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Molecular dissection of prenyltransferase family involved in flavonoid functionalization and the enzyme engineering

Research Project

Project/Area Number 21310141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKyoto University

Principal Investigator

YAZAKI Kazufumi  京都大学, 生存圏研究所, 教授 (00191099)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsフラボノイド / プレニル化 / 酵素工学 / 膜蛋白質 / プラスチド局在 / プレニル化酵素 / ポリフェノール / 二次代謝 / 生合成 / プラスチド / プレニル基転移酵素 / イソプレノイド / 代謝工学 / クララ / ホップ / 有用物質生産
Research Abstract

The first isoflavone-specific prenyltransferase SfG6DT and the first chalcone-specific one SfiLDT have been cloned from cultured cells of Sophora flavescens by utilizing flavone-specific prenyltransferase SfN8DT-1. Both have 9 transmembrane alpha-helices and plastid-targeting signal, and by phylogenetic analysis it was turned out that all those flavonoid prenyltransferase belong to prenyltransferase family for vitamin E biosynthesis. Moreover, a new enzyme GmG4DT involved in phytoalexin biosynthesis in soybean has been identified. Beyond Leguminosae, flavonioid prenyltransferases have been isolated in Macaranga tanarius(Euphorbiaceae), as well. By making serial chimeric enzyme between SfN8DT and SfG6DT, a determinant region for substrate recognition could be determined. Furthermore, metabolic engineering using those enzyme genes have been carried out in plant and bacterial hosts.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2012 2011 2010 2009 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (16 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] HlPT-1,a membrane-bound prenyltransferase responsible for the biosynthesis of bitter acids in hops2012

    • Author(s)
      Tsurumaru Y, Sasaki K, Miyawaki T, Uto Y, Momma T, Umemoto N, Momose M, Yazaki K.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 417 Issue: 1 Pages: 393-398

    • DOI

      10.1016/j.bbrc.2011.11.125

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of coumarin-specific prenyltransferase activities in Citrus limon peel2012

    • Author(s)
      Munakata, R., et al
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic engineering for the production ofprenylated polyphenols in transgenic legume plants using bacterial and plantprenyltransferases2011

    • Author(s)
      Sugiyama, A., (11人略), Yazaki, K.
    • Journal Title

      Metab. Eng.

      Volume: 13 Issue: 6 Pages: 629-637

    • DOI

      10.1016/j.ymben.2011.07.003

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of amembrane-bound prenyltransferase specific for isoflavone from Sophora flavescens2011

    • Author(s)
      Sasaki, K., Tsurumaru, Y., Yamamoto, H., Yazaki, K.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Issue: 27 Pages: 24125-24134

    • DOI

      10.1074/jbc.m111.244426

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Tolerance gene for prenylated flavonoidencodes 26S proteasome regulatory subunit in Sophora flavescens2011

    • Author(s)
      Shitan, N., Yazaki, K.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 982-984

    • NAID

      10028272910

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of prenylated flavonoids in tomato fruits expressing a prenyltransferasegene from Streptomyces coelicolor A3(2)2011

    • Author(s)
      Koeduka, T., Yazaki, K.
    • Journal Title

      Plant Biol.

      Volume: 13 Issue: 2 Pages: 411-145

    • DOI

      10.1111/j.1438-8677.2010.00409.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Tolerance Gene for Prenylated Flavonoid Encodes a 26S Proteasome Regulatory Subunit in Sophora flavescens2011

    • Author(s)
      Shitan, N, et al
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 5 Pages: 982-984

    • DOI

      10.1271/bbb.100665

    • NAID

      10028272910

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)2011

    • Author(s)
      Koeduka, T., et al.
    • Journal Title

      Plant Biol.

      Volume: 13 Pages: 411-415

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two solanesyl diphosphate synthases with different subcellular localizations and the irrespective physiological roles in Oryzasativa2010

    • Author(s)
      Ohara, K., Sasaki, K., Yazaki, K.
    • Journal Title

      J. Exp. Bot.

      Volume: 61 Issue: 10 Pages: 2683-2692

    • DOI

      10.1093/jxb/erq103

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonenesynthase cDNA2010

    • Author(s)
      Ohara, K., Yazaki, K.
    • Journal Title

      Plant Biotech. J.

      Volume: 8 Issue: 1 Pages: 28-37

    • DOI

      10.1111/j.1467-7652.2009.00461.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An aromatic prenyltransferase-like gene HlPT-1 preferentially expressed in lupulin glands of hop2010

    • Author(s)
      Tsurumaru, Y., Yazaki, K.
    • Journal Title

      Plant Biotechnology

      Volume: 27 Issue: 2 Pages: 199-204

    • DOI

      10.5511/plantbiotechnology.27.199

    • NAID

      10026527307

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two solanesyl diphosphate synthases with different subcellular localizations and their respective physiological roles in Oryza sativa.2010

    • Author(s)
      Ohara, K., et al.
    • Journal Title

      J.Exp.Bot.

      Volume: 61 Pages: 2683-2692

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An aromatic prenyltransferase-like gene HlPT-1 preferentially expressed in lupulin glands of hop.2010

    • Author(s)
      Tsurumaru, Y., et al.
    • Journal Title

      Plant Biotech.

      Volume: 27 Pages: 199-204

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An aromatic prenyltransferase-like gene H1PT-1 preferentially expressed in lupulin glands of hop2010

    • Author(s)
      Tsurumaru, Y., et al.
    • Journal Title

      Plant Biotech. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of a cDNA for pterocarpan4-dimethylallyltransferase catalyzing the keyprenylation step in the biosynthesis ofglyceollin, a soybean phytoalexin2009

    • Author(s)
      Akashi, T., Sasaki, K., Aoki, T., Ayabe, S., Yazaki, K.
    • Journal Title

      PlantPhysiol.

      Volume: 149 Issue: 2 Pages: 683-693

    • DOI

      10.1104/pp.108.123679

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prenylation of aromatic compounds, a keydiversification of plant secondary metabolites2009

    • Author(s)
      Yazaki, K., Sasaki, K., Tsurumaru, Y.
    • Journal Title

      Phytochemistry

      Volume: 70 Issue: 15-16 Pages: 1739-1745

    • DOI

      10.1016/j.phytochem.2009.08.023

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prenylation of aromatic compounds, a key diversification of plant secondary metabolites2009

    • Author(s)
      Yazaki, K., et al.
    • Journal Title

      Phytochemistry 70

      Pages: 1739-1745

    • NAID

      120001712069

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional characterization of LePGT1, a membrane-bound prenyltransferase involved in the geranylation of parahydroxybenzoic acid2009

    • Author(s)
      Ohara, K., et al.
    • Journal Title

      Biochem.J. 421

      Pages: 231-241

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Flavonoid-specificprenyl transferases, a membrane-bound enzyme family responsible for polyphenol diversity2011

    • Author(s)
      Yazaki, K.
    • Organizer
      50th Phytochemical Society of Noth America
    • Place of Presentation
      KohalaCoast(Hawaii)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の芳香族化合物の高機能化を司るプレニル化酵素2011

    • Author(s)
      矢崎一史
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-23
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の芳香族化合物の高機能化を司るプレニル化酵素2011

    • Author(s)
      矢崎一史
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)(招待講演)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] プレニルトランスフェラーゼ及びカルコンイソメラーゼを果実特異的に発現する形質転換トマトの解析2011

    • Author(s)
      川崎崇
    • Organizer
      第29回日本植物細胞分子生物学会(福岡)大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] レモン由来のクマリンプレニル基転移酵素のクローングと機能解析2011

    • Author(s)
      井上剛史, 他
    • Organizer
      日本農芸化学会京都大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] プレニル基転移酵素の過剰発現によるトマト形質転換体のフラボノイドの解析2011

    • Author(s)
      川崎崇, 他
    • Organizer
      日本農芸化学会京都大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 機能性低分子としてのプレニル化ポリフェノールと生合成工学2010

    • Author(s)
      矢崎一史
    • Organizer
      第62回日本生物工学会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2011 Final Research Report
  • [Presentation] ホップ由来芳香族プレニル化酵素候補遺伝子HlPT-1の単離と組織別発現解析2010

    • Author(s)
      鶴丸優介, 他
    • Organizer
      植物細胞分子生物学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 熱帯樹オオバギのプレニル化フラボノイドの生合成遺伝子の研究2010

    • Author(s)
      前田容子, 他
    • Organizer
      植物細胞分子生物学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物の芳香族基質プレニル基転移酵素と機能性植物低分子2010

    • Author(s)
      矢崎一史
    • Organizer
      2010年度日本農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-31
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物の芳香族基質プレニル基転移酵素と機能性植物低分子2010

    • Author(s)
      矢崎一史
    • Organizer
      2010年度 日本農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Prenyltransferases for flavonoidsin plants and the utilization of the genes to engineer tomato plant2010

    • Author(s)
      Yazaki, K.
    • Organizer
      Crop Functional Genomics
    • Place of Presentation
      Jeju Island(Korea)
    • Related Report
      2011 Final Research Report
  • [Presentation] 生合成と蓄積に関する植物細胞の役割分担2009

    • Author(s)
      矢崎一史
    • Organizer
      第46回植物化学シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-12-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Prenyltransferase involved in the coupling of polyphenol and isoprenoid unit in plants2009

    • Author(s)
      矢崎一史
    • Organizer
      Terpnet 2009
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of the flavonoid-specific prenyltransferase SfN8DT-3 from Sophora flavesecens2009

    • Author(s)
      Yusuke Tsurumaru
    • Organizer
      Terpnet 2009
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-05-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Prenylation of aromatic compounds, a key diversification of plant secondary metabolites2009

    • Author(s)
      Yazaki, K.
    • Organizer
      Colloquium of the Priority Programme. Evolution of Metabolic Diversity.(SPP1152)
    • Place of Presentation
      Freising, Germany
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LPGE/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LPGE/

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規のプレニル化酵素活性を有するタンパク質とそれをコードする遺伝子2009

    • Inventor(s)
      矢崎一史、梅基直行
    • Industrial Property Rights Holder
      キリンホールディングス株式会社
    • Industrial Property Number
      2009-146754
    • Filing Date
      2009-06-19
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 新規のプレニル化酵素活性を有するタンパク質とそれをコードする遺伝子2009

    • Inventor(s)
      矢崎一史, ら
    • Industrial Property Rights Holder
      キリンホールディングス (株)
    • Industrial Property Number
      2009-146754
    • Filing Date
      2009-06-19
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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