| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Research Abstract |
The first isoflavone-specific prenyltransferase SfG6DT and the first chalcone-specific one SfiLDT have been cloned from cultured cells of Sophora flavescens by utilizing flavone-specific prenyltransferase SfN8DT-1. Both have 9 transmembrane alpha-helices and plastid-targeting signal, and by phylogenetic analysis it was turned out that all those flavonoid prenyltransferase belong to prenyltransferase family for vitamin E biosynthesis. Moreover, a new enzyme GmG4DT involved in phytoalexin biosynthesis in soybean has been identified. Beyond Leguminosae, flavonioid prenyltransferases have been isolated in Macaranga tanarius(Euphorbiaceae), as well. By making serial chimeric enzyme between SfN8DT and SfG6DT, a determinant region for substrate recognition could be determined. Furthermore, metabolic engineering using those enzyme genes have been carried out in plant and bacterial hosts.
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