Establishment of in vitro gut inflammatory model and search and mechanism of food factor possessing anti-inflammatory activit y
Project/Area Number |
21300276
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Eating habits, studies on eating habits
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Research Institution | Kobe University |
Principal Investigator |
MIZUNO Masashi 神戸大学, 大学院・農学研究科, 教授 (00212233)
|
Co-Investigator(Kenkyū-buntansha) |
NISHITANI Yosuke 神戸大学, 自然科学系先端融合研究環重点研究部, 助教 (80457093)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
|
Keywords | 健康と食生活 / 炎症性腸疾患 / マクロファージ / インターロイキン-8 / レンチナン / エンドサイトーシス / 腫瘍壊死因子 / インターナリゼイション / キノコ / プロバイオティックス / インターフェロン-γ / ポリフェノール / 乳酸菌 |
Research Abstract |
A system for assessing the anti-inflammatory effect of food factors has been developed by establishing a co-culture system with intestinal epithelial Caco-2 cells(apical side) and macrophage RAW264. 7 cells(basolateral side) stimulated with lipopolysaccharide. Curcumin and luteolin as polyphenols, fucoidan and lentinan as polysaccharide shows the anti-inflammatory activity in this system. Particularly, it was demonstrated that fucoidan indicated its activity by producing hydrogen peroxide from Caco-2 cells.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] TLR ligands of Lactobacillus sakei LK-117 isolated from seed mash for brewing sake are potent inducers of IL-122011
Author(s)
Masuda, Y., Takahashi, T., Yoshida, K., Nishitani, Y., Mizuno, M. and Mizoguchi, H.
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Journal Title
J. Biosci. Bioeng.
Volume: 112
Pages: 363-368
NAID
Related Report
Peer Reviewed
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[Journal Article] Oral treatment with extract of Agaricus blazei Murill enhanced Th1 response through intestinal epithelial cells and suppressed OVA-sensitized allergy in mice2011
Author(s)
Bouike, G., Nishitani, Y., Shiomi, H., Yoshida, M., Azuma, T., Hashimoto, T., Kanazawa, K. and Mizuno, M.
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Journal Title
Evid. Based Complement. Alternat
Volume: Volume
Issue: 1
Pages: 11-11
DOI
Related Report
Peer Reviewed
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[Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga2010
Author(s)
Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K. and Motomura, T.
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Journal Title
Silvetia babingtonii
Volume: 232
Pages: 287-298
Related Report
Peer Reviewed
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[Journal Article] Lipoxin A4 reduces lipopolysaccharide-induced inflammation in macrophages and intestinal epithelial cells through inhibition of NF-kappaB activation2010
Author(s)
Kure, I., Nishiumi, S., Nishitani, Y., Tanoue, T., Ishida, T., Mizuno, M., Fujita, T., Kutsumi, H., Arita, M., Azuma, T. and Yoshida, M.
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Journal Title
J. Pharmacol. Exp. Ther.
Volume: 332
Pages: 541-548
Related Report
Peer Reviewed
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[Journal Article] Oral treatment with extract of Agaricus blazei Murill enhanced Th1 response through intestinal epithelial cells and suppressed OVA-sensitized allergy in mice2010
Author(s)
Bouike, G., Nishitani, Y., Shiomi, H., Yoshida, M., Azuma, T., Hashimoto, T., Kanazawa, K., Mizuno, M.
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Journal Title
Evid.Based Complement. Altermat.Med.
Volume: ID532180
Pages: 1-10
Related Report
Peer Reviewed
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[Journal Article] Lactococcus lactis subsp. cremoris FC alleviates symptoms of colitis induced by dextran sulfate sodium in mice2009
Author(s)
Nishitani, Y., Tanoue, T., Yamada, K., Ishida, T., Yoshida, M., Azuma, T. and Mizuno, M.
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Journal Title
Int. Immunopharmacol.
Volume: 9
Pages: 1444-1451
Related Report
Peer Reviewed
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[Journal Article] The distribution and accumulation of fucoxanthin and its metabolites after oral-administration in mice2009
Author(s)
Hashimoto, T., Ozaki, Y., Taminato, M., Das, S. K., Mizuno, M., Yoshimura, K., Maoka, T. and Kanazawa, K.
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Journal Title
Br. J. Nutr
Volume: 102
Pages: 242-248
NAID
Related Report
Peer Reviewed
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[Journal Article] Quantitation and localization of fucoidan in Laminaria japonica using a novel antibody2009
Author(s)
Mizuno, M., Nishitani, Y., Tanoue, T., Matoba, Y., Ojima, T., Hashimoto, T. and Kanazawa, K.
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Journal Title
Biosci. Biotech. Biochem
Volume: 73
Pages: 335-338
Related Report
Peer Reviewed
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[Journal Article] Lactococcus lactis subsp.cremoris FC alleviates symptoms of colitis induced by dextran sulfate sodium in mice2009
Author(s)
Nishitani, Y., Tanoue, T., Yamada, K., Ishida, T., Yoshida, M., Azuma, T., Mizuno, M.
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Journal Title
International Immunopharmacology 9
Pages: 1444-1451
Related Report
Peer Reviewed
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[Presentation] βグルカンの機能性解明2010
Author(s)
水野雅史
Organizer
第3回 βグルカンシンポジウムin大阪
Place of Presentation
TKP大阪本町ビジネスセンター(大阪府)
Year and Date
2010-03-12
Related Report
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