| Project/Area Number |
21310144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kagoshima University |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
HASHIGUCHI Syuhei 鹿児島大学, 理工学研究科, 助教 (40295275)
伊東 祐二 鹿児島大学, 理工学研究科(工学系), 准教授 (60223195)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | アルツハイマー認知症 / M13ファージ / ワクチン / 自然免疫 / ミミックペプチド / アミロイドベーター / MyD88 / TLR9 / ヒト抗体 / Aβ42 / 受動免疫 / ファージライブラリ / J20 / オリゴマー |
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| Research Abstract |
Bacteriophage evokes strong antibody production by means of innate immunity. Recently, we have established B6 mimotope peptide(B6-C15) which mimics the structure of amyloid・42(A・) fibril using human single-chain Fv(B6) specific to A・42 fibril. This mimotope peptide binds to A・42 oligomers and inhibits formation of A・42 fibril in vitro. We investigated whether M13 bacteriophage displaying B6-mimotope peptide(B6-C15-phage) could produce A・42 fibril specific antibody response in mice. When mice were immunized i. p. with B6-C15-phage in PBS solution, they produced anti-A・42 fibril specific IgG response in two weeks. This finding suggested a promising vaccination strategy for immunotherapy of Alzheimer's disease without the involvement of A・42-specific T cell immunity(BBRC 402 : 19-22, 2010, and J. Neuroimmunol. 236 : 27-38, 2011).
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