In vivo whole-cell recording analysis of response properties of vestibular nucleus neuron to head rotation

• Project/Area Number: 21300147
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Gunma University
• Principal Investigator: SAITO Yasuhiko 群馬大学, 大学院・医学系研究科, 准教授 (70290913)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 前庭神経核 / 眼球運動 / 膜特性 / パッチクランプ / 舌下神経前位核 / ニューロン / シナプス / 前庭動眼反射 / ラット / 求心性入力

Research Abstract

We investigated the response properties of neurons in the medial vestibular nucleus (MVN) to head rotations using whole-cell recordings in in vivo preparations of rats. Most MVN neurons tested increased the firing frequency in response to the head rotation to the recording side. Furthermore, the neurons showed an afterhyperpolarization with dual components and a repetitive firing pattern with relatively constant interspike intervals. This finding suggests that MVN neurons that show specific intrinsic properties respond to the head rotation to a specific direction.

Research Products

• [Journal Article] Characterization of ionic channels underlying the specific firing pattern of a novel neuronal subtype in the rat prepositus hypoglossi nucleus (2012)
  • Author(s): Saito Y, Shino M, Yanagawa Y
  • Journal Title: Neuroscience Research Volume: 73 Pages: 32-41
  • Peer Reviewed
• [Journal Article] Electrophysiological characteristics of inhibitory neurons of the prepositus hypoglossi nucleus as analyzed in Venus-expressing transgenic rats (2011)
  • Author(s): Shino M, Kaneko R, Yanagawa Y, Kawaguchi Y, Saito Y
  • Journal Title: Neuroscience Volume: 197 Pages: 89-98
  • Peer Reviewed
• [Journal Article] Synaptic mechanism for the sustained activation of oculomotor integrator circuits in the rat prepositus hypoglossi nucleus : Contribution of Ca^<2+>-permeable AMPA receptors (2010)
  • Author(s): Saito Y. Yanagawa Y
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 15735-15746
  • Peer Reviewed
• [Journal Article] Synaptic mechanism for the sustained activation of oculomotor integrator circuits in the rat prepositus hypoglossi nucleus : Contributi on of Ca^<2+>-permeable AMPA receptors. (2010)
  • Author(s): Saito Y, Yanagawa Y
  • Journal Title: Journal of Neuroscience Volume: 30 Pages: 15735-15746
  • Peer Reviewed
• [Journal Article] Fluorescent labeling of both GABAergic and glycinergic neurons in VGAT-Venus transgenic mouse (2009)
  • Author(s): Wang Y., Kakizaki T., Sakagami H., Saito K., Ebihara S., Kato M., Hirabayashi M., Saito Y., Furuya N., Yanagawa Y
  • Journal Title: Neuroscience Volume: 164 Pages: 1031-1043
  • Peer Reviewed
• [Presentation] Control of spontaneous firings by Ca^<2+>-activated ion channels triggered by the Ca^<2+> release via ryanodine receptors in inhibitory neurons in the rat prepositus hypoglossi nucleus (2011)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: 第2回国際放射線生物学研究集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-12-03
• [Presentation] Activation of ryanodine receptors control spontaneous discharge of inhibitory neurons in the rat prepositus hypoglossi nucleus (2011)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: 北米神経科学学会
  • Place of Presentation: Washington D.C.(USA)
  • Year and Date: 2011-11-16
• [Presentation] Activation of ryanodine receptors control spontaneous discharge of inhibitory neurons in the rat prepositus hypoglossi nucleus (2011)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: 北米神経科学学会
  • Place of Presentation: Washington D.C.
  • Year and Date: 2011-11-16
• [Presentation] Spontaneous outward currents expressed preferentially in inhibitory neurons in the rat prepositus hypoglossi nucleus (2011)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] Contribution of Ca^<2+>-permeable AMPA receptors to sustained activity in rat preoositus hypoglossi nucleus neurons. (2011)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: 第1回放射線生物学研究集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-01-29
• [Presentation] Electrophysiological properties of inhibitory neurons in prepositus hypoglossi nucleus in the vesicular GABA transporter (VGAT)-Venus transgenic rats (2010)
  • Author(s): Shino M, Kaneko R, Yanagawa Y, Saito Y
  • Organizer: XVIIIth International Workshop on Genetic Systems in the Rat
  • Place of Presentation: 京都
  • Year and Date: 2010-12-01
• [Presentation] Expression of Ca^<2+>-permeable AMPA receptors in rat prepositus hypoglossi nucleus neurons (2010)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] Expression of Ca^<2+>-permeable AMPA receptors in rat prepositus hypoalossi nucleus neurons. (2010)
  • Author(s): Saito Y, Yanagawa Y
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] Local excitatory network in rat prepositus hypoglossi nucleus (2009)
  • Author(s): Saito Y, Shino M, Yanagawa Y
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Contribution of Ca^<2+>-permeable AMPA receptor to activation of excitatory network in prepositus hypoglossi nucleus (2009)
  • Author(s): Saito Y, Shino M, Yanagawa Y
  • Organizer: XXXIV International Congress of Physiological Sciences (IUPS 2009)
  • Place of Presentation: 京都
  • Year and Date: 2009-07-29
• [Remarks]
  • URL: http://genbehavneuro.dept.med.gunma-u.ac.jp/Yanagawa_Lab/Home.html