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Research on specification conditions of the ultrasonic stimulation which promote the regeneration of the cartilage using intracellular signaling analysis

Research Project

Project/Area Number 21300171
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKanagawa Dental College (2011)
Yokohama City University (2009-2010)

Principal Investigator

TAKEUCHI Ryohei  神奈川歯科大学, 歯学部, 特任教授 (30236442)

Co-Investigator(Kenkyū-buntansha) TAKAGAKI Yuko  神奈川歯科大学, 歯学部, 教授 (60050689)
MORISHITA Shin  横浜国立大学, 環境情報工学院, 教授 (80166404)
青田 洋一  横浜国立大学, 医学部, 准教授 (40363824)
熊谷 研  横浜国立大学, 医学部, 准教授 (10468176)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords人工臓器工学 / 再生医工学 / 培養軟骨 / collagen IX / アグリカン / collagen II / 低出力パルス超音波 / 変形性関節症 / 低出力超音波 / 軟骨 / Type IIコラーゲン / Type IXコラーゲン / PCR / Tpe IIコラーゲン / Tpe IXコラーゲン
Research Abstract

The purpose of this study is to clarify the influence of changing repetition frequency(RF) and intensity(Po) of Low-Intensity Pulsed Ultrasound(LIPUS) for cultured bovine articular chondrocytes. Chondrocytes were obtained from the metatarso-phalangeal joints of bovine and were cultured. To analyze the effect of RF and Po, LIPUS was exposed at RF of 50, 500, 1000Hz and at intensity of 75, 150, 300 mW/cm^2| SATP. LIPUS stimulation for 20 minutes every day was applied to chondrocytes after 24 hours in culture. At the time of 7 days after culture, mRNA levels of Aggrecan(AGC), Type II collagen(COL2), and Type IX collagen(COL9) were evaluated by Real-time RT-PCR. In intensity, condition of 150 mW/cm^2| SATP, the RF of 500Hz increased expression of mRNA for AGC by 168% of control(p<0.05). The condition of RF of 50Hz increased expression of mRNA for COL9 by 158% of control(p<0.01). RF was set to 1000 Hz and intensity was changed, Po-300 mW/cm^2 increased expression of mRNA for COL9 by 147% of control(p<0.05). These results suggested that changing of RF of LIPUS may affect expression of each mRNA of protein in the cell. In addition, the changing of intensity and RF of LIPUS may influence to some products produced by chondrocytes during culture.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2012 2011 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] 低出力パルス超音波の繰り返し周波数および出力強度の変化が軟骨細胞に与える影響2012

    • Author(s)
      鈴木良啓, 竹内良平, 高垣裕子, 白石俊彦, 福井厚子, 森下信
    • Journal Title

      日本足の外科学会誌

      Volume: Vol.33(1)(in press)

    • Related Report
      2011 Final Research Report
  • [Journal Article] 低出力パルス超音波の繰り返し周波数および出力強度の変化が軟骨細胞に与える影響2012

    • Author(s)
      鈴木良啓, 竹内良平, 他
    • Journal Title

      日本足の外科学会誌

      Volume: 33(1)(In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 骨髄液吸着β-TCPブロックに対する低出力超音波の骨形成能に関する影響2011

    • Author(s)
      鈴木良啓
    • Organizer
      第14回超音波骨折治療研究会
    • Place of Presentation
      東京カンファレンスセンター(東京)
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Influence of the difference in repetition frequency and intensity of low intensity pulsed ultrasound for cultured chondrocytes2011

    • Author(s)
      Y. Suzuki, R. Takeuchi, Y. M. Takagaki, T. Shiraishi, A. Fukui, S. Morishita
    • Organizer
      OARSI
    • Place of Presentation
      Sandiego, USA
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 低出力パルス超音波の繰り返し周波数および出力強度の変化が軟骨細胞に与える影響2011

    • Author(s)
      鈴木良啓, 竹内良平, 高垣裕子, 白石俊彦, 福井厚子, 森下信
    • Organizer
      第38回日本足の外科学会
    • Place of Presentation
      奈良
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 低出力パルス超音波の繰り返し周波数および出力強度の変化が軟骨細胞に与える影響2010

    • Author(s)
      鈴木良啓
    • Organizer
      京都・奈良・横浜バイオメカニクスカンファレンス
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 軟骨細胞に対する低出力超音波刺激の影響2009

    • Author(s)
      鈴木良啓
    • Organizer
      京都・横浜カンファレンス
    • Place of Presentation
      京都大学
    • Year and Date
      2009-12-19
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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