Elucidation of MHC polymorphism information in cynomolgus macaque(Macaca Fascicularis)
| Project/Area Number |
21300155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Asako 東海大学, 医学部, 准教授 (40101935)
TORII Ryuzo 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
TSUCHIYA Hideaki 滋賀医科大学, 動物生命科学研究センター, 助教 (10378440)
ITOH Yasushi 滋賀医科大学, 医学部, 准教授 (90324566)
OGASAWARA Kazumasa 滋賀医科大学, 医学部, 教授 (20169163)
HOSOMICHI Kazuyoshi 国立遺伝学研究所, 総合遺伝研究系人類遺伝研究部門, 助教 (50420948)
OTA Masao 信州大学, 医学部, 准教授 (50115333)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 遺伝育種 / カニクイザル / MHC / 再生医療 / 多型 / 薬剤感受性 |
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| Research Abstract |
We developed a genotyping methods on Macaca fascicularis(Mafa) class I and class II genes by Sanger and next generation sequencing. Using the methods we discovered new MHC alleles, and estimated MHC haplotypes in Filipino population from genotyping of the 200 animals. Moreover, we characterized five homozygous and 43 heterozygous animals that have the most frequent MHC haplotype and 23 homozygous animals that have the other MHC haplotypes from a large scale screening of 1222 animals. In summary, we characterized the Macaca fascicularis MHC polymorphisms and constructed research base for medical research in this research.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Memory Immune Responses Against Pandemic 1(H1N1) 2009 Influenza Virus Induced by a Whole Particle Vaccine in Cynomolgus Monkeys Carrying Mafa-A1 052 : 022012
Author(s)
Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Establishment of exome sequencing of cynomolgus macaque(Macaca fascicularis)2011
Author(s)
Suzuki S, Hosomichi K, Otabe K, Sakamoto K, Kurata M, Nomura M, Yamanaka H, Nakagawa H, Ota M, Inoko H, Inoue I, Shiina T
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Journal Title
DNA Polymorphism
Volume: 19
Pages: 230-234
Related Report
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[Journal Article] Analysis of MHC class II gene polymorphisms(Mafa-DR-DQ genes) in Cynomolgus Macaques(Macaca fascicularis) originated from Philippine islands2011
Author(s)
Ota M, Tanaka T, Katsuyama Y, Kita Y, Otabe K, Sakamoto K, Kurata M, Nomura M, Yamanaka H, Nakagawa H, Asamura H, Inoko H, Shiina T
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Journal Title
DNA Polymorphism
Volume: 19
Pages: 47-49
NAID
Related Report
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[Journal Article] Comparative genome analysis of the major histocompatibility complex (MHC) class i B/C segments in primates elucidated by genomic sequencing in common marmoset (Callithrix jacchus)2011
Author(s)
Shiina, T., Kono, A., Westphal, N., Suzuki, S., Hosomichi, K., Kita, Y.F, Roos, et al
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Journal Title
Immunogenetics
Volume: 63:8
Issue: 8
Pages: 485-499
DOI
Related Report
Peer Reviewed
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[Journal Article] Mitochondrial DNA diversity among three subpopulations of cynomolgus macaques(Macaca fascicularis) originating from the Indochinese region2010
Author(s)
Shiina T, Tanaka K, Katsuyama Y, Otabe K, Sakamoto K, Kuratani M, Nomura M, Yamanaka H, Nakagawa H, Inoko H, Ota M
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Journal Title
Exp. Anim
Volume: 59
Pages: 567-578
NAID
Related Report
Peer Reviewed
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[Journal Article] MHC class I A loci polymorphism and diversity in three Southeast Asian populations of cynomolgus macaque2009
Author(s)
Kita YF, Hosomichi K, Kohara S, Itoh Y, Ogasawara K, Tsuchiya H, Torii R, Inoko H, Blancher A, Kulski JK, Shiina T
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Journal Title
Immunogenetics
Volume: 61
Pages: 635-648
Related Report
Peer Reviewed
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[Journal Article] Nomenclature report on the major histocompatibility complexes, genes, and alleles of non-human primate species
Author(s)
Groot NG, Otting N, Robinson J, Blancher A, Lafont BA, Marsh SGE, 0' Connor DH, Shiina T, Walter L, Watkins DI, Bontrop RE
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Journal Title
Immunogenetics
Volume: (In press)
Related Report
Peer Reviewed
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