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Screening of foods with anti-diabetic-complications function and elucidation of their functionality and mechanism of the function

Research Project

Project/Area Number 21300278
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionKochi University

Principal Investigator

YAGI Toshiharu  高知大学, 教育研究部・自然科学系, 教授 (90110759)

Co-Investigator(Kenkyū-buntansha) TODA Katsumi  高知大学, 教育研究部・医療学系, 准教授 (40197893)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords健康と食生活 / ピリドキサミン / 糖尿病合併症 / 糖新生 / 食品 / ビタミンB6 / 抗糖尿病合併症
Research Abstract

Pyridoxamine(PM), one of vitamin B_6, has anti-diabetic-complications function. Foods containing a lot of PM, thus, may have this function. At first, a novel determination method of PM in foods has been developed. PM contents in several foods were determined. Then, PM was supplied to mice, and expression levels of genes encoding gluconeogenesis-related proteins. PM increased gluconeogenesis in the mice.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2012 2011 2010 2009 Other

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (16 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Abnormality in expression levels of gluconeogenesis-related genes by high-dose supplementation with pyridoxamine in mice2012

    • Author(s)
      H. T. V. Do, K. Toda, T. Saibara, T. Yagi
    • Journal Title

      International Journal for Vitamin and Nutrition Research

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B_6 in foods2012

    • Author(s)
      H. T. V. Do, Y. Ide, A. N. Mugo, T. Yagi
    • Journal Title

      Food and Nutrition Research

      Volume: 55 Pages: 5409-5416

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Individual vitamin B_6 contents in selected Japanese sushi toppings2012

    • Author(s)
      H. T. V. Do, T. Yagi
    • Journal Title

      International Journal of Food Sciences and Nutrition

      Volume: 63(2) Pages: 246-249

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase2012

    • Author(s)
      Mugo, A. N., Kobayashi, J., Mikami, B., Ohnishi, K., Yagi T.
    • Journal Title

      the first enzyme in pyridoxine degradation pathway I. Acta Crystallographica. Section F

      Volume: 68(1) Pages: 66-68

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ビタミンB_6測定法の現状と課題(総説)2011

    • Author(s)
      八木年晴
    • Journal Title

      ビタミン

      Volume: 85(3) Pages: 116-126

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti2011

    • Author(s)
      Chu, H. N., Kobayashi, J., Mikami, B., Yagi, T.
    • Journal Title

      Bioscience Biotechnology and Biochemistry

      Volume: 75(2) Pages: 388-390

    • NAID

      10027898248

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods2011

    • Author(s)
      Do, H.T.V., Y.Ide, Mugo, A.N., Yagi, T.
    • Journal Title

      Food & Nutrition Research

      Volume: 55 Pages: 5409-5416

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I2011

    • Author(s)
      Mugo A.N., Kobayashi J., Mikami B., Ohnishi K., Yagi T.
    • Journal Title

      Acta Crystallographica Section F

      Volume: 68 Issue: 1 Pages: 66-68

    • DOI

      10.1107/s1744309111048688

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Crystal Structure of SDR-Type Pyridoxal 4-Dehydrogenase of Mesorhizobium loti2011

    • Author(s)
      Chu, H.N., Kobayashi, J., Mikami, B., Yagi, T.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 2 Pages: 388-390

    • DOI

      10.1271/bbb.100748

    • NAID

      10027898248

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ビタミンB6測定法の現状と課題2011

    • Author(s)
      八木年晴
    • Journal Title

      ビタミン

      Volume: 85 Pages: 116-126

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of simultaneous enzymatic assay method for all six individual vitamin B_6 forms and pyridoxine-β-glucoside2010

    • Author(s)
      Yagi, T., Murayama, R., Do, H. T. V., Ide, Y., Mugo, A. M., Yoshikane, Y.
    • Journal Title

      J. Nutr. Sci. Vitaminol

      Volume: 56(3) Pages: 157-163

    • NAID

      10030703710

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti2010

    • Author(s)
      Chu, H. N., Kobayashi, J., Yoshikane, Y., Mikami, B., Yagi, T.
    • Journal Title

      Acta Crystallographica Section F

      Volume: 66(6) Pages: 718-720

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells.2010

    • Author(s)
      Ge F., Yagi, T
    • Journal Title

      J.Biochem.

      Volume: 147 Pages: 291-295

    • NAID

      10027874097

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with 1-glutamate as an amino donor2010

    • Author(s)
      Yoshikane, Y., Tamura, A., Yokochi, N., Ellouze, K., Yamamura, E., Mizunaga, H., Fujimoto, N., (…), Yagi, T.
    • Journal Title

      J.Mol.Cat.Enz.

      Volume: 67 Pages: 104-110

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of simultaneous enzymatic assay method for all six individual vitamin B6 forms and pyridoxine-β-glucoside.2010

    • Author(s)
      Yagi, T., Murayama, R., Do, H.T.V., Ide, Y., Mugo, A.M., Yoshikane, Y.
    • Journal Title

      J.Nutr.Sci.Vitaminol.

      Volume: 56 Pages: 157-163

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti.2010

    • Author(s)
      Chu, H.N., Kobayashi, J., Yoshikane, Y., Mikami, B., Yagi, T.
    • Journal Title

      Aca.Cryst.Sec.F

      Volume: 66 Pages: 718-720

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Esmond E.Snell-The pathfinder of B vitamins and cofactors.2010

    • Author(s)
      Hayashi, H., Tanase, S., Yagi, T.
    • Journal Title

      J.Biochem.

      Volume: 147 Pages: 451-457

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of simultaneous enzymatic assay method for all of the six individual vitamin B6 and pyridoxine-β-glucoside2010

    • Author(s)
      Yagi, T, Ryutaro Murayama, Huong Do Thi Viet, Youhei Ide, Andrew Njagi Mugo, Yu Yoshikane
    • Journal Title

      J.Nutr.Sci.Vitaminol. 56(In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells2010

    • Author(s)
      Ge, F., Yagi T.
    • Journal Title

      J.Biochem. 147

      Pages: 291-295

    • NAID

      10027874097

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of 4-Pyridoxolactonase from Mesorhizobium loti2009

    • Author(s)
      Matsuda, S., Yokochi, N., Yoshikane, Y., Kobayashi, J., Huy, C. N., Baba, S., Kuramitsu, S., (...), Yagi, T.
    • Journal Title

      Acta Crystal. Sect

      Volume: F 65(9) Pages: 886-889

    • NAID

      120001671533

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway i of pyridoxine2009

    • Author(s)
      Kobayashi, J., Yoshida, H., Chu, H. N., Yoshikane, Y., Kamitori, S., Yagi, T.
    • Journal Title

      Acta Crystal. Se

      Volume: F 65(8) Pages: 829-831

    • NAID

      120001671534

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase2009

    • Author(s)
      Yokochi, N., Yoshikane, Y., Matsumoto, S., Fujisawa, M., Ohnishi, K., Yagi, T.
    • Journal Title

      J. Biochem

      Volume: 145(4) Pages: 493-503

    • NAID

      10026302164

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase2009

    • Author(s)
      Yokochi, N., Yoshikane, Y., Matsumoto, S., Fujisawa, M., Ohnishi, K., Yagi, T.
    • Journal Title

      J.Biochem. 145

      Pages: 493-503

    • NAID

      10026302164

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of 4-Pyridoxolactonase from Mesorhizobium loti2009

    • Author(s)
      Matsuda, S., Yokochi, N., Yoshikane, Y., Kobayashi, J., Huy, C.N., Baba, S., Kuramitsu, S., Yagi, T.
    • Journal Title

      Acta Cryst.F 65

      Pages: 886-889

    • NAID

      120001671533

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway i of pyridoxine2009

    • Author(s)
      Kobayashi, J., Yoshida, H., Chu, H.N., Yoshikane, Y., Kamitori, S., Yagi, T.
    • Journal Title

      Acta Cryst.F. 65

      Pages: 829-831

    • NAID

      120001671534

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 牛乳と人乳中の各種ビタミンB_6化合物含有量について2011

    • Author(s)
      八木年晴、岸貴之、井手陽平、Do Thi Viet Huong
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2011-06-05
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト尿中ビタミンB6分別定量法2010

    • Author(s)
      村山隆太郎, Do Thi Viet Huong, 井手陽平, 吉金優, 八木年晴
    • Organizer
      日本農芸化学会中四国支部例会
    • Place of Presentation
      愛媛大学(松山)
    • Year and Date
      2010-01-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 膜酵素4-ピリドキシン酸デヒドロゲナーゼのCoQ結合部位2010

    • Author(s)
      鈴木太郎, 葛飛, 小林淳, 吉金優, 八木年晴
    • Organizer
      日本農芸化学会中四国支部例会
    • Place of Presentation
      愛媛大学(松山)
    • Year and Date
      2010-01-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Contents of six natural vitamin B_6 and pyridoxine-β-glucoside in some foods determined by all-enzymatic HPLC method2010

    • Author(s)
      Huong Do Thi Viet, Youhei Ide, Ryutaro Murayama, Andrew Njagi Mugo, Toshiharu Yagi.
    • Organizer
      日本ビタミン学会第62回大会
    • Place of Presentation
      岩手県民情報交流センター
    • Related Report
      2011 Final Research Report
  • [Presentation] ビタミンB_6分解酵素4-pyrodoxolactonaseのX線結晶構造解析2010

    • Author(s)
      小林淳、吉金優、馬場清喜、三上文三、八木年晴
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2011 Final Research Report
  • [Presentation] Crystal structure of SDR-type pyridoxal 4-dehydrogenase from Mesorhizobium loti2010

    • Author(s)
      Huy Nhat Chu, Jun Kobayashi, Yu Yoshikane, Toshiharu Yagi, Bunzo Mikami
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2011 Final Research Report
  • [Presentation] All-enzymatic HPLC method for determination of individual and total contents of vitamin B_6 in foods2010

    • Author(s)
      Toshiharu Yagi
    • Organizer
      The first international vitamin conference(Copenhagen)
    • Place of Presentation
      IDA Conference Center, Copenhagen, Denmark
    • Related Report
      2011 Final Research Report
  • [Presentation] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods.2010

    • Author(s)
      Toshiharu Yagi
    • Organizer
      The first international vitamin conference(Copenhagen)
    • Place of Presentation
      コペンハーゲン
    • Related Report
      2010 Annual Research Report
  • [Presentation] Contents of six natural vitamin B6 and pyridoxine-β-glucoside in some foods determined by all-enzymatic HPLC method..2010

    • Author(s)
      Huong Do Thi Viet, Youhei Ide, Ryutaro Murayama, Andrew Njagi Mugo, Toshiharu Yagi.
    • Organizer
      日本ビタミン学会第62回大会
    • Place of Presentation
      岩手県民情報交流センター
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビタミンB6分解酵素4-pyrodoxolactonaseのX線結晶構造解析2010

    • Author(s)
      小林淳、吉金優、馬場清喜、三上文三、八木年晴
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Crystal Structure of SDR-type pyridoxal 4-dehydrogenase from Mesorhizobium loti2010

    • Author(s)
      Huy Nhat Chu, Jun Kobayashi, Yu Yoshikane, Toshiharu Yagi, Bunzo Mikami
    • Organizer
      日本生化学会第83回大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高圧塩酸処理を行わない酵素-HPLC法による食品中ビタミンB6分別定量2009

    • Author(s)
      井手陽平, 村山隆太郎, Do Thi Viet Huong, 吉金優, 八木年晴
    • Organizer
      日本農芸化学会関西・中四国・西日本支部合同大会
    • Place of Presentation
      琉球大学(沖縄)
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Topology Structure and Biochemical Function of 4-Pyridoxic Acid Dehydrogenase in Bacteria2009

    • Author(s)
      Ge Fei, Yu Yoshikane, Kouhei Ohnishi, Toshiharu Yagi
    • Organizer
      21stIUBMB & 12^<th> FAOBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      国際会議場(上海)
    • Year and Date
      2009-08-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] PLP、PMP、PNP、PNGの酵素HPLC法による定量条件の改良II2009

    • Author(s)
      井手陽平、小林淳、村山隆太郎、吉金優、八木年晴
    • Organizer
      ビタミン学会大会
    • Place of Presentation
      亀岡
    • Year and Date
      2009-05-31
    • Related Report
      2011 Final Research Report
  • [Presentation] PLP、PMP、PNP、PNGの酵素HPLC法による定量条件の改良II2009

    • Author(s)
      井手陽平, 小林淳, 村山隆太郎, 吉金優, 八木年晴
    • Organizer
      日本ビタミン学会大会
    • Place of Presentation
      京都学園大学(亀岡)
    • Year and Date
      2009-05-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] ピリドキシン分解酵素4-ピリドキシン酸デヒドロゲナーゼの膜トポロジー2009

    • Author(s)
      葛飛、吉金優、八木年晴
    • Organizer
      農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2011 Final Research Report
  • [Book] ビタミン総合辞典2010

    • Author(s)
      日本ビタミン学会
    • Total Pages
      624
    • Publisher
      朝倉書店
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.iic.kochi-u.ac.jp/ipc/research/home.htm

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] ビタミンB6の分別定量法およびビタミンB6の分別定量用キット2009

    • Inventor(s)
      八木年晴
    • Industrial Property Rights Holder
      高知大学
    • Industrial Property Number
      2009-233631
    • Filing Date
      2009-09-07
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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