Presentation] Nuclear import of E47 and its partner transcription factors involves across talk between distinct nuclear import pathways (2010)
  • Author(s): Mehmood, R., Yashuhara, N., Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery (2010)
  • Author(s): Moriyama, T., Nagai, M., Oka, M., Yoneda, Y.
  • Organizer: BMB2010
  • Place of Presentation: Kobe
• [Presentation] 分化過程におけるKLFファミリータンパク質の核-細胞質間局在の解析 (2009)
  • Author(s): 金子杏美、安原徳子、米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] The modulation in glycosylation state of nucleoporins in cell differentiation (2009)
  • Author(s): 水口千彰、安原徳子、浅利宗弘、米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Nuclear protein import machineries and cell differentiation (2009)
  • Author(s): 米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Molecular dissection of the pathways underlying the nuclear import of NeuroD1, E47 and of their heterodimer (2009)
  • Author(s): Rashid Mehmood、安原徳子、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] A mobile nucleoporin, Nup98, plays an important role in a Crm1-dependent nuclear export (2009)
  • Author(s): 岡正啓、浅利宗弘、安田善也、立花太郎、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] A novel regulatory system to maintain ES cells through the nuclear-cytoplasmic transport (2009)
  • Author(s): 安原徳子、Rashid Mehmood、上川泰直、米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Nuclear protein transport and cell function(Plenary talk) (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 7^<th> Annual Scientific Meeting of ARC Centre of Excellence in Biotechnology and Development
  • Place of Presentation: Kalorama, Victoria(Australia)
• [Presentation] Interaction between importin alpha and Nup153 accelerates nuclear import (2009)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M. and Yoneda, Y.
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff(Canada)
• [Presentation] A novel regulatory mechanism by importin alpha and cell fate determination (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff(Canada)
• [Presentation] Adaptor Aly and Co-Adaptor Thoc5Function in the Tap-p15-Mediated Nuclear Export of HSP70mRNA (2009)
  • Author(s): 片平じゅん、井上仁美、Ed Hurt、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] ペントースリン酸経路代謝酵素Transaldolaseの核内移行の解析 (2009)
  • Author(s): 福本昌宏、辻村賢二、岡正啓、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 細胞分化におけるimportin alpha1の転写制御 (2009)
  • Author(s): 上川泰直、安原徳子、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] importin.とNup153の相互作用は核内輸送を促進する (2009)
  • Author(s): 小川泰、宮本洋一、岡正啓、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Selective Localization of PCBP2to Cytoplamic Processing Bodies (2009)
  • Author(s): 藤村健、片平じゅん、加納ふみ、米田悦啓、村田昌之
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] importin.サブタイプ間の相補性 (2009)
  • Author(s): 安東沙貴子、安原徳子、上川泰直、當間憲一、米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] RanBP1欠損マウスが示す雄性不妊と成長遅滞 (2009)
  • Author(s): 永井理博、盛山哲嗣、Rashid Mehmood、徳弘圭三、伊川正人、岡部勝、田中宏光、米田悦啓
  • Organizer: 第61回日本細胞生物大会
  • Place of Presentation: 名古屋
• [Presentation] 核膜孔を介した物質輸送による核機能制御 (2009)
  • Author(s): 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 神経細胞における核内移行情報伝達分子の検索とその分子機能解析 (2009)
  • Author(s): 福本昌宏、関元敏博、米田悦啓
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
• [Presentation] 核輸送因子による転写因子の選択的輸送機構に関する構造生物学的考察 (2009)
  • Author(s): 山岸良介、安原徳子、米田悦啓、金子寛生
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本
• [Presentation] 核輸送因子Importin.の転写調節機能と精子細胞形成 (2009)
  • Author(s): 宮本洋一、安田善也、山城朋子、永井理博、米田悦啓、David A. Jans、Kate Loveland
  • Organizer: 日本分子生物学会、第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Presentation] 核-細胞質間蛋白質輸送制御と高次生命機能 (2009)
  • Author(s): 米田悦啓
  • Organizer: 日本分子生物学会、第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Presentation] 核-細胞質間蛋白質輸送制御と高次生命機能 (2009)
  • Author(s): 米田悦啓
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Presentation] 核輸送因子Importinαの転写調節機能と精子細胞形成 (2009)
  • Author(s): 宮本洋一, 安田善也, 山城朋子, 永井理博, 米田悦啓, David A.Jans, Kate Loveland
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: 宮崎
• [Presentation] RanBP1欠損マウスが示す雄性不妊と成長遅滞 (2009)
  • Author(s): 永井理博, 盛山哲嗣, Rashid Mehmood, 徳弘圭三, 伊川正人, 岡部勝, 田中宏光, 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] importinαサブタイプ間の相補性 (2009)
  • Author(s): 安東沙貴子, 安原徳子, 上川泰直, 當間憲一, 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Selective Localization of PCBP2 to Cytoplamic Processing Bodies (2009)
  • Author(s): 藤村健, 片平じゅん, 加納ふみ, 米田悦啓, 村田昌之
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] importinαとNup153の相互作用は核内輸送を促進する (2009)
  • Author(s): 小川泰, 宮本洋一, 岡正啓, 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 細胞分化におけるimportin alphalの転写制御 (2009)
  • Author(s): 上川泰直, 安原徳子, 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] Adaptor Aly and Co-Adaptor Thoc5 Function in the Tap-p15-Mediated Nuclear Export of HSP70 mRNA (2009)
  • Author(s): 片平じゅん, 井上仁美, Ed Hurt, 米田悦啓
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] A novel regulatory mechanism by importin alpha and cell fate determination. (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff, Canada
• [Presentation] Interaction between importin alpha and Nup153 accelerates nuclear import (2009)
  • Author(s): Ogawa, Y., Miyamoto, Y., Oka, M., Yoneda, Y
  • Organizer: 2009 International Meeting on Nuclear Trafficking
  • Place of Presentation: Banff, Canada
• [Presentation] Nuclear protein transport and cell function. (Plenary talk) (2009)
  • Author(s): Yoneda, Y.
  • Organizer: 7^<th> Annual Scientific Meeting of ARC Centre of Excellence in Biotechnology and Development
  • Place of Presentation: Kalorama, Victoria, Australia
• [Presentation] A mobile nucleoporin, Nup98, plays an important role in a Crml-dependent nuclear export (2009)
  • Author(s): 岡正啓, 浅利宗弘, 安田善也, 立花太郎, 米田悦啓
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] ストレスにより核内集積したimportin αによる転写制御 (2009)
  • Author(s): 桝井あゆみ, 安田善也, 宮本洋一, 水口千彰, 米田悦啓
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/
• [Remarks]
  • URL: http://www.anat3.med.osaka-u.ac.jp/