| Budget Amount *help |
¥46,410,000 (Direct Cost: ¥35,700,000、Indirect Cost: ¥10,710,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2009: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Research Abstract |
Isoquinoline alkaloid biosynthesis was investigated at molecular level to understand their biosynthetic pathways and its regulation. For this purpose, cDNAs of biosynthetic enzymes and transcription factors were isolated and characterized. Genes of biosynthetic enzymes isolated from C. japonicacells were successfully used to reconstitute novel alkaloid fermentation platform with some microbial enzyme genes to produce reticuline from glucose in microbial cells. In addition, bioassay system with Caenorhabditis elegans, to evaluate lipid-accumulation-reducing activity of isoquinoline alkaloids was successfully applied for the screening of new candidate among isoquinoline alkaloids.
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