Molecular cloning and characterization of genes responsible for the diapause-initiation and the diapause-termination induced by cold in Bombyx mori eggs

• Project/Area Number: 21248007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Nagoya University
• Principal Investigator: YAGINUMA Toshinobu 名古屋大学, 生命農学研究科, 教授 (60135332)
• Co-Investigator(Kenkyū-buntansha): NIIMI Teruyuki 名古屋大学, 大学院・生命農学研究科, 准教授 (00293712) ; IKEDA Motoko 名古屋大学, 大学院・生命農学研究科, 准教授 (20262892) ; KADONO Keiko (独)農業生物資源研究所, 主任研究員 (40355722) ; SHIOMI Kunihiro 信州大学, 繊維学部, 准教授 (70324241)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000); Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2010: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000); Fiscal Year 2009: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
• Keywords: カイコ / 胚休眠 / 休眠開始遺伝子 / 休眠覚醒 / 低温誘導性遺伝子 / 低温シグナル受容・伝達 / 低温シグナル / 低温シグナル伝達 / 低温シグナル受容 / 低温誘導遺伝子

Research Abstract

We cloned pnd-1 and-2 genes that have been known as genes initiating Bombyx mori embryonic diapause, using both procedures of positional cloning and RNA interference. We also cloned a strong candidate gene connected the diapause hormone signal to the expression of pnd genes, using DNA microarray analysis. We isolated a gene for heat shock transcription factor(HSF) as a factor transducing the 5℃signal. HSF was shown to bind to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities.

Research Products

• [Journal Article] Trehalase-2 protein contributes to trehalase activity enhanced by diapause hormone in developing ovaries of the silkworm (2011)
  • Author(s): Kamei, Y., Hasegawa, Y., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. J. Insect Physiol Volume: 57(5) Pages: 608-613
  • Peer Reviewed
• [Journal Article] Cloning of cDNAs encoding sorbitol dehydrogenase-2a and b, enzymatic characterization and up-regulated expression of the genes in Bombyx mori diapause eggs exposed to 5℃ (2011)
  • Author(s): Rubio, R. O., Suzuki, A., Mitsumasu, K., Homma, T., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Insect Biochem. Mol. Biol Volume: 41(6) Pages: 378-387
  • Peer Reviewed
• [Journal Article] Heat shock factor binds to heat shock elements at 5'-upstreams of heat shock protein 70a and Samui genes to confer their transcription activities in Bombyx mori diapause eggs exposed to 5℃ (2011)
  • Author(s): Kihara, F., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Insect Biochem. Mol. Biol Volume: 41(11) Pages: 843-851
  • Peer Reviewed
• [Journal Article] trehalase-2 protein contributes to trehalase activity enhanced by diapause hormone in developing ovaries of the silkworm, Bombyx mori (2011)
  • Author(s): Kamei, Y., Hasegawa, Y., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: Journal of Insect Physiology Volume: 57 Issue: 5 Pages: 608-613
  • DOI: 10.1016/j.jinsphys.2010.10.001
  • Peer Reviewed
• [Journal Article] Cloning of cDNAs encoding sorbitol dehydrogenase-2a and b, enzimiatic characterization and up-regulated expression of the genes in Bombyx moridiapause eggs exposed to 5℃ (2011)
  • Author(s): Rubio, R.O., Suzuki, A., Mitsumasu, K., Homma, T., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 41 Issue: 6 Pages: 378-387
  • DOI: 10.1016/j.ibmb.2011.02.007
  • Peer Reviewed
• [Journal Article] Heat shock factor binds to heat shock elements at 5'-upstreams of heat shockprotein 70a and Samui genes to confer their transcription activities in Bombyxmori diapause eggs exposed to 5℃ (2011)
  • Author(s): Kihara, F., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 41 Issue: 11 Pages: 843-851
  • DOI: 10.1016/j.ibmb.2011.06.006
  • Peer Reviewed
• [Journal Article] Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs (2010)
  • Author(s): Moribe, Y., Oka, K., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: J. Insect Physiol Volume: 56 Pages: 1246-1252
  • Peer Reviewed
• [Journal Article] Disappearance of chorion proteins in Bombyx mori eggs treated with HCl solution to prevent diapause (2010)
  • Author(s): Tsurumaru, S., Kawamori, A., Mitsumasu, K., Niimi, T., Imai, K., Yamashita, O. and Yaginuma, T
  • Journal Title: J. Insect Physiol Volume: 56 Pages: 1721-1727
  • Peer Reviewed
• [Journal Article] Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs (2010)
  • Author(s): Moribe, Y., Oka, K., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: Journal of Insect Physiology Volume: 56 Pages: 1246-1252
  • Peer Reviewed
• [Journal Article] Disappearance of chorion proteins in Bombyx mori eggs treated with HCl solution to prevent diapause (2010)
  • Author(s): Tsurumaru, S., Kawamori, A., Mitsumasu, K., Niimi' T., Imai, K., Yamashita, O., Yaginuma' T.
  • Journal Title: Journal of Insect Physiology Volume: 56 Pages: 1721-1727
  • Peer Reviewed
• [Journal Article] Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs (2010)
  • Author(s): Moribe, Y., Oka, K., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: J.Insect Physiol. (In press)
  • Peer Reviewed
• [Journal Article] Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori (2009)
  • Author(s): Ohta, H., Tsuchihara, K., Mitsumasu, K., Yaginuma, T., Ozoe, Y. and Asaoka, K
  • Journal Title: Insect Biochem. Mol. Biol Volume: 39 Pages: 342-347
  • Peer Reviewed
• [Journal Article] Novel gene encoding precursor protein consisting of possible several neuropeptides expressed in brain and frontal ganglion of the silkworm (2009)
  • Author(s): Mitsumasu, K., Tanaka, Y., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. Peptides Volume: 30 Pages: 1233-1240
  • Peer Reviewed
• [Journal Article] Glycerol kinase activity and glycerol kinase-3 gene are up-regulated by acclimation to 5℃in diapause eggs of the silkworm (2009)
  • Author(s): Kihara, F., Itoh, K., Iwasaka, M., Niimi, T., Yamashita, O. and Yaginuma, T
  • Journal Title: Bombyx mori. Insect Biochem. Mol. Biol Volume: 39 Pages: 763-769
  • Peer Reviewed
• [Journal Article] Glycerol kinase activity and glycerol kinase-3 gene are up-regulated by acclimation to 5℃ in diapause eggs of the silkworm, Bombyx mori (2009)
  • Author(s): Kihara, F., Itoh, K., Iwasaka, M., Niimi, T., Yamashita, O., Yaginuma, T.
  • Journal Title: Insect Biochem.Mol.Biol. 39 Pages: 763-739
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 柳沼利信(分担執筆)
  • Journal Title: カイコ胚休眠の生化学・分子生物学.「虫たちが語る生物学の未来」(財団法人衣笠会編)(財団法人衣笠会) Pages: 146-153
• [Presentation] カイコ卵休眠遺伝子pnd,pnd-2の単離と解析 (2012)
  • Author(s): 城所久良子・新美輝幸・柳沼利信, 他6名
  • Organizer: 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2012-03-19
• [Presentation] カイコ完成卵において食道下神経節が抑制する遺伝子の単離と発現解析 (2011)
  • Author(s): 亀井勇樹・鈴木亜希子・新美輝幸・柳沼利信
  • Organizer: 日本蚕糸学会東海支部講演会
  • Place of Presentation: セラヴィリゾート泉郷(山梨県)
  • Year and Date: 2011-11-24
• [Presentation] カイコ胚休眠誘導に関わる遺伝子の探索:食道下神経節の有無に依存して発現する遺伝子の単離 (2011)
  • Author(s): 鈴木亜季子, ら
  • Organizer: 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 蚕糸・昆虫機能利用学術講演会要旨集
  • Year and Date: 2011-03-21
• [Presentation] Bombyx mori embryonic diapause : transducers of diapause hormone signal for diapause initiation and 5℃signal for diapause termination (2011)
  • Author(s): Yaginuma, T., Suzuki, A., Kihara, F., Kamei, Y., Niimi, T., Yamashita, O
  • Organizer: The Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: アムステルダム
• [Presentation] Bombyx mori embryonic diapause : transducers of diapause hormone signal for diapause initiation and 5℃ signal for diapause termination (2011)
  • Author(s): Yaginuma, T., Suzuki, A., Kihara, F., Kamei, Y., Niimi, T., Yamashita, O.
  • Organizer: Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: アムステルダム(オランダ)
• [Presentation] 温暖化と昆虫-特に休眠の観点から- (2010)
  • Author(s): 柳沼利信
  • Organizer: 名古屋大学大学院生命農学研究科附属フィールド科学教育研究センター東郷フィールド講演会
  • Place of Presentation: 東郷町
  • Year and Date: 2010-06-26
• [Presentation] 形質と環境-カイコ胚休眠の観点から- (2009)
  • Author(s): 柳沼利信
  • Organizer: 日大理学部自然科学研究所シンポジウム「動物の不思議を科学する」
  • Place of Presentation: 日本大学
  • Year and Date: 2009-06-20
• [Presentation] 形質と環境-カイコ胚休眠の観点から- (2009)
  • Author(s): 柳沼利信
  • Organizer: 日大文理学部総合文化研究室/自然科学研究所/世田谷区教育委員会シンポジウム「動物の不思議を科学する」
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2009-06-20
• [Book] 休眠ホルモンによるカイコガ胚休眠の調節脱皮・変態の生物学-昆虫と甲殻類のホルモン作用の謎を追う-(園部治之・長澤寛道編) (2011)
  • Author(s): 柳沼利信,新美輝幸,塩見邦博
  • Publisher: 東海大学出版会
• [Book] 休眠ホルモンによるカイコガ胚休眠の調節.脱皮・変態の生物学-昆虫と甲殻類のホルモン作用の謎を追う-(園部治之・長澤寛道編) (2011)
  • Author(s): 柳沼利信・新美輝幸・塩見邦博(分担執筆)
  • Publisher: 東海大学出版会
• [Book] 低温誘導性遺伝子Samui.昆虫の低温耐性-その仕組みと調べ方-(積木久明・田中一裕・後藤三千代編) (2010)
  • Author(s): 柳沼利信
  • Publisher: 岡山大学出版会
• [Book] 昆虫の低温耐性-その仕組みと調べ方-(積木久明・田中一裕・後藤三千代編)第8部 第5章 低温誘導性遺伝子Samui (2010)
  • Author(s): 柳沼利信(分担執筆)
  • Publisher: 岡山大学出版会
• [Book] カイコ胚休眠の生化学・分子生物学虫たちが語る生物学の未来(衣笠会編) (2009)
  • Author(s): 柳沼利信
  • Publisher: (財団法人)衣笠会
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/.yousan/
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~yousan/