Basic Studies on Mammalian Fertilization and Implantation

• Project/Area Number: 21248038
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: BABA Tadashi 筑波大学, 生命環境系, 教授 (40165056)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000); Fiscal Year 2012: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2010: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2009: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: マウス / 受精 / 着床 / 精子 / 卵子

Research Abstract

This study has focused on the molecular mechanisms on mammalian fertilization and implantation. On the basis of the results obtained, sperm entry into the cumulus mass may be dependent on capacitation status of sperm. The present study also proposes that fertilizing sperm complete acrosomal exocytosis in the cumulus matrix and on the oocyte zona pellucida before fusing with the oocyte. A factor that facilitates sperm/oocyte fusion has been identified in uterine and oviductal fluids. Moreover, activation of ERalpha in the ERK1/2 pathway may play an important role in embryo implantation.

Research Products

• [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse (2013)
  • Author(s): Kanemori, Y., Ryu, J.-H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 88 Issue: 4 Pages: 1-8
  • DOI: 10.1095/biolreprod.112.107425
  • Peer Reviewed
• [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse (2013)
  • Author(s): Yoshinori Kanemori
  • Journal Title: Biology of Reproduction Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization. (2012)
  • Author(s): Zhou, C., Kang, W., and Baba, T
  • Journal Title: J. Reprod. Dev. Volume: 58 Pages: 330-337
  • NAID: 10030821330
  • URL: https://www.jstage.jst.go.jp/article/jrd/58/3/58_2011-006/_article
  • Peer Reviewed
• [Journal Article] Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis (2012)
  • Author(s): Hasegawa, H., Noguchi, J., Yamashita, M., Okada, R., Sugimoto, R., Furuya, M., Unoki, T., Funakoshi, Y., Baba, T., and Kanaho, Y.
  • Journal Title: Biol. Reprod Volume: 86 Issue: 5 Pages: 1-12
  • DOI: 10.1095/biolreprod.110.089896
  • Peer Reviewed
• [Journal Article] Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization (2012)
  • Author(s): Chon Zhou
  • Journal Title: Journal of Reproduction and Development Volume: 58 Pages: 330-337
  • Peer Reviewed
• [Journal Article] Expression analysis of MIF4GD in the rat testis (2011)
  • Author(s): Okada, K., Kimura, M., Moriyama, Y., Nakai, M., Kikuchi, K., Kaneko, H., Kunieda, T., Baba, T., and Noguchi, J
  • Journal Title: J. Reprod. Dev. Volume: 57 Pages: 256-261
  • NAID: 10028275441
  • URL: https://www.jstage.jst.go.jp/article/jrd/57/2/57_10-138H/_article
  • Peer Reviewed
• [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro (2010)
  • Author(s): Kawano, N., Kang, Wj., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, K., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 83 Issue: 3 Pages: 359-369
  • DOI: 10.1095/biolreprod.109.083089
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme. (2010)
  • Author(s): Akama K, Horikoshi T, Sugiyama A, Nakahata S, Akitsu A, Niwa N, Intoh A, Kakui Y, Sugaya M, Takei K, Imaizumi N, Sato T, Matsumoto R, Iwahashi H, Kashiwabara SI, Baba T, Nakamura M, Toda T.
  • Journal Title: Biochim Biophys Acta. Volume: 1804(6) Issue: 6 Pages: 1272-1284
  • DOI: 10.1016/j.bbapap.2010.02.004
  • Peer Reviewed
• [Journal Article] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? (2010)
  • Author(s): Kang, W., Zhou, C., Koga, Y., and Baba, T.
  • Journal Title: J. Reprod. Dev Volume: 56 Pages: 140-144
  • NAID: 10026323923
  • URL: https://www.jstage.jst.go.jp/article/jrd/56/1/56_09-152N/_article
  • Peer Reviewed
• [Journal Article] Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro (2010)
  • Author(s): Kawano, N., Kang, W., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado, M., Baba, T.
  • Journal Title: Biology of Reproduction Volume: 83 Pages: 359-369
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme (2010)
  • Author(s): Akama, K., Horikoshi, T, Sugiyama, A., Nakahata, S., Akitsu, A., Niwa, N., Intoh, A., Kakui, Y., Sugaya, M., Takei, K., Imaizumi, N., Sato, T, Matsumoto, R., Iwahashi, H., Kashiwabara, S., Baba, T., Nakamura, M., Toda, T.
  • Journal Title: Biochimica et Biphysica Acta Volume: 1804 Pages: 1272-1284
  • Peer Reviewed
• [Journal Article] Functional roles of sperm hyaluronidase, HYAL5 and SPAM1, in mouse fertilization (2009)
  • Author(s): Kimura, M., Kim, E., Kang,WJ., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., and Baba, T.
  • Journal Title: Biol. Reprod Volume: 81 Issue: 5 Pages: 939-947
  • DOI: 10.1095/biolreprod.109.078816
  • Peer Reviewed
• [Journal Article] Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization. (2009)
  • Author(s): Kimura, M., Kim, E., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., Baba, T.
  • Journal Title: Biology of Reproduction Volume: 81 Pages: 939-947
  • Peer Reviewed
• [Journal Article] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? (2009)
  • Author(s): Kang, W., Zhou, C., Koga, Y., Baba, T.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 140-144
  • NAID: 10026323923
  • Peer Reviewed
• [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization (2012)
  • Author(s): Ryu, J. -H., Sudo, M., Kanemori, Y., Kashiwabara, S., Baba, T.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-14
• [Presentation] Sperm penetration through the cumulus matrix (2012)
  • Author(s): Baba, T
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
  • Place of Presentation: 名古屋ガーデンパレス
  • Year and Date: 2012-11-13
• [Presentation] TZF遺伝子欠損マウスは雄性不妊である (2011)
  • Author(s): 吉田薫、佐古典久、石塚晶道、大塚絵里、井上敦人、小高みれい、大島裕隆、田村徳久、馬場忠、柏原真一、岡部勝、野口純子、萩原啓実
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] 精子の卵丘細胞層通過でのヒアルロニダーゼとセリンプロテアーゼによる協働的な作用 (2011)
  • Author(s): 周崇、康宇鎭、馬場忠
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Localization of a PSMA8-containing proteasome in mouse sperm (2011)
  • Author(s): Koyama, M., Koga, Y., Hyodo, H., Yamashita, M., Ogichi, K., Kanemori, Y., Sawada H., Baba, T
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Proacrosin-binding protein sp32/ACRBP is involved in acrosome biogenesis of mouse sperm (2011)
  • Author(s): Ryu, J. H, Sudou, M., Koga, Y., Kanemori, Y., Baba, T
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Characterization of a novel PSMA8-containing proteasome present in mouse sperm (2011)
  • Author(s): Koga, Y., Koyama, M., Hyodo, H., Yamashita, M., Sawada, H., Baba, T
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: ケアンズコンベンションセンター
  • Year and Date: 2011-10-10
• [Presentation] マウスの2回目以降の着床箇所は着床痕によって制御される (2010)
  • Author(s): 山下美鈴、馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 精巣特異的サブユニットPSMA8を含むプロテアソームの局在と機能の解析 (2010)
  • Author(s): 小山真奈、古賀義隆、兵藤仁美、横田直人、澤田均、馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 卵子透明帯顆粒が卵丘細胞層マトリクスに存在する (2010)
  • Author(s): 古賀義隆,河野菜摘子,康宇鎭,津村啓子,山下美鈴,馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 受精とプロテオリシス (2010)
  • Author(s): 馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] マウス精子先体反応でのダイズトリプシンインヒビターの標的タンパク質 (2010)
  • Author(s): 和泉裕一、山下美鈴、康宇鎭、馬場忠
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] The trypsin-like serine protease activity of ACR and PRSS21 is important but not essential for fertilization in vivo (2010)
  • Author(s): Kang, W., Kawano, N., Yamashita, M., Koga, Y., Yamazaki, T., Hata, T., Miyado,K., Baba, T
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] 受精とプロテオリシス (2010)
  • Author(s): 馬場忠
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)(招待講演)
  • Year and Date: 2010-12-07
• [Presentation] Effects of placental scars on implantation in multiparous mice (2010)
  • Author(s): Yamashita, M., Baba, T
  • Organizer: Gordon Research Conference on Reproductive Tracts Biology
  • Place of Presentation: Proctor Academy, New Hampshire, USA
  • Year and Date: 2010-08-16
• [Presentation] Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization? (2009)
  • Author(s): Kang, W., Zhou, C., Koga, Y., Baba, T
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Role of sperm serine protease in fertilization (2009)
  • Author(s): Koga, Y., Kawano, N., Yamashita, M., Kang, W., Baba, T
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Recognition system of mammalian sperm in the female reproductive tract (2009)
  • Author(s): Baba, T
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] 子宮内因子による受精能回復機構の解析 (2009)
  • Author(s): 山下美鈴、馬場忠
  • Organizer: 第82回日本生化学学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] Functional roles of sperm hyaluronidase, HYAL5 and SPAM1 (2009)
  • Author(s): Kang, W., Kimura, M., Kim, E., Yamashita,M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., Baba, T
  • Organizer: in mouse fertilizationSociety for the Study of Reproduction 42ndAnnual Meeting
  • Place of Presentation: ピッツバーグコンベンションセンター、ペンシルバニア
  • Year and Date: 2009-07-18
• [Presentation] Functional effects of uterine fluids on PRSS21-deficient sperm (2009)
  • Author(s): Yamashita, M., Kang, W., Kashiwabara, S.,Baba, T
  • Organizer: Gordon Research Conference on Fertilization and Activation of Development
  • Place of Presentation: ホルダーネススクール、ニューハンプシャー
  • Year and Date: 2009-07-14
• [Presentation] Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in Mouse fertilization. (2009)
  • Author(s): Tadashi Baba
  • Organizer: 20th International Symposium on Glycobiology
  • Place of Presentation: Puerto Rico
• [Presentation] Sperm penetration through the cumulus matrix
  • Author(s): Tadashi Baba
  • Organizer: International Symposium on the Mechanisms of Sexual Reproduction in Animals and and Plants
  • Place of Presentation: 名古屋ガーデンパレス
  • Invited
• [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization
  • Author(s): Jin Hyeob Ryu
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 精子の卵丘細胞層通過でのヒアルロニダーゼとセリンプロテアーゼによる協働的な作用
  • Author(s): 周 崇
  • Organizer: 第３４回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
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