| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Research Abstract |
Enzymatic heme degaradation by heme oxygenase, especially its rate-limiting ring opening step, have been studied in detail. Spectroscopic analysis reveals the formation of a ferryl species as a ring opening intermediate. Crystallographic study on an intermediate-enzyme complex combined with theoritical calculations finally elucidate the rate-limiting reaction. We also examined a novel heme cleavage reaction to clarify its mechanism and the new heme catabolites were succesufully detected from cultured mammalian cells.
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