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Molecular biology of microbial degradation of 2,3,7,8-TCDD for effective remediation of polychlorinated dioxins-contaminated areas.

Research Project

Project/Area Number 21248037
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionNihon University (2010-2012)
Tokyo University of Agriculture and Technology (2009)

Principal Investigator

KATAYAMA Yoshihiro  日本大学, 生物資源科学部, 教授 (10214339)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masaya  (独)森林総合研究所, きのこ微生物研究領域, 微生物機能解析チーム長 (50353793)
OHTSUKA Yuichiro  (独)森林総合研究所, バイオマス化学研究領域, 主任研究員 (80455261)
TAKAHASHI Atsushi  高砂熱学工業株式会社, 常務執行役員 (60501369)
SATO Kanna  東京農工大学, 農学研究院, 助教 (40456603)
KAJITA Shinya  東京農工大学, 農学研究院, 准教授 (40323753)
保科 定頼  東京慈恵会医科大学, 医学部, 准教授 (30119846)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Keywordsダイオキシン / 環境浄化 / 2,3,7,8-tetrachlorodibenzo-p-dioxin / 2,7,-dichlorodibenzo-p-dioxin / ダイオキシン蛍光アナログ / ダイオキシン還元解裂酵素 / 遺伝子ライブラリー / 遺伝子クローニング / ,3,7,8-TCDD / 2,7-DCDD / phytoremediation / 蛍光アナログ / 酵素遺伝子 / 植物 / ダイオキシ / 2,7-dichlorodibenzo-p-dioxin / 2,3,7.8-tetrachlorodibenzo-p-dioxin
Research Abstract

In this study, we are the first to demonstrate that Geobacillus sp. UZO3 cell free extract reductively cleavages the diaryl ether bonds of 2,3,7,8-TCDD in a sequential fashion. Furthermore, we succeeded in characterization of the gene, which contains an open reading frame of 351 bp encoding 116 amino acids. The enzyme has a CoA-binding site and reductively cleavages2,3,7,8-TCDD using CoA as the hydrogen donor.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (17 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Develpoment of Highly Sensitive Assay for Enzyme-Mediated Reductive Degradationof Polychlorinated Dibenzo-p-dioxin2012

    • Author(s)
      Y.Suzuki, M.Nakamura, Y.Otsuka, N.Suzuki, K.Ohyama, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, T.Fujii, A.Takahashi, Y.Katayama
    • Journal Title

      Environmental Toxicology and Chemistry.

      Volume: 31 Issue: 5 Pages: 1072-1075

    • DOI

      10.1002/etc.1775

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Develpoment of Highly Sensitive Assay for Enzyme-Mediated Reductive Degradation of Polychlorinated Dibenzo-p-dioxin2012

    • Author(s)
      Y.Suzuki
    • Journal Title

      Environmental Toxicology and Chemistry.

      Volume: 31 Pages: 1072-1075

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial Degradation of 2,7-dichlorodibenzo-p-dioxin by Reductive Cleavage of Diaryl Ether Bonds2012

    • Author(s)
      Y.Otsuka,
    • Journal Title

      Organohalogen Compounds

      Volume: 74 Pages: 600-604

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent Assay for New Dioxin Degrading Microorganism2012

    • Author(s)
      Y.Suzuki
    • Journal Title

      Organohalogen Compounds

      Volume: 74 Pages: 1221-1224

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic activity of cell free extract from Geobacillus sp.UZO 3 catalyzes reductive cleavage of diaryl ether bonds2,3,7,8-tetrachlorodibenzo- p-dioxin2011

    • Author(s)
      Y.Suzuki, M.Nakamura, Y.Otsuka, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, Y.Okamura, A.Takahashi, Y.Katayama
    • Journal Title

      Organohalogen Compounds.

      Volume: 73 Pages: 1400-1403

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel enzymatic activity of cell free extract from thermophilic Geobacillus sp. UZO 3 catalyzes reductive cleavage of diaryl etherbonds of 2,7-dichlorodibenzo-p-dioxin2011

    • Author(s)
      Y.Suzuki, M.Nakamura, Y.Otsuka, N.Suzuki,K.Ohyama,T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, T.Fujii, A.Takahashi, Y.Katayama
    • Journal Title

      Chemosphere.

      Volume: 83 Pages: 868-872

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic activity of cell free extract from Geobacillus sp.UZO 3 catalyzes reductive cleavage of diaryl etherbonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin2011

    • Author(s)
      Y.Suzuki, M.Nakamura, Y.Otsuka, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, Y.Okamura, A.Takahashi, Y.Katayama
    • Journal Title

      Organohalogen Compounds

      Volume: 73 Pages: 1400-1403

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel enzymatic activity of cell free extract from thermophilic Geobacillus sp.UZO 3 catalyzes reductive cleavage of diaryl ethebonds of 2,7-dichlorodibenzo-p-dioxin2011

    • Author(s)
      Y.Suzuki, M.Nakamura, Y.Otsuka, N.Suzuki, K.Ohyama, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, T.Fujii, A.Takahashi, Y.katayama
    • Journal Title

      Chemosphere

      Volume: 83 Pages: 868-872

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pre-treatment Method in a Bio-degradation System for Extracting Polychlorinated Dioxins from Contaminated Soils and Sediments2010

    • Author(s)
      A.Takahashi、T.Kameyama, S.Hoshina, Y.Katayama
    • Journal Title

      Proceedings Venice 2010, Third International Symposium on Energy from Biomass and Waste

      Volume: PE13/CD Pages: 1-12

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Pre-treatment Method in a Bio-degradation System for Removing Poly-chlorinated Dioxins from Contaminated Soil and Sediments2009

    • Author(s)
      A.Takahashi, T.Kameyama, S.Hoshina, Y.Katayama
    • Journal Title

      Organohalogen Compounds Vol.71

      Pages: 109-112

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Functional analysis of dioxin-degrading microorganism using the fluorometoric substrate and chlorinated dioxin.2009

    • Author(s)
      Yuichiro Otsuka, Masaya Nakamura, 他8名
    • Journal Title

      Journal of Bioscience and Bioengineering 108

      Pages: 89-89

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorometric assay substrate for dioxin degrading microorganisms-synthesis of dioxin-analogous fluorometric assay substrate.2009

    • Author(s)
      Masaya Nakamura, Yuichiro Otsuka, 他8名
    • Journal Title

      Journal of Bioscience and Bioengineering 108

      Pages: 91-91

    • NAID

      210000003500

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Geobacillus sp. UZO 3 株が持つダイオキシン・2,3,7,8-TCDD 分子内ジアリールエーテル結合還元開裂酵素の生化学的・遺伝学的研究2013

    • Author(s)
      鈴木悠造
    • Organizer
      日本農芸化学会2013年度大会
    • Related Report
      2012 Annual Research Report
  • [Presentation] Microbial Degradation of 2,7-dichlorodi benzo-p-dioxin by Reductive Cleavage of Diaryl Ether Bonds2012

    • Author(s)
      Y.Otsuka, Y.Suzuki, M.Nakamura, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, A.Takahashi, Y.Katayama
    • Organizer
      32nd International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2012)
    • Place of Presentation
      Cairns,Australia
    • Related Report
      2012 Final Research Report
  • [Presentation] Microbial Degradation of 2,7-dichlorodibenzo-p-dioxin by Reductive Cleavage of Diaryl Ether Bonds2012

    • Author(s)
      Y.Otsuka,
    • Organizer
      32nd International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2012)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Fluorescent Assay for New Dioxin Degrading Microorganism2012

    • Author(s)
      Y.Suzuki,
    • Organizer
      32nd International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2012)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pre-treatment method in a bio-degradation system for extracting polychlorinated dioxins from contaminated soil and sediments2012

    • Author(s)
      A.Takahashi
    • Organizer
      Fourth International Symposium On Energy From Biomass And Waste (Venice 2012)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bio-degradation System for Poly-chlorinated Dibenzo-p-dioxins and Dibenzofurans by Using Crude Enzyme Extracted from Geobacillus sp.UZO 32011

    • Author(s)
      A.Takahashi, T.Kameyama, Y.Suzuki, M.Nakamura, Y.Otsuka, Y.katayama
    • Organizer
      Sardinia 2011 Symposium, IWWG (International Waste Working Group)
    • Place of Presentation
      Sardinia, Italy
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enzymatic Activity of Cell Free Extract from Geobacillus sp.UZO3 Catalyses Reductive Cleavage of Diaryl Ether Bonds of 2,3,7,8-tetrachlorodibenzo-p-dioxin2011

    • Author(s)
      Y, Suzuki, M.Nakamura, Y.Otsuka, t.Kawakami, K.Sato, S.Kajita, S.Hishiyama, Y.Okamura, A.Takahashi, Y.Katayama
    • Organizer
      31^<st> International Symposium on Haloganated Persistent Organic Pollutants
    • Place of Presentation
      Brussels, Belgium
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reductive Cleavage of Diaryl Ether Bonds of 2,7-Dichlorodibenzo-p-dioxin by Cell Free Extracts from Geobacillus sp.UZO32011

    • Author(s)
      M.Nakamura, Y.Suzuki, Y.Otsuka, K.Sato, S.Kajita, A.Takahashi, Y.Katayama
    • Organizer
      nternational Union of Microbiological Societies 2011 Congerss
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Annual Research Report
  • [Presentation] Novel Enzymatic Activity of Cell-Free Extract from Thermophilic Geobacillus sp UZO 3 Catalyzes Reductive Cleavage of Diaryl Ether Bonds of2,7-Dichlorodi benzo-p-Dioxin2010

    • Author(s)
      M.Nakamura, Y.suzuki, Y.Otsuka, T.Kawakami, K. Sato, S.Kajita, S.Hishiyama. A.Takahashi, Y.Katayama
    • Organizer
      International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Honollulu,USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Enzymatic Activity of Cell Free Extract from Thermophilic Geobacillus sp.SH2B-J2 Catalyzes Reductive Cleavage of Diaryl Ether Bonds of 2,7-Dichlorodibenzo-p-Dioxin2010

    • Author(s)
      Y.Suzuki, M.Nakamura Y.Otsuka, T.Sonoki, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, S.Hoshina, A.Takahashi, Y.Katayama
    • Organizer
      14th International Biothechnology Symposium and Exhibition IBS2010
    • Place of Presentation
      Rimini Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pre-treatment Method in a Bio-degradation System for Extracting Polychlorinated Dioxins from Contaminated Soils and Sediments2010

    • Author(s)
      A.Takahashi、T.Kameyama, S.Hoshina, Y.Katayama
    • Organizer
      Third International Symposium on Energy Biomass and Waste
    • Place of Presentation
      Venice Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel Enzymatic Activity of Cell-Free Extract from Thermophilic Geobacillus sp UZO 3 Catalyzes Reductive Cleavage of Diaryl Ether Bonds of 2,7-Dichlorodibenzo-p-Dioxin2010

    • Author(s)
      M.Nakamura, Y.suzuki, Y.Otsuka, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, A.Takahashi, Y.Katayama
    • Organizer
      International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Honollulu (Hawaii) USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 塩素化ダイオキシン分解微生物Geobacillus sp.SH2B-J2株のエーテル結合開裂能の解析2010

    • Author(s)
      鈴木悠造、梅香佑輔、佐藤かんな、梶田真也、大塚祐一郎、菱山正二郎、中村雅哉、保科定頼,川上健志、高橋惇、片山義博
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      シーガイア、宮崎
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional analysis of dioxin-degrading microorganism using the fluorometoric substrate and chlorinated dioxin.2009

    • Author(s)
      Yuichiro Otsuka, Masaya Nakamura, 他8名
    • Organizer
      アジア太平洋生物工学会議(APBioChEC '09)
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-11-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Fluorometric assay substrate for dioxin degrading microorganisms-synthesis of dioxin-analogous fluorometric assay substrate.2009

    • Author(s)
      Masaya Nakamura, Yuichiro Otsuka, 他8名
    • Organizer
      アジア太平洋生物工学会議(APBioChEC '09)
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-11-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Pre-treatment Method in a Bio-degradation System for Removing Poly-chlorinated Dioxins from Contaminated Soil and Sediments2009

    • Author(s)
      A.Takahashi, T.Kameyama, S.Hoshina
    • Organizer
      12^<th> International Waste Management and Landfill Symposium(Sardinia2009)
    • Place of Presentation
      Cagliari(Italy)
    • Year and Date
      2009-09-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] Pre-treatment Method in a Bio-degradation System for Removing Poly-chlorinated Dioxins from Contaminated Soil and Sediments2009

    • Author(s)
      A.Takahashi, T.Kameyama, S.Hoshina, Y.Katayama
    • Organizer
      29^<th> International Symposium on Halogenated Persistent Organic Pollutants(Dioxin2009)
    • Place of Presentation
      北京(中国)
    • Year and Date
      2009-08-24
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ダイオキシン類分解剤およびダイオキ2012

    • Inventor(s)
      片山義博,中村雅哉,大塚裕一郎,鈴木悠造,高橋惇
    • Industrial Property Rights Holder
      日本大学、(独)森林総合研究所、高砂熱学工業株式会社、環テック株式会社
    • Industrial Property Number
      2012-053305
    • Acquisition Date
      2012-03-09
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] ダイオキシン類分解剤およびダイオキシン類の分解方法2012

    • Inventor(s)
      片山義博, 中村雅哉, 大塚裕一郎, 鈴木悠造, 高橋惇
    • Industrial Property Rights Holder
      日本大学(独)森林総合研究所、(株)高砂熱学工業、環テック株式会社
    • Industrial Property Number
      2012-053305
    • Filing Date
      2012-03-09
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2022-11-04  

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