| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Research Abstract |
We profiled recombinant human kinases by using in vitro kinase assay combined with quantitative phosphoproteomics approaches. As a result, 175, 574 kinase-substrate relationships were obtained from the in vitro assay using HeLa cell lysate as the substrate source. Based on the kinase-substrate combination data, we successfully extracted a total of 1, 525 phosphorylation motifs targeted by 303 kinases and classified the kinases to generate a novel phylogenic' kinome' tree based on in vitro substrates instead of the traditional tree based on the sequence homology of kinase domain. Finally, two approaches based on in vitro substrates and phosphorylation motifs were developed to predict kinases which are activated or inactivated by particular stimuli.
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