| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2010: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2009: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Research Abstract |
Electrophiles in food have been implicated in human health and disease. They react with proteins and generate specific adduct structures that could function as ligands. Isothiocyanates, membrane-permeable electrophiles that form adducts with thiols, have been suggested to have important medical benefits. To gain a chemical insight into the cellular response induced by isothiocyanates, we designed a novel probe, combining an isothiocyanate-reactive group and an alkyne functionality, and revealed that the thiocarbamoylation of proteins occurred in the cells upon exposure to 6-HITC. The target of thiocarbamoylation included heat shock protein 90b (Hsp90b), a chaperone ATPase of the Hsp90 family implicated in protein maturation and targeting. Further study on the thiocarbamoylation of Hsp90b suggested that the formation of 6-HITC-Hsp90b conjugate might cause activation of heat shock factor-1, rapidly signaling a potential heat shock response.
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