| Project/Area Number |
21310080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKA Toshihiko 静岡大学, 理学部, 講師 (60344389)
TAMBA Yukihiro 鈴鹿高専, 教養教育科, 講師 (50436911)
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| Co-Investigator(Renkei-kenkyūsha) |
URISU Tsuneo 分子科学研究所, 生命錯体分子科学研究領域, 教授 (50249950)
NARUSE Keiji 岡山大学, 医歯薬学総合研究科, 教授 (40252233)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
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| Keywords | 一分子科学 / 生体膜 / ポア形成 / 抗菌ペプチド / 蛋白質毒素 / 生物物理学 / ナノバイオ / 巨大リポソーム(GUV) / 単一GUV法 / 蛋白質毒素ライセニン / 抗菌ペプチドーマガイニン2 / 毒性蛋白質 / ライセニン |
|---|
| Research Abstract |
We investigated the interactions of proteins/peptides disrupting cell membranes with lipid membranes using the single GUV method, where GUV is giant unilamellar vesicle with diameter greater than 10μm. We found that the rate constants of antimicrobial peptide magainin 2-induced pore formation in lipid membranes are mainly determined by the surface concentration of magainin 2 in the membranes. The measurement of the rate constants of membrane permeation of fluorescent probes through the magainin 2-induced pores revealed that the radius of the pore changes with time at the pore formation. We also revealed the pore formation induced by protein toxin lysenin, which can bind sphingomyelin specifically, and obtained membrane permeability coefficients of a fluorescent probe, calcein, through the pores in various lipid membranes
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