| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Research Abstract |
Although fluorescence-based visualization has been primarily applied to investigate the behavior of target protein in a cell, there are few methods for fluorescent labeling at the interacting site. Here, a simple and unique construction of a fluorophore at the biding domain of target protein has been achieved by sequential photoreactions using a non-fluorescent ligand molecule. The labeled protein can be used for the fluorescence-based substrate binding analysis
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