Development and application of the shark single chain monoclonal antibody for the treatment of fish and shellfish diseases

• Project/Area Number: 21248025
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: AOKI Takashi 東京海洋大学, 海洋科学技術研究科, 特任教授 (00051805)
• Co-Investigator(Kenkyū-buntansha): 廣野 育生 東京海洋大学, 海洋科学技術研究科, 教授 (00270926) ; 近藤 秀裕 東京海洋大学, 海洋科学技術研究科, 准教授 (20314635)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥33,670,000 (Direct Cost: ¥25,900,000、Indirect Cost: ¥7,770,000); Fiscal Year 2011: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2009: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
• Keywords: 免疫学 / バイオテクノロジー / 感染症 / 水産学 / 免疫グロブリン / 新規抗原受容体(IgNAR) / サメ / 相補性決定領域3(CDR3) / ファージディスプレイライブラリー / 卵白リゾチーム(HEL) / 卵白リゾチーム

Research Abstract

Cartilaginous fish possess a unique immunoglobulin new antigen receptor(IgNAR) which is composed of only two heavy chains. Because of its simple and unique structure, the variable region of the shark IgNAR is suitable for the production of an artificial monoclonal antibody against fish and shellfish pathogens. Here, we cloned cDNA encoding IgNAR from the banded houndshark Triakis scyllium. The phage display library, possessing a variable region of the IgNAR, was constructed and clones specifically binding to a certain antigen were isolated

Research Products

• [Journal Article] Construction of an artificially randomized IgNAR phage display library : screening of variable regions that bind to hen egg white lysozyme (2012)
  • Author(s): Ohtani M, Hikima J, Jung TS, Kondo H, Hirono I, Aoki T
  • Journal Title: Mar Biotechnol(NY) Volume: 15 Issue: 1 Pages: 56-62
  • DOI: 10.1007/s10126-012-9456-1
• [Journal Article] cDNA cloning of the immunoglobulin heavy chain genes in banded houndshark Triakis scyllium (2010)
  • Author(s): Honda Y, Kondo H, Caipang CMA, Hirono I, Aoki T
  • Journal Title: Fish Shellfish Immunol Volume: 29 Pages: 854-861
• [Journal Article] cDNA cloning of the immunoglobulin heavy chain genes in bandedhoundshark Triakis scyllium. (2010)
  • Author(s): Honda Y, Kondo H, Caipang CM, Hirono I, Aoki T.
  • Journal Title: Fish Shellfish Immunol. Volume: 29 Pages: 854-861
  • Peer Reviewed
• [Journal Article] Identification of two distinct types of beta-2 microglobulin in marine fish, Pagrus major and Seriola quinqueradiata. (2010)
  • Author(s): Kondo H, Darawiroj D, TzehAGY, Yasuike M, Aoki T, HironoI.
  • Journal Title: Vet Immunol.Immunopathol. Volume: 134 Pages: 284-288
  • Peer Reviewed
• [Presentation] ランダム化したドチザメIgNARファージディスプレイライブラリーからの抗原特異的なクローンの選択 (2011)
  • Author(s): 大谷真紀、引間順一、近藤秀裕、廣野育生、Jung TS、青木宙
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学(長崎市)
  • Year and Date: 2011-10-01
• [Presentation] Isolation of antigen specific V-region molecule from artificially randomized IgNAR phage display library (2011)
  • Author(s): Ohtani M, Hikima J, Kondo H, Hirono I, Jung TS, Aoki T
  • Organizer: ヨーロッパ魚病学会大会
  • Place of Presentation: スプリット(クロアチア)
  • Year and Date: 2011-09-14
• [Presentation] Isolation of antigen specific V-region molecule from artificially randomized IgNAR phage display library (2011)
  • Author(s): Ohtani M, Hikima J, Kondo H, Hirono I, Jung TS, Aoki T
  • Organizer: 2011年ヨーロッパ魚病学会
  • Place of Presentation: スプリット(クロアチア)
  • Year and Date: 2011-09-14
• [Presentation] ドチザメIgNAR人工組換え体の作製に関する研究 (2011)
  • Author(s): 隅田慧光、廣野育生、青木宙、近藤秀裕
  • Organizer: 第14回マリンバイオテクノロジー学会大会
  • Place of Presentation: 於静岡県コンベンションアーツセンター(静岡市)
  • Year and Date: 2011-05-28
• [Presentation] ドチザメIgNAR人工組換え体の作製に関する研究 (2011)
  • Author(s): 隅田慧光、廣野育生、青木宙、近藤秀裕(海洋大)
  • Organizer: 第14回マリンバイオテクノロジー学会大会
  • Place of Presentation: 静岡県コンベンションアーツセンター(静岡市)
  • Year and Date: 2011-05-28
• [Presentation] ドチザメIgNAR可変領域の多様性に関する研究 (2009)
  • Author(s): 本多由佳、近藤秀裕、青木宙、廣野育生
  • Organizer: 日本魚病学会大会
  • Place of Presentation: 於東北大学(仙台市)
  • Year and Date: 2009-09-29
• [Presentation] ドチザメIgNAR可変領域の多様性に関する研究 (2009)
  • Author(s): 本多由佳・近藤秀裕・青木宙・廣野育生
  • Organizer: 日本魚病学会大会
  • Place of Presentation: 東北大学 (仙台)
  • Year and Date: 2009-09-28