Statistical Analysis of Single Cell Mechanics by Atomic Force Microscopy

• Project/Area Number: 21310079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Hokkaido University
• Principal Investigator: OKAJIMA Takaharu 北海道大学, 情報科学研究科, 准教授 (70280998)
• Co-Investigator(Kenkyū-buntansha): KAWAHARA Koichi 北海道大学, 情報科学研究科, 特任教授 (20125397)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 原子間力顕微鏡 / 多数細胞 / 細胞レオロジー / ナノ計測 / 細胞周期 / 細胞分化 / 細胞診断 / 統計解析 / 細胞力学 / 多変量解析 / レオロジー / 細胞アレイ

Research Abstract

We investigated the number distribution of single cell rheology by atomic force microscopy (AFM) and discuss the statistical data in connection with a modern model of cell rheology such as power-law rheology. (1) The number distribution of single cell rheology was measured as a function of external frequency. (2) The rheological behavior of cells was measured as cells were synchronized using serum starvation. (3) We succeeded to measure the ensemble-and time-averaged cell rheology and found differences between them. (4) We investigated the number distribution of power-law rheology of cells transformed from normal cells to immortalized cells.

Research Products

• [Journal Article] Effect of Isoproterenol on Local Contractile Behaviors of Rat Cardiomyocytes Measured by Atomic Force Microscopy (2012)
  • Author(s): Y.Mizutani, K.Kawahara, T.Okajima
  • Journal Title: Current Pharmaceutical Biotechnology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Atomic Force Microscopy for the Examination of Single Cell Rheology (2012)
  • Author(s): T.Okajima
  • Journal Title: Current Pharmaceutical Biotechnology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy (2011)
  • Author(s): A. Okada, Y. Mizutani, A. Subagyo, H. Hosoi, M. Nakamura, K. Sueoka, K. Kawahara, and T. Okajima
  • Journal Title: Appl. Phys. Lett Volume: 99
  • NAID: 120003783352
  • Peer Reviewed
• [Journal Article] Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy (2011)
  • Author(s): A. Miyaoka, Y. Mizutani, M. Tsuchiya, K. Kawahara and T. Okajima
  • Journal Title: Jpn. J. Appl. Phys Volume: 50
  • NAID: 210000071145
  • Peer Reviewed
• [Journal Article] Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy (2011)
  • Author(s): A.Okada, 他6名, T.Okajima
  • Journal Title: Appl.Phys.Lett. Volume: 99 Issue: 26 Pages: 263703-263703
  • DOI: 10.1063/1.3672225
  • NAID: 120003783352
  • Peer Reviewed
• [Journal Article] Negative-feedback regulation of ATP release : ATP release from cardiomyocytes is strictly regulated during ischemia (2011)
  • Author(s): Kunugi, 他2名, Okajima, T., Kawahara, K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 416 Issue: 3-4 Pages: 409-415
  • DOI: 10.1016/j.bbrc.2011.11.068
  • NAID: 120003762601
  • Peer Reviewed
• [Journal Article] Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy (2011)
  • Author(s): A.Miyaoka, 他3名, T.Okajima
  • Journal Title: Jpn.J.Appl.Phys. Volume: 50 Issue: 8S3 Pages: 08LB16-08LB16
  • DOI: 10.1143/jjap.50.08lb16
  • NAID: 210000071145
  • Peer Reviewed
• [Journal Article] Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy (2010)
  • Author(s): S.Hiratsuka, 他5名, T.Okajima
  • Journal Title: MRS Proceedings Volume: 1261
  • Peer Reviewed
• [Journal Article] The number distribution of complex shear modulus of single cells measured by atomic force microscopy (2009)
  • Author(s): S. Hiratsuka, Y. Mizutani, M. Tsuchiya, K. Kawahara, H. Tokumoto, T. Okajima
  • Journal Title: Ultramicroscopy Volume: 109 Pages: 937-941
  • Peer Reviewed
• [Journal Article] Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal ProbeAtomic Force Microscopy Jpn (2009)
  • Author(s): S. Hiratsuka, Y. Mizutani, A. Toda, N. Fukushima, K. Kawahara, H. Tokumoto and T. Okajima
  • Journal Title: J. Appl. Phys Volume: 48
  • Peer Reviewed
• [Journal Article] Effect of Isoproterenol on Local Contractile Behaviors of Rat Cardiomyocytes Measured by Atomic Force Microscopy Current
  • Author(s): Y. Mizutani, K. Kawahara, T. Okajima
  • Journal Title: Pharmaceutical Biotechnology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Atomic Force Microscopy for the Examination of Single Cell Rheology (Review)
  • Author(s): T. Okajima
  • Journal Title: Current Pharmaceutical Biotechnology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy The
  • Author(s): S. Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto, K. Kawahara, T. Okajima
  • Journal Title: 2010 MRS Spring Meeting Symposium U proceedings
  • Peer Reviewed
• [Presentation] Universal rheological properties of cells in intermediate states observed in stress relaxation of cell sheets (2012)
  • Author(s): M.Tsuchiya, Y.Mizutani, K.Kawahara, T.Okajima
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: サンディエゴコンベンションセンター(米国)
  • Year and Date: 2012-02-27
• [Presentation] Power-law rheology of normal and cancer cells by AFM (2012)
  • Author(s): R.Takahashi, Y.Mizutani, K.Kawahara, T.Okajima
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: サンディエゴコンベンションセンター(米国)
  • Year and Date: 2012-02-27
• [Presentation] Power-law rheology of normal and cancer cells investigated by atomic force microscopy (2012)
  • Author(s): R. Takahashi, Y. Mizutani, K. Kawahara and T. Okajima
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Temporal Variation of Single Cell Rheology Investigated by Atomic Force Microscopy, (2011.12.19-21, Toyo) (2011)
  • Author(s): P.G.Cai, Y.Mizutani, K.Kawahara, T. Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 洞爺湖万世閣(北海道)
  • Year and Date: 2011-12-19
• [Presentation] Ion current mapping of living cells using ion conductance microscopy (2011)
  • Author(s): Y.Mizutani, T.Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 洞爺湖万世閣(北海道)
  • Year and Date: 2011-12-19
• [Presentation] Cycle Dependence of Single Cell Rheology Investigated by AFM (2011)
  • Author(s): Y.Usuki, Y.Mizutani, M.Tsuchiya, T.Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 洞爺湖万世閣(北海道)
  • Year and Date: 2011-12-19
• [Presentation] Power-law rheology of normal and cancer cells investigated by atomic force microscopy (2011)
  • Author(s): R.Takahashi, Y.Mizutani, K.Kawahara, T.Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 洞爺湖万世閣(北海道)
  • Year and Date: 2011-12-19
• [Presentation] Temporal Variation of Single Cell Rheology Investigated by Atomic Force Microscopy (2011)
  • Author(s): P.G. Cai, Y. Mizutani, K. Kawahara and T. Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Toyo
• [Presentation] Cell Cycle Dependence of Single Cell Rheology Investigated by AFM (2011)
  • Author(s): Y. Usuki, Y. Mizutani, M. Tsuchiya and T. Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Toyo
• [Presentation] Power-law rheology of normal and cancer cells investigated by atomic force microscopy (2011)
  • Author(s): R. Takahashi, Y. Mizutani, K. Kawahara, T. Okajima
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Toyo
• [Presentation] AFMによる細胞レオロジー統計量の解析法 (2011)
  • Author(s): 蔡萍根、水谷祐輔、土屋雅博、岡嶋孝治
  • Organizer: 第59回レオロジー討論会
  • Place of Presentation: 群馬
• [Presentation] AFMによる単一細胞レオロジーの個体差の精密解析 (2011)
  • Author(s): 蔡萍根、水谷祐輔、土屋雅博、河原剛一、岡嶋孝治
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形
• [Presentation] 原子間力顕微鏡を用いた水平方向の力学刺激による細胞内カルシウム応答測定 (2011)
  • Author(s): 佐藤淳平、水谷祐輔、河原剛一、岡嶋孝治
  • Organizer: 第49回生物物理学会年会
  • Place of Presentation: 兵庫
• [Presentation] 原子間力顕微鏡を用いた正常細胞とがん細胞の粘弾性測定 (2011)
  • Author(s): 高橋亮輔、水谷祐輔、蔡萍根、岡嶋孝治
  • Organizer: 第49回生物物理学会年会
  • Place of Presentation: 兵庫
• [Presentation] Spatial and Temporal Dependence of Single Cell Rheology Investigated by Atomic Force Microscopy (2011)
  • Author(s): P.G. Cai, Y. Mizutani, M. Tsuchiya, K. Kawahara , and T. Okajima
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore
• [Presentation] 原子間力顕微鏡を用いた物理刺激による細胞カルシウム応答測定 (2011)
  • Author(s): 佐藤淳平,水谷祐輔,河原剛一,岡嶋孝治
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川
• [Presentation] AFMによる不死化過程における細胞粘弾性の細胞数分布解析 (2011)
  • Author(s): 水谷祐輔,蔡萍根,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川
• [Presentation] AFMによる細胞内ネットワーク構造変化に対する細胞粘弾性の研究 (2011)
  • Author(s): 蔡萍根,水谷祐輔,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第58回応用物理学関係連合講演会
  • Place of Presentation: 神奈川
• [Presentation] Time and Space Dependent Studies in Cell Rheology by Atomic Force Microscopy (2010)
  • Author(s): P.G.Cai, 他4名, T.Okajima
  • Organizer: 18th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 静岡
  • Year and Date: 2010-12-09
• [Presentation] Rheology of Single Cells under Serum Starvation Measured by Atomic Force Microscopy (2010)
  • Author(s): A.Miyaoka, 他3名, T.Okajima
  • Organizer: 18th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: 静岡
  • Year and Date: 2010-12-09
• [Presentation] 細胞力学のゆらぎ解析:生細胞アレイの原子間力顕微鏡測定 (2010)
  • Author(s): 蔡萍根, 他2名, 岡嶋孝治
  • Organizer: 第7回バイオオプティクス研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-03
• [Presentation] 原子間力顕微鏡を用いた正常・老化・不死化細胞の力学統計解析 (2010)
  • Author(s): 水谷祐輔,蔡萍根,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第49回日本生体医工学会北海道支部大会
  • Place of Presentation: 札幌
  • Year and Date: 2010-10-09
• [Presentation] AFMによる多数細胞レオロジーの普遍性と細胞個性の評価 (2010)
  • Author(s): 蔡萍根, (他4名), 岡嶋孝治
  • Organizer: 2010年春季応用物理学関係連合講演会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-03-18
• [Presentation] AFMによる形質転換過程における線維芽細胞の複素弾性率の解析 (2010)
  • Author(s): 水谷祐輔, (他5名), 岡嶋孝治
  • Organizer: 2010年春季応用物理学関係連合講演会
  • Place of Presentation: 神奈川
  • Year and Date: 2010-03-17
• [Presentation] Time and Space Dependent Studies in Cell Rheology by Atomic Force Microscopy (2010)
  • Author(s): P.G. Cai, Y. Mizutani, A. Miyaoka, M. Tsuchiya, K. Kawahara, T. Okajima
  • Organizer: 18th International Colloquium on SPM
  • Place of Presentation: Atagawa
• [Presentation] Rheology of Single Cells under Serum Starvation Measured by Atomic Force Microscopy (2010)
  • Author(s): Miyaoka, Y. Mizutani, M. Tsuchiya, K. Kawahara and T. Okajima
  • Organizer: 18th International Colloquium on SPM
  • Place of Presentation: Atagawa
• [Presentation] AFMによる形質転換過程における線維芽細胞の複素弾性率の解析 (2010)
  • Author(s): 水谷祐輔,蔡萍根,宮岡敦史,土屋雅博,春菜ゆかり,河原剛一,岡嶋孝治
  • Organizer: 第57回応用物理学関係連合講演会
  • Place of Presentation: 平塚
• [Presentation] Rheology of transformed cells investigated by AFM (2010)
  • Author(s): Y. Mizutani, P. G. Cai, M. Tsuchiya, K. Kawahara and T.Okajima
  • Organizer: International Scanning Probe Microscopy Conference Sapporo '10
  • Place of Presentation: 札幌
• [Presentation] Rheology of transformed fibroblast cells investigated by atomic force microscopy (2010)
  • Author(s): Y. Mizutani, P. G. Cai, M. Tsuchiya, K. Kawahara, and T. Okajima
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台
• [Presentation] AFMによる正常細胞から不死化細胞のナノレオロジー測定 (2010)
  • Author(s): 水谷祐輔,蔡萍根,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第58回レオロジー討論会
  • Place of Presentation: 仙台
• [Presentation] Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy (2010)
  • Author(s): T. Okajima, S. Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto and K. Kawahara
  • Organizer: 2010 MRS Spring Meeting, San Francisco
• [Presentation] 細胞力学のゆらぎ解析:生細胞アレイの原子間力顕微鏡測定 (2010)
  • Author(s): 蔡萍根、水谷祐輔、土屋雅博、岡嶋孝治
  • Organizer: 第7回バイオオプティクス研究会・理研シンポジウム「蛍光相関分光と情報伝達(7)」合同シンポジウム
  • Place of Presentation: 東京
• [Presentation] 原子間力顕微鏡による心筋細胞の拍動挙動の計測 (2010)
  • Author(s): 水谷祐輔,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
• [Presentation] Mechanics of Single Cells Cultured on Microarray Measured by Atomic Force Microscopy (2009)
  • Author(s): Ping-Gen Cai, (他4名), T.Okajima
  • Organizer: The 17^<th> International Colloquim on Scanning Probe Microscopy
  • Place of Presentation: 静岡
  • Year and Date: 2009-12-10
• [Presentation] Statistical Analysis of Single Cell Mechanics Investigated by Atomic Force Microscopy (2009)
  • Author(s): T.Okajima
  • Organizer: The 4^<th> International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-08
• [Presentation] 原子間力顕微鏡による生細胞レオロジーの時間領域測 (2009)
  • Author(s): 平塚伸一郎、水谷祐輔、河原剛一、徳本洋志、岡嶋孝治
  • Organizer: 第56回応用物理学会講演会
• [Presentation] 原子間力顕微鏡による生細胞レオロジーの時間領域測定 (2009)
  • Author(s): 平塚伸一郎、水谷祐輔、河原剛一、徳本洋志、岡嶋孝治
  • Organizer: 第56回応用物理学会講演会
• [Presentation] Mechanics of Spontaneously Beating Cardiac Myocytes Investigated by AFM (2009)
  • Author(s): Y. Mizutani, D. Matsumaya, S. Hiratsuka, K. Kawahara, H. Tokumoto, T. Okajima
  • Organizer: The Biophysical Society 53nd Annual Meeting
• [Presentation] 細胞周期を同調させた生細胞のAFMレオロジー測定 (2009)
  • Author(s): 宮岡敦史,平塚伸一郎,水谷祐輔,土屋雅博,河原剛一,岡嶋孝治
  • Organizer: 第70回応用物理学会学術講演会
  • Place of Presentation: 第70回応用物理学会学術講演会,富山
• [Presentation] AFMによる多数細胞のレオロジー測定 (2009)
  • Author(s): 岡嶋孝治
  • Organizer: 平成21年度日本顕微鏡学会SPM研究部会
  • Place of Presentation: 新潟
• [Presentation] 細胞の配列化と単一細胞力学計測 (2009)
  • Author(s): 岡嶋孝治
  • Organizer: 第6回バイオオプティクス研究会
  • Place of Presentation: 札幌
• [Presentation] Machanics of cardiac cells under hypertrophy induced by oxidative stress measured with an atomic force microscope(原子間力顕微鏡を用いた酸化ストレスによる細胞肥大過程の力学特性評価) (2008)
  • Author(s): Y. Mizutani, M. Tsuchiya, K. Kawahara, T. Okajima
  • Organizer: 第47回日本生物物理学会年会,ppS192
  • Place of Presentation: 第47回日本生物物理学会年会,徳島市
• [Book] プローブ顕微鏡の使い方第2章試料の作り方2.5バイオ試料の扱い (2009)
  • Author(s): 岡嶋孝治、実践編
  • Publisher: 走査プローブ顕微鏡-正しい実験とデータ解析のために必要なこと-(実験物理科学シリーズ6)
• [Remarks]
  • URL: http://labs.ist.hokudai.ac.jp/cell/
• [Patent(Industrial Property Rights)] 単一細胞の力学特性の計測方法および計測装置 (2011)
  • Inventor(s): 岡嶋孝治、蔡萍根、水谷祐輔、土屋雅博
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 2011-184403
  • Filing Date: 2011-08-26
• [Patent(Industrial Property Rights)] 単一細胞の力学特性の計測方法および計測装置 (2011)
  • Inventor(s): 岡嶋孝治, 他3名
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 2011-184403
  • Filing Date: 2011-08-26