| Project/Area Number |
21310125
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ASAKAWA Shuichi 東京大学, 大学院・農学生命科学研究科, 教授 (30231872)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMORI Kazuhiro (独)産業技術総合研究所, 研究員 (60273025)
SHIMIZU Atsushi 慶應義塾大学, 医学部, 助教 (30327655)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Kosuke 慶應義塾大学, 医学部, 助教 (70245463)
MITSUYAMA Susumu 慶應義塾大学, 医学部, 助教 (30296727)
KOJIMA SABINE Kazuko 慶應義塾大学, 医学部, 研究員 (10445439)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 動物ゲノム / メダカ / トラフグ / 次世代シーケンサー / 小分子RNA / マイクロRNA / piRNA / トランスクリプトーム / miRNA / 遺伝子発現 / 魚類 / 発現フロフィール / 新規RNA |
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| Research Abstract |
In this study, we analyzed the sequences of small RNAs(miRNA, piRNA, siRNA) extracted form tissues(Fast muscle, slow muscle, intestine, eye, brain, liver, ovary, testis, and embryos) of medaka(Oryzias latipes) and torafugu(Takifugu rubripes), and showed the expression profiles of the RNAs. In the tissues except for ovary and testis, the major species of expression were miRNAs, whereas in the ovary and testis, those were assumed to be piRNAs. There were found many unknown RNA species that were commonly expressed in medaka and torafugu
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