| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Research Abstract |
Hyperthermophilic archaea are capable of adapting to changes in environmental conditions in spite of their small set of genes. This study focused on regulation of gene expression during environmental adaptation in the hyperthermophilic archaeon Thermococcus kodakarensis. We identified the NikR-dependent regulon probably functional in Ni^<2+>-transport, and Phr-dependent regulon for heat-shock responses. We also found that a pair of PIN domain protein and hypothetical protein, involved in repression of chitin-degradation genes, acted as a global factor for expression regulation of various gene in the overall genome, and the proteins interacted to each other to form a complex. Moreover, disruption of probable protein kinase gene(s) in the hyperthermophile resulted in changes of intracellular amount of many proteins, suggesting the presence of regulation systems through protein phosphorylation.
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