Large-scale differential display system with vertebrate-common degenerate oligonucleotide primers
| Project/Area Number |
21310044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ENDOH Daiji 酪農学園大学, 獣医学群, 教授 (40168828)
IWATA Hisato 愛媛大学, 学内共同利用施設, 教授 (10271652)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUKAWA Tatsuhiko 鹿児島大学, 医歯学総合研究科, 教授 (40219100)
HORAI Sawako 鳥取大学, 地域学部, 准教授 (60512689)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | オミックス / エコトキシコロジー / カワウ / ゼブラフィッシュ / ダイオキシン / ニワトリ |
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| Research Abstract |
To study possible impacts of environmental pollutants on gene expression in a variety of organisms, we developed a large-scale differential display system with primer sets that are common in 7 vertebrate species with detailed molecular background, based on degenerate oligonucleotide-primed PCR(DOP-PCR). Inverse repeat motif of 8 mer was found in most transcripts for 7 vertebrates examined from fish to primates and more than ten thousand motifs were commonly recognized in transcripts of these 7 species. Among these, we chose 275 common motifs that cover roughly 30% of transcripts throughout these species, making seven vertebrates-common DOP-PCR primers(common DOP primers). This system enabled us to find some genes responsive to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in fetal livers of chicken and common cormorant.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article]2009
Author(s)
藤田正一, ら
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Journal Title
魚類のP450酵素系(P450の分子生物学 第2版)(講談社)
Pages: 195-208
Related Report
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[Journal Article] Large-scale survey of mitochondrial D-loop of the Red-crowned Crane Grus japonensis in Hokkaido, Japan by convenient genotyping method
Author(s)
Miura Y, Shiomi A, Shiraishi J, Makita K, Asakawa M, Kitazawa T, Hiraga T, Momose Y, Momose K, Masatomi H, Teraoka H.
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Journal Title
J Vet Med Sci
Volume: (in press)
NAID
Related Report
Peer Reviewed
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[Journal Article] Changes of Mercury Contamination in Red-Crowned Cranes, Grus japonensis, in East Hokkaido, Japan
Author(s)
Teraoka H, Tagami Y, Kudo M, Miura Y, Okamoto E, Matsumoto F, Koga K, Uebayashi A, Shimura R, Inoue M, Momose K, Masatomi H, Kitazawa T, Hiraga T, Subramanian A.
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Journal Title
Arch Environ Contam Toxicol
Volume: (in press)
Related Report
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[Presentation] 北海道におけるタンチョウの水銀汚染の原因についての検討2011
Author(s)
岡本絵梨佳, 久道萌, 北澤多喜雄, 寺岡宏樹, 水野直治, 古賀公也, 志村良治, 井上雅子, 百瀬邦和, 正富宏之, 中山翔太, 池中良徳, 石塚真由美
Organizer
日本鳥学会2011年度大会
Place of Presentation
大阪市立大学杉本キャンパス(大阪市)
Year and Date
2011-09-18
Related Report
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[Presentation] 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin responsive transcripts in avian embryonic liver, identified by large-scale differential display system with vertebrate-common degenerate oligonucleotide primers2011
Author(s)
Teraoka H, Ito S, Ukai N, Kim E-Y, Iwata H, Kitazawa T, Hiraga T and Endoh D.
Organizer
50th Society of Toxicology Annual Meeting
Place of Presentation
Washington Convention Center(Washington, D. C., USA)
Year and Date
2011-03-08
Related Report
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[Presentation] Role of CYP1C genes in the reduced mesencephalic vein blood flow caused by 2, 3, 7, 8-tetrachlorodibenzo-pdioxin in the zebrafish embryo2009
Author(s)
Kubota A, Harano R, Iwanaga T, Woodin BR, Stegeman JJ, Hiraga T and Teraoka H.
Organizer
27th New England Membrane Enzyme Group(NUTMEG) Conference
Place of Presentation
Woods Hole Oceanographic Institution(Woods Hole, MA, USA)
Year and Date
2009-10-05
Related Report
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