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Study on interactions of proteins/peptides disrupting cell membranes with lipid membranes using the single giant unilamellar vesicle method

Research Project

Project/Area Number 21310080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionShizuoka University

Principal Investigator

YAMAZAKI Masahito  静岡大学, 創造科学技術大学院, 教授 (70200665)

Co-Investigator(Kenkyū-buntansha) OKA Toshihiko  静岡大学, 理学部, 講師 (60344389)
TAMBA Yukihiro  鈴鹿高専, 教養教育科, 講師 (50436911)
Co-Investigator(Renkei-kenkyūsha) URISU Tsuneo  分子科学研究所, 生命錯体分子科学研究領域, 教授 (50249950)
NARUSE Keiji  岡山大学, 医歯薬学総合研究科, 教授 (40252233)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Keywords一分子科学 / 生体膜 / ポア形成 / 抗菌ペプチド / 蛋白質毒素 / 生物物理学 / ナノバイオ / 巨大リポソーム(GUV) / 単一GUV法 / 蛋白質毒素ライセニン / 抗菌ペプチドーマガイニン2 / 毒性蛋白質 / ライセニン
Research Abstract

We investigated the interactions of proteins/peptides disrupting cell membranes with lipid membranes using the single GUV method, where GUV is giant unilamellar vesicle with diameter greater than 10μm. We found that the rate constants of antimicrobial peptide magainin 2-induced pore formation in lipid membranes are mainly determined by the surface concentration of magainin 2 in the membranes. The measurement of the rate constants of membrane permeation of fluorescent probes through the magainin 2-induced pores revealed that the radius of the pore changes with time at the pore formation. We also revealed the pore formation induced by protein toxin lysenin, which can bind sphingomyelin specifically, and obtained membrane permeability coefficients of a fluorescent probe, calcein, through the pores in various lipid membranes

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (106 results)

All 2011 2010 2009 Other

All Journal Article (19 results) (of which Peer Reviewed: 15 results) Presentation (83 results) Remarks (4 results)

  • [Journal Article] A membrane filtering method for the purification of giant unilamellar vesicles2011

    • Author(s)
      Yukihiro Tamba, Hiroaki Terashima, and Masahito Yamazaki
    • Journal Title

      Chem. Phys. Lipids

      Volume: 164 Pages: 351-358

    • NAID

      120005573101

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kinetics of Low pH-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleo yl phosphatidy lserine/Monoolein2011

    • Author(s)
      Mahay Md. Alam, Toshihiko Oka, Noboru Ohta, and Masahito Yamazaki
    • Journal Title

      J. Chem. Phys.

      Volume: 134

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous insertion of lipopolysacc harideinto lipid membranes from aqueous solution2011

    • Author(s)
      Jahangir Md. Alam, and Masahito Yamazaki
    • Journal Title

      Chem. Phys. Lipids

      Volume: 164 Pages: 166-174

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 膜で測る:生体膜の機能やダイナミクスを解明する単一GUV法2011

    • Author(s)
      山崎昌一
    • Journal Title

      実験医学

      Volume: 29巻7 Pages: 48-54

    • Related Report
      2011 Annual Research Report 2011 Final Research Report 2010 Annual Research Report
  • [Journal Article] A membrane filtering method for the purification of giant unilamellar vesicles2011

    • Author(s)
      Y.Tamba
    • Journal Title

      Chem.Phys.Lipids

      Volume: 164 Issue: 5 Pages: 351-358

    • DOI

      10.1016/j.chemphyslip.2011.04.003

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetics of Low pH-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleoylphosphatidylserine/Monoolein2011

    • Author(s)
      M.M.Alam
    • Journal Title

      J.Chem.Phys.

      Volume: 134 Issue: 14

    • DOI

      10.1063/1.3575240

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous insertion of lipopolysaccharide into lipid membranes from aqueous solution2011

    • Author(s)
      J.M.Alam
    • Journal Title

      Chem.Phys.Lipids

      Volume: 164 Pages: 166-174

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic pathway of antimicrobial peptide magainin2-induced pore formation in lipid membranes2010

    • Author(s)
      Yukihiro Tamba, Hirotaka Ariyama, VictorLevadny, and Masahito Yamazaki
    • Journal Title

      J. Phys. Chem. B

      Volume: 114 Pages: 12018-12026

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 単一GUV法による抗菌ペプチドのポア形成の研究2010

    • Author(s)
      山崎昌一
    • Journal Title

      生物物理

      Volume: 50巻6 Pages: 296-297

    • NAID

      10026965330

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic pathway of antimicrobial peptide magainin 2-induced pore formation in li id membranes2010

    • Author(s)
      Y.Tamba
    • Journal Title

      J.Phys.Chem.B

      Volume: 114 Pages: 12018-12026

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 単一GUV法による抗菌ペプチドのボア形成の研究2010

    • Author(s)
      山崎昌一
    • Journal Title

      生物物理

      Volume: 50 Pages: 296-297

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Magainin 2-induced pore formation in membrane depends on its concentration in membrane interface2009

    • Author(s)
      Yukihiro Tamba, and Masahito Yamazaki
    • Journal Title

      J. Phys. Chem. B

      Volume: 113 Pages: 4846-4852

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Size of the Pore in Lipid Membranes Induced by Antimicrobial Peptide Magainin2009

    • Author(s)
      Hirotaka Ariyama, Yukihiro Tamba, Victor Levadny, and Masahito Yamazaki
    • Journal Title

      Micro-Nano Mechatronics and Human Science

      Volume: Nov. 8-11 Pages: 208-213

    • Related Report
      2011 Final Research Report
  • [Journal Article] 単一GUV法を用いたペプチドと脂質膜の相互作用の解析2009

    • Author(s)
      山崎昌一
    • Journal Title

      膜

      Volume: 34巻3 Pages: 126-132

    • NAID

      10026335954

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trans formation between Liposomes and Cubic Phases of Biological Lipid Membranes Induced by Modulation of Electrostatic Interactions2009

    • Author(s)
      Masahito Yamazaki
    • Journal Title

      Advances in Planar Lipid Bilayers and Liposomes

      Volume: 9 Pages: 163-209

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transformation between Liposomes and Cubic Phases of Biological Lipid Membranes Induced by Modulation of Electrostatic Interactions2009

    • Author(s)
      山崎昌一
    • Journal Title

      Advances in Planar Lipid Bilayers and Liposomes 9

      Pages: 163-209

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 単一GUV法を用いたペプチドと脂質膜の相互作用の解析2009

    • Author(s)
      山崎昌一
    • Journal Title

      膜 34

      Pages: 126-132

    • NAID

      10026335954

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Size of the Pore in Lipid Membranes Induced by Antimicrobial Peptide Magainin 22009

    • Author(s)
      H.Ariyama
    • Journal Title

      2009 IEEE International Symposium on Micro-Nano Mechatronics and Human Science

      Pages: 208-213

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Magainin 2-induced pore formation in membrane depends on its concentration in membrane interface2009

    • Author(s)
      Y.Tamba
    • Journal Title

      J.Phys.Chem.B 113

      Pages: 4846-4852

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The Single GUV Method for Probing the Antimicrobial Peptide-Induced Pore Formation in Lipid Membranes2011

    • Author(s)
      Masahito Yamazaki
    • Organizer
      1st Annual Symposium of Antimicrobial Research
    • Place of Presentation
      Beijing International Convention Center(Beijing, China)
    • Year and Date
      2011-12-01
    • Related Report
      2011 Final Research Report
  • [Presentation] The Single GUV Method for Probing the Antimicrobial Peptide-Induced Pore Formation in Lipid Membranes2011

    • Author(s)
      M.Yamazaki
    • Organizer
      1st Annual Symposium of Antimicrobial Research
    • Place of Presentation
      Beijing International Convention Center, Beijing, China(招待講演)
    • Year and Date
      2011-12-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Single GUV Method for Probing Dynamics and Functions of Biomembranes2011

    • Author(s)
      Masahito Yamazaki
    • Organizer
      International ERATO Symposium on Lipid Structures in and around Proteins
    • Place of Presentation
      Hotel Hankyu Expo Park(Suita)
    • Year and Date
      2011-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] The Single GUV Method for Probing Dynamics and Functions of Biomembrane2011

    • Author(s)
      M.Yamazaki
    • Organizer
      International ERATO Symposium on Lipid Structures in and around Proteins
    • Place of Presentation
      Hotel Hankyu Expo Park, Osaka(招待講演)
    • Year and Date
      2011-11-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mechanism of antimicrobial peptide magainin 2-induced pore formation in lipid membranes2011

    • Author(s)
      Tomoki Takahashi
    • Organizer
      17th International Biophysics Congress(IUPAB)
    • Place of Presentation
      China National Convention Center Beijing, China
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous Insertion of Lip polysaccharide into Lipid Membranes from Aqueous Solution2011

    • Author(s)
      Jahangir Md. ALAM
    • Organizer
      17th International Biophysics Congress(IUPAB)
    • Place of Presentation
      China National Convention Center Beijing, China
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanism of antimicrobial peptide magainin 2-induced pore formation in lipid membranes2011

    • Author(s)
      T.Takahashi
    • Organizer
      17^<th> International Biophysics Congress (IUPAB)
    • Place of Presentation
      China National Convention Center, Beijing, China
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] The single GUV method reveals the antimicrobial peptide-induced pore formation in lipid membranes2011

    • Author(s)
      M.Yamazaki
    • Organizer
      17^<th> International Biophysics Congress (IUPAB)
    • Place of Presentation
      China National Convention Center, Beijing, China
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Spontaneous Insertion of Lipopolysaccharide into Lipid Membranes from Aqueous Solution2011

    • Author(s)
      J.M.ALAM
    • Organizer
      17^<th> International Biophysics Congress (IUPAB)
    • Place of Presentation
      China National Convention Center, Beijing, China
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] The single GUV method reveals the antimicrobial peptide-induced pore for mation in lipid membranes2011

    • Author(s)
      Masahito Yamazaki
    • Organizer
      17th International Biophysics Congress(IUPAB)
    • Place of Presentation
      China National Convention Center Beijing, China
    • Year and Date
      2011-10-31
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetics of Lowp H-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleoyl phosphatidylserine/Monoolein2011

    • Author(s)
      Masahito Yamazaki
    • Organizer
      17th International Biophysics Congress(IUPAB)
    • Place of Presentation
      China National Convention Center Beijing, China
    • Year and Date
      2011-10-31
    • Related Report
      2011 Final Research Report
  • [Presentation] Protein Toxin Lysenin-Induced Membrane Permeability : the Single GUV Method Study2011

    • Author(s)
      Jahangir Md. ALAM
    • Organizer
      17th International Biophysics Congress(IUPAB)
    • Place of Presentation
      China National Convention Center Beijing, China
    • Year and Date
      2011-10-31
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetics of Low pH-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleoylphosphatidylserine/Monoolein2011

    • Author(s)
      M.Yamazaki
    • Organizer
      17^<th> International Biophysics Congress (IUPAB)
    • Place of Presentation
      China National Convention Center, Beijing, China
    • Year and Date
      2011-10-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Protein Toxin Lysenin-Induced Membrane Permeability : the Single GUV Method Study2011

    • Author(s)
      J.M.ALAM
    • Organizer
      17^<th> International Biophysics Congress (IUPAB)
    • Place of Presentation
      China National Convention Center, Beijing, China
    • Year and Date
      2011-10-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Single GUV Method Reveals the Peptide/Protein-Induced Pore Formation in Lipid Membranes2011

    • Author(s)
      Masahito Yamazaki
    • Organizer
      International Symposium on Synthesizing Life & Biological System
    • Place of Presentation
      Senri Life Science Center(Toyonaka)
    • Year and Date
      2011-10-25
    • Related Report
      2011 Final Research Report
  • [Presentation] The Single GUV Method Reveals the Peptide/Protein-Induced Pore Formation in Lipid Membranes2011

    • Author(s)
      M.Yamazaki
    • Organizer
      International Symposium on Synthesizing Life & Biological Systems
    • Place of Presentation
      Senri Life Science Center, Osaka(招待講演)
    • Year and Date
      2011-10-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of Binding of Magainin 2 to Lipid Membranes on Surface Area and Volume of single GUVs2011

    • Author(s)
      Tomoki Takahashi
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      姫路(兵庫県立大学・姫路書写キャンパス)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Dependence of Lysenin-Induced Membrane Permeability on Cholesterol and Lysenin Concentration in the Membrane Surface2011

    • Author(s)
      Jahangir Md. ALAM
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      姫路(兵庫県立大学・姫路書写キャンパス)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] A membrane filtering method for purification and concentration of giant unilamellar vesicles2011

    • Author(s)
      Yukihiro Tamba
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      姫路(兵庫県立大学・姫路書写キャンパス)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Effects of Binding of Magainin 2 to Lipid Membranes on Surface Area and Volume of single GUVs2011

    • Author(s)
      T.Takahashi
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      兵庫県立大学・姫路書写キャンパス(兵庫県)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dependence of Lysenin-Induced Membrane Permeability on Cholesterol and Lysenin Concentration in the Membrane Surface2011

    • Author(s)
      J.M.ALAM
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      兵庫県立大学・姫路書写キャンパス(兵庫県)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] A membrane filtering method for purification and concentration of giant unilamellar vesicles2011

    • Author(s)
      Y.Tamba
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      兵庫県立大学・姫路書写キャンパス(兵庫県)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Kinetics of Low pH-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleoyl phosphatidylserine/Monoolein2011

    • Author(s)
      M. Yamazaki
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      姫路(兵庫県立大学・姫路書写キャンパス)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetics of Low pH-Induced Lamellar to Bicontinuous Cubic Phase Transition in Dioleoylphosphatidylserine/Monoolein2011

    • Author(s)
      M.Yamazaki
    • Organizer
      日本生物物理学会第49回年次大会
    • Place of Presentation
      兵庫県立大学・姫路書写キャンパス(兵庫県)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 抗菌性ペプチドのマガイニン2が脂質膜面に誘起するポア形成のメカニズム2011

    • Author(s)
      高橋智貴
    • Organizer
      第1回ソフトマター研究会
    • Place of Presentation
      キャンパスプラザ京都、(京都府)
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蛋白質毒素ライセニンが誘起する脂質膜中のポア形成:単一GUV法による研究2011

    • Author(s)
      J.M.ALAM
    • Organizer
      第1回ソフトマター研究会
    • Place of Presentation
      キャンパスプラザ京都,(京都府)
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低いpHにより誘起される生体脂質膜の液晶相からキュービック相への相転移のキネティックス2011

    • Author(s)
      山崎昌一
    • Organizer
      第1回ソフトマター研究会
    • Place of Presentation
      キャンパスプラザ京都,(京都府)
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 単一GUV法による抗菌ペプチドのポア形成の研究2011

    • Author(s)
      山崎昌一
    • Organizer
      第29回物性物理化学研究会
    • Place of Presentation
      京都大学薬学部記念講堂
    • Year and Date
      2011-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 単一GUV法による抗菌ペプチドのポア形成の研究2011

    • Author(s)
      山崎昌一
    • Organizer
      第29回物性物理化学研究会「膜のダイナミズムと薬物透過:基礎から予測・制御で」
    • Place of Presentation
      京都大学薬学部記念講堂、京都府(招待講演)
    • Year and Date
      2011-05-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 外来物質との相互作用による生体膜のトポロジー変化のダイナミクスとメカニズム2011

    • Author(s)
      山崎昌一
    • Organizer
      特定領域研究「ソフトマター物理」第5回領域研究会
    • Place of Presentation
      東京(東京大学・弥生講堂)
    • Year and Date
      2011-01-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] The Single GUV Method Reveals the Antimicrobial Peptide-Induced Pore Formation in Lipid Membranes2010

    • Author(s)
      Masahito Yamazaki
    • Organizer
      1st Annual World Congress of NanoMedicine-2010
    • Place of Presentation
      Beijing International Convention Center(Beijing, China)
    • Year and Date
      2010-10-25
    • Related Report
      2011 Final Research Report
  • [Presentation] The Single GUV Method Reveals the Antimicrobial Peptide-Induced Pore Formation in Lipid Membranes2010

    • Author(s)
      M.Yamazaki
    • Organizer
      BIT's 1st Annual World Congress of Nano Medicine-2010
    • Place of Presentation
      Beijing International Convention Center, Beijing, China 招待講演
    • Year and Date
      2010-10-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of transportan-10on membrane2010

    • Author(s)
      Hirotaka Ariyama
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Membrane filtering method for purification of giant unilamellar vesicles2010

    • Author(s)
      Yukihiro Tamba
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Effect of transportan-10 on membrane permeability of single DOPC-GUVs2010

    • Author(s)
      H.Ariyama
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Construction of microarray of GUVs of oil-free membranes for single GUV method2010

    • Author(s)
      H.Terashima
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Protein toxin lysenin-inducedpore for mation in lipid membranes : the single GUV method study2010

    • Author(s)
      Rei Takagi
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Protein toxin lysenin-inducedpore formation in lipid membranes : the single GUV method study2010

    • Author(s)
      Rei Takagi
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanism of subcritical pore for mation in charged lipid membranes induced by antimicrobial peptide-magainin22010

    • Author(s)
      Victor Levadny
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of mechanical tension on the leakage of internal contents of liposomes of biomembranes2010

    • Author(s)
      Tomoki Takahashi
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Kinetic pathway of antimicrobial peptide magainin 2-inducedpore formation in lipid membranes2010

    • Author(s)
      Masahito Yamazaki
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Protein toxin lysenin-induced pore formation in lipid membranes : the single GUV method study2010

    • Author(s)
      R.Takagi
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mechanism of subcritical pore formation in charged lipid membranes induced by antimicrobial peptide-magainin 22010

    • Author(s)
      V.Levadny
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic pathway of antimicrobial peptide magainin 2-induced pore formation in lipid membranes2010

    • Author(s)
      M.Yamazaki
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Lipopolysaccharide induced shape changes and vesicle fission of giant unilamellar vesicles of lipid membranes2010

    • Author(s)
      Jahangir Md. Alam
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic of low-p Hinducedtrans formation of bilayer membrane to cubic phases in DOPS/MO membranes2010

    • Author(s)
      Mahay Md. Alam
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Lipopolysaccharide-induced shape changes and vesicle fission of giant unilamellar vesicles of lipid membranes2010

    • Author(s)
      J.M.Alam
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic of low-pH-induced transformation of bilayer membrane to cubic phases in DOPS/MO membranes2010

    • Author(s)
      M.M.Alam
    • Organizer
      日本生物物理学会第48回年次大会
    • Place of Presentation
      仙台(東北大学・川内北キャンパス)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic Pathway of Antimicrobial Peptide Magainin 2-InducedPore For mation in Lipid Membranes2010

    • Author(s)
      Masahito Yamazaki
    • Organizer
      ISSP(The Institute for Solid State Physics) International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ. Tokyo, Kashiwa
    • Year and Date
      2010-08-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic Pathway of Antimicrobial Peptide Magainin 2-Induced Pore Formation in Lipid Membranes2010

    • Author(s)
      M.Yamazaki
    • Organizer
      ISSP International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ.Tokyo, Kashiwa 招待講演
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] A subcritical half-poreformation in lipid vesicles induced by antimicrobial peptide2010

    • Author(s)
      Victor Levadny
    • Organizer
      ISSP International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ. of Tokyo, Kashiwa
    • Year and Date
      2010-08-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic of low-pH-induced transformation of bilayer membrane to bicontinuous cubic phases in dioleoyl phosphatidylserine/monoolein membranes2010

    • Author(s)
      Mahay Md. Alam
    • Organizer
      ISSP International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ. of Tokyo, Kashiwa
    • Year and Date
      2010-08-23
    • Related Report
      2011 Final Research Report
  • [Presentation] A subcritical half-pore formation in lipid vesicles induced by antimicrobial peptide2010

    • Author(s)
      V.Levadny
    • Organizer
      ISSP International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ.of Tokyo, Kashiwa
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic of low-pH-induced transformation of bilayer membrane to bicontinuous cubic phases in dioleoylphosphatidylserine/monoolein membranes2010

    • Author(s)
      M.M.Alam
    • Organizer
      ISSP International Workshop of Soft Matter Physics
    • Place of Presentation
      IPMU Lecture Hall at Univ.of Tokyo, Kashiwa
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic pathway of antimicrobial peptide magainin 2-inducedpore for mation in lipid membranes2010

    • Author(s)
      Masahito Yamazaki
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2010-08-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic of low-pH-induced transformation of bilayer membrane to bicontinuous cubic phases in dioleoyl phosphatidylserine/monoolein membranes2010

    • Author(s)
      Mahay Md. Alam
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2010-08-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanism of subcritical pore formation in charged lipid vesicles induced by antimicrobial peptide magainin22010

    • Author(s)
      Victor Levadny
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2010-08-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Interaction of lipopolysaccharide with lipid membranes2010

    • Author(s)
      Jahangir Md. Alam
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2010-08-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic of low-pH-induced transformation of bilayer membrane to bicontinuous cubic phases in dioleoylphosphatidylserine/monoolein membranes2010

    • Author(s)
      M.M.Alam
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara
    • Year and Date
      2010-08-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Kinetic pathway of antimicrobial peptide magainin 2-induced pore formation in lipid membranes2010

    • Author(s)
      M.Yamazaki
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara
    • Year and Date
      2010-08-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mechanism of subcritical pore formation in charged lipid vesicles induced by antimicrobial peptide magainin 22010

    • Author(s)
      V.Levadny
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara
    • Year and Date
      2010-08-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Interaction of lipopolysaccharide with lipid membranes2010

    • Author(s)
      J.M.Alam
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara
    • Year and Date
      2010-08-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Protein toxin lysenin-inducedpore for mation in lipid membranes : the single GUV method study2010

    • Author(s)
      Rei Takagi
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2010-08-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Protein toxin lysenin-induced pore formation in lipid membranes : the single GUV method study2010

    • Author(s)
      R.Takagi
    • Organizer
      International Symposium on Non-Equilibrium Soft Matter 2010
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara
    • Year and Date
      2010-08-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 抗菌ペプチドーマガイニン2の脂質膜中でのボア形成のキネティックスパスウエイ2010

    • Author(s)
      山崎昌一
    • Organizer
      ナノ学会第8回大会
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎)
    • Year and Date
      2010-05-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体膜のトポロジー変化のダイナミクスとメカニズム2010

    • Author(s)
      山崎昌一
    • Organizer
      特定研究「ソフトマター物理」A02班・班会議
    • Place of Presentation
      お茶の水女子大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 抗菌ペプチド・マガイニン2が膜中に誘起するポア形成のキネティックスパスウエイ2010

    • Author(s)
      山崎昌一
    • Organizer
      特定領域「バイオ操作」第8回公開シンポジウム
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2010-03-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単一GUV法を用いた生体膜の機能・ダイナミクスの解析2010

    • Author(s)
      山崎昌一
    • Organizer
      大阪大学情報科学研究科
    • Place of Presentation
      四方研究室セミナー
    • Year and Date
      2010-02-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 単一GUV法を用いた生体膜の機能・ダイナミクスの解析2010

    • Author(s)
      山崎昌一
    • Organizer
      大阪大学 情報科学研究科専攻 ERATO四方研究室セミナー
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-02-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 単一GUV法を用いた生体膜の機能・ダイナミクスの解析2009

    • Author(s)
      山崎昌一
    • Organizer
      名古屋大学応用物理学教室談話会
    • Place of Presentation
      名古屋大学応用物理学教室
    • Year and Date
      2009-12-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 単一GUV法を用いた生体膜の機能・ダイナミクスの解析2009

    • Author(s)
      山崎昌一
    • Organizer
      名古屋大学 応用物理学教室 談話会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-12-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 抗菌性ペプチドによる脂質膜中のポア形成の素過程とそのメカニズム : 単一GUV法による研究2009

    • Author(s)
      山崎昌一
    • Organizer
      特定研究「ソフトマター物理」第3回公開シンポジウム
    • Place of Presentation
      京都大学・船井哲良記念講堂
    • Year and Date
      2009-11-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] The size of the pore in lipid membranes induced by antimicrobial peptide magainin 22009

    • Author(s)
      Masahito Yamazaki
    • Organizer
      2009 IEEE International Symposium on Micro-Nano Mechatronics and Human Science(MHS2009)
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2009-11-10
    • Related Report
      2011 Final Research Report
  • [Presentation] The size of the pore in lipid membranes induced by antimicrobial peptide magainin 22009

    • Author(s)
      山崎昌一
    • Organizer
      2009IEEE International Symposium on Micro-Nano Mechatronics and Human Science (MHS2009)
    • Place of Presentation
      Nagoya
    • Year and Date
      2009-11-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Kinetic pathway for the antimicrobial peptide magainin 2-inducedpore formation in lipid membranes2009

    • Author(s)
      Masahito Yamazaki
    • Organizer
      3rd International Symposium on Nanomedicine(ISNM2009-2)
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      2009-11-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Kinetic pathway for the antimicrobial peptide magainin 2-induced pore formation in lipid membranes2009

    • Author(s)
      山崎昌一
    • Organizer
      3^<rd> International Symposium on Nanomedicine (ISNM2009-2)
    • Place of Presentation
      Okazaki
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] The mechanism of the epigallocatechin gallate-induced burst of giant unilamellar vesicles2009

    • Author(s)
      Y.Tamba
    • Organizer
      日本生物物理学会第47回年次大会
    • Place of Presentation
      徳島 (アスティ徳島)
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Lysenin-Induced Pore Formation in Lipid Membranes in Liquid-Ordered Phase : the Single GUV Method Study2009

    • Author(s)
      山崎昌一
    • Organizer
      日本生物物理学会第47回年次大会
    • Place of Presentation
      徳島 (アスティ徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] The Size of the Pore in Lipid Membranes Induced by Antimicrobial Peptide Magainin 22009

    • Author(s)
      H.Ariyama
    • Organizer
      日本生物物理学会第47回年次大会
    • Place of Presentation
      徳島 (アスティ徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Mechanism of Subcritical Pore Formation in Charged Lipid Vesicles Induced by Antimicrobial Peptides2009

    • Author(s)
      V.Levadny
    • Organizer
      日本生物物理学会第47回年次大会
    • Place of Presentation
      徳島 (アスティ徳島)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Antimicrobial peptide magainin 2-Induced Pore For mation in Lipid Membranes : the Single GUV Method Study2009

    • Author(s)
      Masahito Yamazaki
    • Organizer
      International Symposium on Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes(INSMP)
    • Place of Presentation
      Shirankaikan at Kyoto University, Kyoto
    • Year and Date
      2009-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Antimicrobial peptide magainin 2-Induced Pore Formation in Lipid Membranes : the Single GUV Method Study2009

    • Author(s)
      山崎昌一
    • Organizer
      International Symposium on Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes (INSMP)
    • Place of Presentation
      Shirankaikan at Kyoto University, Kyoto
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 抗菌ペプチド : マガイニン2が脂質膜中に形成するポアの大きさ2009

    • Author(s)
      山崎昌一
    • Organizer
      特定領域「ソフトマター物理」・第4回領域研究会
    • Place of Presentation
      北海道大学・学術交流会館
    • Year and Date
      2009-07-01
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~spmyama/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~spmyama/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~spmyama/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ipc.shizuoka.ac.jp/~spmyama/index.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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