Simple fractionation analysis for dissolved humic substances : utilization for DOM dynamic analysis in soil and water ecosystems.

• Project/Area Number: 21310010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: Kobe University
• Principal Investigator: FUJITAKE Nobuhide 神戸大学, 大学院・農学研究科, 教授 (50243332)
• Co-Investigator(Kenkyū-buntansha): NAGAO Seiya 金沢大学, 環日本海域環境研究センター, 教授 (20343014) ; KAWAHIGASHI Masayuki 首都大学東京, 都市環境科学研究科, 准教授 (60297794) ; TANI Masayuki 帯広畜産大学, 畜産学部, 准教授 (00271750) ; SUGIYAMA Yuko 兵庫県立大学, 環境人間学部, 准教授 (40305694) ; HIROTA Mitsuru 筑波大学, 生命環境科学研究科, 准教授 (90391151) ; TAKAHASHI Katsutoshi 独立行政法人産業技術総合研究所, 計測フロンティア研究部門, 主任研究員 (00271792)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 土壌生態 / 陸水生態 / 炭素循環 / 樹脂吸着法 / フミン物質 / 炭素動態 / 溶存有機物 / 腐植物質 / NMR分析 / DAX8樹脂 / 土壌-陸水系 / フミン物暫

Research Abstract

Fractionation methods based on the hydrophobic-and hydrophilic-properties of the DOM, which governs the carbon dynamics in soil and water ecosystems greatly, were developed as new DOM dynamics analysis. By the successive method based on resin adsorption, it found out that the DOM could classify to hydrophilic DOM and hydrophobic DOM, and the latter further to DOM of strong hydrophobic and weak hydrophobic. The usefulness of this facilitated technique was proved through actual analysis of DOM in some of the soils and the lake waters.

Research Products

• [Journal Article] Europium bind-ing abilities of dissolved humic substances isolated from deep groundwater in Horonobe area, Hokkaido, Japan (2012)
  • Author(s): Terashima, M., Nagao, S., Iwatsuki, T., Fujitake, N., Seida, Y., Iijima, K., Yoshikawa, H.
  • Journal Title: J. Nuclear Sci. Technl
  • Peer Reviewed
• [Journal Article] <13>^CNMRならびに高速サイズ排除クロマトグラフィー(HPSEC)による土壌腐植酸の特徴付け (2012)
  • Author(s): 藤嶽暢英・浅川大地・柳由貴子
  • Journal Title: 分析化学 Volume: 61 Pages: 287-294
  • Peer Reviewed
• [Journal Article] Seasonal characteristics of surface water fulvic acids from Lake Biwa and Lake Tankai in Japan (2012)
  • Author(s): Fujitake, N., Tsuda, K., Mori, H., Aso S., Kodama, H., Maruo, M., Yonebayashi, K.
  • Journal Title: Limnology Volume: 13 Pages: 45-53
  • NAID: 10030761028
  • Peer Reviewed
• [Journal Article] ^<13>CNMRならびに高速サイズ排除クロマトグラフィー(HPSEC)による土壌腐植酸の特徴付け (2012)
  • Author(s): 藤嶽暢英・浅川大地・柳由貴子
  • Journal Title: 分析化学 Volume: 61(印刷中)(掲載確定)
  • Peer Reviewed
• [Journal Article] Carbon isotope composition of dissolved humic acid and fulvic acids in the Tokachi River system (2011)
  • Author(s): Nagao, S., Kodama, H., Aramaki, T., Fujitake, N., Uchida, M., Shibata, Y.
  • Journal Title: Radiation Protection Dosimetry Volume: 146 Pages: 322-325
  • Peer Reviewed
• [Journal Article] Molecular size fractionation of soil humic acids using preparative high performance size-exclusion chromatography (2011)
  • Author(s): D.Asakawa, N.Fujitake, 他
  • Journal Title: Journal of Chromatography A Volume: 1218 Issue: 37 Pages: 6448-6453
  • DOI: 10.1016/j.chroma.2011.07.030
  • Peer Reviewed
• [Journal Article] Particle sizes of standard humic substances calculated as radii of gyration, maximum diameter and hydrodynamic radii (2011)
  • Author(s): M.Kawahigashi, N.Fujitake, 他
  • Journal Title: Humic Substances Research Volume: 8 Pages: 13-18
  • Peer Reviewed
• [Journal Article] Decolorization of soil fulvic acids by laccases from Trametes versicolor and Trametes villosa (2011)
  • Author(s): Y.Yanagi, N.Fujitake, 他
  • Journal Title: Humic Substances Research Volume: 8 Pages: 7-11
  • Peer Reviewed
• [Journal Article] Assessment of potentially reactive pools of aluminum in andisols using a five-step sequential extraction (2011)
  • Author(s): S.Dai, N.Fujitake, 他
  • Journal Title: Soil Science and Plant Nutrition Volume: 57 Issue: 4 Pages: 500-507
  • DOI: 10.1080/00380768.2011.598445
  • Peer Reviewed
• [Journal Article] Seasonal characteristics of surface water fulvic acids from Lake Biwa and Lake Tankai in Japan. (2011)
  • Author(s): N.Fujitake, 他
  • Journal Title: Limnology Volume: (印刷中)
  • NAID: 10030761028
  • Peer Reviewed
• [Journal Article] Characterization and grouping of aquatic fulvic acids isolated from clear-water rivers and lakes in Japan (2010)
  • Author(s): Tsuda, K., Mori, H., Asakawa, D., Yanagi, Y., Kodama, H., Nagao, S., Yonebayashi, K., Fujitake, N.
  • Journal Title: Water Research Volume: 44 Pages: 3837-3846
  • Peer Reviewed
• [Journal Article] Spatial distribution of<△14>^C values of organic matter in surface sediments off Saru River in northern Japan (2010)
  • Author(s): Nagao, S., Irino, T., Aramaki, T., Ikehara, K., Katayama, H., Otosaka, S., Uchida, M., Shibata Y.
  • Journal Title: one year after a flood event in 2006. Radiocarbon Volume: 52 Pages: 1068-1077
  • Peer Reviewed
• [Journal Article] Effects of rainfall on carbon isotopes of POC in the Teshio River, northern Japan (2010)
  • Author(s): Aramaki, T., Nagao, S., Nakamura, Y., Uchida, M., Shibata Y.
  • Journal Title: Radiocarbon Volume: 52 Pages: 808-814
  • Peer Reviewed
• [Journal Article] Carbon isotopes and lignin composition of POC in a small river in Bekanbeushi Moor, northern Japan (2010)
  • Author(s): Nagao, S., Aramaki, T., Seki, O., Uchida, M., Shibata Y.
  • Journal Title: Nucl. Inst. Method Phys. Res Volume: 268 Pages: 1098-1101
  • Peer Reviewed
• [Journal Article] Characterization of UV-absorbing organic matter in Lake Biwa using high-performance liquid chromatography/mass spectrometry (2010)
  • Author(s): Wada, C., Hayakawa, K., Suzuki, T., Kumagai, T., Sugiyama, Y.
  • Journal Title: Verh. Internat. Verein. Limnol Volume: 30 Pages: 1520-1524
  • Peer Reviewed
• [Journal Article] Characterization and grouping of aquatic fulvic acids isolated from clear-water rivers and lakes in Japan. (2010)
  • Author(s): K.Tsuda, N.Fujitake, 他
  • Journal Title: Water Research Volume: 44 Pages: 3837-3846
  • Peer Reviewed
• [Journal Article] Effects of ecological succession on surface mineral horizons in Japanese volcanic ash soil. (2010)
  • Author(s): Y.Iimura, N.Fujitake, 他
  • Journal Title: Geoderma Volume: 159 Pages: 122-130
  • Peer Reviewed
• [Journal Article] Chemical properties of aquatic fulvic acids isolated from Lake Biwa, a clear water system in Japan (2009)
  • Author(s): Fujitake, N., Kodama, H., Nagao, S., Tsuda, K., and Yonebayashi, K.
  • Journal Title: Humic Substances Research Volume: 5/6 Pages: 45-53
  • Peer Reviewed
• [Journal Article] Chemical properties of aquatic fulvic acids isolated from Lake Biwa, a clear water system in Japan (2009)
  • Author(s): 藤嶽暢英
  • Journal Title: Humic Substances Research 5/6 Pages: 45-53
  • Peer Reviewed
• [Presentation] C_<18>シリカゲルおよびDAX-8樹脂による溶存有機物の分別定量法の開発 (2012)
  • Author(s): 川端訓功, 飯村康夫, 大塚俊之, 廣田充, 藤嶽暢英
  • Organizer: 日本ペドロジー学会2012年度大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-06
• [Presentation] C_<18>シリカゲルおよびDAX-8樹脂による溶存有機物の分別定量法の開発 (2012)
  • Author(s): 川端訓功・藤嶽暢英, 他
  • Organizer: 日本ペドロジー学会
  • Place of Presentation: 首都大学東京
  • Year and Date: 2012-03-06
• [Presentation] 琵琶湖水中の水系フミン物質の季節および深度別の変化 (2012)
  • Author(s): 高田明弘, 津田久美子, 光嵜克敏, 白井秀門, 北野史子, 原都, 小島礼慈, 杉山裕子, 早川和秀, 藤嶽暢英
  • Organizer: 日本陸水学会近畿支部会第23回研究発表会
  • Place of Presentation: 奈良
  • Year and Date: 2012-03-03
• [Presentation] Quality analysis of dissolved organic matter by a sequential fractionation with C_<18> and DAX-8 resin (2011)
  • Author(s): Kawabata, K., Iimura, Y, Tsuda, K., Fujitake, N.
  • Organizer: Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Metla at Helsinki, Finland
  • Year and Date: 2011-09-20
• [Presentation] Characterization and grouping of humic substances in soils and waters by NMR spectra (2011)
  • Author(s): Fujitake, N.
  • Organizer: Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Metla at Helsinki, Finland
  • Year and Date: 2011-09-19
• [Presentation] Yenisei watershed (2011)
  • Author(s): Sugiyama, Y.
  • Organizer: Finnish-Japanese Seminar of the Dynamics of Dissolved organic Matter in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Metla at Helsinki, Finland
  • Year and Date: 2011-09-19
• [Presentation] Characterization of dissolved organic matter in soils and streams using ^1H-NMR spectra analysis (2011)
  • Author(s): Y.Saito, N.Fujitake, 他
  • Organizer: Finnish-Japanese Seminar of the Dynamics of DOM in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Finland, Helsinki
  • Year and Date: 2011-09-19
• [Presentation] Quality analysis of dissolved organic matter by a sequential fractionation with C18 and DAX-8 resin (2011)
  • Author(s): K.Kawabata, N.Fujitake, 他
  • Organizer: Finnish-Japanese Seminar of the Dynamics of DOM in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Finland, Helsinki
  • Year and Date: 2011-09-19
• [Presentation] Characterization and grouping of humic substances in soils and waters by NMR spectra (2011)
  • Author(s): N.Fujitake
  • Organizer: Finnish-Japanese Seminar of the Dynamics of DOM in Forested Catchments under Human and Environmental Impacts
  • Place of Presentation: Finland, Helsinki
  • Year and Date: 2011-09-19
• [Presentation] 黒ボク土における腐植の集積とメカニズム (2011)
  • Author(s): 藤嶽暢英
  • Organizer: 日本ペドロジー学会第50回公開シンポジウム
  • Place of Presentation: 帯広(招待講演)
  • Year and Date: 2011-09-02
• [Presentation] 高緯度地域における溶存有機物(DOM)の化学構造特性の把握 (2011)
  • Author(s): 齋藤友里・藤嶽暢英, 他
  • Organizer: 日本ペドロジー学会
  • Place of Presentation: 帯広
  • Year and Date: 2011-09-02
• [Presentation] C_<18>およびDAX-8による水抽出有機物疎水性画分の細分画法の検討 (2011)
  • Author(s): 川端訓功・藤嶽暢英, 他
  • Organizer: 日本ペドロジー学会
  • Place of Presentation: 帯広
  • Year and Date: 2011-09-02
• [Presentation] C_<18>およびDAX-8による水抽出有機物疎水性画分の細分画法の検討 (2011)
  • Author(s): 川端訓功, 飯村康夫, 津田久美子, 藤嶽暢英
  • Organizer: 日本ペドロジー学会2011年度大会
  • Place of Presentation: 帯広
  • Year and Date: 2011-09-01
• [Presentation] 高緯度地域における溶存有機物(DOM)の化学構造特性の把握 (2011)
  • Author(s): 齋藤友里, 川端訓功, Mike Starr, 藤嶽暢英
  • Organizer: 日本ペドロジー学会2011年度大会
  • Place of Presentation: 帯広
  • Year and Date: 2011-09-01
• [Presentation] 土壌中の溶存ラミン物質の分画法の開発 (2010)
  • Author(s): 川端訓功・藤嶽暢英, 他
  • Organizer: 日本腐植物質学会第26回講演会講演要旨集
  • Place of Presentation: 筑波大学(東京都)
  • Year and Date: 2010-11-29
• [Presentation] 溶存フミン物質の分画法の開発 (2010)
  • Author(s): 川端訓功・飯村康夫・津田久美子・藤嶽暢英
  • Organizer: 日本腐植物質学会第26回講演会
  • Place of Presentation: つくば
  • Year and Date: 2010-09-29
• [Presentation] MASEF:土壌腐植とは何だろうか?～土壌腐植は炭素動態にいかに関わるのか (2010)
  • Author(s): 川東正幸
  • Organizer: 日本生態学会会第57回全国大会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-03-15
• [Presentation] ポドゾルの溶脱層および集積層から抽出される可動性腐植物質の特徴 (2009)
  • Author(s): 小泉佳彦・渡邉哲弘・柳由貴子・藤嶽暢英・谷昌幸
  • Organizer: 日本土壌肥料学会2009年度大会
  • Place of Presentation: 京都
  • Year and Date: 2009-09-16
• [Presentation] 固相抽出を用いた琵琶湖溶存有機物の化学分画に関する基礎的研究1 (2009)
  • Author(s): 北野史子, 西本鷹耶, 和田千弦, 熊谷哲, 津田久美子, 白井秀門, 藤嶽暢英, 早川和秀, 島野淳, 丸尾雅啓, 杉山裕子
  • Organizer: 第74回日本陸水学会
  • Place of Presentation: 大分
  • Year and Date: 2009-09-15
• [Presentation] ポドゾルの溶脱層および集積層から抽出される可動性腐植物質の特徴 (2009)
  • Author(s): 谷昌幸
  • Organizer: 日本土壌肥料学会2009年度大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-09-15
• [Presentation] The effects of ecological succession on the characteristic of soil humic substances in a Japanese volcanic ash soil. Concentration and chemical properties of humic substances. (2009)
  • Author(s): 藤嶽暢英
  • Organizer: International Symposium on Soil Organic Matter Dynamics : Land Use, Management and Global Change
  • Place of Presentation: Cheyenne Mountain Conference Center, CO, USA
  • Year and Date: 2009-07-07
• [Book] 構造特性から見た土壌から河川へのDOC流入経路,「土壌-河川-海生態系における溶存有機炭素の動態と機能」 (2011)
  • Author(s): 浅川大地, 藤嶽暢英
  • Publisher: 博友社
• [Book] 土壌成分との相互作用から見たDOMの浸透,「溶存有機物の動態と機能.土壌.河川.海を結んで.」 (2011)
  • Author(s): 川東正幸
  • Publisher: 博友社
• [Book] 河川水のDOMの特徴と生元素の運搬機構,「溶存有機物の動態と機能.土壌.河川.海を結んで.」 (2011)
  • Author(s): 長尾誠也
  • Publisher: 博友社
• [Book] 「土壌-河川-海生態系における溶存有機炭素の動態と機能」第2章 構造特性から見た土壌から河川へのDOC流入経路 (2011)
  • Author(s): 浅川大地, 藤嶽暢英
  • Total Pages: 112
  • Publisher: 博友社
• [Book] 第2章 構造特性から見た土壌から河川へのDOC流入経路,「土壌-河川―海生態系における溶存有機炭素の動態と機能」 (2010)
  • Author(s): 浅川大地
  • Publisher: 博友社(印刷中)
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/ans-soil/