| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
|---|
| Research Abstract |
Proteins are often covalently trapped on DNA, generating DNA-protein cross-links(DPCs), when cells are exposed to DNA-damaging agents. However, not much is known about how cells mitigate the adverse effects of DPCs. In the present study, we have developed novel methods for the detection of DPCs. Genomic DPCs were primarily eliminated by spontaneous hydrolysis, and excision repair played no significant role in the removal of DPCs. It was suggested that DPCs initially activated the ATR DNA-damage response pathway, followed by the ATM pathway.
|
