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Development of a Novel Conductivity Detection Technique using Microbeads Coated with Gold Nanoparticles

Research Project

Project/Area Number 21350044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

NAGAOKA Tsutomu  大阪府立大学, 大学院・工学研究科, 教授 (00172510)

Co-Investigator(Renkei-kenkyūsha) SHIIGI Hiroshi  大阪府立大学, 工学研究科, 准教授 (70335769)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords化学センサ / 金ナノ粒子 / マイクロビーズ / 樹脂マイクロビーズ / ナノ粒子被覆ビーズ / DNA / 磁性樹脂ビーズ / 酵素 / 電気的検出 / ナノ粒子被覆マイクロビーズ / 電流検出 / 金メッキビーズ / 磁性樹脂マイクロビーズ / 導電性ポリマー / 過酸化
Research Abstract

In this research project, we have developed a novel detection system using plastic microbeads, which was coated with gold nanoparticles. The gold nanoparticle coated beads had an electrical conductivity ranging from 30 to a few mega ohm, and ligand coating was made on the gold layer by the self-assembly process. We have confirmed that the formation of a ligand-analyte linkage led to an increase in the electrical resistance of the microbeads.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (39 results)

All 2012 2011 2010 2009

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (21 results) Book (8 results)

  • [Journal Article] Synthesis and bioanalytical applications of specific-shaped metallic nanostructures : A review2012

    • Author(s)
      S. Tokonami, Y. Yamamoto, H. Shiigi, T. Nagaoka
    • Journal Title

      Anal. Chim. Acta

      Volume: 716 Pages: 76-91

    • DOI

      10.1016/j.aca.2011.12.025

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrical and thermal properties of conducting microbead prepared by green electroless plating method using gold nanoparticles2011

    • Author(s)
      S. Tokonami, S. Shirai, I. Ota, N. Shibutani, Y. Yamamoto, H. Shiigi, T. Nagaoka
    • Journal Title

      J. Electrochem. Soc.

      Volume: 158 Issue: 12 Pages: D689-D689

    • DOI

      10.1149/2.022112jes

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organic-inorganic Hybrid Nanoraspberry Consisted of Gold Nanoparticle and Aniline Oligomer2011

    • Author(s)
      R. Morita, R. Inoue, S. Tokonami, Y. Yamamoto, M. Nakayama, H. Nakao, H. Shiigi, T. Nagaoka
    • Journal Title

      J. Electrochem. Soc.

      Volume: 158 Issue: 4 Pages: K95-K95

    • DOI

      10.1149/1.3549166

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organic-inorganic Hybrid Nanoraspberry Consisted of Gold Nanoparticle and Aniline Oligomer2011

    • Author(s)
      R.Morita, R.Inoue, S.Tokonami, Y.Yamamoto, M.Nakayama, H.Nakao, H.Shiigi, T.Nagaoka
    • Journal Title

      J.Electrochem.Soc.

      Volume: 158

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microand nanosized molecularly imprinted polymers for high-throughput analytical applications2009

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      Anal. Chim. Acta

      Volume: 641 Issue: 1-2 Pages: 7-13

    • DOI

      10.1016/j.aca.2009.03.035

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activity enhancement of a screen-printed carbon electrode by modification with a gold nanoparticle for glucose determination2009

    • Author(s)
      M. Iwamoto, S. Tokonami, H. Shiigi, T. Nagaoka
    • Journal Title

      Research on Chemical Intermediates

      Volume: 35 Issue: 8-9 Pages: 913-930

    • DOI

      10.1007/s11164-009-0074-0

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Green Electroless Plating Method Using Gold Nanoparticles for Conducting Microbeads : Application to Anisotropic Conductive Films2009

    • Author(s)
      S. Tokonami, Y. Yamamoto, Y. Mizutani, I. Ota, H. Shiigi, T. Nagaoka
    • Journal Title

      J. Electrochem. Soc.

      Volume: 156 Issue: 12 Pages: D558-D558

    • DOI

      10.1149/1.3240594

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Green Electroless Plating Method Using Gold Nanoparticles for Conducting Microbeads : Application to Anisotropic Conductive Films2009

    • Author(s)
      S.Tokonami, Y.Yamamoto, Y.Mizutani, I.Ota, H.Shiigi, T.Nagaoka
    • Journal Title

      J.Electrochem.Soc. 156

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activity enhancement of a screen-printed carbon electrode by modification with a gold nanoparticle for glucose determination2009

    • Author(s)
      M.Iwamoto, S.Tokonami, H.Shiigi, T.Nagaoka
    • Journal Title

      Research on Chemical Intermediates 35

      Pages: 919-930

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Micro- and nanosized molecularly imprinted polymers for high-throughput analytical applications2009

    • Author(s)
      S.Tokonami, H.Shiigi, T.Nagaoka
    • Journal Title

      Anal.Chim.Acta 641

      Pages: 7-13

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Plastic Microbeads Coated with Au Nanoparticles for Analytical Applications2011

    • Author(s)
      T. Nagaoka, H. Shiigi, S. Tokonami, Y. Yamamoto
    • Organizer
      ICAS2011
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-05-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 金被覆バイオビーズの作製2010

    • Author(s)
      宮下尚之, 水谷祐太, 山本陽二郎, 床波志保, 椎木弘, 長岡勉
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      松江
    • Year and Date
      2010-05-16
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Plastic microbeads coated with Au nanoparticles for analytical applications2010

    • Author(s)
      T. Miyashita, Y. Mizutani, S. Tokonami, Y. Yamamoto, H. Shiigi, and T. Nagaoka
    • Organizer
      16th ICFIA
    • Place of Presentation
      Pattaya, Thailand
    • Year and Date
      2010-04-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 過酸化ポリピロールを用いるペプチドセンサの開発2010

    • Author(s)
      床波志保, 溝畑裕巳, 椎木弘, 長岡勉
    • Organizer
      電気化学会第77回大会
    • Place of Presentation
      富山大学
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 金ナノ粒子アレイ膜を利用したラベルフリーな電気的DNA検出2009

    • Author(s)
      床波志保, 溝畑裕己, 椎木弘, 長岡勉
    • Organizer
      第19回日本MRS学術シンポジウム
    • Place of Presentation
      横浜市開港記念会館
    • Year and Date
      2009-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] 金ナノ粒子アレイ膜を利用したラベルフリーな電気的DNA 検出2009

    • Author(s)
      床波志保, 溝畑裕己, 椎木弘, 長岡勉
    • Organizer
      第19回日本MRS学術シンポジウム
    • Place of Presentation
      横浜市開港記念会館
    • Year and Date
      2009-12-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNAフローアナリシスのためのナノ粒子電気抵抗検出器の開発2009

    • Author(s)
      床波志保, 溝畑裕己, 椎木弘, 長岡勉
    • Organizer
      第48回フローインジェクション分析講演会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2009-11-27
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 分子鋳型過酸化ポリピロール膜を用いたQCM検出器の開発2009

    • Author(s)
      溝畑裕己, 床波志保, 椎木弘, 長岡勉
    • Organizer
      第48回フローインジェクション分析講演会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2009-11-27
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 金ナノ粒子アレイを用いる電流検出法の開発2009

    • Author(s)
      長岡勉
    • Organizer
      同志社大学第29回分野別研究講演会
    • Place of Presentation
      同志社大学
    • Year and Date
      2009-10-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] センサー開発と流れ分析2009

    • Author(s)
      長岡勉
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学高等教育機能開発総合センター
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] HPLCを用いた皮膚成分測定2009

    • Author(s)
      太田至, 床波志保, 椎木弘, 長岡勉
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学高等教育機能開発総合センター
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 有機-無機ハイブリッドナノラズベリーの作製と特性評価2009

    • Author(s)
      森田亮輔, 床波志保, 山本陽二郎, 椎木弘, 長岡勉
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学高等教育機能開発総合センター
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 金ナノ粒子架橋構造を利用した電気抵抗型DNAセンサの開発2009

    • Author(s)
      床波志保, 椎木弘, 長岡勉
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学高等教育機能開発総合センター
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Performance Assessment of Molecularly Imprinted Polymer Based Sensors by Using Flow Injection Techniques2009

    • Author(s)
      S. Takeda, S. Tokonami, H. Shiigi, T. Nagaoka
    • Organizer
      FLOW ANALYSIS XI
    • Place of Presentation
      Mallorca
    • Year and Date
      2009-09-14
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Development of Electrical DNA Detection System by Using Open Bridge-Structured Gold Nanoparticle Array2009

    • Author(s)
      S. Tokonami, I. Ota, S. Shirai, H. Shiigi, T. Nagaoka
    • Organizer
      FLOW ANALYSIS XI
    • Place of Presentation
      Mallorca
    • Year and Date
      2009-09-14
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Open Bridge-Structured Gold Nanoparticle Film for Electrical DNA Detection2009

    • Author(s)
      S. Tokonami, I. Ota, S. Shirai, H. Shiigi, T. Nagaoka
    • Organizer
      EURO ANALYSIS 2009
    • Place of Presentation
      Convention center, Innsbruck
    • Year and Date
      2009-09-09
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Overoxidized Polypyrrole for Sensor Application2009

    • Author(s)
      Shiho Tokonami, Hiroshi Shiigi, Tsutomu Nagaoka
    • Organizer
      EURO ANALYSIS 2009
    • Place of Presentation
      Convention center, Innsbruck
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低環境負荷ナノめっき法による導電性ビーズの作製とその特性評価2009

    • Author(s)
      水谷佑太, 太田至, 床波志保, 山本陽二郎, 椎木弘, 長岡勉
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学
    • Year and Date
      2009-05-16
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 金ナノ粒子の分析化学的応用2009

    • Author(s)
      床波志保, 椎木弘, 長岡勉
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学
    • Year and Date
      2009-05-16
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 低環境負荷ナノめっき法により作製した導電性ビーズの表面および電気特性評価2009

    • Author(s)
      床波志保, 水谷佑太, 太田至, 山本陽二郎, 椎木弘, 長岡勉
    • Organizer
      ナノ学会第7回大会
    • Place of Presentation
      東京大学
    • Year and Date
      2009-05-09
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 過酸化ポリピロールを用いるペプチドセンサの開発2009

    • Author(s)
      床波志保, 溝畑裕己, 椎木弘, 長岡勉
    • Organizer
      電気化学会第77回大会
    • Place of Presentation
      富山大学
    • Year and Date
      2009-03-29
    • Related Report
      2009 Annual Research Report
  • [Book] Molecularly Imprinted Sensors : Overview and Applications2012

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka, Elsevier B. V.
    • Publisher
      (印刷中)
    • Related Report
      2011 Final Research Report
  • [Book] Molecularly Imprinted Sensors : Overview and Applications2012

    • Author(s)
      S.Tokonami, H.Shiigi, T.Nagaoka
    • Publisher
      Elsevier B.V.(印刷中)
    • Related Report
      2011 Annual Research Report
  • [Book] An Environmentally friendly Plastic Coating Technique Using Gold Nanoparticles for Nanoelectronic Device Applications in Environmentally Harmonious Chemistry for the 21th Century2010

    • Author(s)
      T. Nagaoka and H. Shiigi
    • Publisher
      Nova Science Publishers, Inc.
    • Related Report
      2011 Final Research Report
  • [Book] Gold-polymer Nanocomposites for Bioimaging and Biosensing in Nanocomposites2010

    • Author(s)
      N. Uehara and T. Nagaoka
    • Publisher
      WILEY-VCH
    • Related Report
      2011 Final Research Report
  • [Book] An Environmentally-friendly Plastic Coating Technique Using Gold Nanopart icles for Nanoelectronic Device Applications in Environmentally Harmonious Chemistry for the 21thCentury, Chapter 92010

    • Author(s)
      T.Nagaoka, H.Shiigi
    • Total Pages
      220
    • Publisher
      Nova Science Publishers, In
    • Related Report
      2010 Annual Research Report
  • [Book] Gold-polymer Nanocomposites for Bioimaging and Biosensing in Nanocomposites, Chapter 62010

    • Author(s)
      N.Uehara, T.Nagaoka
    • Total Pages
      445
    • Publisher
      WILEY-VCH
    • Related Report
      2010 Annual Research Report
  • [Book] Nanostructured Thin Films and Surfaces, Volume 5, Chapter 52009

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Publisher
      WILEY-VCH
    • Related Report
      2011 Final Research Report
  • [Book] Nanostructured Thin Films and Surfaces, Volume 5, Chapter 52009

    • Author(s)
      S.Tokonami, H.Shiigi, T.Nagaoka
    • Total Pages
      431
    • Publisher
      WILEY-VCH
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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