Next generation of IVMFC technical development by identification of the microtubule control factor of oocyte.

• Project/Area Number: 21248032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tohoku University
• Principal Investigator: SATO Eimei 東北大学, 大学院・農学研究科, 教授 (80093243)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Masayuki 広島大学, 大学院・生物圏科学研究科, 准教授 (20314742) ; HOSHINO Yumi 東北大学, 大学院・農学研究科, 助教 (10451551)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥47,190,000 (Direct Cost: ¥36,300,000、Indirect Cost: ¥10,890,000); Fiscal Year 2011: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2010: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2009: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
• Keywords: 卵子 / 微小器官動態 / IVMFC 技術 / ギャップ結合 / ミトコンドリア / 卵成熟 / 受精能 / 発生能力 / Akt / 紡錘体 / 中心小体周辺物質 / dbcAMP / 体外成熟卵子 / IVMFC技術 / ギャップ結合制御因子 / ミトコンドリア配置制御因子

Research Abstract

Improve spectacularly of in vitro maturation (IVM) of an immature oocyte which makes the core of in vitro culture system of oocyte is aimed, it focused on localization, morphology and function of the microtubules (e.g. gap junction, nuclear membrane, chromosome, endoplasmic reticulum, spindle, mitochondria, cortical granule) of an immature oocyte, and identified the control agent. Furthermore, the chemical substance which controls expression of a control agent artificially was searched.

Research Products

• [Journal Article] Distribution of protein d isulfide isomerase during maturatio n of pig oocytes (2013)
  • Author(s): Ohashi,Y., Hoshino,Y., Tanemura, K, Sato,E
  • Journal Title: Anim Sci J. Volume: Vol.84, No.1 Issue: 1 Pages: 15-22
  • DOI: 10.1111/j.1740-0929.2012.01030.x
  • Peer Reviewed
• [Journal Article] Combination of co-culture with parthenogenetic embryo and aggregation enhances in vitro development and quality of miniature pig somatic cell nuclear transfer embryos (2012)
  • Author(s): Terashita, Y., Sugimura, S., Kudo, Y., Amano, R., Sato, E
  • Journal Title: Reprod.Biotechnol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Anew human spermatozoon selection method for intracytoplasmic sperm injection based on penetration into cervical mucus (2012)
  • Author(s): Takeda, N., Yoshii, N., Hoshino, Y., Tanemura, K., Sato, E., Odawara, Y
  • Journal Title: J.Mamm.Ova Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Leukocyte count in follicular aspirate samples could be a prognostic biomerker of the developmental competence of human metaphase II oocytes (2012)
  • Author(s): Ishikawa, T., Doshida, M., Kyoya, T., Takizawa, T., Sakura, M., Hoshino, Y., Tanemura, K., Kyono, K., Sato, E
  • Journal Title: J.Reprod.Engineer Volume: (in press)
  • Peer Reviewed
• [Journal Article] Improving the quality of miniature pigsomatic cell nuclear transfer blastocysts: aggregation of SCNT embryos at the four-cell stage (2011)
  • Author(s): Terashita,Y., Sugimura,S., Kudo,Y., Amano,R., Hiradate, Y., Sato, E.
  • Journal Title: Teprod.Domest.Anim Volume: Vo.46, No.2 Issue: 2 Pages: 189-196
  • DOI: 10.1111/j.1439-0531.2010.01614.x
  • Peer Reviewed
• [Journal Article] Anomalous oxygen consumption in porcine somatic cell, Cell (2010)
  • Author(s): Sugimura, S., Yokoo,M., Yamanaka,K., Kawahara,M., Moriyasu,S., Wakai,T., Nagai,T., Abe,H., Sato,E
  • Journal Title: Reprogram Volume: Vol. 12, No.4 Issue: 4 Pages: 463-474
  • DOI: 10.1089/cell.2009.0111
  • Peer Reviewed
• [Journal Article] Identification and expression analysis of CX45 and CX60 as major connexins in porcine oocytes (2010)
  • Author(s): Nitta,M., Yogo,K., Ohashi,M., Akiyama,M., Kunitomo,Y., Ogawa,T., Ishida-Kitagawa,N., Miyoshi,J., Sato,E., Takeya,T
  • Journal Title: J.Anim.Sci Volume: Vol.88, No. 10 Issue: 10 Pages: 3269-3279
  • DOI: 10.2527/jas.2009-2781
  • Peer Reviewed
• [Journal Article] 家畜繁殖学の挑戦と近未来 (2010)
  • Author(s): 佐藤英明
  • Journal Title: ブレインテクノニュース Volume: No.14 Pages: 1-6
  • URL: http://jglobal.jst.go.jp/public/20090422/201002227784400104
• [Journal Article] Akt expression in mouse oocytes matured in vivo and in Vitro (2010)
  • Author(s): Cecconi,S,, Rossi,G., Santilli,A., Stefano,LD., Hoshino,Y., Sato,E., Palmerini MG, Macchiarelli G
  • Journal Title: RBM Online Volume: Vol.20, No.1 Issue: 1 Pages: 54-41
  • DOI: 10.1016/j.rbmo.2009.10.011
  • Peer Reviewed
• [Journal Article] Akt expression in mouse oocytes matured in vivo and in vitro. (2010)
  • Author(s): 佐藤英明
  • Journal Title: RBM Online (in press)
  • Peer Reviewed
• [Journal Article] Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos. (2010)
  • Author(s): 佐藤英明
  • Journal Title: Cell.Reprogram. (in press)
  • Peer Reviewed
• [Journal Article] Improving the quality of miniature pig somatic cell nuclear transfer blastocysts : aggregation of SCNT embryos at the four-cell stage. (2010)
  • Author(s): 佐藤英明
  • Journal Title: Teprod.Domest.Anim. (in press)
  • Peer Reviewed
• [Journal Article] Combination of co-culture with parthenogenetic embryo and agregation enhances in vitro development and quality of miniature pig somatic cell nuclear transfer embryos
  • Author(s): Terashita,Y., Sugimura,S., Kudo,Y., Amano,R., Sato,E
  • Journal Title: Reprod.Biotechnol Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Odawara,Y, Ane w human spermatozoon selection meth od for intracytoplasmic sperm injec tion based on penetration into cervical mucus
  • Author(s): Takeda,N., Yoshii,N., Hoshino,Y., T anemura,K., Sato,E.
  • Journal Title: J.Mamm.Ova Res. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Leukocyte count in follicular aspirate samples could be a prognostic biom erker of the developmental competence of human metaphase II oocytes
  • Author(s): Ishikawa,T., Doshida,M., Kyoya,T., Takizawa,T., Sakura,M., Hoshino,Y., Tanemura,K., Kyono,K., Sato,E
  • Journal Title: J. Reprod.Engineer. Volume: (印刷中)
  • Peer Reviewed
• [Presentation] P31 inhibition of mTORinduces cumulus expansion and meiotic maturation in mouse oocytes withoutgonadotropin stimulation. AnnualDconference 2010 of Society forReproductionand Fertility (2011)
  • Author(s): Sato, E
  • Organizer: Annual Dconference 2010 of Society for Reproduction and Fertility
  • Place of Presentation: University of Nottingham, UK.
  • Year and Date: 2011-07-11
• [Presentation] Role of capillary blood vessels and macrophages in follicular development and atresia (2011)
  • Author(s): Sato, E
  • Organizer: 16th ICBAR
  • Place of Presentation: Sri Lanka, Kandy.
  • Year and Date: 2011-04-05
• [Presentation] Role of capillary blood vessels and macrophages in follicular development and atresia.16th ICBAR (2011)
  • Author(s): Sato, E.
  • Organizer: 16th ICBAR
  • Place of Presentation: Sri Lanka、Kandy
• [Presentation] Molecular basis of follic ular growth and oocyte maturation-Use of farm animals for biology andbiomedical sience (2010)
  • Author(s): Sato, E
  • Organizer: 3rd Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2010)
  • Place of Presentation: Pattaya, Thailand.
  • Year and Date: 2010-04-09
• [Presentation] Molecular basis of follicular growth and oocyte maturation -Use of farm animals for biology and biomedical sience. 3rd Congress of the Asia Pacific Initiative on Reproduction(ASPIRE 2010) (2010)
  • Author(s): 佐藤英明
  • Organizer: Asia Pacific Initiative on Reproduction
  • Place of Presentation: Pattaya, Thailand
• [Book] 排卵と受精、生殖卵巣学(石塚文平、鈴木秋悦編) (2011)
  • Author(s): 佐藤英明
  • Publisher: 医歯薬出版
• [Book] 排卵と受精、生殖卵巣学(石塚文平:鈴木秋悦編) (2011)
  • Author(s): 佐藤英明
  • Publisher: 医歯薬出版
• [Book] カラーアトラス不妊治療のための卵子学 (2010)
  • Author(s): 星野由美、佐藤英明(鈴木秋悦編)
  • Publisher: 医歯薬学出版株式会社
• [Book] カラーアトラス不妊治療のための卵子学 (2010)
  • Author(s): 佐藤英明
  • Total Pages: 208
  • Publisher: 医歯薬出版
• [Remarks]
  • URL: http://db.tohoku.ac.jp/whois/detail/34b0b77a410b78164106dee5e216fef4.html
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/j030100/id0027.html