ANALYSIS OF DYNAMIC BEHAVIOR OF RNA USING LIGHT-UP RNA MODULE AS A TAG
| Project/Area Number |
21350091
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Keywords | 転写 / RNA / 蛍光 / 蛍光モニタリング / BODIPY / アプタマー / トブラマイシン / 融合遺伝子 / カナマシシン / 抗生物質 / リボソーム / 抗生物質修飾酵素 / 大腸菌 / タグ / 融合RNA(DNA) / 蛍光色素 / アミノ糖 / SELEX / カナマイシン / 回転阻害 / ヘキスト / 光るRNAモジュール |
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| Research Abstract |
The fluorescence intensity of tobramycin-bearing phenyl-BODIPY as a probe increases upon interaction with tobramycin-aptamer, an RNA sequence that specifically binds to tobramycin. Transcription of a target gene (tRNA in this case) containing the aptamer sequence downstream thereof affords a fused (target-aptamer) RNA, the aptamer component of which can be detected by enhanced fluorescence, although no big, in the presence of the probe. In this way, the transcription of the target gene can be fluorescence monitored.
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Report
(5 results)
Research Products
(27 results)
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[Presentation] 抗生物質耐性菌の蛍光センシング2012
Author(s)
井出敬一朗・清水康映・山本達望・青山安宏
Organizer
第64回有機合成化学協会関東支部新潟シンポジウム
Place of Presentation
長岡技術科学大学、長岡、新潟
Year and Date
2012-12-01
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