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Advanced methods for molecular sensing in brains and its application to nanobio sensing

Research Project

Project/Area Number 21350045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionNihon University

Principal Investigator

SUGAWARA Masao  日本大学, 文理学部, 教授 (50002176)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Keywords生体分析 / 急性脳スライス / 神経伝達物質 / L-グルタミン酸 / マイクロセンサー / 可視イメージング / グルタミン酸 / 海馬スライス / 酵素アッセイ / MMP-9 / 脂質二分子膜 / メソポーラスシリカ / サブスタンスP / 脳内物質 / 海馬 / グルタミン酸センサー / リポソームアレイ / 成長ホルモン / イムノアッセイ / バイオセンサー / 成長ホルモン関連ペプチド / 蛍光分析
Research Abstract

New methods with high sensitivity, such as a capillary-based enzyme sensor, array-type immune sensing, lipid bilayer sensors, and an imaging method with high spatiotemporal resolution have been developed for brain substances, especially neurotransmitters, and some of them were applied to acute brain slices for elucidating neuronal signal transduction mechanisms.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2012 2011 2010 2009

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (20 results) Book (2 results)

  • [Journal Article] Real time monitoring of matrix metalloproteinase-9 collagenolytic activity with a surface plasmon resonance biosensor2011

    • Author(s)
      A. Shoji, M. Kabeya, M. Sugawara
    • Journal Title

      Anal. Biochem

      Volume: 419 Issue: 1 Pages: 53-60

    • DOI

      10.1016/j.ab.2011.07.031

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In situ modification of lipid-loaded MCM-41 channels with bovine serum albumin at a planar lipid bilayer for biosensing2011

    • Author(s)
      Keiichiro Nozawa
    • Journal Title

      Sensors and Actuators B : Chemical

      Volume: 160 Issue: 1 Pages: 139-144

    • DOI

      10.1016/j.snb.2011.07.024

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous monitoring of extracellular L-glutamate level and excitatory postsynaptic potential in region CA1 of mouse hippocampal slices2011

    • Author(s)
      S. Hozumi, K. Ikezawa, A. Shoji, A. Hirano-Iwata, T. Bliss, M. Sugawara
    • Journal Title

      Biosensors & Bioelectronics

      Volume: 26 Pages: 2975-2980

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Monitoring of Extracellular L-Glutamate Level and Excitatory Postsynaptic Potential in Region CA1 of Mouse Hippocampal Slices2011

    • Author(s)
      S.Hozumi
    • Journal Title

      Biosensors&Bioelectronics

      Volume: 26 Pages: 2975-2980

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In Vitro Measurements of Extracellular L-Glutamate Level in Region CA3 of Mouse Hippocampal Slices under Chemical Stimulation2010

    • Author(s)
      H. Chiba, Y. Deguchi, E. Kanazawa, J. Kawai, K. Nozawa, Atsushi Shoji, M. Sugawara
    • Journal Title

      Analytical Sciences

      Volume: 26 Issue: 10 Pages: 1103-1106

    • DOI

      10.2116/analsci.26.1103

    • NAID

      10026785917

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A reusable liposome array and its application to assay of growth hormone related peptides2010

    • Author(s)
      A. Shoji, E. Sugimoto, S. Orita, K. Nozawa, A. Yanagida, Y. Shibusawa, M. Sugawara
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 397 Pages: 1377-1381

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trypsin-immobilized mesoporous silica MCM-41 as a sensing system for ions and molecules2010

    • Author(s)
      K. Nozawa, A. Shoji and M. Sugawara
    • Journal Title

      Suparamolecular chemistry

      Volume: 22 Issue: 7-8 Pages: 389-395

    • DOI

      10.1080/10610278.2010.483733

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Vitro Measurements and Comparison of Extracellular L-Glutamate Level in Region CA3 of Mouse Hippocampal Slices under Chemical Stimulation2010

    • Author(s)
      H.Chiba
    • Journal Title

      Anal.Sci

      Volume: 26 Pages: 1103-1106

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Reusable Liposome Array and Its application to Assay of Growth Hormone Related Peptides2010

    • Author(s)
      A.Shoji
    • Journal Title

      Anal.Bioanal.Chem.

      Volume: 397 Pages: 1377-1381

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Reusable Liposome Array and Its application to Assay of Growth Hormone Related Peptides2010

    • Author(s)
      A.Shoji
    • Journal Title

      Anal.Bioanal.Chem. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of the L-Glutamate Level in Mouse Hippocampal Slices under Tetraethylammonium Chloride Stimulation as Measured with a Glass Capillary Sensor and a Patch Sensor2009

    • Author(s)
      T. Oka, Y. Tominaga, Y. Wakabayashi, A. Shoji, M. Sugawara
    • Journal Title

      Analytical Sciences

      Volume: 25 Issue: 3 Pages: 353-358

    • DOI

      10.2116/analsci.25.353

    • NAID

      130004441823

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing L-glutamate fluxes in acute brain slices with glutamate oxidase-immobilized slips2009

    • Author(s)
      W. Okumura, N. Moridera, E. Kanazawa, A. Shouji, A. Hirano-Iwata, M. Sugawara
    • Journal Title

      Anal. Biochem.

      Volume: 385 Issue: 2 Pages: 326-333

    • DOI

      10.1016/j.ab.2008.10.047

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of L-Glutamate Levels in Mouse Hippocampal Slices as Measured with a Glass Capillary Sensor and a Patch Sensor2009

    • Author(s)
      T.Oka
    • Journal Title

      Anal.Sci. 25

      Pages: 353-358

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] コステロール誘導体リポソームを用いるストレプトリジンOのナノポア形成能の制御とその応用2012

    • Author(s)
      東海林敦, 高辻龍太郎, 柿崎郁美, 小川祥二郎, 飯田隆, 菅原正雄
    • Organizer
      日本分析化学会第72回討論会
    • Place of Presentation
      鹿児島
    • Year and Date
      2012-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] グラミシジンチャネルの平面脂質二分子膜中での挙動とイムノセンサーへの応用2012

    • Author(s)
      西尾将人, 野澤桂一郎, 東海林敦, 菅原正雄
    • Organizer
      日本分析化学会第72回討論会
    • Place of Presentation
      鹿児島
    • Year and Date
      2012-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 脂質封入メソポーラスシリカのナノ細孔を用いる蛍光バイオセンシング法の基礎検討2012

    • Author(s)
      竹内亮, 野澤桂一郎, 東海林敦, 菅原正雄
    • Organizer
      日本分析化学会第72回討論会
    • Place of Presentation
      鹿児島
    • Year and Date
      2012-05-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 神経可塑性における放出グルタミン酸量の脳スライス内その場計測2012

    • Author(s)
      手塚竜太, 平野愛弓, 菅原正雄, 庭野道夫
    • Organizer
      2012年春季第59回応用物理学関係連合講演会
    • Place of Presentation
      東京
    • Year and Date
      2012-03-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 脂質膜を用いるバイオセンシング法の高度化と応用2011

    • Author(s)
      菅原正雄
    • Organizer
      日本分析化学会関東支部茨城地区技術交流会
    • Place of Presentation
      東海村
    • Year and Date
      2011-10-28
    • Related Report
      2011 Final Research Report
  • [Presentation] グラミシジンを包埋したイムノリポソーム凝集体によるサブスタンスPの超高感度イムノアッセイ2011

    • Author(s)
      阪本美里, 東海林敦, 菅原正雄
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] グルタミン酸濃度と興奮性シナプス後電位の同時計測:長期増強現象とグルタミン酸濃度の関係2011

    • Author(s)
      穂積志津子, 東海林敦, 菅原正雄
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 界面スライスを対象とする埋め込みパッチセンサーの開発2011

    • Author(s)
      相馬奈都美, 穂積志津子, 東海林敦, 菅原正雄
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 脂質二分子膜とチャンネルを用いる物質検知法2011

    • Author(s)
      菅原正雄
    • Organizer
      電気通信研究所共同プロジェクト研究会,第15回情報バイオトロニクス研究会
    • Place of Presentation
      仙台
    • Year and Date
      2011-01-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 電気で遊ぶ2010

    • Author(s)
      菅原正雄
    • Organizer
      第4回千葉県分析化学交流会
    • Place of Presentation
      東京
    • Year and Date
      2010-12-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 表面プラズモン共鳴法を用いたMatrix Metalloproteinase-9阻害活性測定法の構築2010

    • Author(s)
      壁谷充尭, 東海林敦, 野澤桂一郎, 菅原正雄
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 急性海馬スライスを電気刺激した際に放出されるグルタミン酸のリアルタイム計測2010

    • Author(s)
      穂積志津子, 池澤香奈, 堀江未恵子, 東海林敦, 平野愛弓, T. Bliss, 菅原正雄
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 海馬スライスの化学刺激に伴い放出されるL-グルタミン酸の領野間分布2010

    • Author(s)
      東海林敦, 金澤恵奈, 江口由夏, 野澤桂一郎, 菅原正雄
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 平面脂質二分子膜中のメソポーラスシリカへのレセプター修飾法2010

    • Author(s)
      野澤桂一郎, 那須朋大, 大島梓, 東海林敦, 平野愛弓, 庭野道夫, 菅原正雄
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス海馬内のグルタミン酸計測法の開発と応用2010

    • Author(s)
      菅原正雄
    • Organizer
      日本分析化学会関東支部新潟地区研究発表会
    • Place of Presentation
      新潟
    • Year and Date
      2010-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 化学刺激下の急性海馬スライスで放出されるグルタミン酸濃度の領野間分布の評価2010

    • Author(s)
      菅原正雄, 東海林敦, 野澤桂一郎, 千葉広美, 出口由佳莉, 金澤恵奈, 河合淳
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 平面脂質二分子膜中のMCM-41チャネルへのレセプターその場修飾法の検討2010

    • Author(s)
      野澤桂一郎, 大島梓, 東海林敦, 平野愛弓, 庭野道夫, 菅原正雄
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 再利用可能な蛍光リポソームアレイによるペプチドホルモンの同時計測2010

    • Author(s)
      東海林敦, 折田さやか, 杉本恵莉香, 野澤桂一郎, 柳田顕郎, 渋沢庸一, 菅原正雄
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 再利用可能な蛍光リポソームアレイを用いたペプチドホルモンの同時計測法2009

    • Author(s)
      東海林敦, 折田さやか, 杉本恵莉香, 野澤桂一郎, 柳田顕郎, 渋沢庸一, 菅原正雄
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      札幌
    • Year and Date
      2009-09-25
    • Related Report
      2011 Final Research Report
  • [Presentation] ナノ細孔内酵素反応の制御に基づく分子センシング法の基礎検討2009

    • Author(s)
      野澤桂一郎, 菅原正雄
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-29
    • Related Report
      2011 Final Research Report
  • [Book] A Glass Capillary-based Microsensor for L-Glutamate in in vitro Uses, Microsensors2011

    • Author(s)
      Masao Sugawara and Atushi Shoji
    • Publisher
      InTech
    • URL

      http://www.intechopen.com.

    • Related Report
      2011 Final Research Report
  • [Book] Microsensors2011

    • Author(s)
      M.Sugawara
    • Publisher
      InTech, Slovakia
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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