Possible involvement of a new molecule, PRIP in exocytosis
Project/Area Number |
21249089
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional basic dentistry
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Research Institution | Kyushu University |
Principal Investigator |
HIRATA Masato 九州大学, 歯学研究院, 教授 (60136471)
|
Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Hiroshi 九州大学, 大学院・歯学研究院, 准教授 (70304813)
MATSUDA Miho 九州大学, 大学院・歯学研究院, 助教 (40291520)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2011: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2009: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
|
Keywords | 開口分泌 / SNARE / 膜リン脂質 / PHドメイン / C2ドメイン / リン酸化 / カルシウム / イノシトールリン脂質 / SNAP-25 / イノシトールリン脂 |
Research Abstract |
We have isolated a novel protein, PRIP(PLC-related but catalytically inactive protein), followed by generation of the gene-deficient mice(KO mice). KO mice exhibited up-regulation of gonadotropins and insulin, indicating that PRIP might be involved in events common to vesicular fusion processes. In the current studies, therefore, we have explored the mechanisms by which PRIP is involved in exocytosis. The results suggest that PRIP is negatively involved in the dense-core vesicle secretion of PC12 cells, by occupying the membrane phospholipids required for the secretion with the PH domain, and interfering SNARE proteins by the C2 domains. Furthermore, PRIP is involved in the phospho-modulation of exocytosis by regulating activities of protein phosphatases.
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Report
(4 results)
Research Products
(60 results)
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[Journal Article] Second basic pockets contribute to the localization of PX domains by binding to phosphatidic acid2012
Author(s)
Takeuchi, H., Zhang, Z., Gao, J., Sugiyama, G., Takeuchi, T., and Hirata, M.
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Journal Title
Adv. Enz. Regul
Volume: 52
Pages: 183-194
NAID
Related Report
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[Journal Article] DIF-1 inhibits the Wnt/υ-catenin signaling pathway by inhibiting TCF7L2 expression in colon cancer cell lines2012
Author(s)
Jingushi, K., Takahashi-Yanaga, F., Yoshihara, T., Shiraishi, F., Watanabe, Y., Hirata, M., Morimoto, S., and Sasaguri, T.
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Journal Title
Biochem. Pharmacol
Volume: 83
Pages: 47-56
Related Report
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[Journal Article] Dysfunction of extrasynaptic GABAergic transmission in PRIP-1 KO mice is associated with an epilepsy phenotype2012
Author(s)
Zhu, G., Yoshida, S., Migita, K., Yamada, J., Mori, F., Tomiyama, M., Wakabayashi, K., Kanematsu, T., Hirata, M., Kaneko, S., Ueno, S., and Okada, M.
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Journal Title
J. Pharmacol. Exp. Ther
Volume: 340
Pages: 520-528
Related Report
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[Journal Article] Involvement of PRIP, phospholipase C-related but catalytically inactive protein, in bone formation2011
Author(s)
Tsutsumi, K., Matsuda, M., Kotani, M., Mizokami, A., Murakami, A., Takahashi, I., Terada, Y., Kanematsu, T., Fukami, K., Takenawa, T., Jimi, E. and Hirata, M.
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 31032-31042
Related Report
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[Journal Article] Phenotypes of pain behavior in phospholipase C-related but catalytically inactive protein type 1 knockout mice2011
Author(s)
Migita, K., Tomiyama, M., Yamada, J., Fukuzawa, M., Kanematsu, T., Hirata, M., and Ueno, S.
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Journal Title
Mol. Pain
Volume: 7
Pages: 79-79
Related Report
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[Journal Article] Dual signaling pathways of arterial constriction by extracellular UTP in the rat2011
Author(s)
Sugihara, M., Morita, H., Matsuda, M., Umebayashi, H., Kajioka, S., Ito, S., Nishida, M., Inoue, Ryosuke., Futatsuki, T., Yamazaki, J., Mori, Y., Inoue, Ryuji., Ito, Y., Abe, K. and Hirata, M.
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Journal Title
J. Pharmacol. Sci
Volume: 115
Pages: 293-305
Related Report
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[Journal Article] Dual Signaling Pathways of Arterial Constriction by Extracellular Uridine 5'-Triphosphate in the Rat2011
Author(s)
Sugihara M, Morita H, Matsuda M, Umebayashi H, Kajioka S, Ito S, Nishida M, Inoue R, Futatsuki T, Yamazaki J, Mori Y, Inoue R, Ito Y, Abe K, Hirata M
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Journal Title
Journal of Pharmacological Sciences
Volume: 115
Issue: 3
Pages: 293-308
DOI
NAID
ISSN
1347-8613, 1347-8648
Related Report
Peer Reviewed
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[Journal Article] Phospholipase C-related but catalytically inactive protein is required for insulininduced cell surface expression of・-aminobutyric acid type A receptors2010
Author(s)
Fujii, M., Kanematsu, T., Ishibashi, H., Fukami, K., Takenawa, T., Nakayama, K. I., Moss, S. J., Nabekura, J., Hirata, M.
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 4837-4846
Related Report
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[Journal Article] GABAA receptor subunit alteration-dependent diazepam insensitivity in the cerebellum of phospholipase C-related inactive protein knockout mice2010
Author(s)
Mizokami, A., Tanaka, H., Ishibashi, H., Umebayashi, H., Fukami, K., Takenawa, T., Nakayama, K. I., Yokoyama, T., Nabekura, J., Kanematsu, T. and Hirata, M.
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Journal Title
J. Neurochem
Volume: 114
Pages: 302-310
Related Report
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[Journal Article] Genetic reduction of GABAA receptorγ2 subunit expression potentiates the immobilizing action of isoflurane2010
Author(s)
Seo, K., Seino, H., Yoshikawa, H., Petrenko, A. B., Baba, H., Fujiwara, N., Someya, G., Kawano, Y., Maeda, T., Matsuda, M., Kanematsu, T. and Hirata, M.
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Journal Title
Neurosci. Lett
Volume: 472
Pages: 1-4
Related Report
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[Journal Article] Dictyostelium differentiation-inducing factor-1 binds to mitochondrial malate dehydrogenase and inhibits its activity2010
Author(s)
Matsuda, T., Takahashi-Yanaga, F., Yoshihara, T., Maenaka, K., Watanabe, Y., Miwa, Y., Morimoto, S., Kubohara, Y., Hirata, M. and Sasaguri, T.
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Journal Title
J. Pharmacol. Sci
Volume: 112
Pages: 320-326
NAID
Related Report
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[Journal Article] Assay of dense-core vesicle exocytosis using permeabilized PC12 cells2010
Author(s)
Gao, J., Takeuchi, H., Umebayashi, H., Zhang, Z., Matsuda, M. and Hirata, M.
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Journal Title
Adv. Enz. Regul
Volume: 50
Pages: 237-246
Related Report
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[Journal Article] Orofacial movements in phospholipase C-related catalytically inactive protein-1/2 double knockout mice : Effect of the GABAergic agent diazepam and the D1 dopamine receptor agonist SKF 839592010
Author(s)
Tomiyama, T., Song, L., Kobayashi, M., Kinsella, A., Kanematsu, T., Hirata, M., Koshikawa, N. and Waddington, J. L.
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Journal Title
Synapse
Volume: 64
Pages: 714-720
Related Report
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[Journal Article] Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway2010
Author(s)
Yoshihara, T., Takahashi-Yanaga, F., Shiraishi, F., Morimoto, S., Watanabe, Y., Hirata, M., Hoka, S. and Sasaguri, T.
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Journal Title
Mol. Cancer
Volume: 9
Pages: 245-245
Related Report
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[Journal Article] Binding of phospholipase C-related but catalytically inactive protein to phosphatidylinositol 4, 5-bisphosphate via the PH domain2009
Author(s)
Gao, J., Takeuchi, H., Zhao, Z., Fujii, M., Kanematsu, T. and Hirata, M.
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Journal Title
Cell. Signal
Volume: 21
Pages: 1180-1186
Related Report
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[Journal Article] Involvement of phospholipase C-related inactive protein in the mouse reproductive system through the regulation of gonadotropin levels2009
Author(s)
Matsuda, M., Tsutsumi, K., Kanematsu, T., Fukami, K., Terada, Y., Takenawa, T., Nakayama, K. I. and Hirata, M.
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Journal Title
Biol. Reprod
Volume: 81
Pages: 681-689
Related Report
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[Presentation] Involvement of PRIP in bone formation2011
Author(s)
Hirata, M., Tsutsumi, K., Kotani, M., Mizokami, A., and Matsuda M
Organizer
The 52^<nd> International Symposium on Regulation of Enzyme Activity and Synthesis in Normal and Neoplastic Tissues
Place of Presentation
Bologna
Related Report
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[Presentation] Phosphoregulation of exocytosis by PRIP2011
Author(s)
Takeuchi, H., Gao, J., Zhang, Z., Sugiyama, G., Nagano, K., and Hirata, M.
Organizer
The 10^<th> JBS Biofrontier Symposium International Symposium on New Aspects of Phospholipid Biology and Medicine 2011(The 100^<th> Anniversary of Kyushu University)
Place of Presentation
Fukuoka
Related Report
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[Presentation] PRIP, a new Ins(1, 4, 5) P_3 binding protein, its extension to neuroscience and beyond2010
Author(s)
Hirata, M., Gao, J., Matsuda, M., Takeuchi, H., and Kanematsu, T.
Organizer
The 2^<nd> Pohang Conference on Cellular Signaling
Place of Presentation
Pohang, Korea
Related Report
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[Presentation] Novel pathway of smooth muscle contraction by UTP in rat aortic myocyte2010
Author(s)
Morita, H., Sugihara, M., Matsuda, M., Kajioka, S., Ito, Y., Abe, K., Hirata, M.
Organizer
WorldPharma2010
Place of Presentation
Copenhagen, Denmark
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