| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Research Abstract |
Next generation sequencing offers lower cost and higher throughput than Sanger sequencing for human personal genome. To understand all genetic variants within personal genome, we carried out re-sequencing of two chromosomes(HSA21 and HSAY) separated from a human cell line and a sample from the HapMap JPT(Japanese) sets using the GAIIx sequencers. In a Japanese male genome, we identified over 3 million sequence variants(SNVs) at an average of 30-fold coverage. In addition, not only SNVs but also structural variants, such as indels and copy number variants, were detected.
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