Interactome analysis of protein kinases
| Project/Area Number |
21310129
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied genomics
|
|---|
| Research Institution | Kyoto University (2010-2011)
Keio University (2009) |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
SUGIYAMA Naoyuki 慶應義塾大学, 政策メディア研究科, 特任講師 (50545704)
YOSHIHARA Hiroki 慶應義塾大学, 政策メディア研究科, 助教 (90348706)
MASUDA Takeshi 慶應義塾大学, 政策メディア研究科, 研究員 (70383940)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
|---|
| Keywords | 翻訳後修飾 / リン酸化プロテオーム / キナーゼ / リン酸化プロテオミクス / インタラクトーム |
|---|
| Research Abstract |
We profiled recombinant human kinases by using in vitro kinase assay combined with quantitative phosphoproteomics approaches. As a result, 175, 574 kinase-substrate relationships were obtained from the in vitro assay using HeLa cell lysate as the substrate source. Based on the kinase-substrate combination data, we successfully extracted a total of 1, 525 phosphorylation motifs targeted by 303 kinases and classified the kinases to generate a novel phylogenic' kinome' tree based on in vitro substrates instead of the traditional tree based on the sequence homology of kinase domain. Finally, two approaches based on in vitro substrates and phosphorylation motifs were developed to predict kinases which are activated or inactivated by particular stimuli.
|
Report
(4 results)
Research Products
(195 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Detection of Rap1A as a yessotoxin binding protein from blood cell membranes2010
Author(s)
S. Ujihara, T. Oishi, R. Mouri, R. Tamate, K. Konoki, N. Matsumori, M. Murata, Y. Oshima, N. Sugiyama, M. Tomita, and Y. Ishihama
-
Journal Title
Bioorg Med Chem Lett
Volume: 20
Issue: 22
Pages: 6443-6
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
[Journal Article] Detection of Rap1A as a yessotoxin binding protein from blood cell membranes2010
Author(s)
Satoru Ujihara, Tohru Oishi, Ryota Mouri, Rie Tamate, Keiichi Konoki, Nobuaki Matsumori, Michio Murata, Yasukatsu Oshima, Naoyuki Sugiyama, Masaru Tomita, Yasushi Ishihama
-
Journal Title
Bioorganic & Medicinal Chemistry Letters
Volume: 20
Pages: 6443-6446
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article]2009
Author(s)
杉山直幸
-
Journal Title
実験医学「プロテオミクスが解き明かす情報伝達ネットワーク」(羊土社)
Pages: 2552-2557
Related Report
-
[Journal Article]2009
Author(s)
京野完
-
Journal Title
細胞工学別冊「明日を拓く新次元プロテオミクス」(学研メディカル秀潤社)
Pages: 41-50
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] タンパク質の抽出と分画2011
Author(s)
増田豪; 杉山直幸; 石濱泰
Organizer
日本プロテオーム学会2011年会
Place of Presentation
朱鷺メッセ(潟県)
Year and Date
2011-07-28
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
