Study of membrane-linked signaling pathways for evaluation of estrogenic chemicals

• Project/Area Number: 21310046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KIYAMA Ryoiti 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00240739)
• Co-Investigator(Renkei-kenkyūsha): SAKUMA Yuko 日本医科大学, 医科研究科, 教授 (70094307) ; KIYAMA Yuko 日本医科大学, 医科研究科, 講師 (60234390)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: プロテオーム / エストロゲン / シグナル伝達 / シグナルカスケード / 脳神経系 / シグナル伝達経路 / 化学物質管理評価 / 環境物質 / 膜共役系 / 受容体 / 遺伝子機能 / 天然物

Research Abstract

In the present study, we found new signaling pathways for estrogenic chemicals and created a new way of evaluation of such chemicals by means of the profiling of the expression of estrogen-responsive genes and a comprehensive analysis of intracellular signal transduction.

Research Products

• [Journal Article] Estrogenic activity of bio-degradation products of C-heavy oil revealed by gene expression profiling using an oligo-DNA microarray system (2012)
  • Author(s): Zhu, Y., Kitamura, K., Maruyama, M., Higashihara, T., Kiyama, R.
  • Journal Title: Environ. Pollution Volume: 168 Pages: 10-14
  • DOI: 10.1016/j.envpol.2012.04.005
  • Peer Reviewed
• [Journal Article] Evaluation of cellular response to perfluorooctane sulfonate in human umbilical vein endothelial cells. (2012)
  • Author(s): Liao, Y., Wang, J., Huang, Q.-s., Fang, C., Kiyama, R., Shen, H. and Sijun Dong, S.
  • Journal Title: Environ. Pollution Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Estrogen-like activity and dual roles in cell signaling of an Agaricus blazei Murrill mycelia-dikaryon extract (2012)
  • Author(s): Dong, S., Furutani, Y., Suto, Y., Furutani, M., Zhu, Y., Yoneyama, M., Kato, T., Itabe, H., Nishikawa, T., Tomimatsu, H., Tanaka, T., Kasanuki, H., Masaki, T., Kiyama, R. and Matsuoka, M.
  • Journal Title: Microbiol. Res Volume: 167 Issue: 4 Pages: 231-237
  • DOI: 10.1016/j.micres.2011.09.003
  • Peer Reviewed
• [Journal Article] Evaluation of cellular response to perfluorooctane sulfonate in human umbilical vein endothelial cells (2012)
  • Author(s): Liao, Y., Wang, J., Huang, Q.-s, Fang, C., Kiyama, R., Shen, H., Sijun Dong, S.
  • Journal Title: Toxicol.In Vitro Volume: 26 Issue: 3 Pages: 421-428
  • DOI: 10.1016/j.tiv.2012.01.011
  • Peer Reviewed
• [Journal Article] Estrogenic activity of bio-degradation products of C-heavy oil revealed by gene expression profiling using an oligo-DNA microarray system (2012)
  • Author(s): Zhu, Y., Kitamura, K., Maruyama, M., Higashihara, T., Kiyama, R.
  • Journal Title: Environ.Pollution Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Effects of BIG1 and KANK1 on cell polarity and directed migration during wound healing (2011)
  • Author(s): Li, C.-C., Kuo, J.-C., Waterman, C. M., Kiyama, R., Moss, J. and Vaughan, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Issue: 48 Pages: 19228-19233
  • DOI: 10.1073/pnas.1117011108
  • Peer Reviewed
• [Journal Article] A conserved regulatory element in the mammalian beta-globin promoters (2011)
  • Author(s): Kiyama, R. and Wada-Kiyama, Y.
  • Journal Title: J. Mol. Evol Volume: 73 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Application of Fluolid-Orange-labeled probes for DNA microarray and immunological assays (2011)
  • Author(s): Zhu, Y., Ogaeri, O., Suzuki, J.-i., Dong, S., Aoyagi, T., Mizuki, K., Takasugi, M., Isobe, S.-i. and Kiyama, R.
  • Journal Title: Biotechnol. Letts Volume: 33 Issue: 9 Pages: 1759-1766
  • DOI: 10.1007/s10529-011-0646-0
  • Peer Reviewed
• [Journal Article] Functions of Kank1 and carcinogenesis (2011)
  • Author(s): Kakinuma, N., Zhu, Y., Ogaeri, T., Suzuki J.-i. and Kiyama, R.
  • Journal Title: Tumor Suppressors(Richard Schortemeyer III, ed.) Pages: 161-173
  • URL: http://www.novapublishers.com
  • Peer Reviewed
• [Journal Article] A induces a rapid activation of Erk1/2 through GPR30 in human breast cancer cells (2011)
  • Author(s): Dong, S., Terasaka, S. and Kiyama, R. Bisphenol
  • Journal Title: Environmental Pollution Volume: 159 Issue: 1 Pages: 212-218
  • DOI: 10.1016/j.envpol.2010.09.004
  • Peer Reviewed
• [Journal Article] A conserved regulatory element in the mammalian beta-globin promoters (2011)
  • Author(s): Kiyama, R., Wada-Kiyama, Y.
  • Journal Title: J.Mol.Evol. Volume: 73 Issue: 3-4 Pages: 101-108
  • DOI: 10.1007/s00239-011-9459-y
  • Peer Reviewed
• [Journal Article] Bisphenol A induces a rapid activation of Erk1/2 through GPR30 in human breast cancer cells. (2011)
  • Author(s): Dong, S., Terasaka, S., Kiyama, R.
  • Journal Title: Environmental Pollution Volume: 159 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Functions of Kank1 and carcinogenesis. (2011)
  • Author(s): Kakinuma, N., Zhu, Y., Ogaeri, T., Suzuki J.-i., Kiyama, R.
  • Journal Title: Tumor Suppressors(Richard Schortemeyer III, ed.) Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Kank proteins : Structure, functions and diseases (2009)
  • Author(s): Kakinuma, N., Zhu, Y., Wang, Y., Roy B. C. and Kiyama R.
  • Journal Title: Cellular and Molecular Life Sciences Volume: 66 Issue: 16 Pages: 2651-2659
  • DOI: 10.1007/s00018-009-0038-y
  • Peer Reviewed
• [Journal Article] A major mutation of KIF21A associated with congenital fibrosis of the extraocular muscles type 1(CFEOM1) enhances translocation of Kank1 to the membrane (2009)
  • Author(s): Kakinuma, N. and Kiyama, R.
  • Journal Title: Biochem. Biophys. Res. Comm Volume: 386 Issue: 4 Pages: 639-644
  • DOI: 10.1016/j.bbrc.2009.06.109
  • Peer Reviewed
• [Journal Article] Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes (2009)
  • Author(s): Parveen, M., Zhu Y. and Kiyama, R.
  • Journal Title: FEBS Letters Volume: 583 Issue: 14 Pages: 2377-2384
  • DOI: 10.1016/j.febslet.2009.06.035
  • Peer Reviewed
• [Journal Article] Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes. (2009)
  • Author(s): Parveen, M., Zhu Y., Kiyama, R.
  • Journal Title: FEBS Letters 583 Pages: 2377-2384
  • Peer Reviewed
• [Journal Article] A major mutation of KIF21A associated with congenital fibrosis of the extraocular muscles type 1(CFEOM1)enhances translocation of Kankl to the membrane. (2009)
  • Author(s): Kakinuma, N., Kiyama, R.
  • Journal Title: Biochem. Biophys. Res.Comm. 386 Pages: 639-644
  • Peer Reviewed
• [Journal Article] Kank proteins : Structure, functions and diseases. (2009)
  • Author(s): Kakinuma, N., Zhu, Y., Wang, Y., Roy B.C., Kiyama R.
  • Journal Title: Cellular and Molecular Life Sciences 66 Pages: 2651-2659
  • Peer Reviewed
• [Presentation] Actin dynamics in the sexually dimorphic nucleus of the rat preoptic area during the critical period (2012)
  • Author(s): Y. Wada-Kiyama, C. Suzuki, T. Hamada, R. Kiyama, Y. Sakuma
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館
  • Year and Date: 2012-03-31
• [Presentation] ジャトロファ精製油および搾りかすの生理活性評価 (2012)
  • Author(s): 朱耘、木山亮一
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 茨城県産総研共用講堂
  • Year and Date: 2012-01-31
• [Presentation] 新規蛍光物質Fluolidを用いた多重染色法によるヒト腎臓癌の病理診断法の開発 (2012)
  • Author(s): Dilibaier Wuxiuer、魚返拓利、朱耘、鈴木順一朗、青柳貞一郎、礒部信一郎、木山亮一
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 茨城県産総研共用講堂
  • Year and Date: 2012-01-31
• [Presentation] テーラーメイド医療用診断機器(DNAチップ): DNAチップ<開発ガイドライン>次世代医療機器 (2012)
  • Author(s): 木山亮一
  • Organizer: 開発ガイドライン・評価指標セミナー
  • Place of Presentation: 東京都日本教育会館
  • Year and Date: 2012-01-20
• [Presentation] テーラーメイド医療用診断機器(DNAチップ):DNAチップ<開発ガイドライン> (2012)
  • Author(s): 木山亮一
  • Organizer: 次世代医療機器開発ガイドライン・評価指標セミナー
  • Place of Presentation: 東京都日本教育会館
  • Year and Date: 2012-01-20
• [Presentation] Signaling of estrogen to actin dynamics via cofilin controls sexually dimorophic formation of the rat preoptic area (2011)
  • Author(s): Y. Wada-Kiyama, C. Suzuki, T. Hamada, R. Kiyama, Y. Sakuma
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] DNAマイクロアレイアッセイを用いた重油微生物分解産物のエストロゲン活性の評価 (2011)
  • Author(s): 朱耘、北村恵子、丸山明彦、東原孝規、木山亮一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 新規蛍光色素Fluolidを用いた多重染色法によるヒト腎臓癌の病理診断法の開発 (2011)
  • Author(s): Dilibaier Wuxiuer、魚返拓利、朱耘、鈴木順一朗、青柳貞一郎、礒部信一郎、木山亮一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] オミックス解析による天然・人工化合物のエストロゲン活性評価 (2011)
  • Author(s): 木山亮一
  • Organizer: 第29回内分泌代謝学サマーセミナー
  • Place of Presentation: 宮城県艮陵会館
  • Year and Date: 2011-07-07
• [Presentation] オリゴDNAマイクロアレイアッセイによる重油微生物分解産物のエストロゲン活性評価 (2011)
  • Author(s): 北村恵子、丸山明彦、東原孝規、朱耘、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 茨城県産総研共用講堂
  • Year and Date: 2011-02-01
• [Presentation] Kank1によるDaam1及びRhoを介する中心体複製と細胞質分裂の制御 (2011)
  • Author(s): 魚返拓利、鈴木順一朗、Roy BC、垣沼直人、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 茨城県産総研共用講堂
  • Year and Date: 2011-02-01
• [Presentation] Expression profiling of the estrogen-responsive genes in response to capsaicinoids using an oligo-DNA microarray assay system (2011)
  • Author(s): 朱耘、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: 茨城県産総研共用講堂
  • Year and Date: 2011-02-01
• [Presentation] Expression profiling of the estrogen responsive genes in response tocapsaicinoids using an oligo-DNA microarray assay system (2011)
  • Author(s): 朱耘、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2011-02-01
• [Presentation] Kank1によるDaam1及びRhoを介する中心体複製と細胞質分裂の制御 (2011)
  • Author(s): 魚返拓利、鈴木順一朗、Roy BC、垣沼直人、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2011-02-01
• [Presentation] オリゴDNAマイクロアレイアッセイによる重油微生物分解産物のエストロゲン活性評価 (2011)
  • Author(s): 北村恵子、丸山明彦、東原孝規、朱耘、木山亮一
  • Organizer: 第10回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2011-02-01
• [Presentation] Effects of BIG1 and KANK1 on Cell Polarity and Directed Migration during Wound Healing (2010)
  • Author(s): C. Li, J. Kuo, R. Kiyama, J. Moss, M. Vaughan
  • Organizer: 50th Annual Meeting of the American Society of Cell Biology
  • Place of Presentation: Philadelphia Convention Center,(米国ペンシルベニア州)
  • Year and Date: 2010-12-11
• [Presentation] Intracellular signalling stimulated by synthetic estrogenic chemicals and natural phytoestrogens (2010)
  • Author(s): yoiti Kiyama
  • Organizer: 2nd Xiamen International Forum on Urban and Environment(XIFUE)
  • Place of Presentation: 厦門市(中国)中国科学院都市環境研究所
  • Year and Date: 2010-12-11
• [Presentation] Intracellular signalling stimulated by synthetic estrogenic chemicals and natural phytoestrogens (2010)
  • Author(s): Ryoiti Kiyama
  • Organizer: 2nd Xiamen International Forum on Urban and Environment(XIFLTE)
  • Place of Presentation: 厦門,中国(招待講演)
  • Year and Date: 2010-12-11
• [Presentation] Effects of BIG1 and KANK1 on Cell Polarity and Directed Migration during Wound Healing (2010)
  • Author(s): C.Li, J.Kuo, R.Kiyama, J.Moss, M.Vaughan
  • Organizer: 50th Annual Meeting of the American Society of Cell Biology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2010-12-11
• [Presentation] Kank1によるDaam1及びRhoを介する細胞質分裂の制御 (2010)
  • Author(s): 鈴木順一朗, Badal. C Roy,垣沼直人,木山亮一
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Kank1によるDaam1及びRhoを介する細胞質分裂の制御 (2010)
  • Author(s): 鈴木順一朗, Badal.C Roy, 垣沼直人, 木山亮一
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Evaluation of Estrogenic Activity of Flavonoids by an Oligo-DNA Microarray Assay (2010)
  • Author(s): Yun Zhu, Ryoiti Kiyama
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Evaluation of Estrogenic Activity of Flavonoids by an Oligo-DNA Microarray Assay (2010)
  • Author(s): Yun Zhu, Ryoiti Kiyama
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Estradiol-induced signaling involved in the sexual differentiation of the rat preoptic area (2010)
  • Author(s): Chiaki Suzuki, Tomohiro Hamada, Ryoiti Kiyama, Yasuo Sakuma, Yuko Wada-Kiyama
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Estradiol-induced signaling involved in the sexual differentiation of the rat preoptic area (2010)
  • Author(s): Chiaki Suzuki, Tomohiro Hamada, Ryoiti Kiyama, Yasuo Sakuma, Yuko Wada-Kiyama
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Diverse Regulatory Actions of BIG1 and 2 Related to their ARF-activaing and KAP Functions (2010)
  • Author(s): Chun-Chun Li, Xiaoyan Shen, Ermanno Puxeddu, Jean-Cheng Kuo, Ryoiti Kiyama, Gustavo Pacheco-Rodriguez, Joel Moss, and Martha Vaughan
  • Organizer: FASEB meeting(Arf Family G Proteins)
  • Place of Presentation: Carefree ResortホテルCarefree(米国アリゾナ州)
  • Year and Date: 2010-08-15
• [Presentation] Diverse Regulatory Actions of BIG1 and 2 Related to their ARF-activating and AKAP Functions (2010)
  • Author(s): Chun-Chun Li, Xiaoyan Shen, Ermanno Puxeddu, Jean-Cheng Kuo, Ryoiti Kiyama, Gustavo Pacheco-Rodriguez, Joel Moss, Martha Vaughan
  • Organizer: FASEB meeting (Arf Family G Proteins)
  • Place of Presentation: Carefree, AZ
  • Year and Date: 2010-08-15
• [Presentation] 先天性外眼筋線維症1型におけるKIF21AとKank1の機能 (2010)
  • Author(s): 魚返拓利, 垣沼直人, 木山亮一
  • Organizer: 第9回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2010-02-04
• [Presentation] Evaluation of Estrogenic Activity of Flavonoids and Phthalate esters by an Oligo-DNA Microarray Assay System (2010)
  • Author(s): Yun Zhu, Shin-ichiro Isobe, Ryoiti Kiyama
  • Organizer: 第9回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2010-02-04
• [Presentation] Kank1によるWnt-Daam1シグナル経路を介するactin stress fiberの形成阻害 (2010)
  • Author(s): 鈴木順一朗, Badal C.Roy, 垣沼直人, 木山亮一
  • Organizer: 第9回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: つくば
  • Year and Date: 2010-02-04
• [Presentation] 化学物質評価のためのDNAチップ (2010)
  • Author(s): 朱耘, 木山亮一
  • Organizer: TXテクノロジー・ショーケースinつくば2010「つくば研究祭」
  • Place of Presentation: つくば
  • Year and Date: 2010-01-22
• [Presentation] Agaricus blazei Murrill mycelia-dikaryon has estrogen-like activity and prevents vascular damage in atherosclerosis (2009)
  • Author(s): Sijun Dong, 古谷喜幸, 数籐由美子, 古谷道子, 米山誠, 加藤太一, 板部洋之, 西川俊郎, 富松宏文, 田中健志, 笠貫宏, 真崎知生, 木山亮一, 松岡瑠美子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Kank-family proteins regulate RhoA-dependent formation of actin stress fibers and cell migration (2009)
  • Author(s): Yun Zhu, 垣沼直人, 王勇, バダルCロイ, 木山亮一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Estrogen-regulated genes involved in the sexual differentiation of the rat preoptic area (2009)
  • Author(s): 鈴木千晶, 濱田知宏、 垣沼直人, 木山亮一, 佐久間康夫, 木山裕子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Correlation between Kank1 and Kif21A mutations on congenital fibrosis of the extraocular muscle type 1(CFOEM1) (2009)
  • Author(s): 垣沼直人, 木山亮一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Kank1 inhibits Wnt/Fz-induced actin organization (2009)
  • Author(s): Badal C.Roy, 鈴木順一朗, 垣沼直人, 木山亮一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] DNA microarray analysis for evaluation of estrogenicity of natural and synthetic compounds (2009)
  • Author(s): 木山亮一
  • Organizer: 中国科学院都市環境研究所「環境及び健康研究学術報告会」環境研究所
  • Place of Presentation: 中国厦門
  • Year and Date: 2009-11-26
• [Presentation] DNA microarray analysis for evaluation of estrogenicity of natural and synthetic compounds (2009)
  • Author(s): 木山亮一
  • Organizer: 中国科学院都市環境研究所「環境及び健康研究学術報告会」
  • Place of Presentation: 厦門(中国)
  • Year and Date: 2009-11-26
• [Book] バイオ知財入門 (2010)
  • Author(s): 木山亮一, 他
  • Total Pages: 257
  • Publisher: 三和書籍