Mechanisms of increase in exercise induced energy expenditure

• Project/Area Number: 21300240
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: National Institute of Health and Nutrition
• Principal Investigator: MIURA Shinji 独立行政法人国立健康・栄養研究所, 基礎栄養研究部脂質・糖代謝研究室, 室長 (10342932)
• Co-Investigator(Renkei-kenkyūsha): EZAKI Osamu 独立行政法人国立健康・栄養研究所, 基礎栄養研究部, 部長 (90191923) ; KAMEI Yasutomi 東京医科歯科大学, 難治疾患研究所, 特任教授 (70300829) ; KANO Yutaka 電気通信大学, 情報理工学部, 准教授 (90293133)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: エネルギー代謝 / AMPK / LKB1 / 横隔膜 / 筋線維 / PGC-1 / 運動 / ミトコンドリア / fuel sensor

Research Abstract

Trained subjects have a high VO2max due to a high cardiac output and high metabolic capacity of skeletal muscles. Because exercise training increases peroxisome proliferator-activated receptorγcoactivator 1α(PGC-1α) in skeletal muscle, PGC-1α-mediated changes may contribute to the improvement of exercise capacity and VO2max. There are three isoforms of PGC-1αmRNA. PGC-1α-b protein is a predominant PGC-1αisoform in response to exercise. In this study, we showed that PGC-1α-b induced increased mitochondrial biogenesis, capillaries, and fatty acid transporters in skeletal muscles may contribute to improved exercise capacity via an increase in fatty acid utilization.

Research Products

• [Journal Article] Skeletal muscle-specific expression of PGC-1α-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake (2011)
  • Author(s): Tadaishi, M., Miura, S., Kai, Y., Kano, Y., Oishi, Y., and Ezaki, O.
  • Journal Title: PLoS ONE Volume: 6(12)
  • URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028290
• [Journal Article] Control of microvascular PO2 kinetics following onset of muscle contractions : Role for AMPK (2011)
  • Author(s): Kano, Y., Poole, D. C., Sudo, M., Hirachi, T., Miura, S., and Ezaki, O.
  • Journal Title: Am. J. Physiol. Regul. Integr. Comp. Physiol Volume: 301
• [Journal Article] Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-1αmRNA : a role ofβ2-adrenergic receptor activation (2011)
  • Author(s): Tadaishi, M., Miura, S., Kai, Y., Kawasaki, E., Koshinaka, K., Kawanaka, K., Nagata, J., Oishi, Y., and Ezaki, O.
  • Journal Title: Am. J. Physiol. Endocrinol. Metab Volume: 300
• [Journal Article] Skeletal muscle-specific expression of PGC-1α-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake (2011)
  • Author(s): Tadaishi, M., Miura, S., Kai, Y., Kano, Y., Oishi, Y., Ezaki, O.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Control of microvascular PO2 kinetics following onset of muscle contractions : Role for AMPK (2011)
  • Author(s): Kano, Y., Poole, D.C., Sudo, M., Hirachi, T., Miura, S., Ezaki, O.
  • Journal Title: Am.J.Physiol.Regul.Integr.Comp.Physiol. Volume: 301
  • Peer Reviewed
• [Journal Article] Effect of exercise intensity and AICAR on isoform-specific expressions of murine skeletal muscle PGC-la mRNA : a role of f32-adrenergic receptor activation. (2011)
  • Author(s): Tadaishi, M., Miura, S., Kai, Y., Kawasaki, E., Koshinaka, K., Kawanaka, K., Nagata, J., Oishi, Y., Ezaki, O.
  • Journal Title: Am.J.Physiol.Endocrinol.Metab. Volume: 300
  • Peer Reviewed
• [Journal Article] The cathepsin L gene is a direct taret of FOXO1 in the skeletal muscle. (2010)
  • Author(s): Yamazaki, Y., Kamei, Y., Sugita, S., Akaike, F., Kanai, S., Miura, S., Hirata, Y., Troen, B.R., Kitamura, T., Nishino, I., Suganami, T., Ezaki, O., Ogawa, Y.
  • Journal Title: Biochem.J. 427 Pages: 171-178
  • Peer Reviewed
• [Journal Article] Increased expression of DNA methyltransferase 3a in obese adipose tissue : studies with transgenic mice. (2010)
  • Author(s): Kamei, Y., Suganami, T., Ehara, T., Kanai, S., Hayashi, K., Yamamoto, Y., Miura, S., Ezaki, O., Okano, M., Ogawa, Y.
  • Journal Title: Obesity 18 Pages: 314-321
  • Peer Reviewed
• [Journal Article] Ubiquitin C-terminal hydrolase-L3-knockout mice are resistant to diet-induced obesity and show increased activation of AMP-activated protein kinase in skeletal muscle (2009)
  • Author(s): Setsuie, R., Suzuki, M., Kabuta, T., Fujita, H., Miura, S., Ichihara, N., Yamada, D., Wang, YL., Ezaki, O., Suzuki, Y., and Wada, K.
  • Journal Title: FASEB J Volume: 23 Pages: 4148-4157
• [Journal Article] α2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise (2009)
  • Author(s): Miura, S., Kai, Y., Kamei, Y., Bruce, C. R., Kubota, N., Febbraio, M. A., Kadowaki, T., and Ezaki, O.
  • Journal Title: Am. J. Physiol. Endocrinol. Metab Volume: 296
• [Journal Article] Ubiquitin C-terminal hydrolase-L3-knockout mice are resistant to diet-induced obesity and show increased activation of AMP-activated protein kinase in skeletal muscle. (2009)
  • Author(s): Setsuie, R., Suzuki, M., Kabuta, T., Fujita, H., Miura, S., Ichihara, N., Yamada, D., Wang, YL., Ezaki, O., Suzuki, Y., Wada, K.
  • Journal Title: FASEB J. 23 Pages: 4148-4157
  • Peer Reviewed
• [Journal Article] Overexpression of FOXO1 in skeletal muscle does not alter longevity in mice. (2009)
  • Author(s): Chiba, T., Kamei, Y., Shimizu, T., Shirasawa, T., Katsumata, A., Shiraishi, L., Sugita, S., Ogawa, Y., Miura, S., Ezaki, O.
  • Journal Title: Mech.Ageing Dev. 130 Pages: 420-428
  • Peer Reviewed
• [Presentation] NEATの測定意義と脂肪燃焼の分子機序 (2011)
  • Author(s): 江崎治、三浦進司、野田光彦
  • Organizer: 第32回日本肥満学会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2011-09-24
• [Presentation] 骨格筋特異的PGC-1α-b過剰発現による脂肪燃焼効率の向上 (2011)
  • Author(s): 只石幹、三浦進司、江崎治
  • Organizer: 第32回日本肥満学会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2011-09-24
• [Presentation] 運動による脂肪酸酸化亢進メカニズム:骨格筋LKB1-AMPK情報伝達系の関与 (2011)
  • Author(s): 三浦進司、只石幹、江崎治
  • Organizer: 第32回日本肥満学会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2011-09-23
• [Presentation] 運動による脂肪酸酸化亢進メカニズム (2011)
  • Author(s): 三浦進司、只石幹、江崎治
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 海峡メッセ下関(山口)
  • Year and Date: 2011-09-17
• [Presentation] 運動による脂肪酸酸化亢進メカニズム (2011)
  • Author(s): 三浦進司、只石幹、江崎治
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 海峡メッセ下関(山口)
  • Year and Date: 2011-09-17
• [Presentation] 骨格筋PGC-1α-b過剰発現が運動継続能力、VO2maxに及ぼす影響 (2011)
  • Author(s): 只石幹、三浦進司、江崎治
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 海峡メッセ下関(山口)
  • Year and Date: 2011-09-16
• [Presentation] 骨格筋PGC-1α-b過剰発現が運動継続能力、VO_2maxに及ぼす影響 (2011)
  • Author(s): 只石幹、三浦進司、江崎治
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 海峡メッセ下関(山口)
  • Year and Date: 2011-09-16
• [Presentation] 骨格筋LKB1活性は低強度運動時の脂肪酸酸化亢進に必須である (2010)
  • Author(s): 三浦進司、只石幹、江崎治
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 千葉商科大学(千葉)
  • Year and Date: 2010-09-17
• [Presentation] 運動強度の違いがPGC-1αisoform mRNA発現に及ぼす影響 (2010)
  • Author(s): 只石幹、三浦進司、川中健太郎、江崎治
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 千葉商科大学(千葉)
  • Year and Date: 2010-09-16
• [Presentation] LKB1 activity in skeletal muscle is essential for an increase in fatty acid oxidation during low-intensity exercise (2010)
  • Author(s): Miura S、Kai Y、Tadaishi M、Ezaki O
  • Organizer: 70th Scientific Sessions, American Diabetes Association
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 2010-06-26
• [Presentation] LKBI activity in skeletal muscle is essential for an increase in fatty acid oxidation during low-intensity exercise. (2010)
  • Author(s): Miura S., Kai Y., Tadaishi M., Ezaki O.
  • Organizer: 70th Scientific Sessions, American Diabetes Association
  • Place of Presentation: Orland, Florida, USA
  • Year and Date: 2010-06-26
• [Presentation] 運動はどのようにして脂肪酸酸化を亢進するのか (2010)
  • Author(s): 三浦進司
  • Organizer: 日本農芸化学会2010年度大会シンポジウム(骨格筋機能と生活習慣病)
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-03-30
• [Presentation] 運動はどのようにして脂肪酸酸化を亢進するのか (2010)
  • Author(s): 三浦進司
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2010-03-30
• [Presentation] The role of AMPK in skeletal muscle on microvascular PO_2 kinetics following onset of muscle contraction (2010)
  • Author(s): Kano Y、Poole DC、Sudo M、Miura S、Ezaki O
  • Organizer: Integrative Phyisiology of Exercise
  • Place of Presentation: Miami Beach, Florida, USA
• [Presentation] 低強度運動による脂質代謝亢進に骨格筋AMPKの活性化は必須ではない (2009)
  • Author(s): 三浦進司
  • Organizer: 第64回日本体力医学会
  • Place of Presentation: 朱鷺メッセ(新潟市)
  • Year and Date: 2009-09-18
• [Presentation] 低強度運動による脂質代謝亢進に骨格筋AMPKの活性化は必須ではない (2009)
  • Author(s): 三浦進司
  • Organizer: 第64回日本体力医学会
  • Place of Presentation: 朱鷺メッセ、新潟
  • Year and Date: 2009-09-18
• [Presentation] Fibrate treatment causes muscle atrophy with increased FOXO1 mRNA in a PPAR α-dependent manner in mice. (2009)
  • Author(s): Miura, S., Kai, Y., Ezaki, O
  • Organizer: The 69th American Diabetes Association Scientific Sessions
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2009-06-06
• [Presentation] 運動による骨格筋PGC-1α新規アイソフォームの発現 (2009)
  • Author(s): 三浦進司
  • Organizer: 第1回分子骨格筋代謝研究会
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2009-03-28
• [Book] 運動療法-運動療法の理論,すべてがわかる最新・糖尿病 (2011)
  • Author(s): 江崎治、三浦進司
  • Publisher: 照林社
• [Book] 生活習慣病の栄養管理「糖分摂取過剰と生活習慣病」、成人病と生活習慣病 (2011)
  • Author(s): 三浦進司、只石幹、江崎治
• [Book] AMPキナーゼによる糖の取り込み制御、内分泌・糖尿病・代謝内科 (2011)
  • Author(s): 江崎治、三浦進司
• [Book] すべてがわかる最新・糖尿病(運動療法-運動療法の理論) (2011)
  • Author(s): 江崎治、三浦進司
  • Total Pages: 5
  • Publisher: 照林社
• [Book] PGC-1αとミトコンドリア機能、エネルギー代謝、Adiposcience (2010)
  • Author(s): 江崎治、三浦進司
• [Book] 肥満症(2) PGC-1、日本臨床増刊号 (2010)
  • Author(s): 三浦進司、只石幹、江崎治
• [Book] 肥満症(2)筋肉などでの代謝調節、日本臨床増刊号 (2010)
  • Author(s): 江崎治、千葉剛、三浦進司
• [Book] PGC-1の多彩な機能と糖・脂質代謝制御における意義-糖・脂質代謝制御におけるPGC-1αアイソフォームa, b, cの意義、内分泌・糖尿病科 (2009)
  • Author(s): 江崎治、三浦進司
• [Book] 運動の抗肥満効果、ILSI JAPAN (2009)
  • Author(s): 三浦進司、江崎治
• [Remarks]
  • URL: http://www.nih.go.jp/eiken/info/pdf/kenkoeiyonews31.pdf