| Project/Area Number |
21300170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Nagasaki University |
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Principal Investigator |
KAGAWA Akio 長崎大学, 工学研究科, 教授 (00093401)
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| Co-Investigator(Kenkyū-buntansha) |
TAGAWA Yutaka 長崎大学, 医歯薬学総合研究科, 教授 (20206907)
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| Co-Investigator(Renkei-kenkyūsha) |
OHGAI Takeshi 長崎大学, 工学研究科, 准教授 (60253481)
MIZUMOTO Masayuki 長崎大学,岩手大学, 工学部,工学部, 助教(H21),准教授(H22-23) (90325671)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 金属物性 / 細胞・組織 / 水素 / 癌 / 水素貯蔵合金 / パラジウム / ニッケル / 正常細胞 / 癌細胞 / 水素ラジカル / スーパーオキシド |
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| Research Abstract |
To develop a pin-point treatment of cancer cells, hydrogen discharging behavior of hydrogen storage alloy(HSA) and the effect of hydrogen discharged from the HSA with different alloy composition and sample shape on biological cells have been studied. The results obtained are summarized as follows. 1) The amount of gaseous hydrogen and hydrogen ions as well as hydrogen radicals varied depending upon alloy composition. It is, therefore, possible to control the hydrogen discharging behavior by adjusting the alloy composition. 2) From the observation of the probability-of-survival(POS) measurement, a reduction in the POS was observed in cancer cells and a part of normal cell, and it was found that the influence of discharged hydrogen depends on the kind of cells. 3) A selective cancer cell death was observed in the region of approximately 2~ 2. 5 mm outside the samples, independent of sample shape. 4) From the ESR experiment and the detection of hydrogen peroxide using fluorescent regent, hydrogen radical, hydroxyl radical and hydrogen peroxide were detected and it was suggested that these hydrogen species would affect to cancer cell death.
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