Studies of pathological involvement of Sox9 in osteoarthritis and discovery of new treatment for osteoarthritis

• Project/Area Number: 21249078
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: AKIYAMA Haruhiko 京都大学, 医学研究科, 准教授 (60402830)
• Co-Investigator(Kenkyū-buntansha): YASODA Akihiro 京都大学, 医学研究科, 講師 (50378642) ; ITO Hiromu 京都大学, 医学研究科, 准教授 (70397537)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000); Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2011: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
• Keywords: 変形性関節症 / Sox9 / 遺伝子改変マウス

Research Abstract

Overexpression of Sox9 in articular chondrocytes resulted in delay of osteoarthritis change. In addition, genome-wide screening using GeneChip with chondrocytes derived mouse articular cartialge, Sox9-EGFP mice, and Sox9-EGFP null chimera identified one gene involved in osteoarthritis and one gene induced by Sox9. Furthermore, screening of about 10000 chemical reagents using Sox9-EGFP chondrocytes revealed 7 chemical reagents which has Sox9-indecing activity.

Research Products

• [Journal Article] Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25. (2011)
  • Author(s): Yukio Nakamura, Koji Yamamoto, Xinjun He, Bungo Otsuki, Youngwoo Kim, Hiroki Murao, Tsunemitsu Soeda, Noriyuki Tsumaki, Jian Min Deng, Zhaoping Zhang, Richard R. Behringer, Benoit de Crombrugghe, John H. Postlethwait, Matthew L. Warman, Takashi Nakamura
  • Journal Title: Nature Communications 2 Volume: Article number: 251 Issue: 1 Pages: 251-251
  • DOI: 10.1038/ncomms1242
  • Peer Reviewed
• [Journal Article] Continuous cell supply from a Sox9-expressing progenitor zone in adult liver, exocrine pancreas and intestine (2011)
  • Author(s): Kenichiro Furuyama, et al
  • Journal Title: Nature Genetics Volume: 43 Issue: 1 Pages: 34-41
  • DOI: 10.1038/ng.722
  • Peer Reviewed
• [Journal Article] Generation of transgenic mice for conditional overexpression of Sox9 (2011)
  • Author(s): Youngwoo Kim, Hiroki Murao, Haruhiko Akiyama., et al.
  • Journal Title: J Bone Miner Metab Volume: 29(1) Pages: 123-129
  • NAID: 10029023999
  • Peer Reviewed
• [Journal Article] Sox9-expressing Prectusors are the Cellular Origin of the Cruciate Ligament of the Knee Joint and the Limb Tendons (2010)
  • Author(s): Tsunemitsu Soeda, Jian Min Deng, Haruhiko Akiyama., et al.
  • Journal Title: Genesis Volume: 48(11) Pages: 635-644
  • Peer Reviewed
• [Presentation] TRPV4 はSox9 の発現を介して変形性関節症の発症を抑制する. (2012)
  • Author(s): 山本 浩司, 金 永優, 武井 大輔, 村尾浩樹, 末吉 達也, 塚中 真佐子, 水野 敦子, 鈴木 誠, 松田 秀一, 秋山 治彦
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-10-27
• [Presentation] 軟骨における転写制御機構から病態解析・治療へ (2010)
  • Author(s): 秋山治彦
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-22