embryonic patterning by Nodal and Lefty

• Project/Area Number: 21247034
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Osaka University
• Principal Investigator: HAMADA Hiroshi 大阪大学, 生命機能研究科, 教授 (00208589)
• Co-Investigator(Kenkyū-buntansha): SHIRATORI Hidetaka 大阪大学, 生命機能研究科, 准教授 (90362590)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000); Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 胚発生 / Nodal シグナル / Lefty / 胚葉形成 / 体軸形成 / Nodalシグナル / 形熊形成 / シグナル因子 / 左右非対称性

Research Abstract

1. We have clarified the origin and role of DVE in anterior-posterior patterning.
2. We have identified a number of FoxH1 target genes in the mouse embryo.
3. A retinoic acid-responsive enhancer regulates Nodal expression at the pre-gastrulation stage.
4. Cerl2 is the earliest responding gene to the nodal fluid flow.

Research Products

• [Journal Article] Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA in the mouse embryo (2012)
  • Author(s): Nakamura, T., Saito, D., Kawasumi, A., Shinohara, K., Asai, Y., Takaoka, K., Dong, F., Takamatsu, A., Belo, J.A., Mochizuki, A., and Hamada, H.
  • Journal Title: Nat.Communic Volume: 3 Issue: 1 Pages: 1322-1322
  • DOI: 10.1038/ncomms2319
  • Peer Reviewed
• [Journal Article] Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2 (2012)
  • Author(s): Yoshiba, S., Shiratori, H., Kawasumi, A., Shinohara, K., Sasaki, G., Belo, J.A., Nonaka, S., Sasaki, H., Kuo, I., Ehrlich, B., Pennekamp, P., Dworniczak, B., and Hamada, H
  • Journal Title: Science. Volume: 338 Pages: 226-231
  • Peer Reviewed
• [Journal Article] Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo. (2012)
  • Author(s): Shinohara, K.,* Kawasumi, A.,* Takamatsu, A., Yoshiba, S., Botilde, Y., Motoyama, N., Reith, W., Durand, B., Shiratori, H. and Hamada, H.
  • Journal Title: (*equally contributed) Nature Commun Volume: 3 Pages: 622-622
  • Peer Reviewed
• [Journal Article] Cell specification and axis formation in the mouse embryo (review). (2012)
  • Author(s): Takaoka, K. and Hamada, H.
  • Journal Title: Development. Volume: 139 Pages: 3-14
  • Peer Reviewed
• [Journal Article] Cell specification and axis formation in the mouse embryo (2012)
  • Author(s): Katsuyoshi Takaoka
  • Journal Title: Development Volume: 139 Issue: 1 Pages: 3-14
  • DOI: 10.1242/dev.060095
  • Peer Reviewed
• [Journal Article] Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo (2012)
  • Author(s): Kyosuke Shinohara
  • Journal Title: Nature Commun. Volume: 3 Issue: 1 Pages: 622-622
  • DOI: 10.1038/ncomms1624
  • Peer Reviewed
• [Journal Article] Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo. (2011)
  • Author(s): Takaoka, K., Yamamoto, M. and Hamada, H.
  • Journal Title: Nat. Cell Biol. Volume: 13(7) Pages: 743-752
• [Journal Article] Left-right asymmetry in the level of active Nodal protein produced in the node is translated into the asymmetry in the lateral plate of mouse embryo. (2011)
  • Author(s): Kawasumi, A., Nakamura, T., Iwai, N., Yashiro, K., Saijoh, Y., Belo, J.A., Shiratori, H. and Hamada, H
  • Journal Title: Dev Biol. Volume: 353 Pages: 321-330
  • Peer Reviewed
• [Journal Article] Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo (2011)
  • Author(s): Katsuyoshi Takaoka
  • Journal Title: Nat. Cell Biol. Volume: 13 Issue: 7 Pages: 743-752
  • DOI: 10.1038/ncb2251
  • Peer Reviewed
• [Journal Article] Left-right asymmetry in the level of active Nodal protein produced in the node is translated into the asymmetry in the lateral plate of mouse embryo (2011)
  • Author(s): Aiko Kawasumi
  • Journal Title: Developmental Biology Volume: 353 Issue: 2 Pages: 321-330
  • DOI: 10.1016/j.ydbio.2011.03.009
  • Peer Reviewed
• [Journal Article] Wnt signaling escapes to cilia (2011)
  • Author(s): Reiko Ajima
  • Journal Title: Nat. Cell Biol. Volume: 13 Issue: 6 Pages: 636-637
  • DOI: 10.1038/ncb0611-636
• [Journal Article] Loss of Cited2 causes congenital heart defects by perturbing left-right patterning of the body axis. (2011)
  • Author(s): Floro, K.L., Artap, S.T., Preis, J.I., Sparrow, D.B., Fatkin, D., Chapman, G., Furtado, M.B., Harvey, R.P., Hamada, H., Sparrow, D.B., Dunwoodie, S.L.
  • Journal Title: Human Mol.Genetics. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Left-right asymmetry in the level of active Nodal protein produced in the node is translated into the asymmetry in the lateral plate of mouse embryo. (2011)
  • Author(s): Kawasumi, A., Nakamura, T., Iwai, N., Yashiro, K., Saijoh, Y., Belo, J.A., Shiratori, H., Hamada, H.
  • Journal Title: Dev Biol. Volume: (In press)
  • Peer Reviewed
• [Journal Article] Translation of anterior-posterior information into left-right polarity. (2010)
  • Author(s): Hashimoto, M. and Hamada, H.
  • Journal Title: Curr. Opin. Genet. Dev Volume: 20 Pages: 433-437
  • Peer Reviewed
• [Journal Article] Planar polarization of the node cells determines the rotation axis of the node cilia (2010)
  • Author(s): Hashimoto, M., Shinohara, K., Wang, J., Ikeuchi, S., Meno, C., Yoshiba, S., Takada S., Hatta, K., Wynshaw-Boris, T.,and Hamada, H.
  • Journal Title: Nat. Cell Biol Volume: 12 Pages: 170-176
  • Peer Reviewed
• [Journal Article] Translation of anterior-posterior information into left-right polarity. (2010)
  • Author(s): Hashimoto, M., Hamada, H.
  • Journal Title: Curr.Opin.Genet.Dev. Volume: 20 Pages: 433-437
  • Peer Reviewed
• [Journal Article] Reversal of left-right asymmetry by aberrant Nodal signaling in the node of mouse embryos. (2009)
  • Author(s): Oki, S., Kitajima, K., Belo, J.A., Yokoyama, T., Hamada, H.* and Meno, C.*
  • Journal Title: (*corresponding authors). Development Volume: 136 Pages: 3917-3925
  • Peer Reviewed
• [Journal Article] Removal of maternal retinoic acid by embryonic CYP26 for correct Nodal regulation during early embryonic patterning. (2009)
  • Author(s): Uehara, M., Yashiro, K., Takaoka, K., Yamamoto M., and Hamada, H.
  • Journal Title: Genes & Dev. Volume: 23 Pages: 1689-1698
  • Peer Reviewed
• [Journal Article] Antagonism between Smad1 and Smad2 signaling regulates formation of the distal visceral endoderm in the mouse embryo (2009)
  • Author(s): Yamamoto M., Beppu, X., Takaoka, K., Meno, C., Li, E., Miyazono, K., and Hamada, H
  • Journal Title: J. CellBiol Volume: 184 Pages: 323-334
  • Peer Reviewed
• [Journal Article] Removal of maternal retinoic acid by embryonic CYP26 for correct Nodal regulation during early embryonic patterning (2009)
  • Author(s): Uehara, M., ら5名
  • Journal Title: Genes & Dev. 23 Pages: 1689-1698
  • Peer Reviewed
• [Journal Article] Reversal of left-right asymmetry by aberrant Nodal signaling in the node of mouse embryos. (2009)
  • Author(s): Oki, S., ら6名
  • Journal Title: Development 136 Pages: 3917-3925
  • Peer Reviewed
• [Presentation] Asymmetric Flow is Converted to a Degradation of Cerl2and Amplified by Interlinked Feedbacks Robustly in a Left-Right Axis of the Mouse Embryo (2012)
  • Author(s): Tetsuya Nakamura, Daisuke Saitoh, Kyosuke Shinohara, Dong Felang, Aiko Kawasumi, Atsuko Takamatsu,Jose Antonio Belo, Atsushi Mochizuki, Hiroshi Hamada.
  • Organizer: CDB Symposium; Quantitative Biology
  • Place of Presentation: RIKEN Kobe
  • Year and Date: 2012-02-26
• [Presentation] 弱い水の流れを大きな左右非対称性に変換するしくみ (2012)
  • Author(s): 中村哲也、川住愛子、Owen Tampilin, 斉藤大助、篠原恭介、高松敦子、望月敦史、濱田博司
  • Organizer: 定量生物学の会第4回年会
  • Place of Presentation: 名古屋大学￥
• [Presentation] 哺乳類が高い Canalization を達成する機構 (2011)
  • Author(s): 中村哲也、斉藤大助、Owen Tampilin、川住愛子、篠原恭介、高松敦子、望月敦史、濱田博司
  • Organizer: GSC 七夕ミーティング2011
  • Place of Presentation: 横浜理化学研究所
  • Year and Date: 2011-08-25
• [Presentation] Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo (2011)
  • Author(s): Katsuyoshi Takaoka, Masamichi Yamamoto, Hiroshi Hamada
  • Organizer: 第44回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] ECLP regulates cilia formation and cilia-related signals in mouse embryo (2011)
  • Author(s): Botilde Yanick, 吉場聡子 , 濱田博司
  • Organizer: 第44回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Polycystin-2 mediates flow-derived signal to establish left-right asymmetry at the edge of the node (2011)
  • Author(s): 吉場聡子, 白鳥秀卓、川住愛子、篠原恭介、Jose Belo、中井淳一、野中茂紀、佐々木洋、 Ivana Kuo, Barbara Ehrlich、Petra Pennekamp、 Bernd Dworniczak、濱田博司
  • Organizer: 第44回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Left-right asymmetry in Smad2/3 phosphorylation in the node of mouse embryo is translated into (correlates with) asymmetry in the lateral plate (2011)
  • Author(s): 川住愛子、中村哲也、岩井なおみ、八代健太、西條幸男、 Jose Belo、白鳥秀卓、濱 田 博 司
  • Organizer: 第44回発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Sensing fluid flow for left-right patterning (2011)
  • Author(s): Hamada Hiroshi
  • Organizer: Cellular Development: Biology at the Interface
  • Place of Presentation: RIKEN Center for Developmental Biology(兵庫県)
  • Invited
• [Presentation] Genetics of left-right asymmetry in mammals (2011)
  • Author(s): Hamada Hiroshi
  • Organizer: The 12th International Congress of Human Genetics
  • Place of Presentation: Montreal Convention Center(Canada)
  • Invited
• [Presentation] 'Origin of Anterior-Posterior axis in the mouse embryos' From Oocyte to Embryo (2010)
  • Author(s): Katsuyoshi Takaoka, Hiroshi Hamada
  • Organizer: Workshop on Early Mammalian Embryogenesis
  • Place of Presentation: Warsaw,Poland
• [Presentation] マウス胚における前後軸の起源 (2010)
  • Author(s): 高岡勝吉、山本正道、濱田博司
  • Organizer: 日本発生生物学会第43回大会
  • Place of Presentation: 京都
• [Presentation] マウス初期胚ノード繊毛の可視化計測 (2009)
  • Author(s): 篠原恭介,高松敦子,橋本昌和,吉場聡子,川住愛子,濱田博司
  • Organizer: 日本機械学会 流体工学部門会
  • Place of Presentation: 名古屋工業大学
• [Presentation] Mechanisms of left-right asymmetric signal generation around the node (2009)
  • Author(s): Aiko Kawasumi, Naomi Iwai, Jose Antonio Belo, Tetsuya Nakamura, Hidetaka Shiratori, Hiroshi Hamada
  • Organizer: The 16th International Society of Developmental Biologists Congress.
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Cell polarity in the node for basal body positioning and nodal flow (2009)
  • Author(s): Masakazu Hashimoto, Shingo Ikeuchi, Kyosuke Shinohara, Shigenori Nonaka, Anthony Wynshaw-Boris, and Hiroshi Hamada.
  • Organizer: The 16th International Society of Developmental Biologists Congress.
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] マウス胚における前後軸の起源 (2009)
  • Author(s): 高岡勝吉、山本正道、濱田博司
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟トキメッセ
• [Remarks] ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hamada/