Identification of novel transporters involved in toxic metals

• Project/Area Number: 21248009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: MA Jian feng 岡山大学, 資源植物科学研究所, 教授 (80260389)
• Co-Investigator(Kenkyū-buntansha): YAMAJI Naoki 岡山大学, 資源植物科学研究所, 助教 (00444646)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000); Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2009: ¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
• Keywords: トランスポーター / ヒ素 / カドミウム / アルミニウム / 有害金属

Research Abstract

We identified several transporter genes involved in accumulation and detoxification of toxic metals including Cd, As and Al. TcHMA3 isolated from a Cd hyperaccumulator is required for tolerating high Cd and OsHMA3 from rice is involved in Cd accumulation in grain. We also found that Lsi1, a silicon influx transporter, is able to transport methylated arsenic(DMA and MMA). Furthermore, we have identified several novel transporter genes involved in Al tolerance in rice, Arabidopsis and buckwheat.

Research Products

• [Journal Article] Acquisition of aluminum tolerance by modification of a single gene in barley (2012)
  • Author(s): Fujii M., Yokosho K., Yamaji N., Saisho D., Yamane M., Takahashi H., Sato K., Nakazono M., Ma J.F.
  • Journal Title: Nature Communications Volume: Vol.3 Issue: 1 Pages: 713-713
  • DOI: 10.1038/ncomms1726
  • Peer Reviewed
• [Journal Article] A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots (2012)
  • Author(s): Huang, C. F., Yamaji, N., Ono, K., Ma, J. F.
  • Journal Title: Plant Journal Volume: Vol.69 Pages: 565-576
  • Peer Reviewed
• [Journal Article] A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice (2012)
  • Author(s): Huang, C. F., Yamaji, N., Chen, Z. and Ma, J. F.
  • Journal Title: Plant Journal Volume: Vol.69 Pages: 857-867
  • Peer Reviewed
• [Journal Article] A leucine-rich repeat receptor-like kinase gene is involved in the specification of outer cell layers in rice roots (2012)
  • Author(s): Huang, C.F., Yamaji, N., Ono, K., Ma, J.F.
  • Journal Title: Plant J. Volume: 69 Issue: 4 Pages: 565-576
  • DOI: 10.1111/j.1365-313x.2011.04824.x
  • Peer Reviewed
• [Journal Article] A tonoplast-localized half-size ABC transporter is required for internal detoxificati on of aluminum in rice (2012)
  • Author(s): Huang, C.F., Yamaji, N., Chen, Z., Ma, J.F.
  • Journal Title: Plant J. Volume: 69 Issue: 5 Pages: 857-867
  • DOI: 10.1111/j.1365-313x.2011.04837.x
  • Peer Reviewed
• [Journal Article] An Al-inducible MATE gene is involved in external detoxification of Al in rice (2011)
  • Author(s): Yokosho, K., Yamaji, N., Ma, J. F.
  • Journal Title: Plant Journal Volume: Vol.68 Pages: 1061-1069
  • Peer Reviewed
• [Journal Article] High resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots (2011)
  • Author(s): Moore, K. L., Schroder, M., Wu, Z. C., Martin, B. G. H., Hawes, C. R., Mcgrath, S. P., Hawkesford, M. J., Ma, J. F., Zhao, F. J. Grovenor, C. R. M.
  • Journal Title: Plant Physiology Volume: Vol.156 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Further characterization of an aluminum influx transporter in rice (2011)
  • Author(s): Xia, J. X., Yamaji, N., Ma, J. F.
  • Journal Title: Plant Signal. Behavior Volume: Vol.6 Pages: 160-163
  • Peer Reviewed
• [Journal Article] Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens (2011)
  • Author(s): Ueno, D., Milner, M., Yamaji, N., Yokosho, K., Koyama, E., Zambrano, C., Kaskie, M., Ebbs, S., Kochian, L., Ma, J. F.
  • Journal Title: Plant Journal Volume: Vol.66 Pages: 852-862
  • Peer Reviewed
• [Journal Article] Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar (2011)
  • Author(s): Ueno, D., Koyama, E., Yamaji, N., Ma, J. F.
  • Journal Title: Jarjan. J., Expt. Bot. Volume: Vol.62 Pages: 2265-2272
  • Peer Reviewed
• [Journal Article] High resolution secondary ion mass spectrometry reveals the contrasting subcellular distribution of arsenic and silicon in rice roots (2011)
  • Author(s): Moore, K.L., Schroder, M., Wu, Z.C., Martin, B.G.H., Hawes, C.R., Mograth, S.P., Hawkesford, M.J., Ma, J.F., Zhao, FJ., Grovenor, C.R.M.
  • Journal Title: Plant Physiol. Volume: 156 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens (2011)
  • Author(s): Ueno, D., Milner, M., Yamaji, N., Yokosho, K., Koyama, E., Zambrano, C., Kaskie, M., Ebbs, S., Kochian, L., Ma, J.F.
  • Journal Title: Plant J. Volume: 66 Pages: 852-862
  • Peer Reviewed
• [Journal Article] Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan (2011)
  • Author(s): Ueno, D., Koyama, E., Yamaji, N., Ma, J.F.
  • Journal Title: J.Expt.Bot. Volume: 62 Pages: 2265-2272
  • Peer Reviewed
• [Journal Article] Gene limiting cadmium accumulation in rice. (2010)
  • Author(s): Ueno, D, Yamaji, N., Kono, I., Huang, C. F., Ando, T., Yano, M., Ma, J. F.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: Vol.107 Issue: 38 Pages: 16500-16505
  • DOI: 10.1073/pnas.1005396107
  • Peer Reviewed
• [Journal Article] A plasma membrane-localized transporter for aluminum in rice (2010)
  • Author(s): Xia, J. X., Yamaji, N., Ma, J. F.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: Vol.107 Issue: 43 Pages: 18381-18385
  • DOI: 10.1073/pnas.1004949107
  • Peer Reviewed
• [Journal Article] Knockout of a bacterial-type ABC transporter gene, AtSTAR1, results in increased Al sensitivity in Arabidopsis (2010)
  • Author(s): Huang, C. F., Yamaji, N., Ma, J. F.
  • Journal Title: Plant Physiol. Volume: Vol.153 Pages: 1669-1677
  • Peer Reviewed
• [Journal Article] The role of the rice aquaporin Lsi1 in arsenite efflux from roots (2010)
  • Author(s): Zhao, F. J., Ago, Y., Mitani, N., Li, R. Y., Su, Y. H., Yamaji, N., McGrath, S. P., Ma, J. F.
  • Journal Title: New Phytol. Volume: Vol.186 Pages: 392-399
  • Peer Reviewed
• [Journal Article] Isolation and characterisation of two MATE genes in rye (2010)
  • Author(s): Yokosho, K., Yamaji, N., Ma, J. F.
  • Journal Title: Funct. Plant Biol. Volume: Vol.37 Pages: 296-303
  • Peer Reviewed
• [Journal Article] Isolation and characterisation of two MATE genes in rye. (2010)
  • Author(s): Yokosho, K., Yamaji, N., Ma, J.F.
  • Journal Title: Funct, Plant Biol. Volume: 37 Pages: 296-303
  • Peer Reviewed
• [Journal Article] The role of the rice aquaporin Lsi1 in arsenite efflux from roots (2010)
  • Author(s): Zhao, F.J., Ago, Y., Mitani, N., Li, R.Y., Su, Y.H., Yamaji, N., McGrath, S.P., Ma, J.F.
  • Journal Title: New Phytol. Volume: 186 Pages: 392-399
  • Peer Reviewed
• [Journal Article] Knockout of a bacterial-type ABC transporter gene, AtSTAR1, results in increased Al sensitivity in Arabidopsis (2010)
  • Author(s): Huang, C.F., Yamaji, N., Ma, J.F., 2010
  • Journal Title: Plant Physiol. Volume: 153 Pages: 1669-1671
  • Peer Reviewed
• [Journal Article] A plasma membrane-localized transporter for aluminum in rice (2010)
  • Author(s): Xia, J.X., Yamaji, N., Ma, J.F.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 18381-18385
  • Peer Reviewed
• [Journal Article] Gene limiting cadmium accumulation in rice (2010)
  • Author(s): Ueno, D.Yamaji, N.Kono, I., Huang, C.F., Ando, T., Yano, M., Ma, J.F.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 16500-16505
  • Peer Reviewed
• [Journal Article] A major quantitative trait locus controlling cadmium translocation in rice(Oryza sativa) (2009)
  • Author(s): Ueno, D., Kono, I., Yokosho, K., Ando, T., Yano, M., Ma, J. F.
  • Journal Title: New Phytologist Volume: 182 Pages: 644-653
  • Peer Reviewed
• [Journal Article] Arsenic uptake and metabolism in plants (2009)
  • Author(s): Zhao, F. J., Ma, J. F., Meharg, A. A., McGrath, S. P.
  • Journal Title: New Phytol. Volume: Vol.181 Pages: 777-794
  • Peer Reviewed
• [Journal Article] Mitigation of arsenic accumulation in rice with water management and silicon fertilization (2009)
  • Author(s): Li, R. Y., Stroud, J. L., Ma, J. F., McGrath, S. P., Zhao, F. J.
  • Journal Title: Environ. Sci. Technol. Volume: Vol.43 Pages: 3778-3783
  • Peer Reviewed
• [Journal Article] The rice aquaporin Lsi1 mediates uptake of methylated arsenic species (2009)
  • Author(s): Li, R. Y., Ago, Y., Liu, W. J., Mitani, N., Feldmann, J., McGrath, S. P., Ma, J. F., Zhao, F. J.
  • Journal Title: Plant Physiol. Volume: Vol.150 Pages: 2071-2080
  • Peer Reviewed
• [Journal Article] Mitigation of arsenic accumulation in rice with water management and silicon fertilization (2009)
  • Author(s): Li, R.Y., Stroud, J.L., Ma, J.F., McGrath, S.P., Zhao, F.J.
  • Journal Title: Environ. Sci. Technol. 43 Pages: 3778-3783
  • Peer Reviewed
• [Journal Article] The rice aquaporin Lsil mediates uptake of methylated arsenic species (2009)
  • Author(s): (3) Li, R.Y., Ago, Y., Liu, W.J., Mitani, N., Feldmann, J., McGrath, S.P., Ma, J.F., Zhao, F.J.
  • Journal Title: Plant Physiol. 150 Pages: 2071-2080
  • Peer Reviewed
• [Journal Article] Identification of a novel major quantitative trait locus controlling distribution of Cd between roots and shoots in rice (2009)
  • Author(s): (1) Ueno, D., Koyama, E., Kono, I., Ando, T., Yano, M., Ma, J.F.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2223-2233
  • Peer Reviewed
• [Presentation] 作物の生産と品質に貢献するミネラルトランスポーター研究 (2011)
  • Author(s): 馬建鋒
  • Organizer: 植物微生物研究会第21回研究交流会プログラム
  • Place of Presentation: 岡山(招待講演)
  • Year and Date: 2011-09-20
• [Presentation] ケイ酸トランスポーターLsi1は亜ヒ酸も亜セレン酸も輸送する (2011)
  • Author(s): 馬建鋒、山地直樹、三谷奈見季
  • Organizer: 第3回植物アクアポリン研究検討会
  • Place of Presentation: 倉敷
  • Year and Date: 2011-06-03
• [Presentation] イネケイ酸輸送体OsNIP2;1の基質選択性の解析 (2011)
  • Author(s): 三谷奈見季・山地直樹・Zhao, F-J.・馬建鋒
  • Organizer: 第3回植物アクアポリン研究検討会
  • Place of Presentation: 倉敷
  • Year and Date: 2011-06-03
• [Presentation] イネヒ素とカドミウム吸収の分子機構 (2011)
  • Author(s): 馬建鋒
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 2011-03-29
• [Presentation] Genes involved in the accumulation of toxic Cd and As in rice (2011)
  • Author(s): Ma, J. F.
  • Organizer: International Symposium of Rice functional Genomics 9th ISRFG in Taiwan
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Mechanism regulating HvAACT1 expression in barley (2011)
  • Author(s): Fujii, M., Yamaji, N., Ma, J. F.
  • Organizer: Rhizosphere 3 International Conference
  • Place of Presentation: Perth, Australia
• [Presentation] Isolation and characterization of Al-induced transporter gene in buckwheat (2011)
  • Author(s): Yokosho, K., Yamaji, N., Ma, J. F.
  • Organizer: Rhizosphere 3 International Conference
  • Place of Presentation: Perth, Australia
• [Presentation] Transporters involved in uptake and detoxification of minerals in plants (2011)
  • Author(s): Ma, J. F.
  • Organizer: Rhizosphere 3 International Conference
  • Place of Presentation: Perth, Australia
• [Presentation] イネ有害元素の集積に関与する遺伝子の同定と応用 (2011)
  • Author(s): 馬建鋒
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都
• [Presentation] ソバのアルミニウム誘導性トランスポーターの単離と機能解析 (2011)
  • Author(s): 横正健剛、山地直樹、馬建鋒
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: つくば
• [Presentation] Transporters involved in accumulation of toxic Cd and As in rice (2011)
  • Author(s): Ma, J. F.
  • Organizer: 2nd International Symposium on Genomics and Crop Genetic Improvement
  • Place of Presentation: Wuhan, China
• [Presentation] OsHMA3, a tonoplast-localized transporter for Cd, controls Cd transfer from the roots to the grains in rice (2011)
  • Author(s): Ma, J. F.
  • Organizer: 4th International IUPAC Symposium for Trace Elements in Food(TEF-4)
  • Place of Presentation: Aberdeen, Scotland
• [Presentation] OsHMA3, a tonoplast-localized transporter for Cd, controls Cd transfer from the roots to the grains in rice (2011)
  • Author(s): Ma, J.F.
  • Organizer: 4th International IUPAC Symposium for Trace Elements in Food (TEF-4)
  • Place of Presentation: Aberdeen, Scotland(招待講演)
• [Presentation] Transporters involved in accumulation of toxic Cd and As in rice (2011)
  • Author(s): Ma, J.F.
  • Organizer: 2nd International Symposium on Genomics and Crop Genetic Improvement
  • Place of Presentation: Wuhan, China(招待講演)
• [Presentation] Transporters involved in uptake and detoxification of minerals in plants (2011)
  • Author(s): Ma, J.F.
  • Organizer: Rhizosphere 3 International Conference
  • Place of Presentation: Perth, Australia(招待講演)
• [Presentation] Isolation and characterization of an Al-induced transporter gene in buckwheat (2011)
  • Author(s): Yokosho, K., Yamaji, N., Ma, J.F.
  • Organizer: Rhizosphere 3 International Conference
  • Place of Presentation: Perth, Australia
• [Presentation] Genes involved in the accumulation of toxic Cd and As in rice (2011)
  • Author(s): Ma, J.F.
  • Organizer: Inernational Symposium of Rice functional Genomics 9^<th> ISRPG in Taiwan
  • Place of Presentation: Taipei, Taiwan(招待講演)
• [Presentation] イネ有害元素の集積に関与する遺伝子の同定と応用 (2011)
  • Author(s): 馬建鋒
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] 植物の必須及び有害ミネラルのトランスポーター (2010)
  • Author(s): 馬建鋒
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] カドミウムトランスポーターの機能と構造 (2010)
  • Author(s): 馬建鋒
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Transporters involved in the accumulation and hypertolerance of Cd in plants, Plant Membrane Biology (2010)
  • Author(s): Ma, J. F., Ueno, D., Yamaji, N.
  • Organizer: 15th International Workshop Adelaide 2010
  • Place of Presentation: Adelaide, Australia
• [Presentation] Cloning and functional characterization of a tonoplast-localized Cd transporter in rice (2010)
  • Author(s): Ueno, D., Ma, J. F.
  • Organizer: 15th International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: Budapest, Hungary
• [Presentation] Cloning and functional characterization of a tonoplast-localized Cd transporter in rice (2010)
  • Author(s): Ueno, D., Ma, J.F.
  • Organizer: 15th International Symposium on Iron Nutrition and Interactions in Plants.
  • Place of Presentation: Budapest, Hungary
• [Presentation] Transporters involved in Al resistance in gramineous crops (2009)
  • Author(s): Ma, J. F., Huang, C. F., Furukawa, J., Yamaji, N.
  • Organizer: 7th International Symposium on Plant-Soil Interactions at Low pH.
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2009-05-20
• [Presentation] カドミウム超集積植物Thlaspi caerulescens(ecotype, Ganges)由来のP-type ATPase HMA3の単離と機能解析 (2009)
  • Author(s): 上野大勢・山地直樹・馬建鋒
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-23
• [Presentation] Cd超集積植物Thlaspi caerulescens(ecotype, Ganges)由来のP-type ATPase HMA3の機能 (2009)
  • Author(s): 上野大勢、山地直樹、馬建鋒
  • Organizer: 日本土壌肥料学会年会
  • Place of Presentation: 京都
• [Presentation] Transporters involved in Al resistance in gramineous crops (2009)
  • Author(s): Ma, J. F., Huang, C. F., Furukawa, J., Yamaji, N.
  • Organizer: 7th International Symposium on Plant-Soil Interactions at Low pH
  • Place of Presentation: Guangzhou, China
• [Book] 第4章不良土壌に対する植物の応答、1.酸性土壌,植物栄養学第2版 (2010)
  • Author(s): 馬建鋒, 間藤徹・藤原徹
  • Publisher: 文永堂
• [Remarks]
  • URL: http://www.rib.okayama-u.ac.jp/plant.stress/index-j.html