| Project/Area Number |
21310044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ENDOH Daiji 酪農学園大学, 獣医学群, 教授 (40168828)
IWATA Hisato 愛媛大学, 学内共同利用施設, 教授 (10271652)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUKAWA Tatsuhiko 鹿児島大学, 医歯学総合研究科, 教授 (40219100)
HORAI Sawako 鳥取大学, 地域学部, 准教授 (60512689)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | オミックス / エコトキシコロジー / カワウ / ゼブラフィッシュ / ダイオキシン / ニワトリ |
|---|
| Research Abstract |
To study possible impacts of environmental pollutants on gene expression in a variety of organisms, we developed a large-scale differential display system with primer sets that are common in 7 vertebrate species with detailed molecular background, based on degenerate oligonucleotide-primed PCR(DOP-PCR). Inverse repeat motif of 8 mer was found in most transcripts for 7 vertebrates examined from fish to primates and more than ten thousand motifs were commonly recognized in transcripts of these 7 species. Among these, we chose 275 common motifs that cover roughly 30% of transcripts throughout these species, making seven vertebrates-common DOP-PCR primers(common DOP primers). This system enabled us to find some genes responsive to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in fetal livers of chicken and common cormorant.
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