Reaction Mechanism of Heme Oxygenase
| Project/Area Number |
21350087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUI Toshitaka 東北大学, 多元物質科学研究所, 講師 (90323120)
UNO Masaki 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10359549)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | ヘム / 酸素活性化 / 結晶構造解析 / ベルドヘム / 反応機構 / 構造活性相関 / ヘム代謝 / メスバウアー |
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| Research Abstract |
Enzymatic heme degaradation by heme oxygenase, especially its rate-limiting ring opening step, have been studied in detail. Spectroscopic analysis reveals the formation of a ferryl species as a ring opening intermediate. Crystallographic study on an intermediate-enzyme complex combined with theoritical calculations finally elucidate the rate-limiting reaction. We also examined a novel heme cleavage reaction to clarify its mechanism and the new heme catabolites were succesufully detected from cultured mammalian cells.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Heme regulates B-cell differentiation, antibody class switch, and heme oxygenase-1 expression in B cell as a ligand of Bach22011
Author(s)
M. Watanabe-Matsui, A. Muto, T. Matsui, A. Itoh-Nakadai, O. Nakajima, K. Murayama, M. Yamamoto, M. Ikeda-Saito, K. Igarashi
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Journal Title
Blood
Volume: 117
Pages: 5438-5448
Related Report
Peer Reviewed
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[Journal Article] Binding and selectivity of the marine toxins neodysiherbaine A, and its synthetic analogues, to GluK1 and GIuK2 kainate receptors2011
Author(s)
M.Unno, M.Shinohara, K.Takayama, H.Tanaka, K.Teruya, K.Doh-ura, R.Sakai, M.Sasaki, M.Ikeda-Saito
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Journal Title
Journal of Molecular Biology
Volume: 413
Issue: 3
Pages: 667-683
DOI
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
M.Unno, et al.
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Journal Title
Nanohybridization of Organic-Inorganic Materials(Springer)
Pages: 193-217
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