Analysis of molecular mechanisms underlying neuronal development and neuronal maintenance

• Project/Area Number: 21300122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: SANADA Yoshikado 東京大学, 大学院・理学系研究科, 准教授 (50431896)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 神経前駆細胞 / 神経細胞移動 / 神経保護 / GPCR / AMPK / ALS / Nfil3 / 大脳新皮質 / 神経新生 / 神経細胞保護 / LKB1

Research Abstract

In the developing neocortex in mice, neural progenitor cells give rise to neurons. In the present study, I identified a novel regulatory mechanism contributing to neuronal differentiation of neural progenitor cells. Also, I identified a molecule with neuroprotective role and neuronal overexpression of the molecule in ALS mice alleviated motor neuron degeneration and delayed onset of the disease.

Research Products

• [Journal Article] LKB1-mediated spatrial control of GSK3β and adenomatous polyposis coli contributes to centrosomal forward movement and neuronal migration in the developing neocortex (2011)
  • Author(s): Naoyuki Asada
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 8852-8865
  • Peer Reviewed
• [Journal Article] LKB1-mediated spatial control of GSK3 ss and APC required for centrosomal forward movement and neuronal migration in the developing neocortex. (2010)
  • Author(s): Asada, N. and Sanada, K.
  • Journal Title: J. Neurosci Volume: 30 Pages: 8852-8865
  • Peer Reviewed
• [Journal Article] DYRK1A and glycogen synthase kinase 3 ss, a dual-kinase mechanism directing proteosomaldegradation of CRY2 for circadian timekeeping. (2010)
  • Author(s): Kurabayashi, N., Hirota, T., Sakai, M., Sanada, K. and Fukada Y.
  • Journal Title: Mol. Cell. Biol Volume: 30 Pages: 1757-1768
  • Peer Reviewed
• [Journal Article] DYRK1A and GSK-3β : A dual kinase mechanism directing proteasomal degradation of CRY2 for circadian timekeeping. (2010)
  • Author(s): KURABAYASHI Nobuhiro
  • Journal Title: Molecular and Cellular Biology 30 Pages: 1757-1768
  • Peer Reviewed
• [Journal Article] Increased anxiety in offspring reared by circadian Clock mutant mice.
  • Author(s): Koizumi, H., Kurabayashi, N., Watanabe, Y.and Sanada K.
  • Journal Title: PLoS ONE
  • Peer Reviewed
• [Presentation] G protein-coupled receptor signaling required for neurogenesis in the developing neocortex (2012)
  • Author(s): Kamon Sanada
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] 概日時計に異常を呈する母マウスによる養育が仔の情動に及ぼす影響. (2012)
  • Author(s): 小泉皓子、玉井総一、 眞田佳門
  • Organizer: 第85回日本生化学会大 会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] AMP-activated protein kinase regulates neurnal migration in the developing neocortex. (2012)
  • Author(s): Yasuki Naito, Kamon Sanada
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] 筋萎縮性側索硬化症モデルマウスにおける Nfil3 の神経保護作用 (2012)
  • Author(s): 倉林伸博、玉井総一、 眞田佳門
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-15
• [Presentation] Role of AMP-activated protein kinase in neuronal migration in the developing neocortex. International Symposium (2012)
  • Author(s): Naito Yasuki, Naoyuki Asada, Kamon Sanada
  • Organizer: Neocortical Organization
  • Place of Presentation: 岡崎国際会議場(愛知県)
  • Year and Date: 2012-03-12
• [Presentation] Centrosomal forward movement in migrating neocortical neurons is mediated byLKB1-GSK3beta-APC pathway. (2011)
  • Author(s): Kamon Sanada、Naoyuki Asada, Yasuki Naito
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-16
• [Presentation] Role of polarity protein LKB1 in neuronal migration. (2011)
  • Author(s): Kamon Sanada
  • Organizer: 第34回神経科学大会
  • Place of Presentation: パシフィ コ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] Role of polarity protein LKB1 in neuronal migration. (2011)
  • Author(s): Kamon Sanada
  • Organizer: 第34回神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] Centrosomal forward movement in migrating neocortical neurons is mediated by LKB1-GSK3beta-APC pathway. (2011)
  • Author(s): Kamon Sanada
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] LKB1-mediated control of GSK3 ss and APCregulates centrosomal forward movement and neuronal migration (2010)
  • Author(s): Naoyuki Asada, Kamon Sanada
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2010-11-14
• [Presentation] LKB1-mediated control of GSK3β and APC regulates centroso mal forward movement and neuronal migration (2010)
  • Author(s): Naoyuki Asada
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego Convention Cen ter (USA)
  • Year and Date: 2010-11-14
• [Presentation] 神経細胞移動においてLKB1-GSK3β-APCシグナリングは中心体の前方移動を制御する (2010)
  • Author(s): Naoyuki Asada
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-03
• [Presentation] 神経細胞移 動において LKB1-GSK3β-APC シグナリング は中心体の前方移動を制御する (2010)
  • Author(s): Naoyuki Asada, Kamon Sanada
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
• [Presentation] G protein-coupled receptor signaling required for neurogenesis in the developing neocortex.
  • Author(s): Kamon Sanada
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] 概日時計に異常を呈する母マウスによる養育が仔の情動に及ぼす影響.
  • Author(s): 小泉皓子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県)
• [Presentation] AMP-activated protein kinase regulates neurnal migration in the developing neocortex.
  • Author(s): Yasuki Naito
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県)
• [Presentation] 筋萎縮性側索硬化症モデルマウスにおけるNfil3の神経保護作用.
  • Author(s): 倉林伸博
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県)
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/mgrl/