Budget Amount *help |
¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000)
Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2010: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2009: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
|
Research Abstract |
We cloned pnd-1 and-2 genes that have been known as genes initiating Bombyx mori embryonic diapause, using both procedures of positional cloning and RNA interference. We also cloned a strong candidate gene connected the diapause hormone signal to the expression of pnd genes, using DNA microarray analysis. We isolated a gene for heat shock transcription factor(HSF) as a factor transducing the 5℃signal. HSF was shown to bind to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities.
|