| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
To achieve efficient and durable transgene expression by controlled intranuclear disposition, we inserted DNA sequences that modulate binding to histones into plasmid DNAs, introduced histone-plasmid DNA complexes into cells, and developed a plasmid system that recruited transcription factors. We found that transgene expression was improved in efficiency and was maintained for a week. Moreover, we revealed that gene correction by tailed duplexes was a useful candidate in gene therapy.
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